Sphingomonas phage Eidolon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TC22|A0A6M3TC22_9CAUD Uncharacterized protein OS=Sphingomonas phage Eidolon OX=2686311 PE=4 SV=1
MM1 pKa = 7.73AYY3 pKa = 9.96EE4 pKa = 4.35IDD6 pKa = 3.82TTVLGGLPVTIEE18 pKa = 3.92YY19 pKa = 8.63TVQGAEE25 pKa = 3.92PDD27 pKa = 3.64VGIMSSYY34 pKa = 9.42VDD36 pKa = 3.32EE37 pKa = 4.74WYY39 pKa = 8.59ITAINGRR46 pKa = 11.84PVKK49 pKa = 10.59KK50 pKa = 10.32CDD52 pKa = 2.86WLDD55 pKa = 3.29RR56 pKa = 11.84RR57 pKa = 11.84IAATKK62 pKa = 10.77GEE64 pKa = 4.06TDD66 pKa = 4.32RR67 pKa = 11.84ILEE70 pKa = 4.08EE71 pKa = 4.72LGEE74 pKa = 4.47ADD76 pKa = 4.41HH77 pKa = 7.77DD78 pKa = 5.04DD79 pKa = 4.34YY80 pKa = 12.04YY81 pKa = 11.72DD82 pKa = 4.43DD83 pKa = 4.0YY84 pKa = 12.19

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TC32|A0A6M3TC32_9CAUD Uncharacterized protein OS=Sphingomonas phage Eidolon OX=2686311 PE=4 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84LVVDD7 pKa = 3.76NGSNEE12 pKa = 4.11ATKK15 pKa = 10.55EE16 pKa = 3.35PAMFINHH23 pKa = 6.26NFNVAPVGQNYY34 pKa = 9.69GEE36 pKa = 3.77WSYY39 pKa = 11.96FDD41 pKa = 4.78GYY43 pKa = 11.62AFAHH47 pKa = 5.96IAAWQEE53 pKa = 3.46AARR56 pKa = 11.84LKK58 pKa = 10.49RR59 pKa = 11.84IRR61 pKa = 11.84TTAIKK66 pKa = 10.58ALDD69 pKa = 3.53RR70 pKa = 11.84RR71 pKa = 11.84NVRR74 pKa = 11.84RR75 pKa = 3.64

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13274

31

1090

237.0

25.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.376 ± 0.993

0.889 ± 0.158

6.328 ± 0.278

5.951 ± 0.277

3.827 ± 0.211

8.362 ± 0.395

1.605 ± 0.198

4.934 ± 0.169

5.371 ± 0.389

7.247 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.152

4.641 ± 0.204

4.611 ± 0.35

3.104 ± 0.301

5.326 ± 0.269

5.341 ± 0.19

6.697 ± 0.407

7.089 ± 0.315

1.582 ± 0.161

3.081 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski