Amino acid dipepetide frequency for Sphingomonas phage Eidolon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.767AlaAla: 14.767 ± 2.801
0.678AlaCys: 0.678 ± 0.227
6.253AlaAsp: 6.253 ± 0.737
7.233AlaGlu: 7.233 ± 0.945
3.39AlaPhe: 3.39 ± 0.437
9.041AlaGly: 9.041 ± 0.913
1.959AlaHis: 1.959 ± 0.419
6.479AlaIle: 6.479 ± 0.701
7.007AlaLys: 7.007 ± 1.006
9.116AlaLeu: 9.116 ± 1.156
2.938AlaMet: 2.938 ± 0.489
6.705AlaAsn: 6.705 ± 0.972
3.767AlaPro: 3.767 ± 0.544
3.842AlaGln: 3.842 ± 1.212
5.726AlaArg: 5.726 ± 0.651
5.726AlaSer: 5.726 ± 0.724
7.986AlaThr: 7.986 ± 1.026
6.856AlaVal: 6.856 ± 1.036
1.356AlaTrp: 1.356 ± 0.31
2.938AlaTyr: 2.938 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.678CysAla: 0.678 ± 0.284
0.151CysCys: 0.151 ± 0.102
0.527CysAsp: 0.527 ± 0.195
0.603CysGlu: 0.603 ± 0.237
0.527CysPhe: 0.527 ± 0.224
0.603CysGly: 0.603 ± 0.206
0.527CysHis: 0.527 ± 0.188
0.301CysIle: 0.301 ± 0.132
0.452CysLys: 0.452 ± 0.195
0.603CysLeu: 0.603 ± 0.214
0.075CysMet: 0.075 ± 0.077
0.603CysAsn: 0.603 ± 0.214
0.603CysPro: 0.603 ± 0.19
0.151CysGln: 0.151 ± 0.1
0.603CysArg: 0.603 ± 0.266
0.527CysSer: 0.527 ± 0.143
0.377CysThr: 0.377 ± 0.173
0.452CysVal: 0.452 ± 0.165
0.075CysTrp: 0.075 ± 0.064
0.452CysTyr: 0.452 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
8.137AspAla: 8.137 ± 0.84
0.452AspCys: 0.452 ± 0.172
4.52AspAsp: 4.52 ± 0.881
4.897AspGlu: 4.897 ± 0.53
3.014AspPhe: 3.014 ± 0.402
4.445AspGly: 4.445 ± 0.557
0.678AspHis: 0.678 ± 0.185
2.185AspIle: 2.185 ± 0.376
4.068AspLys: 4.068 ± 0.56
4.37AspLeu: 4.37 ± 0.465
2.26AspMet: 2.26 ± 0.421
2.185AspAsn: 2.185 ± 0.364
3.315AspPro: 3.315 ± 0.458
1.055AspGln: 1.055 ± 0.272
2.938AspArg: 2.938 ± 0.476
2.034AspSer: 2.034 ± 0.365
3.767AspThr: 3.767 ± 0.701
4.897AspVal: 4.897 ± 0.549
1.281AspTrp: 1.281 ± 0.3
2.788AspTyr: 2.788 ± 0.451
0.0AspXaa: 0.0 ± 0.0
Glu
7.082GluAla: 7.082 ± 0.7
0.753GluCys: 0.753 ± 0.295
3.541GluAsp: 3.541 ± 0.451
3.24GluGlu: 3.24 ± 0.589
1.808GluPhe: 1.808 ± 0.418
4.973GluGly: 4.973 ± 0.72
1.13GluHis: 1.13 ± 0.268
3.692GluIle: 3.692 ± 0.605
2.26GluLys: 2.26 ± 0.485
5.199GluLeu: 5.199 ± 0.591
0.979GluMet: 0.979 ± 0.303
1.582GluAsn: 1.582 ± 0.368
2.11GluPro: 2.11 ± 0.482
3.315GluGln: 3.315 ± 0.452
4.37GluArg: 4.37 ± 0.489
1.431GluSer: 1.431 ± 0.38
3.39GluThr: 3.39 ± 0.428
5.199GluVal: 5.199 ± 0.605
0.979GluTrp: 0.979 ± 0.233
2.486GluTyr: 2.486 ± 0.477
0.0GluXaa: 0.0 ± 0.0
Phe
4.37PheAla: 4.37 ± 0.606
0.377PheCys: 0.377 ± 0.159
3.842PheAsp: 3.842 ± 0.456
3.089PheGlu: 3.089 ± 0.494
1.281PhePhe: 1.281 ± 0.29
3.692PheGly: 3.692 ± 0.539
0.527PheHis: 0.527 ± 0.145
2.26PheIle: 2.26 ± 0.313
1.431PheLys: 1.431 ± 0.297
1.582PheLeu: 1.582 ± 0.377
0.678PheMet: 0.678 ± 0.184
1.658PheAsn: 1.658 ± 0.283
2.034PhePro: 2.034 ± 0.405
0.678PheGln: 0.678 ± 0.242
2.26PheArg: 2.26 ± 0.45
2.185PheSer: 2.185 ± 0.363
1.658PheThr: 1.658 ± 0.443
3.014PheVal: 3.014 ± 0.43
0.979PheTrp: 0.979 ± 0.289
0.678PheTyr: 0.678 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
6.856GlyAla: 6.856 ± 0.722
0.527GlyCys: 0.527 ± 0.21
5.726GlyAsp: 5.726 ± 0.62
5.952GlyGlu: 5.952 ± 0.874
3.692GlyPhe: 3.692 ± 0.469
7.835GlyGly: 7.835 ± 1.16
1.733GlyHis: 1.733 ± 0.339
4.37GlyIle: 4.37 ± 0.545
4.52GlyLys: 4.52 ± 0.565
6.027GlyLeu: 6.027 ± 0.52
2.185GlyMet: 2.185 ± 0.356
3.089GlyAsn: 3.089 ± 0.516
2.863GlyPro: 2.863 ± 0.526
3.315GlyGln: 3.315 ± 0.491
4.294GlyArg: 4.294 ± 0.621
4.746GlySer: 4.746 ± 0.581
5.877GlyThr: 5.877 ± 0.717
6.329GlyVal: 6.329 ± 0.676
1.356GlyTrp: 1.356 ± 0.345
2.336GlyTyr: 2.336 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
0.979HisAla: 0.979 ± 0.422
0.075HisCys: 0.075 ± 0.072
0.829HisAsp: 0.829 ± 0.234
0.678HisGlu: 0.678 ± 0.183
0.753HisPhe: 0.753 ± 0.264
1.205HisGly: 1.205 ± 0.322
0.151HisHis: 0.151 ± 0.102
1.055HisIle: 1.055 ± 0.229
0.979HisLys: 0.979 ± 0.272
1.205HisLeu: 1.205 ± 0.355
0.603HisMet: 0.603 ± 0.194
1.055HisAsn: 1.055 ± 0.34
1.205HisPro: 1.205 ± 0.318
0.301HisGln: 0.301 ± 0.128
0.829HisArg: 0.829 ± 0.219
1.431HisSer: 1.431 ± 0.361
0.301HisThr: 0.301 ± 0.136
1.507HisVal: 1.507 ± 0.327
0.452HisTrp: 0.452 ± 0.228
0.452HisTyr: 0.452 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
6.253IleAla: 6.253 ± 0.684
0.753IleCys: 0.753 ± 0.226
3.692IleAsp: 3.692 ± 0.534
3.24IleGlu: 3.24 ± 0.33
1.808IlePhe: 1.808 ± 0.433
3.993IleGly: 3.993 ± 0.559
0.527IleHis: 0.527 ± 0.177
2.411IleIle: 2.411 ± 0.451
2.411IleLys: 2.411 ± 0.439
2.486IleLeu: 2.486 ± 0.457
1.582IleMet: 1.582 ± 0.35
2.712IleAsn: 2.712 ± 0.493
1.733IlePro: 1.733 ± 0.428
1.808IleGln: 1.808 ± 0.381
1.808IleArg: 1.808 ± 0.42
2.712IleSer: 2.712 ± 0.386
3.842IleThr: 3.842 ± 0.407
4.144IleVal: 4.144 ± 0.636
0.226IleTrp: 0.226 ± 0.15
1.205IleTyr: 1.205 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
6.705LysAla: 6.705 ± 0.984
0.377LysCys: 0.377 ± 0.231
2.486LysAsp: 2.486 ± 0.402
2.336LysGlu: 2.336 ± 0.415
2.11LysPhe: 2.11 ± 0.321
3.541LysGly: 3.541 ± 0.499
0.829LysHis: 0.829 ± 0.23
2.26LysIle: 2.26 ± 0.321
3.089LysLys: 3.089 ± 0.521
4.746LysLeu: 4.746 ± 0.718
1.733LysMet: 1.733 ± 0.368
2.185LysAsn: 2.185 ± 0.449
2.938LysPro: 2.938 ± 0.573
1.808LysGln: 1.808 ± 0.361
3.767LysArg: 3.767 ± 0.626
3.014LysSer: 3.014 ± 0.463
3.466LysThr: 3.466 ± 0.52
3.692LysVal: 3.692 ± 0.562
0.678LysTrp: 0.678 ± 0.246
1.884LysTyr: 1.884 ± 0.438
0.0LysXaa: 0.0 ± 0.0
Leu
8.061LeuAla: 8.061 ± 1.155
0.377LeuCys: 0.377 ± 0.146
4.37LeuAsp: 4.37 ± 0.549
3.39LeuGlu: 3.39 ± 0.579
2.788LeuPhe: 2.788 ± 0.534
5.952LeuGly: 5.952 ± 0.682
1.055LeuHis: 1.055 ± 0.284
3.39LeuIle: 3.39 ± 0.499
5.123LeuLys: 5.123 ± 0.816
4.746LeuLeu: 4.746 ± 0.556
1.507LeuMet: 1.507 ± 0.283
2.788LeuAsn: 2.788 ± 0.43
4.144LeuPro: 4.144 ± 0.544
1.959LeuGln: 1.959 ± 0.358
5.048LeuArg: 5.048 ± 0.633
4.445LeuSer: 4.445 ± 0.472
5.048LeuThr: 5.048 ± 0.615
4.596LeuVal: 4.596 ± 0.501
1.281LeuTrp: 1.281 ± 0.389
2.411LeuTyr: 2.411 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
4.144MetAla: 4.144 ± 0.504
0.0MetCys: 0.0 ± 0.0
0.904MetAsp: 0.904 ± 0.231
1.733MetGlu: 1.733 ± 0.318
1.205MetPhe: 1.205 ± 0.327
1.582MetGly: 1.582 ± 0.326
0.151MetHis: 0.151 ± 0.107
0.678MetIle: 0.678 ± 0.222
1.13MetLys: 1.13 ± 0.283
2.336MetLeu: 2.336 ± 0.474
0.979MetMet: 0.979 ± 0.312
1.582MetAsn: 1.582 ± 0.384
1.356MetPro: 1.356 ± 0.341
1.055MetGln: 1.055 ± 0.275
1.13MetArg: 1.13 ± 0.333
1.431MetSer: 1.431 ± 0.329
2.336MetThr: 2.336 ± 0.445
1.808MetVal: 1.808 ± 0.414
0.226MetTrp: 0.226 ± 0.169
0.603MetTyr: 0.603 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
5.199AsnAla: 5.199 ± 0.775
0.226AsnCys: 0.226 ± 0.106
3.315AsnAsp: 3.315 ± 0.575
2.562AsnGlu: 2.562 ± 0.507
1.658AsnPhe: 1.658 ± 0.386
4.746AsnGly: 4.746 ± 0.698
0.904AsnHis: 0.904 ± 0.255
1.959AsnIle: 1.959 ± 0.436
2.486AsnLys: 2.486 ± 0.433
2.562AsnLeu: 2.562 ± 0.387
0.979AsnMet: 0.979 ± 0.238
2.185AsnAsn: 2.185 ± 0.543
2.712AsnPro: 2.712 ± 0.337
1.431AsnGln: 1.431 ± 0.327
2.411AsnArg: 2.411 ± 0.456
2.26AsnSer: 2.26 ± 0.424
2.637AsnThr: 2.637 ± 0.524
4.37AsnVal: 4.37 ± 0.547
0.527AsnTrp: 0.527 ± 0.156
1.281AsnTyr: 1.281 ± 0.241
0.0AsnXaa: 0.0 ± 0.0
Pro
5.425ProAla: 5.425 ± 0.642
0.527ProCys: 0.527 ± 0.182
2.938ProAsp: 2.938 ± 0.524
3.089ProGlu: 3.089 ± 0.467
1.959ProPhe: 1.959 ± 0.319
3.918ProGly: 3.918 ± 0.511
0.678ProHis: 0.678 ± 0.229
2.336ProIle: 2.336 ± 0.49
2.863ProLys: 2.863 ± 0.452
2.788ProLeu: 2.788 ± 0.407
1.055ProMet: 1.055 ± 0.356
2.712ProAsn: 2.712 ± 0.387
2.411ProPro: 2.411 ± 0.509
0.527ProGln: 0.527 ± 0.162
1.733ProArg: 1.733 ± 0.426
2.637ProSer: 2.637 ± 0.411
3.918ProThr: 3.918 ± 0.547
2.863ProVal: 2.863 ± 0.535
0.603ProTrp: 0.603 ± 0.22
1.13ProTyr: 1.13 ± 0.373
0.0ProXaa: 0.0 ± 0.0
Gln
4.144GlnAla: 4.144 ± 0.789
0.452GlnCys: 0.452 ± 0.213
0.904GlnAsp: 0.904 ± 0.262
0.829GlnGlu: 0.829 ± 0.225
1.055GlnPhe: 1.055 ± 0.26
1.959GlnGly: 1.959 ± 0.33
0.753GlnHis: 0.753 ± 0.213
1.507GlnIle: 1.507 ± 0.289
1.13GlnLys: 1.13 ± 0.314
2.562GlnLeu: 2.562 ± 0.383
1.055GlnMet: 1.055 ± 0.226
1.582GlnAsn: 1.582 ± 0.43
1.055GlnPro: 1.055 ± 0.279
1.959GlnGln: 1.959 ± 0.66
2.486GlnArg: 2.486 ± 0.385
2.034GlnSer: 2.034 ± 0.449
1.959GlnThr: 1.959 ± 0.391
1.733GlnVal: 1.733 ± 0.346
0.829GlnTrp: 0.829 ± 0.204
1.055GlnTyr: 1.055 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
5.952ArgAla: 5.952 ± 0.583
0.452ArgCys: 0.452 ± 0.174
3.315ArgAsp: 3.315 ± 0.456
3.24ArgGlu: 3.24 ± 0.379
2.034ArgPhe: 2.034 ± 0.411
4.445ArgGly: 4.445 ± 0.586
1.281ArgHis: 1.281 ± 0.334
3.466ArgIle: 3.466 ± 0.477
2.26ArgLys: 2.26 ± 0.398
3.842ArgLeu: 3.842 ± 0.481
2.11ArgMet: 2.11 ± 0.409
2.411ArgAsn: 2.411 ± 0.403
2.637ArgPro: 2.637 ± 0.466
1.055ArgGln: 1.055 ± 0.299
3.24ArgArg: 3.24 ± 0.564
2.712ArgSer: 2.712 ± 0.536
3.315ArgThr: 3.315 ± 0.458
2.863ArgVal: 2.863 ± 0.403
1.055ArgTrp: 1.055 ± 0.315
1.582ArgTyr: 1.582 ± 0.478
0.0ArgXaa: 0.0 ± 0.0
Ser
5.349SerAla: 5.349 ± 0.703
0.678SerCys: 0.678 ± 0.203
3.39SerAsp: 3.39 ± 0.513
2.26SerGlu: 2.26 ± 0.444
2.411SerPhe: 2.411 ± 0.509
5.575SerGly: 5.575 ± 0.57
0.829SerHis: 0.829 ± 0.299
1.733SerIle: 1.733 ± 0.479
2.411SerLys: 2.411 ± 0.405
4.596SerLeu: 4.596 ± 0.569
1.582SerMet: 1.582 ± 0.375
2.11SerAsn: 2.11 ± 0.35
2.712SerPro: 2.712 ± 0.452
1.658SerGln: 1.658 ± 0.299
2.712SerArg: 2.712 ± 0.366
3.466SerSer: 3.466 ± 0.635
3.466SerThr: 3.466 ± 0.483
3.24SerVal: 3.24 ± 0.566
0.904SerTrp: 0.904 ± 0.247
2.336SerTyr: 2.336 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
7.233ThrAla: 7.233 ± 1.166
0.979ThrCys: 0.979 ± 0.272
4.822ThrAsp: 4.822 ± 0.695
4.219ThrGlu: 4.219 ± 0.669
2.562ThrPhe: 2.562 ± 0.557
6.103ThrGly: 6.103 ± 0.602
0.979ThrHis: 0.979 ± 0.298
3.089ThrIle: 3.089 ± 0.582
3.315ThrLys: 3.315 ± 0.463
5.123ThrLeu: 5.123 ± 0.608
1.507ThrMet: 1.507 ± 0.418
2.637ThrAsn: 2.637 ± 0.495
3.993ThrPro: 3.993 ± 0.525
1.733ThrGln: 1.733 ± 0.352
2.034ThrArg: 2.034 ± 0.375
3.993ThrSer: 3.993 ± 0.523
4.746ThrThr: 4.746 ± 0.74
5.048ThrVal: 5.048 ± 0.985
0.904ThrTrp: 0.904 ± 0.255
1.959ThrTyr: 1.959 ± 0.41
0.0ThrXaa: 0.0 ± 0.0
Val
7.835ValAla: 7.835 ± 0.818
0.603ValCys: 0.603 ± 0.218
4.596ValAsp: 4.596 ± 0.593
4.746ValGlu: 4.746 ± 0.534
2.562ValPhe: 2.562 ± 0.368
5.952ValGly: 5.952 ± 0.587
0.452ValHis: 0.452 ± 0.181
4.445ValIle: 4.445 ± 0.503
4.294ValLys: 4.294 ± 0.684
5.048ValLeu: 5.048 ± 0.498
1.658ValMet: 1.658 ± 0.371
3.315ValAsn: 3.315 ± 0.391
2.863ValPro: 2.863 ± 0.669
1.658ValGln: 1.658 ± 0.332
3.541ValArg: 3.541 ± 0.488
4.219ValSer: 4.219 ± 0.445
5.575ValThr: 5.575 ± 0.848
4.973ValVal: 4.973 ± 0.7
0.904ValTrp: 0.904 ± 0.301
1.658ValTyr: 1.658 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
1.13TrpAla: 1.13 ± 0.314
0.0TrpCys: 0.0 ± 0.0
0.753TrpAsp: 0.753 ± 0.271
0.527TrpGlu: 0.527 ± 0.189
0.904TrpPhe: 0.904 ± 0.25
1.205TrpGly: 1.205 ± 0.395
0.527TrpHis: 0.527 ± 0.198
0.452TrpIle: 0.452 ± 0.178
0.904TrpLys: 0.904 ± 0.298
1.507TrpLeu: 1.507 ± 0.355
0.226TrpMet: 0.226 ± 0.106
1.205TrpAsn: 1.205 ± 0.32
0.527TrpPro: 0.527 ± 0.201
0.753TrpGln: 0.753 ± 0.272
0.829TrpArg: 0.829 ± 0.344
0.904TrpSer: 0.904 ± 0.306
1.733TrpThr: 1.733 ± 0.345
0.678TrpVal: 0.678 ± 0.202
0.301TrpTrp: 0.301 ± 0.162
0.753TrpTyr: 0.753 ± 0.218
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.466TyrAla: 3.466 ± 0.414
0.452TyrCys: 0.452 ± 0.184
2.562TyrAsp: 2.562 ± 0.365
1.658TyrGlu: 1.658 ± 0.483
0.753TyrPhe: 0.753 ± 0.229
2.863TyrGly: 2.863 ± 0.637
0.377TyrHis: 0.377 ± 0.177
1.281TyrIle: 1.281 ± 0.291
1.582TyrLys: 1.582 ± 0.359
2.034TyrLeu: 2.034 ± 0.506
0.603TyrMet: 0.603 ± 0.238
2.11TyrAsn: 2.11 ± 0.485
1.13TyrPro: 1.13 ± 0.309
0.979TyrGln: 0.979 ± 0.166
1.507TyrArg: 1.507 ± 0.368
1.507TyrSer: 1.507 ± 0.34
1.582TyrThr: 1.582 ± 0.306
2.637TyrVal: 2.637 ± 0.376
0.904TyrTrp: 0.904 ± 0.227
0.829TyrTyr: 0.829 ± 0.268
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13274 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski