Streptococcus satellite phage Javan594
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZS95|A0A4D5ZS95_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan594 OX=2558764 GN=JavanS594_0010 PE=4 SV=1
MM1 pKa = 7.07 LTFRR5 pKa = 11.84 EE6 pKa = 4.49 LEE8 pKa = 4.74 HH9 pKa = 7.5 IAEE12 pKa = 5.05 TILKK16 pKa = 8.06 HH17 pKa = 4.55 TTPEE21 pKa = 3.77 EE22 pKa = 4.05 MQCYY26 pKa = 10.38 LDD28 pKa = 4.3 MEE30 pKa = 5.25 HH31 pKa = 7.69 DD32 pKa = 5.45 SKK34 pKa = 11.62 LLWIKK39 pKa = 10.53 YY40 pKa = 9.44 KK41 pKa = 10.32 IASLEE46 pKa = 4.14 VQVV49 pKa = 4.4
Molecular weight: 5.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.056
IPC2_protein 5.169
IPC_protein 5.003
Toseland 5.029
ProMoST 5.156
Dawson 5.016
Bjellqvist 5.13
Wikipedia 4.876
Rodwell 4.965
Grimsley 4.978
Solomon 5.016
Lehninger 4.978
Nozaki 5.156
DTASelect 5.232
Thurlkill 5.041
EMBOSS 4.94
Sillero 5.245
Patrickios 3.834
IPC_peptide 5.029
IPC2_peptide 5.245
IPC2.peptide.svr19 5.202
Protein with the highest isoelectric point:
>tr|A0A4D5ZRE9|A0A4D5ZRE9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan594 OX=2558764 GN=JavanS594_0002 PE=4 SV=1
MM1 pKa = 7.41 TGSSSNVTGRR11 pKa = 11.84 TKK13 pKa = 10.82 KK14 pKa = 9.46 EE15 pKa = 4.12 VKK17 pKa = 10.43 NKK19 pKa = 6.58 TQQAIATFKK28 pKa = 10.34 TDD30 pKa = 2.4 GATRR34 pKa = 11.84 YY35 pKa = 9.59 QSATITSYY43 pKa = 11.51 KK44 pKa = 10.13 EE45 pKa = 4.02 LAEE48 pKa = 4.25 LWWNSYY54 pKa = 8.4 KK55 pKa = 9.73 HH56 pKa = 4.85 TVKK59 pKa = 10.64 PNTRR63 pKa = 11.84 GNIRR67 pKa = 11.84 GLLKK71 pKa = 10.74 NHH73 pKa = 6.71 VIPLFGTYY81 pKa = 10.59 KK82 pKa = 10.28 LDD84 pKa = 3.86 KK85 pKa = 10.02 LTTPLLQNIVIKK97 pKa = 10.73 LADD100 pKa = 3.5 KK101 pKa = 11.26 ANTGEE106 pKa = 4.04 AGAYY110 pKa = 8.96 LHH112 pKa = 6.6 YY113 pKa = 11.09 DD114 pKa = 4.21 KK115 pKa = 11.06 IHH117 pKa = 6.04 TLNKK121 pKa = 10.13 RR122 pKa = 11.84 ILQYY126 pKa = 10.87 GVVMQVLPYY135 pKa = 9.81 NPARR139 pKa = 11.84 EE140 pKa = 4.25 VILPRR145 pKa = 11.84 NAKK148 pKa = 9.33 KK149 pKa = 9.43 ATRR152 pKa = 11.84 KK153 pKa = 9.25 KK154 pKa = 10.03 VKK156 pKa = 10.16 HH157 pKa = 6.24 FNDD160 pKa = 3.34 EE161 pKa = 3.86 QLKK164 pKa = 10.06 QFLGYY169 pKa = 10.59 LDD171 pKa = 5.41 GLDD174 pKa = 3.23 LTNYY178 pKa = 9.48 RR179 pKa = 11.84 NIYY182 pKa = 9.9 EE183 pKa = 4.05 VTLYY187 pKa = 10.98 KK188 pKa = 10.5 FLLATGCRR196 pKa = 11.84 INEE199 pKa = 3.89 VLALHH204 pKa = 6.87 WSDD207 pKa = 4.12 IDD209 pKa = 4.14 LNNATVSITKK219 pKa = 6.06 TTKK222 pKa = 10.17 PLWVNQFPQVQMPAYY237 pKa = 10.09 AII239 pKa = 4.0
Molecular weight: 27.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.094
IPC2_protein 9.37
IPC_protein 9.282
Toseland 9.97
ProMoST 9.648
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.687
Grimsley 10.248
Solomon 10.189
Lehninger 10.16
Nozaki 9.94
DTASelect 9.838
Thurlkill 10.028
EMBOSS 10.365
Sillero 10.087
Patrickios 10.175
IPC_peptide 10.189
IPC2_peptide 8.346
IPC2.peptide.svr19 8.357
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2339
38
413
155.9
18.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.43 ± 0.462
0.812 ± 0.168
5.088 ± 0.369
8.337 ± 0.939
3.035 ± 0.346
4.703 ± 0.458
2.736 ± 0.34
6.627 ± 0.602
9.192 ± 0.893
10.774 ± 0.49
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.437 ± 0.391
5.259 ± 0.53
3.078 ± 0.392
3.805 ± 0.361
6.327 ± 0.52
5.729 ± 0.741
6.071 ± 0.532
4.446 ± 0.32
1.454 ± 0.245
4.66 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here