Amino acid dipepetide frequency for Streptomyces sporangiiformans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.982AlaAla: 19.982 ± 0.129	1.074AlaCys: 1.074 ± 0.019	8.249AlaAsp: 8.249 ± 0.065	8.673AlaGlu: 8.673 ± 0.075	3.517AlaPhe: 3.517 ± 0.039	12.239AlaGly: 12.239 ± 0.086	2.814AlaHis: 2.814 ± 0.032	3.664AlaIle: 3.664 ± 0.038	2.899AlaLys: 2.899 ± 0.041	13.906AlaLeu: 13.906 ± 0.089	2.534AlaMet: 2.534 ± 0.033	1.952AlaAsn: 1.952 ± 0.026	6.653AlaPro: 6.653 ± 0.057	3.776AlaGln: 3.776 ± 0.039	9.789AlaArg: 9.789 ± 0.072	6.151AlaSer: 6.151 ± 0.051	6.917AlaThr: 6.917 ± 0.051	11.883AlaVal: 11.883 ± 0.082	1.858AlaTrp: 1.858 ± 0.025	2.791AlaTyr: 2.791 ± 0.034	0.002AlaXaa: 0.002 ± 0.001
Cys	1.083CysAla: 1.083 ± 0.021	0.1CysCys: 0.1 ± 0.006	0.494CysAsp: 0.494 ± 0.013	0.419CysGlu: 0.419 ± 0.014	0.214CysPhe: 0.214 ± 0.009	0.953CysGly: 0.953 ± 0.02	0.185CysHis: 0.185 ± 0.009	0.165CysIle: 0.165 ± 0.008	0.122CysLys: 0.122 ± 0.006	0.757CysLeu: 0.757 ± 0.016	0.126CysMet: 0.126 ± 0.007	0.135CysAsn: 0.135 ± 0.007	0.472CysPro: 0.472 ± 0.013	0.163CysGln: 0.163 ± 0.007	0.589CysArg: 0.589 ± 0.014	0.444CysSer: 0.444 ± 0.012	0.515CysThr: 0.515 ± 0.014	0.685CysVal: 0.685 ± 0.014	0.124CysTrp: 0.124 ± 0.007	0.152CysTyr: 0.152 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.609AspAla: 7.609 ± 0.057	0.436AspCys: 0.436 ± 0.013	3.73AspAsp: 3.73 ± 0.045	3.999AspGlu: 3.999 ± 0.047	1.715AspPhe: 1.715 ± 0.029	6.375AspGly: 6.375 ± 0.065	1.434AspHis: 1.434 ± 0.023	2.027AspIle: 2.027 ± 0.026	1.303AspLys: 1.303 ± 0.027	6.181AspLeu: 6.181 ± 0.053	0.858AspMet: 0.858 ± 0.017	1.03AspAsn: 1.03 ± 0.022	4.293AspPro: 4.293 ± 0.043	1.629AspGln: 1.629 ± 0.025	4.827AspArg: 4.827 ± 0.043	2.735AspSer: 2.735 ± 0.031	3.196AspThr: 3.196 ± 0.031	4.796AspVal: 4.796 ± 0.038	1.069AspTrp: 1.069 ± 0.019	1.122AspTyr: 1.122 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.354GluAla: 7.354 ± 0.071	0.372GluCys: 0.372 ± 0.012	2.91GluAsp: 2.91 ± 0.033	3.575GluGlu: 3.575 ± 0.043	1.524GluPhe: 1.524 ± 0.023	4.488GluGly: 4.488 ± 0.046	1.537GluHis: 1.537 ± 0.025	2.291GluIle: 2.291 ± 0.031	1.575GluLys: 1.575 ± 0.03	6.802GluLeu: 6.802 ± 0.061	0.91GluMet: 0.91 ± 0.019	1.073GluAsn: 1.073 ± 0.023	3.428GluPro: 3.428 ± 0.041	2.309GluGln: 2.309 ± 0.031	5.439GluArg: 5.439 ± 0.055	2.746GluSer: 2.746 ± 0.033	2.954GluThr: 2.954 ± 0.033	4.445GluVal: 4.445 ± 0.044	0.814GluTrp: 0.814 ± 0.019	1.154GluTyr: 1.154 ± 0.024	0.001GluXaa: 0.001 ± 0.001
Phe	3.67PheAla: 3.67 ± 0.039	0.257PheCys: 0.257 ± 0.008	1.991PheAsp: 1.991 ± 0.026	1.476PheGlu: 1.476 ± 0.024	0.914PhePhe: 0.914 ± 0.021	3.105PheGly: 3.105 ± 0.033	0.635PheHis: 0.635 ± 0.015	0.785PheIle: 0.785 ± 0.018	0.571PheLys: 0.571 ± 0.017	2.659PheLeu: 2.659 ± 0.038	0.432PheMet: 0.432 ± 0.012	0.62PheAsn: 0.62 ± 0.014	1.415PhePro: 1.415 ± 0.022	0.728PheGln: 0.728 ± 0.014	1.836PheArg: 1.836 ± 0.028	1.555PheSer: 1.555 ± 0.024	2.043PheThr: 2.043 ± 0.03	2.344PheVal: 2.344 ± 0.031	0.452PheTrp: 0.452 ± 0.014	0.617PheTyr: 0.617 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.681GlyAla: 10.681 ± 0.08	0.817GlyCys: 0.817 ± 0.02	5.156GlyAsp: 5.156 ± 0.049	5.105GlyGlu: 5.105 ± 0.046	2.964GlyPhe: 2.964 ± 0.034	8.553GlyGly: 8.553 ± 0.078	2.346GlyHis: 2.346 ± 0.033	3.581GlyIle: 3.581 ± 0.036	2.567GlyLys: 2.567 ± 0.032	9.405GlyLeu: 9.405 ± 0.071	2.03GlyMet: 2.03 ± 0.027	1.836GlyAsn: 1.836 ± 0.032	5.031GlyPro: 5.031 ± 0.051	2.748GlyGln: 2.748 ± 0.032	7.462GlyArg: 7.462 ± 0.061	5.56GlySer: 5.56 ± 0.051	6.362GlyThr: 6.362 ± 0.054	7.532GlyVal: 7.532 ± 0.062	1.68GlyTrp: 1.68 ± 0.028	2.266GlyTyr: 2.266 ± 0.031	0.002GlyXaa: 0.002 ± 0.001
His	2.643HisAla: 2.643 ± 0.032	0.21HisCys: 0.21 ± 0.009	1.333HisAsp: 1.333 ± 0.023	1.261HisGlu: 1.261 ± 0.022	0.663HisPhe: 0.663 ± 0.017	2.338HisGly: 2.338 ± 0.031	0.665HisHis: 0.665 ± 0.016	0.738HisIle: 0.738 ± 0.015	0.372HisLys: 0.372 ± 0.012	2.364HisLeu: 2.364 ± 0.031	0.335HisMet: 0.335 ± 0.011	0.402HisAsn: 0.402 ± 0.012	1.77HisPro: 1.77 ± 0.025	0.671HisGln: 0.671 ± 0.014	2.081HisArg: 2.081 ± 0.027	1.088HisSer: 1.088 ± 0.024	1.342HisThr: 1.342 ± 0.022	1.717HisVal: 1.717 ± 0.026	0.372HisTrp: 0.372 ± 0.011	0.499HisTyr: 0.499 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.88IleAla: 4.88 ± 0.048	0.31IleCys: 0.31 ± 0.012	2.385IleAsp: 2.385 ± 0.033	2.036IleGlu: 2.036 ± 0.03	0.806IlePhe: 0.806 ± 0.019	3.635IleGly: 3.635 ± 0.039	0.662IleHis: 0.662 ± 0.014	0.918IleIle: 0.918 ± 0.02	0.794IleLys: 0.794 ± 0.016	2.575IleLeu: 2.575 ± 0.033	0.485IleMet: 0.485 ± 0.014	0.754IleAsn: 0.754 ± 0.019	1.859IlePro: 1.859 ± 0.029	0.778IleGln: 0.778 ± 0.016	2.305IleArg: 2.305 ± 0.032	1.818IleSer: 1.818 ± 0.025	2.356IleThr: 2.356 ± 0.029	2.84IleVal: 2.84 ± 0.033	0.398IleTrp: 0.398 ± 0.013	0.568IleTyr: 0.568 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.027LysAla: 3.027 ± 0.041	0.127LysCys: 0.127 ± 0.006	1.423LysAsp: 1.423 ± 0.03	1.298LysGlu: 1.298 ± 0.024	0.511LysPhe: 0.511 ± 0.015	1.934LysGly: 1.934 ± 0.033	0.447LysHis: 0.447 ± 0.013	0.913LysIle: 0.913 ± 0.018	0.952LysLys: 0.952 ± 0.027	2.119LysLeu: 2.119 ± 0.033	0.407LysMet: 0.407 ± 0.011	0.588LysAsn: 0.588 ± 0.015	1.481LysPro: 1.481 ± 0.023	0.748LysGln: 0.748 ± 0.02	1.486LysArg: 1.486 ± 0.026	1.331LysSer: 1.331 ± 0.024	1.384LysThr: 1.384 ± 0.024	2.005LysVal: 2.005 ± 0.029	0.322LysTrp: 0.322 ± 0.012	0.508LysTyr: 0.508 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.562LeuAla: 14.562 ± 0.094	0.836LeuCys: 0.836 ± 0.017	6.658LeuAsp: 6.658 ± 0.051	4.735LeuGlu: 4.735 ± 0.047	2.682LeuPhe: 2.682 ± 0.037	9.167LeuGly: 9.167 ± 0.06	2.266LeuHis: 2.266 ± 0.027	3.45LeuIle: 3.45 ± 0.044	2.171LeuLys: 2.171 ± 0.03	11.157LeuLeu: 11.157 ± 0.083	1.697LeuMet: 1.697 ± 0.019	1.75LeuAsn: 1.75 ± 0.029	6.368LeuPro: 6.368 ± 0.053	2.204LeuGln: 2.204 ± 0.028	8.568LeuArg: 8.568 ± 0.063	5.56LeuSer: 5.56 ± 0.049	6.807LeuThr: 6.807 ± 0.041	8.699LeuVal: 8.699 ± 0.062	1.335LeuTrp: 1.335 ± 0.026	1.88LeuTyr: 1.88 ± 0.027	0.001LeuXaa: 0.001 ± 0.001
Met	2.343MetAla: 2.343 ± 0.029	0.132MetCys: 0.132 ± 0.007	0.94MetAsp: 0.94 ± 0.023	0.778MetGlu: 0.778 ± 0.016	0.463MetPhe: 0.463 ± 0.012	1.371MetGly: 1.371 ± 0.024	0.357MetHis: 0.357 ± 0.01	0.678MetIle: 0.678 ± 0.017	0.447MetLys: 0.447 ± 0.014	1.741MetLeu: 1.741 ± 0.025	0.312MetMet: 0.312 ± 0.011	0.448MetAsn: 0.448 ± 0.013	1.129MetPro: 1.129 ± 0.023	0.441MetGln: 0.441 ± 0.013	1.454MetArg: 1.454 ± 0.023	1.376MetSer: 1.376 ± 0.022	1.555MetThr: 1.555 ± 0.023	1.347MetVal: 1.347 ± 0.023	0.224MetTrp: 0.224 ± 0.007	0.34MetTyr: 0.34 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.246AsnAla: 2.246 ± 0.033	0.17AsnCys: 0.17 ± 0.009	0.999AsnAsp: 0.999 ± 0.017	0.898AsnGlu: 0.898 ± 0.021	0.551AsnPhe: 0.551 ± 0.015	2.063AsnGly: 2.063 ± 0.032	0.411AsnHis: 0.411 ± 0.013	0.718AsnIle: 0.718 ± 0.016	0.46AsnLys: 0.46 ± 0.014	1.691AsnLeu: 1.691 ± 0.026	0.302AsnMet: 0.302 ± 0.009	0.463AsnAsn: 0.463 ± 0.016	1.419AsnPro: 1.419 ± 0.024	0.558AsnGln: 0.558 ± 0.013	1.261AsnArg: 1.261 ± 0.024	1.073AsnSer: 1.073 ± 0.024	1.179AsnThr: 1.179 ± 0.021	1.452AsnVal: 1.452 ± 0.027	0.324AsnTrp: 0.324 ± 0.012	0.461AsnTyr: 0.461 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	7.746ProAla: 7.746 ± 0.062	0.331ProCys: 0.331 ± 0.012	4.467ProAsp: 4.467 ± 0.039	4.379ProGlu: 4.379 ± 0.045	1.528ProPhe: 1.528 ± 0.02	6.342ProGly: 6.342 ± 0.061	1.39ProHis: 1.39 ± 0.024	1.355ProIle: 1.355 ± 0.024	1.306ProLys: 1.306 ± 0.022	5.372ProLeu: 5.372 ± 0.045	1.03ProMet: 1.03 ± 0.017	0.976ProAsn: 0.976 ± 0.019	3.327ProPro: 3.327 ± 0.052	1.825ProGln: 1.825 ± 0.027	3.832ProArg: 3.832 ± 0.043	3.375ProSer: 3.375 ± 0.036	3.196ProThr: 3.196 ± 0.038	5.311ProVal: 5.311 ± 0.048	0.937ProTrp: 0.937 ± 0.018	1.452ProTyr: 1.452 ± 0.023	0.001ProXaa: 0.001 ± 0.0
Gln	3.777GlnAla: 3.777 ± 0.043	0.173GlnCys: 0.173 ± 0.008	1.495GlnAsp: 1.495 ± 0.026	1.484GlnGlu: 1.484 ± 0.023	0.71GlnPhe: 0.71 ± 0.017	2.447GlnGly: 2.447 ± 0.029	0.65GlnHis: 0.65 ± 0.016	1.094GlnIle: 1.094 ± 0.02	0.656GlnLys: 0.656 ± 0.018	3.119GlnLeu: 3.119 ± 0.035	0.511GlnMet: 0.511 ± 0.013	0.56GlnAsn: 0.56 ± 0.014	1.736GlnPro: 1.736 ± 0.033	1.3GlnGln: 1.3 ± 0.028	2.406GlnArg: 2.406 ± 0.031	1.376GlnSer: 1.376 ± 0.021	1.39GlnThr: 1.39 ± 0.024	2.388GlnVal: 2.388 ± 0.033	0.491GlnTrp: 0.491 ± 0.013	0.639GlnTyr: 0.639 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.359ArgAla: 9.359 ± 0.066	0.602ArgCys: 0.602 ± 0.014	4.242ArgAsp: 4.242 ± 0.042	4.672ArgGlu: 4.672 ± 0.045	2.326ArgPhe: 2.326 ± 0.029	5.775ArgGly: 5.775 ± 0.05	2.102ArgHis: 2.102 ± 0.028	3.278ArgIle: 3.278 ± 0.037	1.707ArgLys: 1.707 ± 0.025	8.637ArgLeu: 8.637 ± 0.074	1.713ArgMet: 1.713 ± 0.022	1.39ArgAsn: 1.39 ± 0.026	4.729ArgPro: 4.729 ± 0.041	2.397ArgGln: 2.397 ± 0.029	7.49ArgArg: 7.49 ± 0.066	4.067ArgSer: 4.067 ± 0.043	5.298ArgThr: 5.298 ± 0.044	5.715ArgVal: 5.715 ± 0.044	1.356ArgTrp: 1.356 ± 0.022	1.799ArgTyr: 1.799 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.881SerAla: 6.881 ± 0.048	0.426SerCys: 0.426 ± 0.014	2.91SerAsp: 2.91 ± 0.037	2.658SerGlu: 2.658 ± 0.034	1.603SerPhe: 1.603 ± 0.022	6.249SerGly: 6.249 ± 0.055	1.076SerHis: 1.076 ± 0.021	1.556SerIle: 1.556 ± 0.023	1.177SerLys: 1.177 ± 0.024	5.041SerLeu: 5.041 ± 0.045	1.137SerMet: 1.137 ± 0.021	0.972SerAsn: 0.972 ± 0.021	3.33SerPro: 3.33 ± 0.037	1.39SerGln: 1.39 ± 0.024	3.812SerArg: 3.812 ± 0.04	3.266SerSer: 3.266 ± 0.044	3.241SerThr: 3.241 ± 0.035	4.443SerVal: 4.443 ± 0.041	0.967SerTrp: 0.967 ± 0.019	1.266SerTyr: 1.266 ± 0.021	0.001SerXaa: 0.001 ± 0.0
Thr	8.625ThrAla: 8.625 ± 0.067	0.437ThrCys: 0.437 ± 0.014	3.585ThrAsp: 3.585 ± 0.032	3.389ThrGlu: 3.389 ± 0.032	1.693ThrPhe: 1.693 ± 0.024	6.615ThrGly: 6.615 ± 0.052	1.222ThrHis: 1.222 ± 0.022	1.792ThrIle: 1.792 ± 0.029	1.316ThrLys: 1.316 ± 0.023	5.736ThrLeu: 5.736 ± 0.049	0.987ThrMet: 0.987 ± 0.017	1.093ThrAsn: 1.093 ± 0.022	4.072ThrPro: 4.072 ± 0.045	1.364ThrGln: 1.364 ± 0.023	3.849ThrArg: 3.849 ± 0.039	3.378ThrSer: 3.378 ± 0.042	3.888ThrThr: 3.888 ± 0.046	6.047ThrVal: 6.047 ± 0.044	0.936ThrTrp: 0.936 ± 0.023	1.449ThrTyr: 1.449 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	10.399ValAla: 10.399 ± 0.072	0.758ValCys: 0.758 ± 0.017	4.986ValAsp: 4.986 ± 0.043	4.742ValGlu: 4.742 ± 0.04	2.528ValPhe: 2.528 ± 0.034	6.524ValGly: 6.524 ± 0.058	1.898ValHis: 1.898 ± 0.024	3.052ValIle: 3.052 ± 0.038	1.783ValLys: 1.783 ± 0.029	9.393ValLeu: 9.393 ± 0.07	1.481ValMet: 1.481 ± 0.026	1.758ValAsn: 1.758 ± 0.029	5.083ValPro: 5.083 ± 0.04	2.106ValGln: 2.106 ± 0.029	7.034ValArg: 7.034 ± 0.058	4.557ValSer: 4.557 ± 0.04	5.565ValThr: 5.565 ± 0.054	7.929ValVal: 7.929 ± 0.071	1.159ValTrp: 1.159 ± 0.022	1.604ValTyr: 1.604 ± 0.026	0.002ValXaa: 0.002 ± 0.001
Trp	1.656TrpAla: 1.656 ± 0.027	0.145TrpCys: 0.145 ± 0.008	0.875TrpAsp: 0.875 ± 0.018	0.743TrpGlu: 0.743 ± 0.017	0.52TrpPhe: 0.52 ± 0.012	1.139TrpGly: 1.139 ± 0.021	0.376TrpHis: 0.376 ± 0.011	0.572TrpIle: 0.572 ± 0.014	0.394TrpLys: 0.394 ± 0.012	1.798TrpLeu: 1.798 ± 0.025	0.31TrpMet: 0.31 ± 0.01	0.445TrpAsn: 0.445 ± 0.014	0.803TrpPro: 0.803 ± 0.017	0.64TrpGln: 0.64 ± 0.016	1.35TrpArg: 1.35 ± 0.023	0.939TrpSer: 0.939 ± 0.018	1.052TrpThr: 1.052 ± 0.021	1.045TrpVal: 1.045 ± 0.021	0.34TrpTrp: 0.34 ± 0.012	0.394TrpTyr: 0.394 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.798TyrAla: 2.798 ± 0.028	0.182TyrCys: 0.182 ± 0.007	1.537TyrAsp: 1.537 ± 0.026	1.381TyrGlu: 1.381 ± 0.023	0.673TyrPhe: 0.673 ± 0.018	2.371TyrGly: 2.371 ± 0.032	0.392TyrHis: 0.392 ± 0.013	0.537TyrIle: 0.537 ± 0.014	0.441TyrLys: 0.441 ± 0.014	2.096TyrLeu: 2.096 ± 0.025	0.279TyrMet: 0.279 ± 0.009	0.455TyrAsn: 0.455 ± 0.015	1.079TyrPro: 1.079 ± 0.022	0.627TyrGln: 0.627 ± 0.015	1.785TyrArg: 1.785 ± 0.028	1.003TyrSer: 1.003 ± 0.023	1.23TyrThr: 1.23 ± 0.022	1.719TyrVal: 1.719 ± 0.022	0.377TyrTrp: 0.377 ± 0.013	0.5TyrTyr: 0.5 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8775 proteins (2919780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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