Dishui Lake phycodnavirus 4

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; unclassified Phycodnaviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XP25|A0A6G6XP25_9PHYC Uncharacterized protein OS=Dishui Lake phycodnavirus 4 OX=2704072 PE=4 SV=1
MM1 pKa = 7.44IVLILLLVLLIAAGIGTYY19 pKa = 10.14FIVNKK24 pKa = 10.25NATDD28 pKa = 3.44TNTADD33 pKa = 3.61TAAGAAAGAVEE44 pKa = 4.49DD45 pKa = 4.72EE46 pKa = 4.55EE47 pKa = 4.72DD48 pKa = 4.63AEE50 pKa = 4.75DD51 pKa = 3.76EE52 pKa = 4.24EE53 pKa = 4.66EE54 pKa = 3.94AAKK57 pKa = 10.42AAEE60 pKa = 4.28AAAGAAGAAAAGAAGAAGAAAGEE83 pKa = 4.44TATTLAGLQEE93 pKa = 5.25AIGTPPPVDD102 pKa = 4.07PPVSDD107 pKa = 4.87PDD109 pKa = 4.45DD110 pKa = 4.72PDD112 pKa = 3.66AAASAASGVTQQQIATPPGSDD133 pKa = 3.38PVIDD137 pKa = 4.02CVGSFGSFSTCSVGCGGGTKK157 pKa = 10.08SRR159 pKa = 11.84TYY161 pKa = 10.59TVTTPASGGGQACTRR176 pKa = 11.84EE177 pKa = 4.07NGYY180 pKa = 10.66VDD182 pKa = 4.12TVPCNTDD189 pKa = 3.35PCPVDD194 pKa = 6.15CIGEE198 pKa = 4.14WNVWSTCSQTCGGGLRR214 pKa = 11.84KK215 pKa = 9.55RR216 pKa = 11.84SYY218 pKa = 10.62NYY220 pKa = 10.15SRR222 pKa = 11.84PAEE225 pKa = 4.27HH226 pKa = 7.14GGQEE230 pKa = 4.19CKK232 pKa = 10.5VDD234 pKa = 3.72GEE236 pKa = 4.37LMANGYY242 pKa = 8.94EE243 pKa = 3.98EE244 pKa = 4.77SEE246 pKa = 4.64GVIHH250 pKa = 6.96PCPIHH255 pKa = 7.27CAGTWNEE262 pKa = 3.79

Molecular weight: 26.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XP68|A0A6G6XP68_9PHYC Uncharacterized protein OS=Dishui Lake phycodnavirus 4 OX=2704072 PE=3 SV=1
MM1 pKa = 7.73PLSKK5 pKa = 10.67YY6 pKa = 10.13GYY8 pKa = 10.12HH9 pKa = 7.75DD10 pKa = 3.47IKK12 pKa = 11.08QKK14 pKa = 10.01TEE16 pKa = 4.01LARR19 pKa = 11.84HH20 pKa = 5.59RR21 pKa = 11.84ALLKK25 pKa = 10.34ALRR28 pKa = 11.84EE29 pKa = 4.33GEE31 pKa = 3.92SGIRR35 pKa = 11.84IFRR38 pKa = 11.84KK39 pKa = 9.43LARR42 pKa = 11.84LSTIYY47 pKa = 10.24KK48 pKa = 10.18YY49 pKa = 10.74KK50 pKa = 10.56DD51 pKa = 3.2PKK53 pKa = 10.89LSTKK57 pKa = 10.32FKK59 pKa = 10.59RR60 pKa = 11.84DD61 pKa = 3.35RR62 pKa = 11.84DD63 pKa = 3.47WMRR66 pKa = 11.84KK67 pKa = 9.32HH68 pKa = 6.1II69 pKa = 4.38

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

54299

54

9528

315.7

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.82 ± 1.157

1.44 ± 0.275

5.057 ± 0.492

5.676 ± 0.592

4.252 ± 0.269

7.418 ± 1.235

1.847 ± 0.208

7.265 ± 0.19

6.455 ± 0.97

7.256 ± 0.494

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.223

6.639 ± 0.443

3.604 ± 0.507

4.4 ± 0.48

4.098 ± 0.355

7.571 ± 0.799

6.138 ± 0.197

6.315 ± 0.159

0.777 ± 0.125

4.337 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski