Axonopus compressus streak virus
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2F2A2|X2F2A2_9GEMI Replication-associated protein OS=Axonopus compressus streak virus OX=1476487 PE=3 SV=1
MM1 pKa = 7.53 NNTEE5 pKa = 4.28 HH6 pKa = 6.94 GGPSGFRR13 pKa = 11.84 FQSRR17 pKa = 11.84 NIFLTYY23 pKa = 9.41 PRR25 pKa = 11.84 CNLAPEE31 pKa = 5.36 LIGSFLLSLLSPYY44 pKa = 10.2 HH45 pKa = 7.53 VMFITVTSEE54 pKa = 3.51 LHH56 pKa = 6.87 KK57 pKa = 11.09 DD58 pKa = 3.67 GTPHH62 pKa = 5.92 IHH64 pKa = 7.36 ALAQTDD70 pKa = 3.9 KK71 pKa = 10.75 RR72 pKa = 11.84 VHH74 pKa = 5.94 TYY76 pKa = 10.51 SPGFFDD82 pKa = 4.15 VQGFHH87 pKa = 7.37 PNIQSARR94 pKa = 11.84 SPQTVLSYY102 pKa = 10.64 ILKK105 pKa = 10.49 SPTGTFNYY113 pKa = 10.34 GSLRR117 pKa = 11.84 PRR119 pKa = 11.84 GTRR122 pKa = 11.84 ADD124 pKa = 3.67 AACGIGEE131 pKa = 4.13 DD132 pKa = 4.03 TAGGGASSSSSPPQADD148 pKa = 3.12 QRR150 pKa = 11.84 RR151 pKa = 11.84 GSRR154 pKa = 11.84 SRR156 pKa = 11.84 EE157 pKa = 3.81 LANDD161 pKa = 3.99 PGRR164 pKa = 11.84 DD165 pKa = 3.32 RR166 pKa = 11.84 KK167 pKa = 10.58 DD168 pKa = 3.38 VLMTSILAGSSSKK181 pKa = 10.78 QEE183 pKa = 3.91 FLNGVKK189 pKa = 10.2 KK190 pKa = 10.69 AFPYY194 pKa = 10.28 DD195 pKa = 3.73 FCARR199 pKa = 11.84 LQNWEE204 pKa = 3.82 YY205 pKa = 11.16 AANKK209 pKa = 10.22 LFDD212 pKa = 3.92 TPAVYY217 pKa = 10.06 QPPFPDD223 pKa = 3.91 SYY225 pKa = 10.43 FHH227 pKa = 6.21 CHH229 pKa = 5.63 EE230 pKa = 5.58 NIHH233 pKa = 6.56 DD234 pKa = 3.85 WVRR237 pKa = 11.84 DD238 pKa = 3.74 NIYY241 pKa = 10.35 EE242 pKa = 4.07 ITPEE246 pKa = 4.32 VYY248 pKa = 10.54 SLLHH252 pKa = 6.7 PYY254 pKa = 11.11 ANAQEE259 pKa = 4.18 DD260 pKa = 4.59 LQWLHH265 pKa = 5.15 NTVMNRR271 pKa = 11.84 QDD273 pKa = 3.88 PSPSTSVDD281 pKa = 3.15 QQGQANQHH289 pKa = 5.8 GPEE292 pKa = 4.08 AWDD295 pKa = 3.42 DD296 pKa = 3.79 TTTGKK301 pKa = 10.33 IMM303 pKa = 4.26
Molecular weight: 33.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.89
IPC2_protein 5.817
IPC_protein 5.906
Toseland 6.402
ProMoST 6.287
Dawson 6.224
Bjellqvist 6.211
Wikipedia 6.262
Rodwell 6.224
Grimsley 6.59
Solomon 6.237
Lehninger 6.224
Nozaki 6.517
DTASelect 6.707
Thurlkill 6.737
EMBOSS 6.707
Sillero 6.62
Patrickios 3.923
IPC_peptide 6.249
IPC2_peptide 6.62
IPC2.peptide.svr19 6.519
Protein with the highest isoelectric point:
>tr|X2EZP9|X2EZP9_9GEMI Capsid protein OS=Axonopus compressus streak virus OX=1476487 PE=3 SV=1
MM1 pKa = 7.75 FYY3 pKa = 10.76 RR4 pKa = 11.84 MEE6 pKa = 5.12 RR7 pKa = 11.84 GPLPQVSSVYY17 pKa = 10.53 VLSPPAGPAPTTFVGNDD34 pKa = 3.68 GAWRR38 pKa = 11.84 FLVLLLACTALAGGTLFACWRR59 pKa = 11.84 LLGKK63 pKa = 10.15 DD64 pKa = 3.65 LLLTLKK70 pKa = 10.3 ARR72 pKa = 11.84 RR73 pKa = 11.84 SRR75 pKa = 11.84 TVTEE79 pKa = 4.46 LGFGQTPPGRR89 pKa = 11.84 QAGAGSQPVGPFGG102 pKa = 3.99
Molecular weight: 10.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.619
IPC_protein 10.57
Toseland 10.467
ProMoST 10.277
Dawson 10.628
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.701
Grimsley 10.701
Solomon 10.76
Lehninger 10.716
Nozaki 10.526
DTASelect 10.394
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.54
IPC_peptide 10.76
IPC2_peptide 9.75
IPC2.peptide.svr19 8.385
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1024
102
382
256.0
28.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.836 ± 0.439
2.344 ± 0.496
5.957 ± 0.511
3.418 ± 0.464
4.785 ± 0.407
8.008 ± 0.727
2.93 ± 0.553
4.004 ± 0.691
3.906 ± 0.549
7.52 ± 0.941
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.465 ± 0.114
4.492 ± 0.489
6.836 ± 0.633
3.906 ± 0.686
6.934 ± 0.854
8.301 ± 0.609
6.836 ± 0.545
5.664 ± 0.905
1.855 ± 0.333
4.004 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here