Amino acid dipepetide frequency for Nostoc sp. (strain ATCC 29411 / PCC 7524)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.782AlaAla: 7.782 ± 0.102	0.767AlaCys: 0.767 ± 0.022	4.207AlaAsp: 4.207 ± 0.071	5.115AlaGlu: 5.115 ± 0.068	2.871AlaPhe: 2.871 ± 0.05	5.696AlaGly: 5.696 ± 0.111	1.322AlaHis: 1.322 ± 0.029	6.857AlaIle: 6.857 ± 0.072	3.953AlaLys: 3.953 ± 0.059	8.31AlaLeu: 8.31 ± 0.089	1.594AlaMet: 1.594 ± 0.034	3.757AlaAsn: 3.757 ± 0.078	2.995AlaPro: 2.995 ± 0.062	4.278AlaGln: 4.278 ± 0.064	3.471AlaArg: 3.471 ± 0.054	4.761AlaSer: 4.761 ± 0.057	5.143AlaThr: 5.143 ± 0.095	5.87AlaVal: 5.87 ± 0.083	1.025AlaTrp: 1.025 ± 0.028	2.396AlaTyr: 2.396 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.607CysAla: 0.607 ± 0.021	0.162CysCys: 0.162 ± 0.011	0.522CysAsp: 0.522 ± 0.021	0.487CysGlu: 0.487 ± 0.019	0.372CysPhe: 0.372 ± 0.016	0.768CysGly: 0.768 ± 0.025	0.288CysHis: 0.288 ± 0.015	0.549CysIle: 0.549 ± 0.02	0.298CysLys: 0.298 ± 0.013	1.103CysLeu: 1.103 ± 0.033	0.148CysMet: 0.148 ± 0.01	0.361CysAsn: 0.361 ± 0.015	0.499CysPro: 0.499 ± 0.021	0.597CysGln: 0.597 ± 0.022	0.48CysArg: 0.48 ± 0.019	0.549CysSer: 0.549 ± 0.02	0.451CysThr: 0.451 ± 0.019	0.563CysVal: 0.563 ± 0.018	0.149CysTrp: 0.149 ± 0.01	0.334CysTyr: 0.334 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.873AspAla: 3.873 ± 0.083	0.491AspCys: 0.491 ± 0.019	2.329AspAsp: 2.329 ± 0.048	2.977AspGlu: 2.977 ± 0.045	2.287AspPhe: 2.287 ± 0.036	3.573AspGly: 3.573 ± 0.097	0.776AspHis: 0.776 ± 0.022	3.722AspIle: 3.722 ± 0.065	2.141AspLys: 2.141 ± 0.045	5.461AspLeu: 5.461 ± 0.058	0.742AspMet: 0.742 ± 0.022	2.249AspAsn: 2.249 ± 0.058	2.298AspPro: 2.298 ± 0.059	1.766AspGln: 1.766 ± 0.031	2.909AspArg: 2.909 ± 0.047	2.954AspSer: 2.954 ± 0.057	2.883AspThr: 2.883 ± 0.076	3.192AspVal: 3.192 ± 0.051	0.9AspTrp: 0.9 ± 0.027	1.816AspTyr: 1.816 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.063GluAla: 5.063 ± 0.064	0.469GluCys: 0.469 ± 0.018	2.581GluAsp: 2.581 ± 0.037	3.636GluGlu: 3.636 ± 0.068	2.411GluPhe: 2.411 ± 0.043	3.026GluGly: 3.026 ± 0.04	0.988GluHis: 0.988 ± 0.027	4.982GluIle: 4.982 ± 0.061	3.185GluLys: 3.185 ± 0.064	6.788GluLeu: 6.788 ± 0.084	1.266GluMet: 1.266 ± 0.037	2.741GluAsn: 2.741 ± 0.045	2.313GluPro: 2.313 ± 0.049	3.609GluGln: 3.609 ± 0.059	3.24GluArg: 3.24 ± 0.06	3.184GluSer: 3.184 ± 0.05	3.409GluThr: 3.409 ± 0.046	4.163GluVal: 4.163 ± 0.056	0.76GluTrp: 0.76 ± 0.018	1.939GluTyr: 1.939 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.107PheAla: 3.107 ± 0.053	0.492PheCys: 0.492 ± 0.019	2.18PheAsp: 2.18 ± 0.042	1.96PheGlu: 1.96 ± 0.034	1.583PhePhe: 1.583 ± 0.034	2.935PheGly: 2.935 ± 0.046	0.758PheHis: 0.758 ± 0.024	2.456PheIle: 2.456 ± 0.041	1.439PheLys: 1.439 ± 0.033	3.948PheLeu: 3.948 ± 0.063	0.678PheMet: 0.678 ± 0.021	1.845PheAsn: 1.845 ± 0.041	1.793PhePro: 1.793 ± 0.04	1.848PheGln: 1.848 ± 0.035	1.729PheArg: 1.729 ± 0.034	2.786PheSer: 2.786 ± 0.04	2.463PheThr: 2.463 ± 0.051	2.427PheVal: 2.427 ± 0.041	0.681PheTrp: 0.681 ± 0.021	1.362PheTyr: 1.362 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.045GlyAla: 5.045 ± 0.122	0.775GlyCys: 0.775 ± 0.027	3.541GlyAsp: 3.541 ± 0.081	4.149GlyGlu: 4.149 ± 0.058	2.886GlyPhe: 2.886 ± 0.039	5.15GlyGly: 5.15 ± 0.162	1.178GlyHis: 1.178 ± 0.036	5.148GlyIle: 5.148 ± 0.062	3.834GlyLys: 3.834 ± 0.056	6.99GlyLeu: 6.99 ± 0.072	1.467GlyMet: 1.467 ± 0.035	3.641GlyAsn: 3.641 ± 0.12	1.195GlyPro: 1.195 ± 0.029	2.985GlyGln: 2.985 ± 0.052	3.312GlyArg: 3.312 ± 0.058	4.199GlySer: 4.199 ± 0.103	4.259GlyThr: 4.259 ± 0.124	4.92GlyVal: 4.92 ± 0.066	1.076GlyTrp: 1.076 ± 0.026	2.376GlyTyr: 2.376 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	1.074HisAla: 1.074 ± 0.03	0.235HisCys: 0.235 ± 0.011	0.79HisAsp: 0.79 ± 0.023	0.953HisGlu: 0.953 ± 0.027	0.746HisPhe: 0.746 ± 0.026	1.144HisGly: 1.144 ± 0.026	0.656HisHis: 0.656 ± 0.026	1.157HisIle: 1.157 ± 0.027	0.785HisLys: 0.785 ± 0.023	2.215HisLeu: 2.215 ± 0.044	0.259HisMet: 0.259 ± 0.015	0.813HisAsn: 0.813 ± 0.023	1.375HisPro: 1.375 ± 0.034	1.263HisGln: 1.263 ± 0.036	1.068HisArg: 1.068 ± 0.025	1.168HisSer: 1.168 ± 0.033	1.01HisThr: 1.01 ± 0.025	0.803HisVal: 0.803 ± 0.023	0.363HisTrp: 0.363 ± 0.016	0.689HisTyr: 0.689 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.238IleAla: 7.238 ± 0.066	0.701IleCys: 0.701 ± 0.023	3.863IleAsp: 3.863 ± 0.077	4.078IleGlu: 4.078 ± 0.051	2.606IlePhe: 2.606 ± 0.04	4.569IleGly: 4.569 ± 0.059	1.297IleHis: 1.297 ± 0.03	4.041IleIle: 4.041 ± 0.051	3.128IleLys: 3.128 ± 0.052	6.871IleLeu: 6.871 ± 0.089	0.897IleMet: 0.897 ± 0.03	3.611IleAsn: 3.611 ± 0.082	3.66IlePro: 3.66 ± 0.061	3.199IleGln: 3.199 ± 0.044	3.096IleArg: 3.096 ± 0.042	4.566IleSer: 4.566 ± 0.065	4.403IleThr: 4.403 ± 0.09	4.349IleVal: 4.349 ± 0.053	0.876IleTrp: 0.876 ± 0.028	2.081IleTyr: 2.081 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	3.731LysAla: 3.731 ± 0.053	0.323LysCys: 0.323 ± 0.016	1.96LysAsp: 1.96 ± 0.041	2.448LysGlu: 2.448 ± 0.054	1.755LysPhe: 1.755 ± 0.042	2.388LysGly: 2.388 ± 0.036	0.816LysHis: 0.816 ± 0.022	3.451LysIle: 3.451 ± 0.056	2.061LysLys: 2.061 ± 0.05	5.261LysLeu: 5.261 ± 0.076	0.87LysMet: 0.87 ± 0.025	2.044LysAsn: 2.044 ± 0.044	2.303LysPro: 2.303 ± 0.048	2.622LysGln: 2.622 ± 0.049	2.137LysArg: 2.137 ± 0.048	2.856LysSer: 2.856 ± 0.049	2.84LysThr: 2.84 ± 0.043	3.053LysVal: 3.053 ± 0.043	0.452LysTrp: 0.452 ± 0.02	1.44LysTyr: 1.44 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	9.756LeuAla: 9.756 ± 0.108	0.915LeuCys: 0.915 ± 0.027	5.177LeuAsp: 5.177 ± 0.067	6.92LeuGlu: 6.92 ± 0.092	3.653LeuPhe: 3.653 ± 0.058	7.676LeuGly: 7.676 ± 0.078	1.912LeuHis: 1.912 ± 0.044	6.807LeuIle: 6.807 ± 0.076	5.072LeuLys: 5.072 ± 0.079	11.132LeuLeu: 11.132 ± 0.131	2.005LeuMet: 2.005 ± 0.046	4.627LeuAsn: 4.627 ± 0.061	5.691LeuPro: 5.691 ± 0.08	6.148LeuGln: 6.148 ± 0.089	5.433LeuArg: 5.433 ± 0.079	7.232LeuSer: 7.232 ± 0.058	6.609LeuThr: 6.609 ± 0.078	7.112LeuVal: 7.112 ± 0.08	1.463LeuTrp: 1.463 ± 0.046	2.677LeuTyr: 2.677 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.619MetAla: 1.619 ± 0.038	0.127MetCys: 0.127 ± 0.009	0.681MetAsp: 0.681 ± 0.017	0.914MetGlu: 0.914 ± 0.023	0.549MetPhe: 0.549 ± 0.017	1.318MetGly: 1.318 ± 0.031	0.243MetHis: 0.243 ± 0.012	1.095MetIle: 1.095 ± 0.03	0.888MetLys: 0.888 ± 0.026	1.861MetLeu: 1.861 ± 0.043	0.454MetMet: 0.454 ± 0.018	0.858MetAsn: 0.858 ± 0.021	0.888MetPro: 0.888 ± 0.027	0.994MetGln: 0.994 ± 0.025	0.96MetArg: 0.96 ± 0.024	1.296MetSer: 1.296 ± 0.031	1.268MetThr: 1.268 ± 0.033	1.219MetVal: 1.219 ± 0.031	0.156MetTrp: 0.156 ± 0.009	0.4MetTyr: 0.4 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.151AsnAla: 3.151 ± 0.084	0.45AsnCys: 0.45 ± 0.02	1.931AsnAsp: 1.931 ± 0.055	1.827AsnGlu: 1.827 ± 0.035	2.104AsnPhe: 2.104 ± 0.044	2.957AsnGly: 2.957 ± 0.09	1.035AsnHis: 1.035 ± 0.025	3.176AsnIle: 3.176 ± 0.066	1.64AsnLys: 1.64 ± 0.036	5.75AsnLeu: 5.75 ± 0.072	0.59AsnMet: 0.59 ± 0.016	2.493AsnAsn: 2.493 ± 0.088	3.071AsnPro: 3.071 ± 0.05	3.014AsnGln: 3.014 ± 0.055	2.409AsnArg: 2.409 ± 0.044	3.253AsnSer: 3.253 ± 0.075	2.887AsnThr: 2.887 ± 0.091	2.533AsnVal: 2.533 ± 0.062	0.774AsnTrp: 0.774 ± 0.023	1.627AsnTyr: 1.627 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	3.297ProAla: 3.297 ± 0.057	0.345ProCys: 0.345 ± 0.015	2.943ProAsp: 2.943 ± 0.056	3.88ProGlu: 3.88 ± 0.065	1.633ProPhe: 1.633 ± 0.038	3.148ProGly: 3.148 ± 0.056	0.962ProHis: 0.962 ± 0.024	3.143ProIle: 3.143 ± 0.05	2.052ProLys: 2.052 ± 0.047	4.549ProLeu: 4.549 ± 0.062	0.707ProMet: 0.707 ± 0.023	2.285ProAsn: 2.285 ± 0.046	2.338ProPro: 2.338 ± 0.055	2.818ProGln: 2.818 ± 0.053	1.698ProArg: 1.698 ± 0.038	2.903ProSer: 2.903 ± 0.05	3.112ProThr: 3.112 ± 0.052	3.42ProVal: 3.42 ± 0.055	0.604ProTrp: 0.604 ± 0.021	1.329ProTyr: 1.329 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	5.047GlnAla: 5.047 ± 0.072	0.355GlnCys: 0.355 ± 0.015	2.302GlnAsp: 2.302 ± 0.035	3.654GlnGlu: 3.654 ± 0.058	1.769GlnPhe: 1.769 ± 0.032	3.435GlnGly: 3.435 ± 0.051	0.915GlnHis: 0.915 ± 0.026	3.868GlnIle: 3.868 ± 0.056	2.695GlnLys: 2.695 ± 0.053	6.204GlnLeu: 6.204 ± 0.087	1.124GlnMet: 1.124 ± 0.03	2.29GlnAsn: 2.29 ± 0.04	2.846GlnPro: 2.846 ± 0.05	4.152GlnGln: 4.152 ± 0.076	2.955GlnArg: 2.955 ± 0.052	3.013GlnSer: 3.013 ± 0.044	3.159GlnThr: 3.159 ± 0.048	4.002GlnVal: 4.002 ± 0.053	0.659GlnTrp: 0.659 ± 0.022	1.358GlnTyr: 1.358 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.138ArgAla: 3.138 ± 0.048	0.475ArgCys: 0.475 ± 0.017	2.53ArgAsp: 2.53 ± 0.04	3.07ArgGlu: 3.07 ± 0.055	2.085ArgPhe: 2.085 ± 0.04	2.961ArgGly: 2.961 ± 0.045	0.99ArgHis: 0.99 ± 0.032	3.256ArgIle: 3.256 ± 0.041	2.039ArgLys: 2.039 ± 0.044	5.859ArgLeu: 5.859 ± 0.074	0.958ArgMet: 0.958 ± 0.024	2.194ArgAsn: 2.194 ± 0.032	1.973ArgPro: 1.973 ± 0.042	3.281ArgGln: 3.281 ± 0.06	2.963ArgArg: 2.963 ± 0.063	3.015ArgSer: 3.015 ± 0.053	2.563ArgThr: 2.563 ± 0.037	3.437ArgVal: 3.437 ± 0.051	0.809ArgTrp: 0.809 ± 0.026	1.871ArgTyr: 1.871 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.448SerAla: 4.448 ± 0.065	0.58SerCys: 0.58 ± 0.023	3.215SerAsp: 3.215 ± 0.059	3.413SerGlu: 3.413 ± 0.052	2.447SerPhe: 2.447 ± 0.04	4.787SerGly: 4.787 ± 0.091	1.334SerHis: 1.334 ± 0.031	3.89SerIle: 3.89 ± 0.067	2.408SerLys: 2.408 ± 0.04	7.184SerLeu: 7.184 ± 0.078	1.094SerMet: 1.094 ± 0.026	2.895SerAsn: 2.895 ± 0.068	3.475SerPro: 3.475 ± 0.051	3.719SerGln: 3.719 ± 0.053	3.133SerArg: 3.133 ± 0.05	4.306SerSer: 4.306 ± 0.077	3.551SerThr: 3.551 ± 0.072	4.15SerVal: 4.15 ± 0.062	0.927SerTrp: 0.927 ± 0.027	1.807SerTyr: 1.807 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	5.318ThrAla: 5.318 ± 0.092	0.438ThrCys: 0.438 ± 0.017	2.971ThrAsp: 2.971 ± 0.072	3.327ThrGlu: 3.327 ± 0.051	2.254ThrPhe: 2.254 ± 0.05	4.82ThrGly: 4.82 ± 0.104	1.057ThrHis: 1.057 ± 0.02	4.102ThrIle: 4.102 ± 0.103	2.335ThrLys: 2.335 ± 0.039	6.498ThrLeu: 6.498 ± 0.089	0.817ThrMet: 0.817 ± 0.024	2.71ThrAsn: 2.71 ± 0.092	3.806ThrPro: 3.806 ± 0.067	3.084ThrGln: 3.084 ± 0.05	2.469ThrArg: 2.469 ± 0.048	3.648ThrSer: 3.648 ± 0.073	4.124ThrThr: 4.124 ± 0.118	4.439ThrVal: 4.439 ± 0.103	0.719ThrTrp: 0.719 ± 0.023	1.781ThrTyr: 1.781 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	5.976ValAla: 5.976 ± 0.071	0.659ValCys: 0.659 ± 0.025	3.433ValAsp: 3.433 ± 0.059	4.315ValGlu: 4.315 ± 0.055	2.581ValPhe: 2.581 ± 0.04	4.766ValGly: 4.766 ± 0.067	1.005ValHis: 1.005 ± 0.025	4.718ValIle: 4.718 ± 0.053	3.21ValLys: 3.21 ± 0.047	6.527ValLeu: 6.527 ± 0.076	1.401ValMet: 1.401 ± 0.03	3.384ValAsn: 3.384 ± 0.075	2.756ValPro: 2.756 ± 0.053	2.95ValGln: 2.95 ± 0.042	3.218ValArg: 3.218 ± 0.046	4.318ValSer: 4.318 ± 0.062	4.259ValThr: 4.259 ± 0.091	4.991ValVal: 4.991 ± 0.059	0.874ValTrp: 0.874 ± 0.026	1.892ValTyr: 1.892 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.8TrpAla: 0.8 ± 0.023	0.146TrpCys: 0.146 ± 0.009	0.645TrpAsp: 0.645 ± 0.026	0.909TrpGlu: 0.909 ± 0.023	0.554TrpPhe: 0.554 ± 0.02	0.958TrpGly: 0.958 ± 0.025	0.348TrpHis: 0.348 ± 0.015	0.819TrpIle: 0.819 ± 0.023	0.496TrpLys: 0.496 ± 0.021	1.922TrpLeu: 1.922 ± 0.047	0.306TrpMet: 0.306 ± 0.014	0.622TrpAsn: 0.622 ± 0.021	0.298TrpPro: 0.298 ± 0.015	1.322TrpGln: 1.322 ± 0.03	0.827TrpArg: 0.827 ± 0.023	0.802TrpSer: 0.802 ± 0.024	0.626TrpThr: 0.626 ± 0.024	0.943TrpVal: 0.943 ± 0.024	0.235TrpTrp: 0.235 ± 0.013	0.429TrpTyr: 0.429 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.096TyrAla: 2.096 ± 0.033	0.383TyrCys: 0.383 ± 0.017	1.54TyrAsp: 1.54 ± 0.062	1.718TyrGlu: 1.718 ± 0.036	1.324TyrPhe: 1.324 ± 0.034	2.051TyrGly: 2.051 ± 0.037	0.686TyrHis: 0.686 ± 0.019	1.896TyrIle: 1.896 ± 0.037	1.151TyrLys: 1.151 ± 0.032	3.585TyrLeu: 3.585 ± 0.053	0.412TyrMet: 0.412 ± 0.016	1.249TyrAsn: 1.249 ± 0.034	1.596TyrPro: 1.596 ± 0.034	2.152TyrGln: 2.152 ± 0.039	1.96TyrArg: 1.96 ± 0.037	1.955TyrSer: 1.955 ± 0.037	1.698TyrThr: 1.698 ± 0.052	1.648TyrVal: 1.648 ± 0.034	0.506TyrTrp: 0.506 ± 0.017	1.097TyrTyr: 1.097 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5398 proteins (1809286 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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