Clostridioides phage LIBA2945
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R2ZHC2|A0A2R2ZHC2_9CAUD Uncharacterized protein OS=Clostridioides phage LIBA2945 OX=2069615 GN=LIBA2945_00129 PE=4 SV=1
MM1 pKa = 7.38 EE2 pKa = 5.16 GEE4 pKa = 4.33 VYY6 pKa = 10.71 NYY8 pKa = 10.12 LDD10 pKa = 4.39 CGSYY14 pKa = 9.72 TYY16 pKa = 10.98 SGLGKK21 pKa = 10.41 DD22 pKa = 3.61 YY23 pKa = 11.23 DD24 pKa = 3.63 VDD26 pKa = 3.69 INIVFDD32 pKa = 4.09 VIKK35 pKa = 10.62 INSEE39 pKa = 3.58 EE40 pKa = 4.17 DD41 pKa = 3.29 YY42 pKa = 11.42 KK43 pKa = 11.5 SIIKK47 pKa = 10.39 IKK49 pKa = 10.47 DD50 pKa = 3.02 IEE52 pKa = 4.18 LLL54 pKa = 3.85
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.822
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.745
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.783
Grimsley 3.668
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.266
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.062
Patrickios 0.782
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>tr|A0A2R2ZHB7|A0A2R2ZHB7_9CAUD DNA ligase OS=Clostridioides phage LIBA2945 OX=2069615 GN=ligA PE=4 SV=1
MM1 pKa = 7.57 IKK3 pKa = 7.78 THH5 pKa = 5.93 RR6 pKa = 11.84 VKK8 pKa = 11.18 LNLTRR13 pKa = 11.84 TQFEE17 pKa = 5.08 LIRR20 pKa = 11.84 EE21 pKa = 4.18 KK22 pKa = 11.06 QMEE25 pKa = 4.33 SANCWNHH32 pKa = 5.2 IVNLSKK38 pKa = 10.6 EE39 pKa = 4.37 YY40 pKa = 10.77 YY41 pKa = 9.71 FEE43 pKa = 4.57 HH44 pKa = 6.34 KK45 pKa = 10.07 QWIKK49 pKa = 11.18 KK50 pKa = 10.17 NDD52 pKa = 3.18 IQKK55 pKa = 10.47 SIKK58 pKa = 10.19 GKK60 pKa = 10.73 YY61 pKa = 8.06 NLHH64 pKa = 6.09 SQTIQAISDD73 pKa = 3.53 KK74 pKa = 10.74 FDD76 pKa = 4.05 ANRR79 pKa = 11.84 KK80 pKa = 7.19 TISEE84 pKa = 4.09 LRR86 pKa = 11.84 KK87 pKa = 10.19 KK88 pKa = 11.04 GNTKK92 pKa = 10.42 AKK94 pKa = 10.39 YY95 pKa = 9.07 PYY97 pKa = 8.39 KK98 pKa = 9.96 TKK100 pKa = 10.56 KK101 pKa = 10.45 FYY103 pKa = 10.37 IIPFKK108 pKa = 11.01 ASAINRR114 pKa = 11.84 NAKK117 pKa = 10.33 GNLKK121 pKa = 10.63 LSMSKK126 pKa = 10.52 GRR128 pKa = 11.84 YY129 pKa = 8.96 LEE131 pKa = 4.96 LDD133 pKa = 3.71 FNVEE137 pKa = 4.23 NIKK140 pKa = 9.02 TAEE143 pKa = 4.03 IVWRR147 pKa = 11.84 NGYY150 pKa = 8.43 YY151 pKa = 10.52 LYY153 pKa = 10.03 YY154 pKa = 10.73 TFDD157 pKa = 3.46 NEE159 pKa = 4.61 LNGVVAKK166 pKa = 8.43 GTNTVGVDD174 pKa = 3.1 LGEE177 pKa = 4.0 IHH179 pKa = 7.23 SIASVTNEE187 pKa = 3.64 GVGLILSNRR196 pKa = 11.84 EE197 pKa = 3.44 GRR199 pKa = 11.84 SIKK202 pKa = 8.65 QFRR205 pKa = 11.84 NKK207 pKa = 9.01 MYY209 pKa = 10.48 AYY211 pKa = 9.66 ISKK214 pKa = 10.49 RR215 pKa = 11.84 LSKK218 pKa = 10.26 CKK220 pKa = 10.09 KK221 pKa = 9.93 GSRR224 pKa = 11.84 QSKK227 pKa = 9.92 KK228 pKa = 8.94 LWRR231 pKa = 11.84 LKK233 pKa = 10.89 NKK235 pKa = 9.32 IRR237 pKa = 11.84 SKK239 pKa = 10.1 TDD241 pKa = 3.02 NQLMNLYY248 pKa = 9.82 HH249 pKa = 4.79 QTTRR253 pKa = 11.84 KK254 pKa = 10.0 FIDD257 pKa = 3.68 FCVEE261 pKa = 3.78 QKK263 pKa = 10.43 VCEE266 pKa = 4.08 IVLGDD271 pKa = 3.45 IKK273 pKa = 11.06 GVEE276 pKa = 4.12 KK277 pKa = 9.91 DD278 pKa = 3.33 TKK280 pKa = 10.24 KK281 pKa = 10.56 KK282 pKa = 10.37 KK283 pKa = 10.05 RR284 pKa = 11.84 LNRR287 pKa = 11.84 VNRR290 pKa = 11.84 QKK292 pKa = 10.47 ISQMEE297 pKa = 3.87 YY298 pKa = 11.1 GRR300 pKa = 11.84 IKK302 pKa = 10.83 DD303 pKa = 3.82 YY304 pKa = 11.1 IKK306 pKa = 11.16 YY307 pKa = 9.32 KK308 pKa = 10.69 AKK310 pKa = 10.21 EE311 pKa = 3.61 QGIEE315 pKa = 3.96 VKK317 pKa = 10.2 LVKK320 pKa = 10.23 EE321 pKa = 4.49 NYY323 pKa = 8.57 TSQTCPKK330 pKa = 9.29 CSKK333 pKa = 9.45 KK334 pKa = 10.05 HH335 pKa = 5.58 KK336 pKa = 8.34 PTGRR340 pKa = 11.84 TYY342 pKa = 11.24 SCTCGYY348 pKa = 7.72 KK349 pKa = 7.15 THH351 pKa = 7.08 RR352 pKa = 11.84 DD353 pKa = 3.06 IVGAWNILNKK363 pKa = 8.84 KK364 pKa = 8.62 HH365 pKa = 6.83 KK366 pKa = 10.23 YY367 pKa = 9.52 GLVDD371 pKa = 3.73 FRR373 pKa = 11.84 INHH376 pKa = 5.56 KK377 pKa = 10.56 QPINLKK383 pKa = 10.05 VSTVV387 pKa = 3.02
Molecular weight: 45.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.133
IPC2_protein 9.487
IPC_protein 9.385
Toseland 10.277
ProMoST 9.838
Dawson 10.409
Bjellqvist 10.028
Wikipedia 10.54
Rodwell 11.067
Grimsley 10.452
Solomon 10.423
Lehninger 10.394
Nozaki 10.262
DTASelect 10.028
Thurlkill 10.277
EMBOSS 10.657
Sillero 10.321
Patrickios 10.745
IPC_peptide 10.423
IPC2_peptide 8.595
IPC2.peptide.svr19 8.473
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
172
0
172
37778
36
2022
219.6
25.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.65 ± 0.194
1.459 ± 0.097
6.692 ± 0.156
8.484 ± 0.224
3.886 ± 0.124
4.95 ± 0.277
1.313 ± 0.078
10.046 ± 0.231
10.871 ± 0.277
8.484 ± 0.175
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.414 ± 0.112
8.129 ± 0.227
1.705 ± 0.073
2.79 ± 0.174
3.513 ± 0.145
6.019 ± 0.151
4.913 ± 0.223
4.95 ± 0.166
0.813 ± 0.052
4.921 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here