Nannochloropsis gaditana (strain CCMP526) (Green microalga) (Microchloropsis gaditana)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae; Eustigmatales; Monodopsidaceae; Nannochloropsis; Nannochloropsis gaditana

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3328 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K8YTG1|K8YTG1_NANGC Uncharacterized protein (Fragment) OS=Nannochloropsis gaditana (strain CCMP526) OX=1093141 GN=NGA_0221100 PE=4 SV=1
MM1 pKa = 7.56PAGGEE6 pKa = 3.84PLGGNEE12 pKa = 4.75GYY14 pKa = 9.65SQLVHH19 pKa = 6.18YY20 pKa = 9.53PPEE23 pKa = 4.98RR24 pKa = 11.84DD25 pKa = 3.43PPSSLVHH32 pKa = 7.59DD33 pKa = 4.56EE34 pKa = 4.3EE35 pKa = 5.66DD36 pKa = 4.75VGNAWQMPFSPVVDD50 pKa = 4.66LSRR53 pKa = 11.84TPDD56 pKa = 3.26LGEE59 pKa = 4.64DD60 pKa = 3.79EE61 pKa = 5.48EE62 pKa = 4.75EE63 pKa = 4.14EE64 pKa = 4.34CLGEE68 pKa = 4.34EE69 pKa = 4.22GHH71 pKa = 6.53TPQEE75 pKa = 4.04QQ76 pKa = 3.02

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K8YPJ9|K8YPJ9_NANGC Dynein intermediate chain 2 axonemal OS=Nannochloropsis gaditana (strain CCMP526) OX=1093141 GN=DNAI2 PE=4 SV=1
VV1 pKa = 6.44QLLSLAFPPLPGLRR15 pKa = 11.84ARR17 pKa = 11.84SYY19 pKa = 9.22TSPRR23 pKa = 11.84GAPRR27 pKa = 11.84SPRR30 pKa = 11.84PGRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TARR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.47GRR42 pKa = 11.84GRR44 pKa = 11.84RR45 pKa = 11.84PTSPRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84SPRR56 pKa = 11.84GLEE59 pKa = 4.09SPGPARR65 pKa = 11.84PGPLGPPP72 pKa = 3.54

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3328

0

3328

996625

8

4759

299.5

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.557 ± 0.045

1.531 ± 0.019

4.791 ± 0.034

6.941 ± 0.055

3.562 ± 0.029

8.512 ± 0.056

2.412 ± 0.02

3.524 ± 0.029

4.454 ± 0.043

9.951 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.02

2.583 ± 0.026

5.959 ± 0.052

3.658 ± 0.031

6.986 ± 0.044

7.619 ± 0.053

5.118 ± 0.034

6.891 ± 0.037

1.302 ± 0.017

2.35 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski