Tuwongella immobilis
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5223 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C2YUW9|A0A6C2YUW9_9BACT Chromosome partition protein Smc OS=Tuwongella immobilis OX=692036 GN=smc PE=3 SV=1
MM1 pKa = 7.55 ASDD4 pKa = 4.15 VIGPTVVGVVDD15 pKa = 4.08 VDD17 pKa = 3.54 NHH19 pKa = 6.18 PLPIRR24 pKa = 11.84 CLGEE28 pKa = 3.61 NSFLVDD34 pKa = 3.23 PVVAVLWLHH43 pKa = 5.82 QPQPGCGLAVGCVEE57 pKa = 4.07 AVEE60 pKa = 4.54 MPLAVALDD68 pKa = 3.84 GGGGDD73 pKa = 3.97 GSQIALAEE81 pKa = 4.04 FAVDD85 pKa = 3.49 LFQAFRR91 pKa = 11.84 GVV93 pKa = 3.0
Molecular weight: 9.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.91
IPC_protein 3.808
Toseland 3.617
ProMoST 4.012
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A6C2YVK1|A0A6C2YVK1_9BACT RCK N-terminal domain-containing protein OS=Tuwongella immobilis OX=692036 GN=GMBLW1_41840 PE=4 SV=1
MM1 pKa = 7.6 AKK3 pKa = 10.1 KK4 pKa = 10.49 NKK6 pKa = 7.96 TNRR9 pKa = 11.84 SLKK12 pKa = 9.81 KK13 pKa = 9.89 RR14 pKa = 11.84 FRR16 pKa = 11.84 ITATGKK22 pKa = 9.88 IKK24 pKa = 10.38 HH25 pKa = 5.14 GHH27 pKa = 4.15 VNRR30 pKa = 11.84 RR31 pKa = 11.84 HH32 pKa = 5.54 LLAGRR37 pKa = 11.84 TSKK40 pKa = 10.65 RR41 pKa = 11.84 KK42 pKa = 9.25 RR43 pKa = 11.84 QLGQPAVRR51 pKa = 11.84 NDD53 pKa = 2.85 IVAKK57 pKa = 10.39 KK58 pKa = 9.84 IVLAMGGAA66 pKa = 3.63
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.385
IPC2_protein 10.847
IPC_protein 12.384
Toseland 12.574
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.457
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.179
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.007
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5223
0
5223
1989792
29
10565
381.0
41.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.894 ± 0.04
0.956 ± 0.015
5.449 ± 0.026
5.624 ± 0.045
3.592 ± 0.018
7.884 ± 0.073
2.03 ± 0.023
5.195 ± 0.026
3.37 ± 0.044
10.317 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.291 ± 0.024
3.179 ± 0.036
6.364 ± 0.039
4.197 ± 0.031
7.083 ± 0.05
6.064 ± 0.04
5.864 ± 0.06
6.813 ± 0.038
1.637 ± 0.02
2.197 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here