Streptomyces sporangiiformans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8775 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A505DM37|A0A505DM37_9ACTN Universal stress protein OS=Streptomyces sporangiiformans OX=2315329 GN=FGD71_017190 PE=3 SV=1
MM1 pKa = 7.63ALLAEE6 pKa = 4.67HH7 pKa = 7.16NITATPGRR15 pKa = 11.84RR16 pKa = 11.84VLEE19 pKa = 4.4VYY21 pKa = 10.91DD22 pKa = 4.17DD23 pKa = 3.9DD24 pKa = 6.95AYY26 pKa = 11.53LGDD29 pKa = 4.17EE30 pKa = 4.45AALDD34 pKa = 3.72AAEE37 pKa = 4.25TQVVAGNGYY46 pKa = 9.37HH47 pKa = 6.78VYY49 pKa = 10.59LLSLQPDD56 pKa = 3.88MKK58 pKa = 10.71VQMTIRR64 pKa = 11.84IWDD67 pKa = 3.94TPPDD71 pKa = 4.11PPADD75 pKa = 3.98AEE77 pKa = 4.33GHH79 pKa = 5.78TDD81 pKa = 3.15VSLEE85 pKa = 4.14SEE87 pKa = 4.11TGILVIGQLDD97 pKa = 3.83RR98 pKa = 11.84GPADD102 pKa = 4.84EE103 pKa = 4.59ITLPRR108 pKa = 11.84PGVYY112 pKa = 9.51EE113 pKa = 3.95GHH115 pKa = 6.86AWWQGRR121 pKa = 11.84QAAADD126 pKa = 4.1YY127 pKa = 11.32YY128 pKa = 10.97DD129 pKa = 3.53TTLDD133 pKa = 3.51QLTDD137 pKa = 4.13GSPEE141 pKa = 4.06DD142 pKa = 3.79QLTEE146 pKa = 3.65AWNNCPFTEE155 pKa = 5.23RR156 pKa = 11.84YY157 pKa = 9.8VLDD160 pKa = 3.7LAYY163 pKa = 9.55TRR165 pKa = 11.84EE166 pKa = 4.2PEE168 pKa = 4.47PVDD171 pKa = 4.38EE172 pKa = 6.4DD173 pKa = 4.3DD174 pKa = 4.67LL175 pKa = 6.4

Molecular weight: 19.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A505DD63|A0A505DD63_9ACTN Crp/Fnr family transcriptional regulator OS=Streptomyces sporangiiformans OX=2315329 GN=FGD71_045865 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8775

0

8775

2919780

24

5227

332.7

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.132 ± 0.035

0.79 ± 0.007

6.037 ± 0.023

5.77 ± 0.028

2.81 ± 0.015

9.369 ± 0.026

2.286 ± 0.013

3.316 ± 0.019

2.301 ± 0.023

10.255 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.788 ± 0.012

1.87 ± 0.016

5.948 ± 0.025

2.84 ± 0.017

7.866 ± 0.028

5.34 ± 0.021

6.147 ± 0.023

8.434 ± 0.023

1.554 ± 0.011

2.146 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski