Streptococcus satellite phage Javan67
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZYJ8|A0A4D5ZYJ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan67 OX=2558810 GN=JavanS67_0005 PE=4 SV=1
MM1 pKa = 7.77 ADD3 pKa = 3.37 KK4 pKa = 11.27 KK5 pKa = 11.09 EE6 pKa = 3.65 FDD8 pKa = 4.13 LANEE12 pKa = 3.91 RR13 pKa = 11.84 AKK15 pKa = 11.2 NFGIWLEE22 pKa = 4.04 EE23 pKa = 4.33 AYY25 pKa = 8.85 QTMLDD30 pKa = 4.45 FSLEE34 pKa = 4.24 DD35 pKa = 4.04 KK36 pKa = 10.56 FDD38 pKa = 4.03 CYY40 pKa = 11.03 SIEE43 pKa = 4.08 EE44 pKa = 4.21 RR45 pKa = 11.84 NQLEE49 pKa = 4.34 RR50 pKa = 11.84 VLEE53 pKa = 4.07 TLMDD57 pKa = 4.18 FCDD60 pKa = 3.07 MWEE63 pKa = 4.02 RR64 pKa = 11.84 GQIILASKK72 pKa = 9.62 EE73 pKa = 3.85 RR74 pKa = 11.84 EE75 pKa = 4.13 AIEE78 pKa = 3.87
Molecular weight: 9.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.473
IPC2_protein 4.342
IPC_protein 4.228
Toseland 4.075
ProMoST 4.279
Dawson 4.177
Bjellqvist 4.38
Wikipedia 4.037
Rodwell 4.062
Grimsley 3.986
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.406
Thurlkill 4.075
EMBOSS 4.05
Sillero 4.329
Patrickios 3.897
IPC_peptide 4.164
IPC2_peptide 4.329
IPC2.peptide.svr19 4.251
Protein with the highest isoelectric point:
>tr|A0A4D5ZTD4|A0A4D5ZTD4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan67 OX=2558810 GN=JavanS67_0008 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.59 LPTLPEE8 pKa = 3.71 NYY10 pKa = 9.48 KK11 pKa = 10.42 RR12 pKa = 11.84 VLALIPQGAEE22 pKa = 3.44 RR23 pKa = 11.84 PRR25 pKa = 11.84 AGKK28 pKa = 10.2 EE29 pKa = 3.73 IVKK32 pKa = 8.96 LTGLDD37 pKa = 2.96 IRR39 pKa = 11.84 TVRR42 pKa = 11.84 GIIRR46 pKa = 11.84 GLVIKK51 pKa = 8.25 YY52 pKa = 9.67 HH53 pKa = 5.96 IAICGSRR60 pKa = 11.84 DD61 pKa = 3.31 LAGGYY66 pKa = 8.04 YY67 pKa = 9.75 IPANDD72 pKa = 3.72 SEE74 pKa = 4.4 RR75 pKa = 11.84 LAGIRR80 pKa = 11.84 ALDD83 pKa = 3.66 RR84 pKa = 11.84 QRR86 pKa = 11.84 QDD88 pKa = 2.9 EE89 pKa = 4.25 QKK91 pKa = 10.55 RR92 pKa = 11.84 ISALMYY98 pKa = 10.99 ANLNEE103 pKa = 3.74 QDD105 pKa = 4.18 KK106 pKa = 10.23 YY107 pKa = 11.73 LKK109 pKa = 10.86 GGG111 pKa = 3.46
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 9.37
IPC_protein 9.516
Toseland 9.94
ProMoST 9.692
Dawson 10.175
Bjellqvist 9.882
Wikipedia 10.365
Rodwell 10.467
Grimsley 10.262
Solomon 10.218
Lehninger 10.175
Nozaki 9.94
DTASelect 9.867
Thurlkill 10.014
EMBOSS 10.35
Sillero 10.087
Patrickios 9.897
IPC_peptide 10.218
IPC2_peptide 8.58
IPC2.peptide.svr19 8.358
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2735
55
382
151.9
17.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.91 ± 0.3
0.695 ± 0.209
5.338 ± 0.31
8.044 ± 0.613
3.876 ± 0.275
4.753 ± 0.481
1.536 ± 0.326
7.349 ± 0.506
9.689 ± 0.373
11.627 ± 0.776
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.354
4.205 ± 0.339
2.34 ± 0.295
5.155 ± 0.456
4.899 ± 0.557
5.009 ± 0.413
6.545 ± 0.56
4.461 ± 0.353
1.06 ± 0.205
4.168 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here