Amino acid dipepetide frequency for Mycoplasma sp. OR1901

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.395AlaAla: 2.395 ± 0.149
0.156AlaCys: 0.156 ± 0.023
2.353AlaAsp: 2.353 ± 0.106
2.735AlaGlu: 2.735 ± 0.143
2.218AlaPhe: 2.218 ± 0.108
2.166AlaGly: 2.166 ± 0.12
0.656AlaHis: 0.656 ± 0.048
4.571AlaIle: 4.571 ± 0.186
5.757AlaLys: 5.757 ± 0.316
4.63AlaLeu: 4.63 ± 0.188
0.739AlaMet: 0.739 ± 0.053
3.64AlaAsn: 3.64 ± 0.195
1.034AlaPro: 1.034 ± 0.06
1.669AlaGln: 1.669 ± 0.103
1.753AlaArg: 1.753 ± 0.093
3.054AlaSer: 3.054 ± 0.121
3.113AlaThr: 3.113 ± 0.154
2.502AlaVal: 2.502 ± 0.113
0.378AlaTrp: 0.378 ± 0.042
1.766AlaTyr: 1.766 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.163CysAla: 0.163 ± 0.024
0.017CysCys: 0.017 ± 0.008
0.187CysAsp: 0.187 ± 0.033
0.139CysGlu: 0.139 ± 0.022
0.156CysPhe: 0.156 ± 0.025
0.226CysGly: 0.226 ± 0.025
0.069CysHis: 0.069 ± 0.017
0.17CysIle: 0.17 ± 0.025
0.149CysLys: 0.149 ± 0.02
0.222CysLeu: 0.222 ± 0.027
0.017CysMet: 0.017 ± 0.007
0.132CysAsn: 0.132 ± 0.027
0.066CysPro: 0.066 ± 0.016
0.083CysGln: 0.083 ± 0.017
0.073CysArg: 0.073 ± 0.016
0.295CysSer: 0.295 ± 0.039
0.097CysThr: 0.097 ± 0.018
0.142CysVal: 0.142 ± 0.023
0.021CysTrp: 0.021 ± 0.009
0.115CysTyr: 0.115 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.057AspAla: 3.057 ± 0.176
0.104AspCys: 0.104 ± 0.021
3.203AspAsp: 3.203 ± 0.124
5.056AspGlu: 5.056 ± 0.18
3.679AspPhe: 3.679 ± 0.13
2.808AspGly: 2.808 ± 0.127
0.701AspHis: 0.701 ± 0.054
4.963AspIle: 4.963 ± 0.136
6.812AspLys: 6.812 ± 0.216
6.011AspLeu: 6.011 ± 0.159
0.646AspMet: 0.646 ± 0.044
4.73AspAsn: 4.73 ± 0.166
1.471AspPro: 1.471 ± 0.074
2.443AspGln: 2.443 ± 0.123
1.551AspArg: 1.551 ± 0.065
4.047AspSer: 4.047 ± 0.144
2.71AspThr: 2.71 ± 0.108
3.304AspVal: 3.304 ± 0.107
0.472AspTrp: 0.472 ± 0.042
2.971AspTyr: 2.971 ± 0.098
0.0AspXaa: 0.0 ± 0.0
Glu
4.067GluAla: 4.067 ± 0.348
0.108GluCys: 0.108 ± 0.022
3.45GluAsp: 3.45 ± 0.125
6.143GluGlu: 6.143 ± 0.274
3.974GluPhe: 3.974 ± 0.117
2.894GluGly: 2.894 ± 0.097
0.958GluHis: 0.958 ± 0.065
7.652GluIle: 7.652 ± 0.213
8.336GluLys: 8.336 ± 0.242
8.055GluLeu: 8.055 ± 0.296
1.17GluMet: 1.17 ± 0.069
7.142GluAsn: 7.142 ± 0.203
1.406GluPro: 1.406 ± 0.09
2.967GluGln: 2.967 ± 0.2
2.048GluArg: 2.048 ± 0.097
4.112GluSer: 4.112 ± 0.115
3.932GluThr: 3.932 ± 0.124
4.158GluVal: 4.158 ± 0.152
0.566GluTrp: 0.566 ± 0.041
3.568GluTyr: 3.568 ± 0.127
0.0GluXaa: 0.0 ± 0.0
Phe
2.329PheAla: 2.329 ± 0.121
0.167PheCys: 0.167 ± 0.026
3.929PheAsp: 3.929 ± 0.159
3.856PheGlu: 3.856 ± 0.11
2.065PhePhe: 2.065 ± 0.12
2.617PheGly: 2.617 ± 0.148
0.493PheHis: 0.493 ± 0.048
4.65PheIle: 4.65 ± 0.169
5.317PheLys: 5.317 ± 0.169
4.269PheLeu: 4.269 ± 0.187
0.843PheMet: 0.843 ± 0.059
4.491PheAsn: 4.491 ± 0.144
1.038PhePro: 1.038 ± 0.058
1.131PheGln: 1.131 ± 0.051
1.343PheArg: 1.343 ± 0.068
3.519PheSer: 3.519 ± 0.131
2.631PheThr: 2.631 ± 0.131
2.856PheVal: 2.856 ± 0.117
0.427PheTrp: 0.427 ± 0.046
2.138PheTyr: 2.138 ± 0.109
0.0PheXaa: 0.0 ± 0.0
Gly
2.356GlyAla: 2.356 ± 0.108
0.125GlyCys: 0.125 ± 0.021
2.651GlyAsp: 2.651 ± 0.1
2.714GlyGlu: 2.714 ± 0.12
2.589GlyPhe: 2.589 ± 0.109
2.589GlyGly: 2.589 ± 0.134
0.816GlyHis: 0.816 ± 0.058
4.154GlyIle: 4.154 ± 0.176
3.842GlyLys: 3.842 ± 0.163
3.998GlyLeu: 3.998 ± 0.145
0.809GlyMet: 0.809 ± 0.056
2.714GlyAsn: 2.714 ± 0.106
0.822GlyPro: 0.822 ± 0.06
1.468GlyGln: 1.468 ± 0.081
1.471GlyArg: 1.471 ± 0.087
3.356GlySer: 3.356 ± 0.146
2.735GlyThr: 2.735 ± 0.135
2.818GlyVal: 2.818 ± 0.128
0.458GlyTrp: 0.458 ± 0.041
2.065GlyTyr: 2.065 ± 0.108
0.0GlyXaa: 0.0 ± 0.0
His
0.649HisAla: 0.649 ± 0.052
0.052HisCys: 0.052 ± 0.015
0.711HisAsp: 0.711 ± 0.056
0.829HisGlu: 0.829 ± 0.05
0.788HisPhe: 0.788 ± 0.054
0.649HisGly: 0.649 ± 0.057
0.26HisHis: 0.26 ± 0.046
1.117HisIle: 1.117 ± 0.06
1.229HisLys: 1.229 ± 0.06
1.281HisLeu: 1.281 ± 0.059
0.198HisMet: 0.198 ± 0.027
1.104HisAsn: 1.104 ± 0.061
0.507HisPro: 0.507 ± 0.037
0.43HisGln: 0.43 ± 0.035
0.351HisArg: 0.351 ± 0.04
0.854HisSer: 0.854 ± 0.067
0.621HisThr: 0.621 ± 0.045
0.639HisVal: 0.639 ± 0.054
0.118HisTrp: 0.118 ± 0.021
0.51HisTyr: 0.51 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
4.383IleAla: 4.383 ± 0.161
0.233IleCys: 0.233 ± 0.033
6.219IleAsp: 6.219 ± 0.138
7.069IleGlu: 7.069 ± 0.227
4.407IlePhe: 4.407 ± 0.198
3.925IleGly: 3.925 ± 0.176
1.128IleHis: 1.128 ± 0.073
7.857IleIle: 7.857 ± 0.262
9.62IleLys: 9.62 ± 0.212
7.628IleLeu: 7.628 ± 0.239
1.159IleMet: 1.159 ± 0.069
8.898IleAsn: 8.898 ± 0.286
2.447IlePro: 2.447 ± 0.135
2.655IleGln: 2.655 ± 0.104
2.332IleArg: 2.332 ± 0.092
6.837IleSer: 6.837 ± 0.182
4.831IleThr: 4.831 ± 0.149
4.779IleVal: 4.779 ± 0.145
0.649IleTrp: 0.649 ± 0.058
3.269IleTyr: 3.269 ± 0.149
0.0IleXaa: 0.0 ± 0.0
Lys
4.574LysAla: 4.574 ± 0.258
0.198LysCys: 0.198 ± 0.028
6.493LysAsp: 6.493 ± 0.208
9.634LysGlu: 9.634 ± 0.254
4.664LysPhe: 4.664 ± 0.121
3.616LysGly: 3.616 ± 0.136
1.371LysHis: 1.371 ± 0.072
10.463LysIle: 10.463 ± 0.213
9.721LysLys: 9.721 ± 0.243
9.787LysLeu: 9.787 ± 0.3
2.304LysMet: 2.304 ± 0.114
10.096LysAsn: 10.096 ± 0.246
2.422LysPro: 2.422 ± 0.128
3.63LysGln: 3.63 ± 0.153
2.988LysArg: 2.988 ± 0.13
5.792LysSer: 5.792 ± 0.134
6.07LysThr: 6.07 ± 0.16
5.719LysVal: 5.719 ± 0.157
0.888LysTrp: 0.888 ± 0.064
4.907LysTyr: 4.907 ± 0.136
0.0LysXaa: 0.0 ± 0.0
Leu
4.227LeuAla: 4.227 ± 0.142
0.246LeuCys: 0.246 ± 0.033
6.125LeuAsp: 6.125 ± 0.172
7.101LeuGlu: 7.101 ± 0.256
4.407LeuPhe: 4.407 ± 0.162
4.126LeuGly: 4.126 ± 0.15
1.076LeuHis: 1.076 ± 0.065
8.221LeuIle: 8.221 ± 0.222
9.71LeuLys: 9.71 ± 0.222
8.062LeuLeu: 8.062 ± 0.213
1.423LeuMet: 1.423 ± 0.075
9.006LeuAsn: 9.006 ± 0.272
2.495LeuPro: 2.495 ± 0.108
2.297LeuGln: 2.297 ± 0.107
2.762LeuArg: 2.762 ± 0.121
6.958LeuSer: 6.958 ± 0.16
4.834LeuThr: 4.834 ± 0.152
5.115LeuVal: 5.115 ± 0.147
0.625LeuTrp: 0.625 ± 0.046
2.964LeuTyr: 2.964 ± 0.113
0.0LeuXaa: 0.0 ± 0.0
Met
0.746MetAla: 0.746 ± 0.049
0.066MetCys: 0.066 ± 0.016
0.802MetAsp: 0.802 ± 0.065
0.875MetGlu: 0.875 ± 0.061
0.996MetPhe: 0.996 ± 0.066
0.673MetGly: 0.673 ± 0.057
0.205MetHis: 0.205 ± 0.028
1.34MetIle: 1.34 ± 0.081
1.725MetLys: 1.725 ± 0.082
1.402MetLeu: 1.402 ± 0.087
0.337MetMet: 0.337 ± 0.034
1.284MetAsn: 1.284 ± 0.068
0.531MetPro: 0.531 ± 0.06
0.444MetGln: 0.444 ± 0.042
0.51MetArg: 0.51 ± 0.049
1.111MetSer: 1.111 ± 0.06
0.795MetThr: 0.795 ± 0.056
0.854MetVal: 0.854 ± 0.054
0.163MetTrp: 0.163 ± 0.026
0.566MetTyr: 0.566 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
3.689AsnAla: 3.689 ± 0.191
0.142AsnCys: 0.142 ± 0.026
6.139AsnAsp: 6.139 ± 0.222
7.333AsnGlu: 7.333 ± 0.239
4.14AsnPhe: 4.14 ± 0.18
3.807AsnGly: 3.807 ± 0.112
0.94AsnHis: 0.94 ± 0.063
7.913AsnIle: 7.913 ± 0.224
9.936AsnLys: 9.936 ± 0.247
7.389AsnLeu: 7.389 ± 0.194
1.069AsnMet: 1.069 ± 0.076
9.429AsnAsn: 9.429 ± 0.329
2.447AsnPro: 2.447 ± 0.093
3.002AsnGln: 3.002 ± 0.133
2.287AsnArg: 2.287 ± 0.116
6.49AsnSer: 6.49 ± 0.22
4.48AsnThr: 4.48 ± 0.163
4.529AsnVal: 4.529 ± 0.143
0.757AsnTrp: 0.757 ± 0.052
3.762AsnTyr: 3.762 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
1.031ProAla: 1.031 ± 0.068
0.045ProCys: 0.045 ± 0.013
1.357ProAsp: 1.357 ± 0.077
2.131ProGlu: 2.131 ± 0.092
1.204ProPhe: 1.204 ± 0.082
1.329ProGly: 1.329 ± 0.093
0.441ProHis: 0.441 ± 0.046
2.041ProIle: 2.041 ± 0.112
2.592ProLys: 2.592 ± 0.131
1.909ProLeu: 1.909 ± 0.082
0.344ProMet: 0.344 ± 0.035
2.11ProAsn: 2.11 ± 0.085
0.396ProPro: 0.396 ± 0.049
0.791ProGln: 0.791 ± 0.053
0.642ProArg: 0.642 ± 0.052
1.815ProSer: 1.815 ± 0.084
1.593ProThr: 1.593 ± 0.087
1.34ProVal: 1.34 ± 0.093
0.271ProTrp: 0.271 ± 0.033
0.965ProTyr: 0.965 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
1.676GlnAla: 1.676 ± 0.11
0.052GlnCys: 0.052 ± 0.016
1.583GlnAsp: 1.583 ± 0.094
2.697GlnGlu: 2.697 ± 0.147
1.524GlnPhe: 1.524 ± 0.067
1.239GlnGly: 1.239 ± 0.073
0.347GlnHis: 0.347 ± 0.038
3.134GlnIle: 3.134 ± 0.113
3.661GlnLys: 3.661 ± 0.135
3.099GlnLeu: 3.099 ± 0.137
0.604GlnMet: 0.604 ± 0.049
2.998GlnAsn: 2.998 ± 0.119
0.788GlnPro: 0.788 ± 0.055
1.013GlnGln: 1.013 ± 0.064
1.131GlnArg: 1.131 ± 0.071
1.607GlnSer: 1.607 ± 0.067
1.829GlnThr: 1.829 ± 0.092
1.721GlnVal: 1.721 ± 0.081
0.267GlnTrp: 0.267 ± 0.032
1.232GlnTyr: 1.232 ± 0.074
0.0GlnXaa: 0.0 ± 0.0
Arg
1.454ArgAla: 1.454 ± 0.071
0.073ArgCys: 0.073 ± 0.015
1.78ArgAsp: 1.78 ± 0.079
2.308ArgGlu: 2.308 ± 0.103
1.288ArgPhe: 1.288 ± 0.074
1.312ArgGly: 1.312 ± 0.087
0.43ArgHis: 0.43 ± 0.037
2.78ArgIle: 2.78 ± 0.103
2.804ArgLys: 2.804 ± 0.112
2.638ArgLeu: 2.638 ± 0.134
0.569ArgMet: 0.569 ± 0.054
2.363ArgAsn: 2.363 ± 0.112
0.687ArgPro: 0.687 ± 0.054
0.934ArgGln: 0.934 ± 0.059
1.163ArgArg: 1.163 ± 0.074
1.548ArgSer: 1.548 ± 0.08
1.513ArgThr: 1.513 ± 0.089
1.749ArgVal: 1.749 ± 0.079
0.25ArgTrp: 0.25 ± 0.03
1.277ArgTyr: 1.277 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
2.683SerAla: 2.683 ± 0.102
0.278SerCys: 0.278 ± 0.034
3.831SerAsp: 3.831 ± 0.125
4.959SerGlu: 4.959 ± 0.17
3.62SerPhe: 3.62 ± 0.155
3.359SerGly: 3.359 ± 0.141
0.875SerHis: 0.875 ± 0.07
6.007SerIle: 6.007 ± 0.172
7.354SerLys: 7.354 ± 0.176
6.771SerLeu: 6.771 ± 0.18
1.065SerMet: 1.065 ± 0.073
6.087SerAsn: 6.087 ± 0.174
1.294SerPro: 1.294 ± 0.074
2.152SerGln: 2.152 ± 0.092
1.895SerArg: 1.895 ± 0.075
4.744SerSer: 4.744 ± 0.143
3.811SerThr: 3.811 ± 0.12
3.533SerVal: 3.533 ± 0.128
0.59SerTrp: 0.59 ± 0.045
2.808SerTyr: 2.808 ± 0.126
0.0SerXaa: 0.0 ± 0.0
Thr
2.093ThrAla: 2.093 ± 0.092
0.121ThrCys: 0.121 ± 0.024
2.908ThrAsp: 2.908 ± 0.119
3.672ThrGlu: 3.672 ± 0.164
2.915ThrPhe: 2.915 ± 0.123
2.537ThrGly: 2.537 ± 0.134
0.829ThrHis: 0.829 ± 0.052
4.775ThrIle: 4.775 ± 0.166
5.931ThrLys: 5.931 ± 0.163
5.195ThrLeu: 5.195 ± 0.135
0.666ThrMet: 0.666 ± 0.048
4.852ThrAsn: 4.852 ± 0.153
1.798ThrPro: 1.798 ± 0.113
1.819ThrGln: 1.819 ± 0.095
1.583ThrArg: 1.583 ± 0.088
3.894ThrSer: 3.894 ± 0.105
3.481ThrThr: 3.481 ± 0.146
2.769ThrVal: 2.769 ± 0.118
0.531ThrTrp: 0.531 ± 0.044
2.159ThrTyr: 2.159 ± 0.09
0.0ThrXaa: 0.0 ± 0.0
Val
3.241ValAla: 3.241 ± 0.118
0.187ValCys: 0.187 ± 0.027
3.807ValAsp: 3.807 ± 0.108
4.296ValGlu: 4.296 ± 0.147
2.78ValPhe: 2.78 ± 0.12
2.565ValGly: 2.565 ± 0.129
0.663ValHis: 0.663 ± 0.055
4.449ValIle: 4.449 ± 0.157
5.518ValLys: 5.518 ± 0.146
4.692ValLeu: 4.692 ± 0.141
0.829ValMet: 0.829 ± 0.055
4.126ValAsn: 4.126 ± 0.123
1.454ValPro: 1.454 ± 0.081
1.569ValGln: 1.569 ± 0.079
1.551ValArg: 1.551 ± 0.08
3.967ValSer: 3.967 ± 0.133
3.005ValThr: 3.005 ± 0.116
3.835ValVal: 3.835 ± 0.144
0.469ValTrp: 0.469 ± 0.05
2.152ValTyr: 2.152 ± 0.095
0.0ValXaa: 0.0 ± 0.0
Trp
0.448TrpAla: 0.448 ± 0.035
0.038TrpCys: 0.038 ± 0.012
0.493TrpAsp: 0.493 ± 0.042
0.566TrpGlu: 0.566 ± 0.044
0.489TrpPhe: 0.489 ± 0.051
0.42TrpGly: 0.42 ± 0.047
0.111TrpHis: 0.111 ± 0.02
0.757TrpIle: 0.757 ± 0.051
0.701TrpLys: 0.701 ± 0.051
0.704TrpLeu: 0.704 ± 0.048
0.16TrpMet: 0.16 ± 0.023
0.802TrpAsn: 0.802 ± 0.059
0.184TrpPro: 0.184 ± 0.02
0.187TrpGln: 0.187 ± 0.025
0.274TrpArg: 0.274 ± 0.034
0.621TrpSer: 0.621 ± 0.05
0.521TrpThr: 0.521 ± 0.046
0.482TrpVal: 0.482 ± 0.041
0.097TrpTrp: 0.097 ± 0.019
0.392TrpTyr: 0.392 ± 0.042
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.023TyrAla: 2.023 ± 0.091
0.132TyrCys: 0.132 ± 0.022
2.721TyrAsp: 2.721 ± 0.113
2.592TyrGlu: 2.592 ± 0.102
2.204TyrPhe: 2.204 ± 0.114
1.694TyrGly: 1.694 ± 0.096
0.528TyrHis: 0.528 ± 0.048
3.311TyrIle: 3.311 ± 0.13
4.623TyrLys: 4.623 ± 0.16
4.043TyrLeu: 4.043 ± 0.143
0.469TyrMet: 0.469 ± 0.04
3.675TyrAsn: 3.675 ± 0.118
1.058TyrPro: 1.058 ± 0.07
1.517TyrGln: 1.517 ± 0.073
1.211TyrArg: 1.211 ± 0.086
3.096TyrSer: 3.096 ± 0.128
1.926TyrThr: 1.926 ± 0.101
2.311TyrVal: 2.311 ± 0.103
0.451TyrTrp: 0.451 ± 0.049
1.735TyrTyr: 1.735 ± 0.104
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 651 proteins (288149 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski