Amino acid dipepetide frequency for Nannochloropsis gaditana (strain CCMP526) (Green microalga) (Microchloropsis gaditana)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.924AlaAla: 10.924 ± 0.16	1.761AlaCys: 1.761 ± 0.042	3.807AlaAsp: 3.807 ± 0.066	5.492AlaGlu: 5.492 ± 0.089	3.819AlaPhe: 3.819 ± 0.066	7.728AlaGly: 7.728 ± 0.094	2.022AlaHis: 2.022 ± 0.045	3.306AlaIle: 3.306 ± 0.069	3.732AlaLys: 3.732 ± 0.072	10.458AlaLeu: 10.458 ± 0.114	2.336AlaMet: 2.336 ± 0.054	2.034AlaAsn: 2.034 ± 0.053	5.191AlaPro: 5.191 ± 0.092	3.263AlaGln: 3.263 ± 0.069	6.524AlaArg: 6.524 ± 0.094	8.039AlaSer: 8.039 ± 0.104	4.981AlaThr: 4.981 ± 0.079	6.57AlaVal: 6.57 ± 0.093	1.384AlaTrp: 1.384 ± 0.042	2.197AlaTyr: 2.197 ± 0.053	0.005AlaXaa: 0.005 ± 0.002
Cys	1.162CysAla: 1.162 ± 0.034	0.342CysCys: 0.342 ± 0.021	0.66CysAsp: 0.66 ± 0.025	0.747CysGlu: 0.747 ± 0.028	0.653CysPhe: 0.653 ± 0.025	1.263CysGly: 1.263 ± 0.031	0.38CysHis: 0.38 ± 0.019	0.633CysIle: 0.633 ± 0.026	0.599CysLys: 0.599 ± 0.026	1.874CysLeu: 1.874 ± 0.051	0.333CysMet: 0.333 ± 0.02	0.44CysAsn: 0.44 ± 0.019	1.063CysPro: 1.063 ± 0.042	0.587CysGln: 0.587 ± 0.023	1.017CysArg: 1.017 ± 0.034	1.094CysSer: 1.094 ± 0.035	0.805CysThr: 0.805 ± 0.026	1.08CysVal: 1.08 ± 0.031	0.204CysTrp: 0.204 ± 0.014	0.376CysTyr: 0.376 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	4.654AspAla: 4.654 ± 0.07	0.69AspCys: 0.69 ± 0.027	2.743AspAsp: 2.743 ± 0.063	3.675AspGlu: 3.675 ± 0.081	1.867AspPhe: 1.867 ± 0.049	4.475AspGly: 4.475 ± 0.078	0.971AspHis: 0.971 ± 0.033	1.877AspIle: 1.877 ± 0.047	2.065AspLys: 2.065 ± 0.046	4.765AspLeu: 4.765 ± 0.067	1.24AspMet: 1.24 ± 0.04	1.097AspAsn: 1.097 ± 0.035	2.825AspPro: 2.825 ± 0.058	1.349AspGln: 1.349 ± 0.037	2.85AspArg: 2.85 ± 0.052	2.706AspSer: 2.706 ± 0.056	2.223AspThr: 2.223 ± 0.047	3.922AspVal: 3.922 ± 0.067	0.69AspTrp: 0.69 ± 0.032	1.224AspTyr: 1.224 ± 0.035	0.005AspXaa: 0.005 ± 0.002
Glu	7.245GluAla: 7.245 ± 0.1	0.782GluCys: 0.782 ± 0.027	4.399GluAsp: 4.399 ± 0.084	9.494GluGlu: 9.494 ± 0.24	1.396GluPhe: 1.396 ± 0.036	7.633GluGly: 7.633 ± 0.128	1.319GluHis: 1.319 ± 0.037	2.318GluIle: 2.318 ± 0.053	3.978GluLys: 3.978 ± 0.087	4.903GluLeu: 4.903 ± 0.077	1.683GluMet: 1.683 ± 0.04	1.825GluAsn: 1.825 ± 0.04	2.122GluPro: 2.122 ± 0.049	2.21GluGln: 2.21 ± 0.047	4.871GluArg: 4.871 ± 0.087	3.539GluSer: 3.539 ± 0.058	3.061GluThr: 3.061 ± 0.047	4.483GluVal: 4.483 ± 0.084	0.829GluTrp: 0.829 ± 0.034	1.316GluTyr: 1.316 ± 0.035	0.004GluXaa: 0.004 ± 0.002
Phe	2.927PheAla: 2.927 ± 0.062	0.619PheCys: 0.619 ± 0.026	1.799PheAsp: 1.799 ± 0.041	1.826PheGlu: 1.826 ± 0.043	1.712PhePhe: 1.712 ± 0.048	2.483PheGly: 2.483 ± 0.051	0.999PheHis: 0.999 ± 0.032	1.327PheIle: 1.327 ± 0.037	1.317PheLys: 1.317 ± 0.035	4.415PheLeu: 4.415 ± 0.066	0.731PheMet: 0.731 ± 0.027	1.012PheAsn: 1.012 ± 0.034	2.229PhePro: 2.229 ± 0.049	1.337PheGln: 1.337 ± 0.038	2.131PheArg: 2.131 ± 0.042	2.875PheSer: 2.875 ± 0.058	1.748PheThr: 1.748 ± 0.04	2.564PheVal: 2.564 ± 0.053	0.508PheTrp: 0.508 ± 0.024	1.062PheTyr: 1.062 ± 0.034	0.001PheXaa: 0.001 ± 0.001
Gly	7.199GlyAla: 7.199 ± 0.104	1.152GlyCys: 1.152 ± 0.039	3.848GlyAsp: 3.848 ± 0.07	6.055GlyGlu: 6.055 ± 0.091	2.648GlyPhe: 2.648 ± 0.056	10.601GlyGly: 10.601 ± 0.185	1.937GlyHis: 1.937 ± 0.048	2.94GlyIle: 2.94 ± 0.06	4.776GlyLys: 4.776 ± 0.071	7.265GlyLeu: 7.265 ± 0.096	2.159GlyMet: 2.159 ± 0.045	2.437GlyAsn: 2.437 ± 0.058	3.863GlyPro: 3.863 ± 0.069	2.972GlyGln: 2.972 ± 0.064	6.919GlyArg: 6.919 ± 0.107	5.645GlySer: 5.645 ± 0.08	4.033GlyThr: 4.033 ± 0.067	5.716GlyVal: 5.716 ± 0.082	1.137GlyTrp: 1.137 ± 0.035	1.795GlyTyr: 1.795 ± 0.044	0.019GlyXaa: 0.019 ± 0.004
His	2.329HisAla: 2.329 ± 0.048	0.42HisCys: 0.42 ± 0.021	0.974HisAsp: 0.974 ± 0.034	1.252HisGlu: 1.252 ± 0.034	0.9HisPhe: 0.9 ± 0.03	1.999HisGly: 1.999 ± 0.05	0.884HisHis: 0.884 ± 0.036	0.86HisIle: 0.86 ± 0.033	0.828HisLys: 0.828 ± 0.031	2.445HisLeu: 2.445 ± 0.056	0.521HisMet: 0.521 ± 0.025	0.58HisAsn: 0.58 ± 0.025	1.753HisPro: 1.753 ± 0.04	0.991HisGln: 0.991 ± 0.038	1.81HisArg: 1.81 ± 0.046	1.568HisSer: 1.568 ± 0.041	1.104HisThr: 1.104 ± 0.029	1.949HisVal: 1.949 ± 0.045	0.3HisTrp: 0.3 ± 0.019	0.655HisTyr: 0.655 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	3.072IleAla: 3.072 ± 0.055	0.639IleCys: 0.639 ± 0.028	1.853IleAsp: 1.853 ± 0.045	2.042IleGlu: 2.042 ± 0.042	1.565IlePhe: 1.565 ± 0.043	2.424IleGly: 2.424 ± 0.058	0.828IleHis: 0.828 ± 0.027	1.426IleIle: 1.426 ± 0.041	1.69IleLys: 1.69 ± 0.041	3.928IleLeu: 3.928 ± 0.074	0.871IleMet: 0.871 ± 0.032	1.089IleAsn: 1.089 ± 0.036	2.097IlePro: 2.097 ± 0.043	1.349IleGln: 1.349 ± 0.04	2.214IleArg: 2.214 ± 0.046	2.508IleSer: 2.508 ± 0.051	1.809IleThr: 1.809 ± 0.044	2.37IleVal: 2.37 ± 0.053	0.486IleTrp: 0.486 ± 0.023	0.979IleTyr: 0.979 ± 0.032	0.001IleXaa: 0.001 ± 0.001
Lys	4.606LysAla: 4.606 ± 0.076	0.544LysCys: 0.544 ± 0.025	2.393LysAsp: 2.393 ± 0.052	4.303LysGlu: 4.303 ± 0.086	1.097LysPhe: 1.097 ± 0.035	4.008LysGly: 4.008 ± 0.062	1.017LysHis: 1.017 ± 0.035	1.603LysIle: 1.603 ± 0.038	3.128LysLys: 3.128 ± 0.075	3.335LysLeu: 3.335 ± 0.069	1.053LysMet: 1.053 ± 0.035	1.261LysAsn: 1.261 ± 0.039	1.939LysPro: 1.939 ± 0.046	1.565LysGln: 1.565 ± 0.044	3.42LysArg: 3.42 ± 0.07	2.534LysSer: 2.534 ± 0.051	2.252LysThr: 2.252 ± 0.043	2.97LysVal: 2.97 ± 0.054	0.493LysTrp: 0.493 ± 0.026	1.018LysTyr: 1.018 ± 0.036	0.002LysXaa: 0.002 ± 0.001
Leu	9.576LeuAla: 9.576 ± 0.1	1.655LeuCys: 1.655 ± 0.046	4.747LeuAsp: 4.747 ± 0.071	6.059LeuGlu: 6.059 ± 0.102	3.637LeuPhe: 3.637 ± 0.074	7.013LeuGly: 7.013 ± 0.1	2.592LeuHis: 2.592 ± 0.062	3.158LeuIle: 3.158 ± 0.058	4.035LeuLys: 4.035 ± 0.072	11.241LeuLeu: 11.241 ± 0.166	1.971LeuMet: 1.971 ± 0.048	2.526LeuAsn: 2.526 ± 0.056	7.098LeuPro: 7.098 ± 0.104	4.03LeuGln: 4.03 ± 0.076	7.088LeuArg: 7.088 ± 0.087	7.935LeuSer: 7.935 ± 0.107	4.821LeuThr: 4.821 ± 0.081	6.787LeuVal: 6.787 ± 0.103	1.212LeuTrp: 1.212 ± 0.035	2.329LeuTyr: 2.329 ± 0.054	0.007LeuXaa: 0.007 ± 0.002
Met	2.521MetAla: 2.521 ± 0.058	0.288MetCys: 0.288 ± 0.018	1.34MetAsp: 1.34 ± 0.035	1.867MetGlu: 1.867 ± 0.042	0.598MetPhe: 0.598 ± 0.023	1.908MetGly: 1.908 ± 0.049	0.53MetHis: 0.53 ± 0.022	0.72MetIle: 0.72 ± 0.028	1.011MetLys: 1.011 ± 0.033	2.038MetLeu: 2.038 ± 0.056	0.544MetMet: 0.544 ± 0.023	0.621MetAsn: 0.621 ± 0.026	1.163MetPro: 1.163 ± 0.034	0.885MetGln: 0.885 ± 0.03	1.493MetArg: 1.493 ± 0.039	1.571MetSer: 1.571 ± 0.042	1.257MetThr: 1.257 ± 0.038	1.601MetVal: 1.601 ± 0.038	0.245MetTrp: 0.245 ± 0.017	0.463MetTyr: 0.463 ± 0.021	0.001MetXaa: 0.001 ± 0.001
Asn	2.415AsnAla: 2.415 ± 0.058	0.331AsnCys: 0.331 ± 0.019	1.181AsnAsp: 1.181 ± 0.032	1.558AsnGlu: 1.558 ± 0.036	1.079AsnPhe: 1.079 ± 0.034	2.188AsnGly: 2.188 ± 0.054	0.611AsnHis: 0.611 ± 0.023	1.091AsnIle: 1.091 ± 0.036	1.226AsnLys: 1.226 ± 0.033	2.574AsnLeu: 2.574 ± 0.05	0.61AsnMet: 0.61 ± 0.025	0.819AsnAsn: 0.819 ± 0.05	1.705AsnPro: 1.705 ± 0.042	0.868AsnGln: 0.868 ± 0.047	1.569AsnArg: 1.569 ± 0.039	1.587AsnSer: 1.587 ± 0.048	1.407AsnThr: 1.407 ± 0.036	2.053AsnVal: 2.053 ± 0.048	0.322AsnTrp: 0.322 ± 0.019	0.636AsnTyr: 0.636 ± 0.023	0.001AsnXaa: 0.001 ± 0.001
Pro	5.453ProAla: 5.453 ± 0.085	0.879ProCys: 0.879 ± 0.033	2.437ProAsp: 2.437 ± 0.057	3.134ProGlu: 3.134 ± 0.056	2.341ProPhe: 2.341 ± 0.05	4.302ProGly: 4.302 ± 0.082	1.496ProHis: 1.496 ± 0.035	1.698ProIle: 1.698 ± 0.044	1.769ProLys: 1.769 ± 0.049	5.678ProLeu: 5.678 ± 0.069	1.018ProMet: 1.018 ± 0.031	1.282ProAsn: 1.282 ± 0.039	6.36ProPro: 6.36 ± 0.155	1.955ProGln: 1.955 ± 0.054	3.659ProArg: 3.659 ± 0.066	6.953ProSer: 6.953 ± 0.122	3.371ProThr: 3.371 ± 0.069	3.811ProVal: 3.811 ± 0.072	0.713ProTrp: 0.713 ± 0.027	1.27ProTyr: 1.27 ± 0.035	0.007ProXaa: 0.007 ± 0.003
Gln	4.185GlnAla: 4.185 ± 0.072	0.533GlnCys: 0.533 ± 0.024	1.858GlnAsp: 1.858 ± 0.044	2.968GlnGlu: 2.968 ± 0.06	0.871GlnPhe: 0.871 ± 0.033	3.28GlnGly: 3.28 ± 0.069	1.067GlnHis: 1.067 ± 0.034	1.225GlnIle: 1.225 ± 0.033	1.548GlnLys: 1.548 ± 0.043	2.943GlnLeu: 2.943 ± 0.056	0.687GlnMet: 0.687 ± 0.027	0.932GlnAsn: 0.932 ± 0.034	1.794GlnPro: 1.794 ± 0.054	2.37GlnGln: 2.37 ± 0.119	2.649GlnArg: 2.649 ± 0.061	2.166GlnSer: 2.166 ± 0.051	1.685GlnThr: 1.685 ± 0.041	2.499GlnVal: 2.499 ± 0.056	0.454GlnTrp: 0.454 ± 0.026	0.868GlnTyr: 0.868 ± 0.034	0.001GlnXaa: 0.001 ± 0.001
Arg	6.163ArgAla: 6.163 ± 0.085	0.932ArgCys: 0.932 ± 0.036	3.208ArgAsp: 3.208 ± 0.065	5.431ArgGlu: 5.431 ± 0.081	2.297ArgPhe: 2.297 ± 0.05	5.755ArgGly: 5.755 ± 0.098	1.852ArgHis: 1.852 ± 0.044	2.395ArgIle: 2.395 ± 0.057	3.478ArgLys: 3.478 ± 0.072	6.869ArgLeu: 6.869 ± 0.081	1.639ArgMet: 1.639 ± 0.043	1.733ArgAsn: 1.733 ± 0.043	3.881ArgPro: 3.881 ± 0.067	2.849ArgGln: 2.849 ± 0.06	6.604ArgArg: 6.604 ± 0.102	4.403ArgSer: 4.403 ± 0.07	3.205ArgThr: 3.205 ± 0.057	4.646ArgVal: 4.646 ± 0.073	0.906ArgTrp: 0.906 ± 0.032	1.607ArgTyr: 1.607 ± 0.039	0.004ArgXaa: 0.004 ± 0.002
Ser	6.233SerAla: 6.233 ± 0.087	1.139SerCys: 1.139 ± 0.038	2.861SerAsp: 2.861 ± 0.061	3.497SerGlu: 3.497 ± 0.061	3.134SerPhe: 3.134 ± 0.066	5.588SerGly: 5.588 ± 0.083	1.782SerHis: 1.782 ± 0.04	2.853SerIle: 2.853 ± 0.058	2.951SerLys: 2.951 ± 0.05	8.236SerLeu: 8.236 ± 0.097	1.653SerMet: 1.653 ± 0.04	1.996SerAsn: 1.996 ± 0.051	5.495SerPro: 5.495 ± 0.109	2.415SerGln: 2.415 ± 0.05	4.87SerArg: 4.87 ± 0.081	9.654SerSer: 9.654 ± 0.185	4.729SerThr: 4.729 ± 0.083	4.538SerVal: 4.538 ± 0.069	0.925SerTrp: 0.925 ± 0.032	1.638SerTyr: 1.638 ± 0.04	0.001SerXaa: 0.001 ± 0.001
Thr	5.195ThrAla: 5.195 ± 0.078	0.861ThrCys: 0.861 ± 0.034	2.192ThrAsp: 2.192 ± 0.052	2.824ThrGlu: 2.824 ± 0.058	1.99ThrPhe: 1.99 ± 0.041	4.404ThrGly: 4.404 ± 0.071	1.148ThrHis: 1.148 ± 0.03	1.889ThrIle: 1.889 ± 0.044	1.951ThrLys: 1.951 ± 0.045	5.262ThrLeu: 5.262 ± 0.072	1.087ThrMet: 1.087 ± 0.038	1.279ThrAsn: 1.279 ± 0.036	3.404ThrPro: 3.404 ± 0.062	1.655ThrGln: 1.655 ± 0.046	3.13ThrArg: 3.13 ± 0.052	4.461ThrSer: 4.461 ± 0.079	3.019ThrThr: 3.019 ± 0.074	3.441ThrVal: 3.441 ± 0.06	0.689ThrTrp: 0.689 ± 0.028	1.297ThrTyr: 1.297 ± 0.041	0.003ThrXaa: 0.003 ± 0.002
Val	6.519ValAla: 6.519 ± 0.089	1.099ValCys: 1.099 ± 0.034	3.654ValAsp: 3.654 ± 0.064	4.788ValGlu: 4.788 ± 0.071	2.585ValPhe: 2.585 ± 0.055	5.334ValGly: 5.334 ± 0.09	1.692ValHis: 1.692 ± 0.041	2.457ValIle: 2.457 ± 0.051	2.867ValLys: 2.867 ± 0.059	7.519ValLeu: 7.519 ± 0.099	1.627ValMet: 1.627 ± 0.044	1.754ValAsn: 1.754 ± 0.047	3.775ValPro: 3.775 ± 0.073	2.469ValGln: 2.469 ± 0.054	4.447ValArg: 4.447 ± 0.067	4.641ValSer: 4.641 ± 0.064	3.599ValThr: 3.599 ± 0.056	5.436ValVal: 5.436 ± 0.075	0.946ValTrp: 0.946 ± 0.031	1.691ValTyr: 1.691 ± 0.047	0.007ValXaa: 0.007 ± 0.003
Trp	1.17TrpAla: 1.17 ± 0.035	0.217TrpCys: 0.217 ± 0.013	0.695TrpAsp: 0.695 ± 0.03	1.02TrpGlu: 1.02 ± 0.038	0.388TrpPhe: 0.388 ± 0.02	0.98TrpGly: 0.98 ± 0.03	0.338TrpHis: 0.338 ± 0.018	0.526TrpIle: 0.526 ± 0.025	0.663TrpLys: 0.663 ± 0.025	1.185TrpLeu: 1.185 ± 0.038	0.328TrpMet: 0.328 ± 0.02	0.412TrpAsn: 0.412 ± 0.035	0.579TrpPro: 0.579 ± 0.026	0.517TrpGln: 0.517 ± 0.025	1.007TrpArg: 1.007 ± 0.034	0.836TrpSer: 0.836 ± 0.029	0.777TrpThr: 0.777 ± 0.037	0.866TrpVal: 0.866 ± 0.033	0.22TrpTrp: 0.22 ± 0.015	0.291TrpTyr: 0.291 ± 0.016	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.016TyrAla: 2.016 ± 0.045	0.429TyrCys: 0.429 ± 0.022	1.26TyrAsp: 1.26 ± 0.035	1.377TyrGlu: 1.377 ± 0.043	1.046TyrPhe: 1.046 ± 0.033	1.743TyrGly: 1.743 ± 0.044	0.657TyrHis: 0.657 ± 0.024	0.938TyrIle: 0.938 ± 0.032	0.923TyrLys: 0.923 ± 0.029	2.579TyrLeu: 2.579 ± 0.059	0.575TyrMet: 0.575 ± 0.023	0.699TyrAsn: 0.699 ± 0.027	1.244TyrPro: 1.244 ± 0.037	0.943TyrGln: 0.943 ± 0.031	1.574TyrArg: 1.574 ± 0.039	1.465TyrSer: 1.465 ± 0.037	1.292TyrThr: 1.292 ± 0.037	1.6TyrVal: 1.6 ± 0.044	0.353TyrTrp: 0.353 ± 0.02	0.78TyrTyr: 0.78 ± 0.029	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.008XaaAla: 0.008 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.002XaaAsp: 0.002 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.007XaaGly: 0.007 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.004XaaLys: 0.004 ± 0.002	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.011XaaPro: 0.011 ± 0.003	0.006XaaGln: 0.006 ± 0.002	0.008XaaArg: 0.008 ± 0.003	0.005XaaSer: 0.005 ± 0.002	0.003XaaThr: 0.003 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.002XaaTrp: 0.002 ± 0.001	0.003XaaTyr: 0.003 ± 0.002	0.253XaaXaa: 0.253 ± 0.04

Statistics based on 3328 proteins (996625 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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