Brenneria goodwinii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Brenneria

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G4K130|A0A0G4K130_9GAMM Protease subunit of ATP-dependent Clp proteases OS=Brenneria goodwinii OX=1109412 GN=BN1221_04116c PE=4 SV=1
MM1 pKa = 7.54AVYY4 pKa = 10.15KK5 pKa = 10.69IKK7 pKa = 10.9DD8 pKa = 3.39LTTNSEE14 pKa = 3.75FDD16 pKa = 3.79APDD19 pKa = 3.31DD20 pKa = 4.33VYY22 pKa = 11.3ILDD25 pKa = 3.69SFEE28 pKa = 4.11NSGIEE33 pKa = 4.14LPYY36 pKa = 10.04SCRR39 pKa = 11.84AGACSTCVALLISGQVDD56 pKa = 3.38QSDD59 pKa = 3.7GSFLLEE65 pKa = 3.69EE66 pKa = 4.39QKK68 pKa = 10.89NHH70 pKa = 7.7FILTCVAYY78 pKa = 9.76PMSDD82 pKa = 3.35CVIKK86 pKa = 10.08TGVEE90 pKa = 3.79SALYY94 pKa = 10.66DD95 pKa = 3.83DD96 pKa = 4.31MQNRR100 pKa = 11.84DD101 pKa = 3.7LFEE104 pKa = 5.41DD105 pKa = 3.55ALEE108 pKa = 4.21ALFDD112 pKa = 4.07SFDD115 pKa = 3.98GSDD118 pKa = 3.78SEE120 pKa = 4.39QLASGLLAPIDD131 pKa = 3.8AFRR134 pKa = 11.84HH135 pKa = 4.88YY136 pKa = 11.06LFGDD140 pKa = 3.78GSDD143 pKa = 3.38RR144 pKa = 11.84YY145 pKa = 11.41VNIDD149 pKa = 4.17DD150 pKa = 3.93IGLNIEE156 pKa = 4.14INQIPSVMDD165 pKa = 4.26IINSNAIGSFDD176 pKa = 3.72ISNDD180 pKa = 3.15FNRR183 pKa = 11.84NTSLDD188 pKa = 3.47GFIPAAYY195 pKa = 9.85LGNITMKK202 pKa = 10.67TEE204 pKa = 3.75GTLNISPDD212 pKa = 3.42GSWNYY217 pKa = 10.68NGVIRR222 pKa = 11.84AYY224 pKa = 10.79NDD226 pKa = 3.48RR227 pKa = 11.84YY228 pKa = 10.47DD229 pKa = 4.62ANPSTHH235 pKa = 6.98RR236 pKa = 11.84DD237 pKa = 3.45ALGEE241 pKa = 3.92WSTSILEE248 pKa = 4.4TLSGTPYY255 pKa = 10.48DD256 pKa = 3.42ISIPGEE262 pKa = 3.98LEE264 pKa = 3.27ISGNGKK270 pKa = 9.45RR271 pKa = 4.14

Molecular weight: 29.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G4JU06|A0A0G4JU06_9GAMM Acetylglutamate kinase OS=Brenneria goodwinii OX=1109412 GN=argB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4958

0

4958

1522365

37

8524

307.1

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.848 ± 0.054

1.051 ± 0.014

5.262 ± 0.032

5.396 ± 0.035

3.8 ± 0.037

7.392 ± 0.037

2.202 ± 0.018

6.139 ± 0.034

3.942 ± 0.042

10.985 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.026

3.771 ± 0.041

4.5 ± 0.027

4.617 ± 0.04

5.858 ± 0.05

6.203 ± 0.039

5.273 ± 0.081

6.848 ± 0.053

1.379 ± 0.015

2.892 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski