Syntrophothermus lipocalidus (strain DSM 12680 / TGB-C1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae; Syntrophothermus; Syntrophothermus lipocalidus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7CKH2|D7CKH2_SYNLT Biotin carboxylase OS=Syntrophothermus lipocalidus (strain DSM 12680 / TGB-C1) OX=643648 GN=Slip_0423 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84EE3 pKa = 4.06GGSCLKK9 pKa = 10.45NLSEE13 pKa = 5.04RR14 pKa = 11.84ISYY17 pKa = 10.6LKK19 pKa = 10.69GLSEE23 pKa = 4.93GINVAEE29 pKa = 5.57LGPQGKK35 pKa = 9.4IMNGILDD42 pKa = 3.84VLEE45 pKa = 4.35GVIDD49 pKa = 4.85DD50 pKa = 3.78ICVLRR55 pKa = 11.84TEE57 pKa = 4.03MEE59 pKa = 3.92EE60 pKa = 3.82FKK62 pKa = 10.81EE63 pKa = 4.17YY64 pKa = 10.49VQSIDD69 pKa = 4.61DD70 pKa = 4.39DD71 pKa = 4.93LNLLEE76 pKa = 4.42EE77 pKa = 5.51DD78 pKa = 4.48IYY80 pKa = 11.39GDD82 pKa = 3.48DD83 pKa = 4.44YY84 pKa = 11.65IYY86 pKa = 7.53MTCKK90 pKa = 10.01KK91 pKa = 9.83CGEE94 pKa = 4.23EE95 pKa = 4.62VYY97 pKa = 10.81FDD99 pKa = 4.51ADD101 pKa = 3.54VLEE104 pKa = 5.24DD105 pKa = 4.42DD106 pKa = 4.94DD107 pKa = 5.47VIEE110 pKa = 5.18IICPKK115 pKa = 9.63CNEE118 pKa = 4.05VVFVNDD124 pKa = 3.66GSFDD128 pKa = 3.83FEE130 pKa = 4.47PSVMEE135 pKa = 4.5SGIEE139 pKa = 4.18GEE141 pKa = 4.62NSPSPDD147 pKa = 2.78GRR149 pKa = 11.84NSGSAGNN156 pKa = 3.74

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7CKK1|D7CKK1_SYNLT Uncharacterized protein OS=Syntrophothermus lipocalidus (strain DSM 12680 / TGB-C1) OX=643648 GN=Slip_0452 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.35QPKK8 pKa = 8.47RR9 pKa = 11.84RR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.69RR14 pKa = 11.84VHH16 pKa = 5.95GFRR19 pKa = 11.84TRR21 pKa = 11.84MASAGGRR28 pKa = 11.84RR29 pKa = 11.84VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.01GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

686777

30

1888

297.2

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.546 ± 0.053

1.276 ± 0.022

4.949 ± 0.028

7.526 ± 0.056

3.66 ± 0.036

7.97 ± 0.044

1.694 ± 0.02

6.168 ± 0.038

5.302 ± 0.04

10.201 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.023

3.206 ± 0.026

4.275 ± 0.03

3.322 ± 0.03

6.442 ± 0.046

5.21 ± 0.043

4.884 ± 0.039

8.633 ± 0.046

1.064 ± 0.02

3.152 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski