Methylophaga muralis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Methylophaga

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2793 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3GP42|A0A1E3GP42_9GAMM All-trans-phytoene synthase OS=Methylophaga muralis OX=291169 GN=crtB_2 PE=4 SV=1
MM1 pKa = 7.07TFVVTEE7 pKa = 3.83NCIKK11 pKa = 10.53CKK13 pKa = 10.04YY14 pKa = 7.62TDD16 pKa = 4.08CVDD19 pKa = 3.61VCPVDD24 pKa = 4.48CFHH27 pKa = 7.43EE28 pKa = 5.34GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.64EE39 pKa = 6.0CIDD42 pKa = 3.79CTLCEE47 pKa = 4.33PEE49 pKa = 5.12CPAEE53 pKa = 4.58AIFSEE58 pKa = 5.02DD59 pKa = 4.26DD60 pKa = 3.82LPDD63 pKa = 3.34EE64 pKa = 4.5QMEE67 pKa = 4.12FLQINAEE74 pKa = 4.27LSQVWPVLSEE84 pKa = 4.24KK85 pKa = 10.49KK86 pKa = 10.53DD87 pKa = 3.7PLDD90 pKa = 5.45DD91 pKa = 4.29AEE93 pKa = 4.45EE94 pKa = 4.21WDD96 pKa = 3.94GKK98 pKa = 9.17PNKK101 pKa = 9.55TPLLEE106 pKa = 4.11RR107 pKa = 4.73

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3GPJ4|A0A1E3GPJ4_9GAMM Uncharacterized protein OS=Methylophaga muralis OX=291169 GN=A9E74_02318 PE=4 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84FFRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.28FCRR12 pKa = 11.84FTAEE16 pKa = 3.95GVKK19 pKa = 10.44QIDD22 pKa = 3.92YY23 pKa = 11.16KK24 pKa = 11.27DD25 pKa = 3.6VNLLKK30 pKa = 11.06NFVTEE35 pKa = 3.93TGKK38 pKa = 10.09IVPSRR43 pKa = 11.84ITGTKK48 pKa = 9.9ARR50 pKa = 11.84YY51 pKa = 8.49QRR53 pKa = 11.84QLSTCVKK60 pKa = 9.78RR61 pKa = 11.84ARR63 pKa = 11.84FLALLPYY70 pKa = 10.47CDD72 pKa = 3.3MHH74 pKa = 7.36KK75 pKa = 11.01

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2793

0

2793

857838

29

2869

307.1

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.003 ± 0.047

0.89 ± 0.015

5.706 ± 0.033

6.183 ± 0.041

4.015 ± 0.033

6.756 ± 0.041

2.336 ± 0.022

6.593 ± 0.038

4.468 ± 0.035

10.837 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.023

4.024 ± 0.033

4.118 ± 0.033

5.117 ± 0.046

5.117 ± 0.033

6.122 ± 0.036

5.264 ± 0.03

6.638 ± 0.034

1.304 ± 0.02

2.782 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski