Nostoc sp. (strain ATCC 29411 / PCC 7524)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; unclassified Nostoc

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9QX42|K9QX42_NOSS7 Anti-sigma factor antagonist OS=Nostoc sp. (strain ATCC 29411 / PCC 7524) OX=28072 GN=Nos7524_4237 PE=3 SV=1
MM1 pKa = 6.37TTYY4 pKa = 10.74SGFPDD9 pKa = 4.28PGSSTSTALDD19 pKa = 3.74TTSLSYY25 pKa = 10.56IGGSLLSTPSIFQDD39 pKa = 3.36SVSRR43 pKa = 11.84LSPSDD48 pKa = 3.24LSDD51 pKa = 3.39YY52 pKa = 11.35YY53 pKa = 11.1RR54 pKa = 11.84FTVSSSSIVTLEE66 pKa = 4.73LDD68 pKa = 4.0GLTDD72 pKa = 3.53NANLFLRR79 pKa = 11.84NSAGSSIAPSTNAGTTAEE97 pKa = 4.65RR98 pKa = 11.84IRR100 pKa = 11.84FSISDD105 pKa = 3.64PPSNPFYY112 pKa = 11.05AHH114 pKa = 6.07VFIPNGSTSTPYY126 pKa = 10.99NLRR129 pKa = 11.84LSAEE133 pKa = 4.66TIQEE137 pKa = 4.21SYY139 pKa = 10.89ISDD142 pKa = 3.67SLIGNSRR149 pKa = 11.84NIGTFSTTDD158 pKa = 4.11GITPIIDD165 pKa = 3.69YY166 pKa = 10.61VSSTGQVVDD175 pKa = 3.46QNDD178 pKa = 4.31YY179 pKa = 11.72YY180 pKa = 11.77SFTLSNSGTVDD191 pKa = 3.02INLSSLNGTDD201 pKa = 3.46ILYY204 pKa = 10.7ADD206 pKa = 4.37LQLINSFNSVLQTSANVGTTSEE228 pKa = 4.63LINYY232 pKa = 8.7SLAAGTYY239 pKa = 8.07YY240 pKa = 9.96IRR242 pKa = 11.84AYY244 pKa = 10.69SLSDD248 pKa = 3.27PGNYY252 pKa = 9.21QLNFNFTADD261 pKa = 4.01PPDD264 pKa = 3.24QGGNTVGTATPIILDD279 pKa = 3.66TTITDD284 pKa = 3.77QVSLADD290 pKa = 3.51NSDD293 pKa = 3.89YY294 pKa = 11.5YY295 pKa = 11.44QFTLASTSLVDD306 pKa = 4.48IRR308 pKa = 11.84FTSLTADD315 pKa = 3.32ANLFLQNASGGNIITSTQPGTALDD339 pKa = 3.89AVRR342 pKa = 11.84LSLNPGTYY350 pKa = 10.24NILVNRR356 pKa = 11.84GSTQTAEE363 pKa = 4.26YY364 pKa = 8.77TLSASAQVIGTDD376 pKa = 3.3QAPNSTAIALNLGNIIGATSRR397 pKa = 11.84NEE399 pKa = 3.85FVGNLDD405 pKa = 3.36TNDD408 pKa = 3.54FYY410 pKa = 11.82KK411 pKa = 10.19FTLDD415 pKa = 3.0TTTNFSLNLNILSSYY430 pKa = 10.96FNAQLVNADD439 pKa = 3.71VQLLTSNGTQIAISNQPGNANEE461 pKa = 4.78SINTTLDD468 pKa = 2.79AGTYY472 pKa = 8.88FIRR475 pKa = 11.84VYY477 pKa = 8.51TTGLANTFYY486 pKa = 11.06DD487 pKa = 4.36LDD489 pKa = 3.66ITATPQAKK497 pKa = 9.5LVQNINPTGSSNPANLTALGSLVYY521 pKa = 8.09FTANDD526 pKa = 3.93GVNGVQLWRR535 pKa = 11.84SDD537 pKa = 3.38GNTATSLSNISSFNPNNLVVFNNRR561 pKa = 11.84LYY563 pKa = 9.88FTASDD568 pKa = 3.63STFGRR573 pKa = 11.84EE574 pKa = 3.32LWEE577 pKa = 4.09YY578 pKa = 11.23NGTSVNRR585 pKa = 11.84ISDD588 pKa = 3.71INVGAGNSDD597 pKa = 3.66PGNLTVVGNKK607 pKa = 10.01LFFTAVDD614 pKa = 3.31SSTTRR619 pKa = 11.84KK620 pKa = 9.23LWVYY624 pKa = 10.93NGTSVNLVDD633 pKa = 4.56INPGFATTGTPTFTTAFNNRR653 pKa = 11.84LFFTTQNNSQLWSTDD668 pKa = 2.67GTVGGTQVINAGGVTNSTPRR688 pKa = 11.84SLTVVGNTLYY698 pKa = 9.09FTANNGTSGHH708 pKa = 5.92EE709 pKa = 3.78VWQYY713 pKa = 11.33QSGTTASLVEE723 pKa = 5.67DD724 pKa = 3.74ITPGNNSYY732 pKa = 11.13APEE735 pKa = 4.4RR736 pKa = 11.84LTTVGNMLYY745 pKa = 10.44FVTDD749 pKa = 3.24SDD751 pKa = 4.23NDD753 pKa = 3.51FTLEE757 pKa = 3.93LWRR760 pKa = 11.84SDD762 pKa = 3.09GTEE765 pKa = 3.81SGTQIIGTDD774 pKa = 3.47GQAPNLGFGAINLTAVGNTLYY795 pKa = 10.51FVANDD800 pKa = 3.78PVSGLEE806 pKa = 3.75LWKK809 pKa = 9.73TDD811 pKa = 3.13GTDD814 pKa = 2.86VGTVIVKK821 pKa = 10.32DD822 pKa = 3.68ISSGTANSLPTGLVNFNGTLAFAASDD848 pKa = 3.59GTNRR852 pKa = 11.84EE853 pKa = 3.77VWFSDD858 pKa = 2.96GTEE861 pKa = 4.17LNTIRR866 pKa = 11.84ASNIYY871 pKa = 9.56PGGIANPDD879 pKa = 3.18WLTVVGTKK887 pKa = 10.62LFFTATDD894 pKa = 3.8GADD897 pKa = 3.36NTEE900 pKa = 4.37LYY902 pKa = 10.92VLL904 pKa = 4.45

Molecular weight: 96.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9QUY1|K9QUY1_NOSS7 Phosphate acyltransferase OS=Nostoc sp. (strain ATCC 29411 / PCC 7524) OX=28072 GN=plsX PE=3 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.01KK37 pKa = 9.6GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5398

0

5398

1809286

29

11414

335.2

37.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.217 ± 0.045

0.929 ± 0.018

4.934 ± 0.045

5.975 ± 0.048

3.847 ± 0.026

6.813 ± 0.073

1.857 ± 0.025

6.913 ± 0.026

4.481 ± 0.044

10.802 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.779 ± 0.024

4.667 ± 0.064

4.717 ± 0.042

5.58 ± 0.048

4.947 ± 0.04

6.277 ± 0.04

6.072 ± 0.077

6.723 ± 0.031

1.401 ± 0.021

3.07 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski