Nanosalina sp. (strain J07AB43)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Nanohaloarchaea; Candidatus Nanosalina; unclassified Candidatus Nanosalina

Average proteome isoelectric point is 5.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0QI38|G0QI38_NANS0 Uncharacterized protein (Fragment) OS=Nanosalina sp. (strain J07AB43) OX=889948 GN=J07AB43_09070 PE=4 SV=1
VV1 pKa = 6.76FVVDD5 pKa = 4.06DD6 pKa = 4.89HH7 pKa = 6.45NHH9 pKa = 6.44ALAGWTAALYY19 pKa = 10.05EE20 pKa = 4.34GLFDD24 pKa = 3.93SRR26 pKa = 11.84PILVHH31 pKa = 6.48VDD33 pKa = 3.34YY34 pKa = 11.34HH35 pKa = 6.86EE36 pKa = 6.12DD37 pKa = 3.38SANPPEE43 pKa = 4.43VFNTNLPTDD52 pKa = 4.45FPTLEE57 pKa = 4.4DD58 pKa = 3.39QVHH61 pKa = 6.25LLEE64 pKa = 5.77IDD66 pKa = 3.57EE67 pKa = 5.22FIEE70 pKa = 4.68AGKK73 pKa = 8.55MWDD76 pKa = 3.82IYY78 pKa = 11.56DD79 pKa = 3.53EE80 pKa = 4.51VINVGVQSYY89 pKa = 10.81YY90 pKa = 11.22SDD92 pKa = 4.56LDD94 pKa = 3.31QDD96 pKa = 4.36LYY98 pKa = 11.55RR99 pKa = 11.84MKK101 pKa = 10.8EE102 pKa = 3.85AMQDD106 pKa = 3.33SDD108 pKa = 5.98DD109 pKa = 4.46VILDD113 pKa = 2.91IDD115 pKa = 3.81MYY117 pKa = 11.31VYY119 pKa = 10.95NRR121 pKa = 11.84DD122 pKa = 3.86DD123 pKa = 4.21LVDD126 pKa = 4.91DD127 pKa = 4.41FDD129 pKa = 5.88LRR131 pKa = 11.84LADD134 pKa = 4.06AVSEE138 pKa = 4.57SEE140 pKa = 4.19FTSFATSPGYY150 pKa = 10.58VQDD153 pKa = 3.55QEE155 pKa = 4.73EE156 pKa = 4.73IIEE159 pKa = 4.47KK160 pKa = 10.39INGIVEE166 pKa = 4.02MADD169 pKa = 3.4RR170 pKa = 11.84LL171 pKa = 3.88

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0QHM9|G0QHM9_NANS0 Mn-dependent transcriptional regulator OS=Nanosalina sp. (strain J07AB43) OX=889948 GN=J07AB43_07470 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 4.87SFCFFTEE9 pKa = 3.83KK10 pKa = 10.34MKK12 pKa = 10.22DD13 pKa = 2.82TGRR16 pKa = 11.84YY17 pKa = 8.71LVAKK21 pKa = 9.52LRR23 pKa = 11.84KK24 pKa = 8.95RR25 pKa = 11.84SRR27 pKa = 11.84LHH29 pKa = 5.32RR30 pKa = 11.84QLYY33 pKa = 7.67VKK35 pKa = 9.88PVKK38 pKa = 10.41RR39 pKa = 3.78

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1673

0

1673

354589

13

1924

211.9

23.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.905 ± 0.057

0.673 ± 0.021

7.402 ± 0.064

10.251 ± 0.119

3.859 ± 0.042

6.922 ± 0.07

1.699 ± 0.036

6.232 ± 0.057

5.825 ± 0.075

8.211 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.029

4.623 ± 0.054

3.437 ± 0.04

3.755 ± 0.049

4.838 ± 0.049

7.554 ± 0.082

5.233 ± 0.052

6.897 ± 0.061

0.858 ± 0.021

3.257 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski