Caldisalinibacter kiritimatiensis
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2681 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R1AUE1|R1AUE1_9CLOT Maltodextrin-binding protein OS=Caldisalinibacter kiritimatiensis OX=1304284 GN=L21TH_1154 PE=3 SV=1
MM1 pKa = 7.74 EE2 pKa = 4.44 IAIEE6 pKa = 4.16 KK7 pKa = 10.21 FVDD10 pKa = 4.39 VYY12 pKa = 10.39 EE13 pKa = 4.24 QPPDD17 pKa = 3.15 IYY19 pKa = 10.98 EE20 pKa = 4.06 LDD22 pKa = 3.6 KK23 pKa = 11.74 VSFTDD28 pKa = 3.12 WTSPNTCDD36 pKa = 4.33 YY37 pKa = 10.91 CDD39 pKa = 3.94 KK40 pKa = 10.76 APKK43 pKa = 9.99 YY44 pKa = 10.67 LVVV47 pKa = 3.59
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 4.05
IPC_protein 3.91
Toseland 3.719
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.037
Patrickios 0.693
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|R1AQX0|R1AQX0_9CLOT Uncharacterized protein OS=Caldisalinibacter kiritimatiensis OX=1304284 GN=L21TH_2429 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.0 KK9 pKa = 7.87 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.7 RR21 pKa = 11.84 MKK23 pKa = 8.28 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 KK29 pKa = 6.71 VLKK32 pKa = 9.34 RR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 8.76 RR36 pKa = 11.84 KK37 pKa = 8.67 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.363
IPC2_protein 10.935
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.062
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.009
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2681
0
2681
782275
37
2029
291.8
33.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.488 ± 0.049
0.872 ± 0.017
5.548 ± 0.039
7.841 ± 0.062
4.109 ± 0.038
6.444 ± 0.044
1.429 ± 0.019
10.168 ± 0.061
9.339 ± 0.06
9.044 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.483 ± 0.026
6.004 ± 0.053
3.006 ± 0.026
2.449 ± 0.023
3.663 ± 0.033
5.549 ± 0.033
4.937 ± 0.033
6.819 ± 0.043
0.689 ± 0.017
4.117 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here