Clostridioides phage LIBA2945

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2R2ZHC2|A0A2R2ZHC2_9CAUD Uncharacterized protein OS=Clostridioides phage LIBA2945 OX=2069615 GN=LIBA2945_00129 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 5.16GEE4 pKa = 4.33VYY6 pKa = 10.71NYY8 pKa = 10.12LDD10 pKa = 4.39CGSYY14 pKa = 9.72TYY16 pKa = 10.98SGLGKK21 pKa = 10.41DD22 pKa = 3.61YY23 pKa = 11.23DD24 pKa = 3.63VDD26 pKa = 3.69INIVFDD32 pKa = 4.09VIKK35 pKa = 10.62INSEE39 pKa = 3.58EE40 pKa = 4.17DD41 pKa = 3.29YY42 pKa = 11.42KK43 pKa = 11.5SIIKK47 pKa = 10.39IKK49 pKa = 10.47DD50 pKa = 3.02IEE52 pKa = 4.18LLL54 pKa = 3.85

Molecular weight:
6.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2R2ZHB7|A0A2R2ZHB7_9CAUD DNA ligase OS=Clostridioides phage LIBA2945 OX=2069615 GN=ligA PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 7.78THH5 pKa = 5.93RR6 pKa = 11.84VKK8 pKa = 11.18LNLTRR13 pKa = 11.84TQFEE17 pKa = 5.08LIRR20 pKa = 11.84EE21 pKa = 4.18KK22 pKa = 11.06QMEE25 pKa = 4.33SANCWNHH32 pKa = 5.2IVNLSKK38 pKa = 10.6EE39 pKa = 4.37YY40 pKa = 10.77YY41 pKa = 9.71FEE43 pKa = 4.57HH44 pKa = 6.34KK45 pKa = 10.07QWIKK49 pKa = 11.18KK50 pKa = 10.17NDD52 pKa = 3.18IQKK55 pKa = 10.47SIKK58 pKa = 10.19GKK60 pKa = 10.73YY61 pKa = 8.06NLHH64 pKa = 6.09SQTIQAISDD73 pKa = 3.53KK74 pKa = 10.74FDD76 pKa = 4.05ANRR79 pKa = 11.84KK80 pKa = 7.19TISEE84 pKa = 4.09LRR86 pKa = 11.84KK87 pKa = 10.19KK88 pKa = 11.04GNTKK92 pKa = 10.42AKK94 pKa = 10.39YY95 pKa = 9.07PYY97 pKa = 8.39KK98 pKa = 9.96TKK100 pKa = 10.56KK101 pKa = 10.45FYY103 pKa = 10.37IIPFKK108 pKa = 11.01ASAINRR114 pKa = 11.84NAKK117 pKa = 10.33GNLKK121 pKa = 10.63LSMSKK126 pKa = 10.52GRR128 pKa = 11.84YY129 pKa = 8.96LEE131 pKa = 4.96LDD133 pKa = 3.71FNVEE137 pKa = 4.23NIKK140 pKa = 9.02TAEE143 pKa = 4.03IVWRR147 pKa = 11.84NGYY150 pKa = 8.43YY151 pKa = 10.52LYY153 pKa = 10.03YY154 pKa = 10.73TFDD157 pKa = 3.46NEE159 pKa = 4.61LNGVVAKK166 pKa = 8.43GTNTVGVDD174 pKa = 3.1LGEE177 pKa = 4.0IHH179 pKa = 7.23SIASVTNEE187 pKa = 3.64GVGLILSNRR196 pKa = 11.84EE197 pKa = 3.44GRR199 pKa = 11.84SIKK202 pKa = 8.65QFRR205 pKa = 11.84NKK207 pKa = 9.01MYY209 pKa = 10.48AYY211 pKa = 9.66ISKK214 pKa = 10.49RR215 pKa = 11.84LSKK218 pKa = 10.26CKK220 pKa = 10.09KK221 pKa = 9.93GSRR224 pKa = 11.84QSKK227 pKa = 9.92KK228 pKa = 8.94LWRR231 pKa = 11.84LKK233 pKa = 10.89NKK235 pKa = 9.32IRR237 pKa = 11.84SKK239 pKa = 10.1TDD241 pKa = 3.02NQLMNLYY248 pKa = 9.82HH249 pKa = 4.79QTTRR253 pKa = 11.84KK254 pKa = 10.0FIDD257 pKa = 3.68FCVEE261 pKa = 3.78QKK263 pKa = 10.43VCEE266 pKa = 4.08IVLGDD271 pKa = 3.45IKK273 pKa = 11.06GVEE276 pKa = 4.12KK277 pKa = 9.91DD278 pKa = 3.33TKK280 pKa = 10.24KK281 pKa = 10.56KK282 pKa = 10.37KK283 pKa = 10.05RR284 pKa = 11.84LNRR287 pKa = 11.84VNRR290 pKa = 11.84QKK292 pKa = 10.47ISQMEE297 pKa = 3.87YY298 pKa = 11.1GRR300 pKa = 11.84IKK302 pKa = 10.83DD303 pKa = 3.82YY304 pKa = 11.1IKK306 pKa = 11.16YY307 pKa = 9.32KK308 pKa = 10.69AKK310 pKa = 10.21EE311 pKa = 3.61QGIEE315 pKa = 3.96VKK317 pKa = 10.2LVKK320 pKa = 10.23EE321 pKa = 4.49NYY323 pKa = 8.57TSQTCPKK330 pKa = 9.29CSKK333 pKa = 9.45KK334 pKa = 10.05HH335 pKa = 5.58KK336 pKa = 8.34PTGRR340 pKa = 11.84TYY342 pKa = 11.24SCTCGYY348 pKa = 7.72KK349 pKa = 7.15THH351 pKa = 7.08RR352 pKa = 11.84DD353 pKa = 3.06IVGAWNILNKK363 pKa = 8.84KK364 pKa = 8.62HH365 pKa = 6.83KK366 pKa = 10.23YY367 pKa = 9.52GLVDD371 pKa = 3.73FRR373 pKa = 11.84INHH376 pKa = 5.56KK377 pKa = 10.56QPINLKK383 pKa = 10.05VSTVV387 pKa = 3.02

Molecular weight:
45.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

37778

36

2022

219.6

25.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.65 ± 0.194

1.459 ± 0.097

6.692 ± 0.156

8.484 ± 0.224

3.886 ± 0.124

4.95 ± 0.277

1.313 ± 0.078

10.046 ± 0.231

10.871 ± 0.277

8.484 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.112

8.129 ± 0.227

1.705 ± 0.073

2.79 ± 0.174

3.513 ± 0.145

6.019 ± 0.151

4.913 ± 0.223

4.95 ± 0.166

0.813 ± 0.052

4.921 ± 0.187

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski