Sulfurovum sp. (strain NBC37-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Sulfurovaceae; Sulfurovum; unclassified Sulfurovum

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A6QC28|RUVB_SULNB Holliday junction ATP-dependent DNA helicase RuvB OS=Sulfurovum sp. (strain NBC37-1) OX=387093 GN=ruvB PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 9.17KK3 pKa = 8.57TYY5 pKa = 10.33KK6 pKa = 10.33IILKK10 pKa = 9.93QFLFLGMMLAGSSVSAATLDD30 pKa = 3.6WNTVGWSPAGSLTQSYY46 pKa = 10.13TDD48 pKa = 3.32VDD50 pKa = 4.04SSKK53 pKa = 11.24VNIDD57 pKa = 3.1VTMTEE62 pKa = 3.81DD63 pKa = 3.19TDD65 pKa = 4.06RR66 pKa = 11.84YY67 pKa = 9.63NSGVPSVNSTYY78 pKa = 11.29GLDD81 pKa = 3.53YY82 pKa = 10.73YY83 pKa = 11.72VNYY86 pKa = 10.62SSNTQKK92 pKa = 10.54IKK94 pKa = 8.3TTITFSSPVKK104 pKa = 10.2ISFLRR109 pKa = 11.84WIDD112 pKa = 3.17IDD114 pKa = 3.85SGNPSTDD121 pKa = 3.33FDD123 pKa = 4.07DD124 pKa = 4.83RR125 pKa = 11.84VIVTAKK131 pKa = 8.82DD132 pKa = 3.28TSGATVYY139 pKa = 10.4PSSEE143 pKa = 4.13TLGDD147 pKa = 4.54HH148 pKa = 6.76IDD150 pKa = 3.5KK151 pKa = 10.9NGPGDD156 pKa = 3.89YY157 pKa = 10.53EE158 pKa = 5.12SDD160 pKa = 3.27GTTLNDD166 pKa = 4.83DD167 pKa = 4.6DD168 pKa = 5.13PDD170 pKa = 5.62GYY172 pKa = 10.06VTVAFEE178 pKa = 4.16DD179 pKa = 4.62VYY181 pKa = 10.0VTEE184 pKa = 4.5VSFDD188 pKa = 3.58YY189 pKa = 11.28TNGSNAQSDD198 pKa = 4.21PNNQGIYY205 pKa = 9.64LANITFDD212 pKa = 4.6ALDD215 pKa = 3.7SDD217 pKa = 4.47GDD219 pKa = 4.18GIADD223 pKa = 4.09VKK225 pKa = 11.31DD226 pKa = 3.38IDD228 pKa = 5.15DD229 pKa = 5.13DD230 pKa = 4.31NDD232 pKa = 4.58GILDD236 pKa = 3.74VDD238 pKa = 3.93EE239 pKa = 4.55STPAVTEE246 pKa = 4.35SNTASGTIPDD256 pKa = 3.55NGYY259 pKa = 9.82PNTCLDD265 pKa = 3.69RR266 pKa = 11.84TFDD269 pKa = 3.61IQDD272 pKa = 3.3SGIVNDD278 pKa = 3.51VTIKK282 pKa = 10.72VDD284 pKa = 3.86IAHH287 pKa = 6.84TWRR290 pKa = 11.84NDD292 pKa = 3.83LIVQLISPFGTAVDD306 pKa = 5.06LIRR309 pKa = 11.84NEE311 pKa = 4.33GGSHH315 pKa = 6.3NNLSATFFDD324 pKa = 4.23AATTSIVGDD333 pKa = 3.7TTDD336 pKa = 3.59FTLGSFLPRR345 pKa = 11.84QPEE348 pKa = 3.91QALSAFNGEE357 pKa = 4.55DD358 pKa = 3.66PQGTWTLHH366 pKa = 5.13MCDD369 pKa = 5.31DD370 pKa = 4.65ASQDD374 pKa = 2.98TGTFNEE380 pKa = 4.2ANLTINYY387 pKa = 9.36VNEE390 pKa = 4.33LDD392 pKa = 4.13SDD394 pKa = 3.75NDD396 pKa = 4.77GIIDD400 pKa = 4.69CLDD403 pKa = 4.03LDD405 pKa = 4.48SDD407 pKa = 4.52NDD409 pKa = 4.72GIPDD413 pKa = 3.58NVEE416 pKa = 3.93AQPTATYY423 pKa = 10.48DD424 pKa = 3.7EE425 pKa = 5.6PDD427 pKa = 4.04SAWADD432 pKa = 3.26ADD434 pKa = 3.89GDD436 pKa = 4.24GLADD440 pKa = 4.82QYY442 pKa = 11.38DD443 pKa = 4.08TDD445 pKa = 3.85SGGTAVTPSDD455 pKa = 3.74TDD457 pKa = 3.44GDD459 pKa = 4.59GIPDD463 pKa = 3.91YY464 pKa = 11.22LDD466 pKa = 3.81SDD468 pKa = 4.39SDD470 pKa = 3.74NDD472 pKa = 4.29GYY474 pKa = 9.68TDD476 pKa = 3.84CEE478 pKa = 4.31EE479 pKa = 4.53GNSVATGCPITPGDD493 pKa = 3.67TASTKK498 pKa = 10.13TGLVSWAQGSLGDD511 pKa = 4.52VYY513 pKa = 11.04WDD515 pKa = 3.63TTNSASVSNGNVDD528 pKa = 4.8DD529 pKa = 4.84PSNDD533 pKa = 3.31LFNEE537 pKa = 4.22TGDD540 pKa = 3.68TSEE543 pKa = 3.64VGYY546 pKa = 10.99RR547 pKa = 11.84EE548 pKa = 3.96FLCGKK553 pKa = 9.57NRR555 pKa = 11.84TTLTHH560 pKa = 5.86FQWKK564 pKa = 9.23IVSFCCATGSNHH576 pKa = 6.93IEE578 pKa = 4.19DD579 pKa = 5.0LLGGDD584 pKa = 4.21LGAYY588 pKa = 6.49GTDD591 pKa = 2.21WVVFKK596 pKa = 11.02QSGTDD601 pKa = 3.17QYY603 pKa = 11.16EE604 pKa = 4.15INSGHH609 pKa = 6.84KK610 pKa = 9.0NTTKK614 pKa = 10.73AQLAATDD621 pKa = 3.76TVVPGKK627 pKa = 10.3GYY629 pKa = 9.86WIIADD634 pKa = 3.94LGGAGNEE641 pKa = 4.09KK642 pKa = 10.71NITIDD647 pKa = 3.22KK648 pKa = 9.06TLSNLSPASTVTSSSVGITNPDD670 pKa = 3.01FTEE673 pKa = 3.81VHH675 pKa = 6.68EE676 pKa = 4.27YY677 pKa = 10.89LLPKK681 pKa = 10.7NEE683 pKa = 3.92VSDD686 pKa = 4.66PNTVDD691 pKa = 3.16YY692 pKa = 10.68KK693 pKa = 11.22KK694 pKa = 11.27YY695 pKa = 9.01MAGNPFPYY703 pKa = 10.29AFQLSDD709 pKa = 4.87LYY711 pKa = 10.94FKK713 pKa = 11.07HH714 pKa = 6.28NAGGMSYY721 pKa = 11.3NEE723 pKa = 4.53MGNTANDD730 pKa = 3.19NYY732 pKa = 10.27INKK735 pKa = 9.18IVYY738 pKa = 9.71KK739 pKa = 10.25HH740 pKa = 6.83DD741 pKa = 4.18SNKK744 pKa = 9.25TGPVSGYY751 pKa = 9.09MAVDD755 pKa = 3.8PATPGFDD762 pKa = 4.17GSIQPMEE769 pKa = 4.04GFFIKK774 pKa = 9.94IEE776 pKa = 4.03KK777 pKa = 9.66NQTDD781 pKa = 3.38NYY783 pKa = 9.06VNHH786 pKa = 6.65FAYY789 pKa = 10.0PLMNKK794 pKa = 9.64

Molecular weight: 85.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A6QAM9|DAPD_SULNB 2 3 4 5-tetrahydropyridine-2 6-dicarboxylate N-succinyltransferase OS=Sulfurovum sp. (strain NBC37-1) OX=387093 GN=dapD PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.34RR14 pKa = 11.84THH16 pKa = 6.22GFRR19 pKa = 11.84TRR21 pKa = 11.84MKK23 pKa = 8.87TKK25 pKa = 10.11NGRR28 pKa = 11.84KK29 pKa = 9.33VISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2433

0

2433

765727

37

2052

314.7

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.369 ± 0.049

0.867 ± 0.015

5.628 ± 0.038

7.189 ± 0.046

4.713 ± 0.035

6.413 ± 0.048

2.193 ± 0.025

7.652 ± 0.045

8.08 ± 0.061

9.686 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.026

4.259 ± 0.041

3.45 ± 0.027

3.007 ± 0.026

3.983 ± 0.037

5.928 ± 0.04

5.258 ± 0.038

6.499 ± 0.046

0.95 ± 0.015

3.979 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski