Truepera radiovictrix (strain DSM 17093 / CIP 108686 / LMG 22925 / RQ-24)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2924 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D7CQH3|D7CQH3_TRURR Oxidoreductase domain protein OS=Truepera radiovictrix (strain DSM 17093 / CIP 108686 / LMG 22925 / RQ-24) OX=649638 GN=Trad_1841 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.08 RR3 pKa = 11.84 TLATATLLAGTLLGAASAQDD23 pKa = 3.74 APSGAITVTAWDD35 pKa = 3.46 IAAEE39 pKa = 4.0 ALEE42 pKa = 3.99 ALVPGFNEE50 pKa = 3.92 QYY52 pKa = 10.33 PDD54 pKa = 3.49 VQVTVEE60 pKa = 4.04 NLGNQQVFDD69 pKa = 4.52 RR70 pKa = 11.84 GLAGCAAGGADD81 pKa = 4.55 LPDD84 pKa = 3.63 VYY86 pKa = 11.15 AVEE89 pKa = 4.21 NNEE92 pKa = 5.75 AEE94 pKa = 4.59 VFWNRR99 pKa = 11.84 FPDD102 pKa = 3.85 CFLDD106 pKa = 4.08 LTALGADD113 pKa = 4.21 EE114 pKa = 5.64 LRR116 pKa = 11.84 DD117 pKa = 3.98 DD118 pKa = 4.93 FPDD121 pKa = 4.55 FKK123 pKa = 10.04 WTEE126 pKa = 3.91 LTVGEE131 pKa = 4.33 RR132 pKa = 11.84 VYY134 pKa = 10.89 AIPWDD139 pKa = 3.78 SGPVVTFYY147 pKa = 10.99 RR148 pKa = 11.84 RR149 pKa = 11.84 DD150 pKa = 3.39 LYY152 pKa = 11.14 EE153 pKa = 3.95 EE154 pKa = 4.86 AGINPDD160 pKa = 5.29 EE161 pKa = 4.93 IEE163 pKa = 3.96 TWDD166 pKa = 3.89 DD167 pKa = 4.0 FIEE170 pKa = 4.71 AGQALDD176 pKa = 3.59 EE177 pKa = 4.32 ATGAKK182 pKa = 9.09 MLWFDD187 pKa = 4.85 FSDD190 pKa = 3.88 DD191 pKa = 4.04 GVWRR195 pKa = 11.84 QLANQNGCFYY205 pKa = 10.57 FDD207 pKa = 3.82 PEE209 pKa = 4.38 GTEE212 pKa = 3.87 VTVNQPGCVDD222 pKa = 2.97 AMEE225 pKa = 4.43 TMKK228 pKa = 10.97 ALYY231 pKa = 10.03 DD232 pKa = 3.69 AGIVAIVDD240 pKa = 3.31 WNGAIQEE247 pKa = 4.23 IRR249 pKa = 11.84 NGTLAGAVFGAWYY262 pKa = 10.47 VGTIQSNAPDD272 pKa = 3.51 QEE274 pKa = 4.84 GNWGVYY280 pKa = 8.3 EE281 pKa = 4.39 MPASEE286 pKa = 4.31 PGGVRR291 pKa = 11.84 AANLGGSALAIPASSQNPEE310 pKa = 3.26 AAMAFIRR317 pKa = 11.84 YY318 pKa = 8.76 ALATTEE324 pKa = 4.55 GQVSMLRR331 pKa = 11.84 NQGLVPSYY339 pKa = 10.89 LPALEE344 pKa = 4.7 DD345 pKa = 4.18 PYY347 pKa = 11.12 VQEE350 pKa = 4.49 PVPYY354 pKa = 10.01 WGDD357 pKa = 3.33 QPIWQDD363 pKa = 2.79 ILATLGEE370 pKa = 4.31 IPQARR375 pKa = 11.84 GTQYY379 pKa = 10.35 FQEE382 pKa = 4.11 ARR384 pKa = 11.84 QIVTNTLSQYY394 pKa = 8.32 LTQNAYY400 pKa = 10.13 EE401 pKa = 4.42 DD402 pKa = 3.88 AQAAMDD408 pKa = 4.99 DD409 pKa = 3.68 AAQQISAAVGLPIAGQQ425 pKa = 3.22
Molecular weight: 45.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.834
Patrickios 1.252
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|D7CTU7|D7CTU7_TRURR Uncharacterized protein OS=Truepera radiovictrix (strain DSM 17093 / CIP 108686 / LMG 22925 / RQ-24) OX=649638 GN=Trad_2538 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.5 LNRR5 pKa = 11.84 FLRR8 pKa = 11.84 TALKK12 pKa = 10.76 LFVLTRR18 pKa = 11.84 GFRR21 pKa = 11.84 AARR24 pKa = 11.84 RR25 pKa = 11.84 GVGRR29 pKa = 11.84 YY30 pKa = 8.31 GGRR33 pKa = 11.84 RR34 pKa = 11.84 VRR36 pKa = 11.84 GGVVQQLMRR45 pKa = 11.84 RR46 pKa = 11.84 LLRR49 pKa = 4.36
Molecular weight: 5.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.458
IPC2_protein 11.169
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.31
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.047
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2924
0
2924
943825
35
3080
322.8
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.344 ± 0.062
0.652 ± 0.012
4.592 ± 0.039
6.623 ± 0.043
3.696 ± 0.028
8.829 ± 0.041
2.02 ± 0.024
3.182 ± 0.041
2.151 ± 0.035
12.421 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.609 ± 0.018
2.027 ± 0.024
5.859 ± 0.035
2.904 ± 0.022
8.02 ± 0.047
4.678 ± 0.028
5.402 ± 0.031
8.107 ± 0.039
1.355 ± 0.018
2.528 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here