Allokutzneria albata
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7717 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0BKY7|A0A1H0BKY7_9PSEU GDSL-like Lipase/Acylhydrolase family protein OS=Allokutzneria albata OX=211114 GN=SAMN04489726_6723 PE=4 SV=1
MM1 pKa = 7.6 AVQLDD6 pKa = 3.91 AQLLEE11 pKa = 4.39 ILACPGDD18 pKa = 3.71 DD19 pKa = 4.02 HH20 pKa = 6.86 GALVTGTPEE29 pKa = 4.26 DD30 pKa = 3.55 TDD32 pKa = 4.33 ADD34 pKa = 4.31 YY35 pKa = 8.74 LTCTACARR43 pKa = 11.84 RR44 pKa = 11.84 YY45 pKa = 8.61 PVRR48 pKa = 11.84 DD49 pKa = 4.63 GIPVLLLEE57 pKa = 4.47 EE58 pKa = 5.01 AEE60 pKa = 4.66 GGPEE64 pKa = 4.16 DD65 pKa = 4.2 SAKK68 pKa = 10.85 AA69 pKa = 3.44
Molecular weight: 7.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.923
IPC_protein 3.834
Toseland 3.643
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.961
Patrickios 1.901
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A1G9ZJZ8|A0A1G9ZJZ8_9PSEU Transcriptional regulator PadR family OS=Allokutzneria albata OX=211114 GN=SAMN04489726_5553 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.79 HH17 pKa = 5.67 RR18 pKa = 11.84 KK19 pKa = 8.31 LLRR22 pKa = 11.84 KK23 pKa = 7.53 TRR25 pKa = 11.84 HH26 pKa = 5.15 ARR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 8.68 QGKK33 pKa = 8.75
Molecular weight: 4.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7717
0
7717
2556809
29
22598
331.3
35.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.011 ± 0.036
0.858 ± 0.011
5.831 ± 0.025
5.823 ± 0.03
2.894 ± 0.014
9.015 ± 0.029
2.241 ± 0.012
3.348 ± 0.016
2.182 ± 0.022
10.688 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.775 ± 0.011
1.931 ± 0.018
5.841 ± 0.023
2.742 ± 0.019
8.056 ± 0.03
5.277 ± 0.018
5.844 ± 0.024
9.143 ± 0.027
1.575 ± 0.012
1.925 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here