Amino acid dipepetide frequency for Clostridioides phage LIBA2945

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.085AlaAla: 1.085 ± 0.171
0.609AlaCys: 0.609 ± 0.134
2.038AlaAsp: 2.038 ± 0.248
3.044AlaGlu: 3.044 ± 0.34
1.721AlaPhe: 1.721 ± 0.252
1.774AlaGly: 1.774 ± 0.303
0.397AlaHis: 0.397 ± 0.123
4.288AlaIle: 4.288 ± 0.337
4.315AlaLys: 4.315 ± 0.44
2.859AlaLeu: 2.859 ± 0.331
0.715AlaMet: 0.715 ± 0.141
2.541AlaAsn: 2.541 ± 0.254
0.503AlaPro: 0.503 ± 0.163
0.9AlaGln: 0.9 ± 0.212
1.35AlaArg: 1.35 ± 0.213
2.197AlaSer: 2.197 ± 0.303
2.647AlaThr: 2.647 ± 0.284
1.191AlaVal: 1.191 ± 0.182
0.344AlaTrp: 0.344 ± 0.095
1.985AlaTyr: 1.985 ± 0.217
0.0AlaXaa: 0.0 ± 0.0
Cys
0.424CysAla: 0.424 ± 0.109
0.159CysCys: 0.159 ± 0.063
1.244CysAsp: 1.244 ± 0.206
1.085CysGlu: 1.085 ± 0.164
0.529CysPhe: 0.529 ± 0.113
1.588CysGly: 1.588 ± 0.479
0.397CysHis: 0.397 ± 0.096
1.35CysIle: 1.35 ± 0.182
1.641CysLys: 1.641 ± 0.24
0.768CysLeu: 0.768 ± 0.13
0.503CysMet: 0.503 ± 0.104
1.059CysAsn: 1.059 ± 0.178
0.318CysPro: 0.318 ± 0.094
0.238CysGln: 0.238 ± 0.074
0.503CysArg: 0.503 ± 0.108
0.662CysSer: 0.662 ± 0.123
0.662CysThr: 0.662 ± 0.12
0.556CysVal: 0.556 ± 0.127
0.132CysTrp: 0.132 ± 0.06
0.768CysTyr: 0.768 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
2.224AspAla: 2.224 ± 0.283
1.006AspCys: 1.006 ± 0.162
3.706AspAsp: 3.706 ± 0.314
5.638AspGlu: 5.638 ± 0.471
2.7AspPhe: 2.7 ± 0.241
3.521AspGly: 3.521 ± 0.312
0.609AspHis: 0.609 ± 0.127
7.65AspIle: 7.65 ± 0.421
7.2AspLys: 7.2 ± 0.451
5.903AspLeu: 5.903 ± 0.467
1.959AspMet: 1.959 ± 0.271
5.082AspAsn: 5.082 ± 0.442
0.476AspPro: 0.476 ± 0.112
0.609AspGln: 0.609 ± 0.127
2.594AspArg: 2.594 ± 0.254
3.759AspSer: 3.759 ± 0.328
3.732AspThr: 3.732 ± 0.358
4.288AspVal: 4.288 ± 0.316
0.529AspTrp: 0.529 ± 0.121
3.732AspTyr: 3.732 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
3.018GluAla: 3.018 ± 0.411
1.271GluCys: 1.271 ± 0.222
5.347GluAsp: 5.347 ± 0.42
7.835GluGlu: 7.835 ± 0.646
3.229GluPhe: 3.229 ± 0.255
3.441GluGly: 3.441 ± 0.379
1.032GluHis: 1.032 ± 0.149
9.0GluIle: 9.0 ± 0.493
8.762GluLys: 8.762 ± 0.53
9.159GluLeu: 9.159 ± 0.51
2.409GluMet: 2.409 ± 0.285
7.253GluAsn: 7.253 ± 0.412
0.874GluPro: 0.874 ± 0.162
2.779GluGln: 2.779 ± 0.302
2.621GluArg: 2.621 ± 0.285
3.732GluSer: 3.732 ± 0.325
3.044GluThr: 3.044 ± 0.355
4.844GluVal: 4.844 ± 0.361
0.794GluTrp: 0.794 ± 0.143
4.394GluTyr: 4.394 ± 0.413
0.0GluXaa: 0.0 ± 0.0
Phe
1.085PheAla: 1.085 ± 0.164
0.635PheCys: 0.635 ± 0.111
3.097PheAsp: 3.097 ± 0.281
3.362PheGlu: 3.362 ± 0.426
1.244PhePhe: 1.244 ± 0.183
1.668PheGly: 1.668 ± 0.2
0.265PheHis: 0.265 ± 0.096
3.706PheIle: 3.706 ± 0.288
4.553PheLys: 4.553 ± 0.344
3.018PheLeu: 3.018 ± 0.284
0.847PheMet: 0.847 ± 0.148
3.468PheAsn: 3.468 ± 0.362
0.688PhePro: 0.688 ± 0.167
1.218PheGln: 1.218 ± 0.172
1.35PheArg: 1.35 ± 0.19
2.541PheSer: 2.541 ± 0.24
2.118PheThr: 2.118 ± 0.5
1.827PheVal: 1.827 ± 0.233
0.291PheTrp: 0.291 ± 0.08
1.879PheTyr: 1.879 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
1.985GlyAla: 1.985 ± 0.289
0.688GlyCys: 0.688 ± 0.172
3.124GlyAsp: 3.124 ± 0.333
3.865GlyGlu: 3.865 ± 0.358
2.568GlyPhe: 2.568 ± 0.255
3.044GlyGly: 3.044 ± 0.584
0.768GlyHis: 0.768 ± 0.155
4.235GlyIle: 4.235 ± 0.403
5.003GlyLys: 5.003 ± 0.389
4.5GlyLeu: 4.5 ± 0.488
1.138GlyMet: 1.138 ± 0.181
3.415GlyAsn: 3.415 ± 0.397
0.0GlyPro: 0.0 ± 0.0
1.747GlyGln: 1.747 ± 0.425
1.879GlyArg: 1.879 ± 0.36
2.938GlySer: 2.938 ± 0.309
2.462GlyThr: 2.462 ± 0.256
2.568GlyVal: 2.568 ± 0.253
0.476GlyTrp: 0.476 ± 0.112
3.097GlyTyr: 3.097 ± 0.284
0.0GlyXaa: 0.0 ± 0.0
His
0.503HisAla: 0.503 ± 0.122
0.291HisCys: 0.291 ± 0.084
0.662HisAsp: 0.662 ± 0.119
0.847HisGlu: 0.847 ± 0.134
0.662HisPhe: 0.662 ± 0.149
0.874HisGly: 0.874 ± 0.191
0.185HisHis: 0.185 ± 0.066
1.271HisIle: 1.271 ± 0.205
1.429HisLys: 1.429 ± 0.227
0.9HisLeu: 0.9 ± 0.141
0.212HisMet: 0.212 ± 0.077
1.138HisAsn: 1.138 ± 0.166
0.318HisPro: 0.318 ± 0.095
0.291HisGln: 0.291 ± 0.084
0.424HisArg: 0.424 ± 0.11
0.979HisSer: 0.979 ± 0.15
0.874HisThr: 0.874 ± 0.141
0.529HisVal: 0.529 ± 0.136
0.106HisTrp: 0.106 ± 0.052
0.635HisTyr: 0.635 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
3.918IleAla: 3.918 ± 0.353
1.403IleCys: 1.403 ± 0.185
7.915IleAsp: 7.915 ± 0.435
9.159IleGlu: 9.159 ± 0.506
3.229IlePhe: 3.229 ± 0.342
4.209IleGly: 4.209 ± 0.346
1.376IleHis: 1.376 ± 0.203
9.0IleIle: 9.0 ± 0.666
11.78IleLys: 11.78 ± 0.676
8.471IleLeu: 8.471 ± 0.573
1.535IleMet: 1.535 ± 0.174
8.921IleAsn: 8.921 ± 0.589
2.594IlePro: 2.594 ± 0.245
3.15IleGln: 3.15 ± 0.291
3.441IleArg: 3.441 ± 0.327
7.068IleSer: 7.068 ± 0.518
4.5IleThr: 4.5 ± 0.324
4.288IleVal: 4.288 ± 0.409
0.635IleTrp: 0.635 ± 0.132
3.865IleTyr: 3.865 ± 0.389
0.0IleXaa: 0.0 ± 0.0
Lys
4.209LysAla: 4.209 ± 0.35
1.535LysCys: 1.535 ± 0.238
7.359LysAsp: 7.359 ± 0.393
11.833LysGlu: 11.833 ± 0.574
4.262LysPhe: 4.262 ± 0.384
4.368LysGly: 4.368 ± 0.373
1.535LysHis: 1.535 ± 0.229
10.668LysIle: 10.668 ± 0.535
10.774LysLys: 10.774 ± 0.716
8.868LysLeu: 8.868 ± 0.509
2.965LysMet: 2.965 ± 0.295
8.365LysAsn: 8.365 ± 0.513
1.429LysPro: 1.429 ± 0.165
3.335LysGln: 3.335 ± 0.42
3.653LysArg: 3.653 ± 0.402
5.48LysSer: 5.48 ± 0.395
5.427LysThr: 5.427 ± 0.394
5.374LysVal: 5.374 ± 0.389
0.768LysTrp: 0.768 ± 0.132
6.485LysTyr: 6.485 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
3.362LeuAla: 3.362 ± 0.422
0.953LeuCys: 0.953 ± 0.176
6.512LeuAsp: 6.512 ± 0.538
7.835LeuGlu: 7.835 ± 0.409
2.541LeuPhe: 2.541 ± 0.281
4.844LeuGly: 4.844 ± 0.676
1.191LeuHis: 1.191 ± 0.204
7.491LeuIle: 7.491 ± 0.439
10.165LeuLys: 10.165 ± 0.598
6.035LeuLeu: 6.035 ± 0.392
1.694LeuMet: 1.694 ± 0.216
7.28LeuAsn: 7.28 ± 0.431
1.747LeuPro: 1.747 ± 0.214
2.303LeuGln: 2.303 ± 0.458
3.282LeuArg: 3.282 ± 0.325
5.532LeuSer: 5.532 ± 0.366
4.024LeuThr: 4.024 ± 0.293
3.415LeuVal: 3.415 ± 0.266
0.662LeuTrp: 0.662 ± 0.13
3.971LeuTyr: 3.971 ± 0.503
0.0LeuXaa: 0.0 ± 0.0
Met
1.112MetAla: 1.112 ± 0.171
0.397MetCys: 0.397 ± 0.102
1.8MetAsp: 1.8 ± 0.249
2.065MetGlu: 2.065 ± 0.256
0.874MetPhe: 0.874 ± 0.157
0.688MetGly: 0.688 ± 0.162
0.344MetHis: 0.344 ± 0.096
2.303MetIle: 2.303 ± 0.212
2.488MetLys: 2.488 ± 0.243
1.853MetLeu: 1.853 ± 0.214
0.741MetMet: 0.741 ± 0.146
2.065MetAsn: 2.065 ± 0.268
0.424MetPro: 0.424 ± 0.108
0.741MetGln: 0.741 ± 0.143
0.979MetArg: 0.979 ± 0.156
1.879MetSer: 1.879 ± 0.204
1.006MetThr: 1.006 ± 0.184
1.059MetVal: 1.059 ± 0.138
0.132MetTrp: 0.132 ± 0.058
1.191MetTyr: 1.191 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
2.329AsnAla: 2.329 ± 0.291
1.218AsnCys: 1.218 ± 0.159
4.156AsnAsp: 4.156 ± 0.36
6.459AsnGlu: 6.459 ± 0.396
2.488AsnPhe: 2.488 ± 0.239
3.918AsnGly: 3.918 ± 0.367
1.059AsnHis: 1.059 ± 0.153
9.503AsnIle: 9.503 ± 0.666
9.371AsnLys: 9.371 ± 0.615
6.697AsnLeu: 6.697 ± 0.433
1.959AsnMet: 1.959 ± 0.248
7.015AsnAsn: 7.015 ± 0.641
1.853AsnPro: 1.853 ± 0.245
2.303AsnGln: 2.303 ± 0.246
3.203AsnArg: 3.203 ± 0.379
4.924AsnSer: 4.924 ± 0.416
4.129AsnThr: 4.129 ± 0.5
4.024AsnVal: 4.024 ± 0.332
0.662AsnTrp: 0.662 ± 0.125
4.024AsnTyr: 4.024 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
0.715ProAla: 0.715 ± 0.169
0.397ProCys: 0.397 ± 0.114
0.979ProAsp: 0.979 ± 0.16
1.059ProGlu: 1.059 ± 0.179
0.847ProPhe: 0.847 ± 0.148
0.45ProGly: 0.45 ± 0.115
0.397ProHis: 0.397 ± 0.127
2.356ProIle: 2.356 ± 0.205
1.747ProLys: 1.747 ± 0.203
0.979ProLeu: 0.979 ± 0.155
0.265ProMet: 0.265 ± 0.077
1.297ProAsn: 1.297 ± 0.194
0.45ProPro: 0.45 ± 0.128
0.556ProGln: 0.556 ± 0.13
0.476ProArg: 0.476 ± 0.122
1.297ProSer: 1.297 ± 0.169
0.874ProThr: 0.874 ± 0.139
1.138ProVal: 1.138 ± 0.16
0.053ProTrp: 0.053 ± 0.038
0.715ProTyr: 0.715 ± 0.15
0.0ProXaa: 0.0 ± 0.0
Gln
1.218GlnAla: 1.218 ± 0.251
0.344GlnCys: 0.344 ± 0.098
1.853GlnAsp: 1.853 ± 0.262
2.568GlnGlu: 2.568 ± 0.298
1.35GlnPhe: 1.35 ± 0.195
1.271GlnGly: 1.271 ± 0.211
0.529GlnHis: 0.529 ± 0.117
2.435GlnIle: 2.435 ± 0.244
2.674GlnLys: 2.674 ± 0.39
3.415GlnLeu: 3.415 ± 0.49
0.874GlnMet: 0.874 ± 0.191
1.906GlnAsn: 1.906 ± 0.231
0.397GlnPro: 0.397 ± 0.097
1.297GlnGln: 1.297 ± 0.318
1.059GlnArg: 1.059 ± 0.158
1.138GlnSer: 1.138 ± 0.188
0.715GlnThr: 0.715 ± 0.155
1.562GlnVal: 1.562 ± 0.203
0.212GlnTrp: 0.212 ± 0.091
1.085GlnTyr: 1.085 ± 0.167
0.0GlnXaa: 0.0 ± 0.0
Arg
1.403ArgAla: 1.403 ± 0.205
0.582ArgCys: 0.582 ± 0.156
2.197ArgAsp: 2.197 ± 0.295
2.912ArgGlu: 2.912 ± 0.351
1.456ArgPhe: 1.456 ± 0.18
1.403ArgGly: 1.403 ± 0.184
0.397ArgHis: 0.397 ± 0.101
3.732ArgIle: 3.732 ± 0.31
3.971ArgLys: 3.971 ± 0.374
3.494ArgLeu: 3.494 ± 0.338
0.926ArgMet: 0.926 ± 0.173
3.097ArgAsn: 3.097 ± 0.369
0.556ArgPro: 0.556 ± 0.103
1.059ArgGln: 1.059 ± 0.202
0.979ArgArg: 0.979 ± 0.159
1.403ArgSer: 1.403 ± 0.205
1.456ArgThr: 1.456 ± 0.222
2.171ArgVal: 2.171 ± 0.282
0.424ArgTrp: 0.424 ± 0.127
1.509ArgTyr: 1.509 ± 0.206
0.0ArgXaa: 0.0 ± 0.0
Ser
1.8SerAla: 1.8 ± 0.25
0.609SerCys: 0.609 ± 0.151
3.944SerAsp: 3.944 ± 0.279
4.235SerGlu: 4.235 ± 0.372
2.356SerPhe: 2.356 ± 0.21
3.335SerGly: 3.335 ± 0.411
0.556SerHis: 0.556 ± 0.137
6.697SerIle: 6.697 ± 0.438
6.724SerLys: 6.724 ± 0.509
5.241SerLeu: 5.241 ± 0.392
1.694SerMet: 1.694 ± 0.236
4.447SerAsn: 4.447 ± 0.351
0.953SerPro: 0.953 ± 0.181
1.641SerGln: 1.641 ± 0.255
2.171SerArg: 2.171 ± 0.224
4.421SerSer: 4.421 ± 0.354
3.177SerThr: 3.177 ± 0.302
2.806SerVal: 2.806 ± 0.268
0.556SerTrp: 0.556 ± 0.133
2.832SerTyr: 2.832 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
1.932ThrAla: 1.932 ± 0.272
0.847ThrCys: 0.847 ± 0.145
3.468ThrAsp: 3.468 ± 0.316
2.832ThrGlu: 2.832 ± 0.305
2.515ThrPhe: 2.515 ± 0.344
2.727ThrGly: 2.727 ± 0.36
0.582ThrHis: 0.582 ± 0.135
5.162ThrIle: 5.162 ± 0.361
4.977ThrLys: 4.977 ± 0.368
4.129ThrLeu: 4.129 ± 0.446
1.032ThrMet: 1.032 ± 0.171
3.653ThrAsn: 3.653 ± 0.418
1.271ThrPro: 1.271 ± 0.253
1.032ThrGln: 1.032 ± 0.18
1.456ThrArg: 1.456 ± 0.184
3.335ThrSer: 3.335 ± 0.359
2.859ThrThr: 2.859 ± 0.369
2.382ThrVal: 2.382 ± 0.246
0.45ThrTrp: 0.45 ± 0.106
2.488ThrTyr: 2.488 ± 0.244
0.0ThrXaa: 0.0 ± 0.0
Val
2.25ValAla: 2.25 ± 0.257
0.609ValCys: 0.609 ± 0.144
3.6ValAsp: 3.6 ± 0.338
4.129ValGlu: 4.129 ± 0.352
1.985ValPhe: 1.985 ± 0.223
2.938ValGly: 2.938 ± 0.287
0.741ValHis: 0.741 ± 0.149
3.812ValIle: 3.812 ± 0.265
4.897ValLys: 4.897 ± 0.385
3.706ValLeu: 3.706 ± 0.271
1.165ValMet: 1.165 ± 0.169
4.156ValAsn: 4.156 ± 0.398
1.059ValPro: 1.059 ± 0.151
1.429ValGln: 1.429 ± 0.238
1.562ValArg: 1.562 ± 0.241
3.362ValSer: 3.362 ± 0.268
2.594ValThr: 2.594 ± 0.336
2.965ValVal: 2.965 ± 0.304
0.503ValTrp: 0.503 ± 0.107
2.038ValTyr: 2.038 ± 0.226
0.0ValXaa: 0.0 ± 0.0
Trp
0.185TrpAla: 0.185 ± 0.068
0.265TrpCys: 0.265 ± 0.09
0.609TrpAsp: 0.609 ± 0.111
0.582TrpGlu: 0.582 ± 0.136
0.344TrpPhe: 0.344 ± 0.098
0.582TrpGly: 0.582 ± 0.106
0.159TrpHis: 0.159 ± 0.057
0.635TrpIle: 0.635 ± 0.113
0.715TrpLys: 0.715 ± 0.179
0.741TrpLeu: 0.741 ± 0.144
0.159TrpMet: 0.159 ± 0.062
0.847TrpAsn: 0.847 ± 0.143
0.0TrpPro: 0.0 ± 0.0
0.291TrpGln: 0.291 ± 0.097
0.397TrpArg: 0.397 ± 0.116
0.529TrpSer: 0.529 ± 0.107
0.265TrpThr: 0.265 ± 0.074
0.476TrpVal: 0.476 ± 0.148
0.053TrpTrp: 0.053 ± 0.042
0.291TrpTyr: 0.291 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.747TyrAla: 1.747 ± 0.226
0.768TyrCys: 0.768 ± 0.128
3.309TyrAsp: 3.309 ± 0.319
3.494TyrGlu: 3.494 ± 0.296
1.959TyrPhe: 1.959 ± 0.283
2.859TyrGly: 2.859 ± 0.361
0.609TyrHis: 0.609 ± 0.113
5.162TyrIle: 5.162 ± 0.391
5.532TyrLys: 5.532 ± 0.457
4.103TyrLeu: 4.103 ± 0.316
1.324TyrMet: 1.324 ± 0.185
4.288TyrAsn: 4.288 ± 0.36
1.138TyrPro: 1.138 ± 0.216
0.979TyrGln: 0.979 ± 0.173
1.747TyrArg: 1.747 ± 0.289
3.018TyrSer: 3.018 ± 0.293
2.568TyrThr: 2.568 ± 0.261
2.038TyrVal: 2.038 ± 0.278
0.344TyrTrp: 0.344 ± 0.101
2.224TyrTyr: 2.224 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 172 proteins (37778 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski