Thermincola potens (strain JR)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Thermincola; Thermincola potens

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5XDV0|D5XDV0_THEPJ Glycosyl transferase family 2 OS=Thermincola potens (strain JR) OX=635013 GN=TherJR_0955 PE=4 SV=1
MM1 pKa = 6.99SLKK4 pKa = 10.67VAVQKK9 pKa = 10.97GLYY12 pKa = 6.2EE13 pKa = 4.22TEE15 pKa = 4.7RR16 pKa = 11.84VLQDD20 pKa = 2.66SGYY23 pKa = 10.75DD24 pKa = 3.51VVSLEE29 pKa = 4.22EE30 pKa = 4.0NEE32 pKa = 4.48EE33 pKa = 3.97PVDD36 pKa = 4.45AIVYY40 pKa = 10.19SGVSNDD46 pKa = 3.24LTGFDD51 pKa = 3.7SVDD54 pKa = 3.35YY55 pKa = 10.79FGDD58 pKa = 3.72GTTMGINSPSGVLLINAQGYY78 pKa = 9.08SPHH81 pKa = 6.36EE82 pKa = 3.81VAGILNSKK90 pKa = 9.79FSGMGEE96 pKa = 3.88

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5XDV2|D5XDV2_THEPJ Glycosyl transferase family 2 OS=Thermincola potens (strain JR) OX=635013 GN=TherJR_0957 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.47QPKK8 pKa = 8.64NRR10 pKa = 11.84KK11 pKa = 8.1HH12 pKa = 5.86KK13 pKa = 10.04RR14 pKa = 11.84VHH16 pKa = 6.03GFLKK20 pKa = 10.62RR21 pKa = 11.84MSTKK25 pKa = 10.29SGRR28 pKa = 11.84NVLKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.84KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2908

0

2908

885596

30

4140

304.5

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.31 ± 0.044

1.177 ± 0.027

4.948 ± 0.034

6.883 ± 0.052

4.01 ± 0.034

7.687 ± 0.042

1.774 ± 0.027

7.304 ± 0.045

6.646 ± 0.043

9.899 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.021

4.123 ± 0.038

4.184 ± 0.031

3.238 ± 0.027

5.062 ± 0.036

4.969 ± 0.038

5.167 ± 0.055

7.789 ± 0.038

0.949 ± 0.017

3.326 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski