Amino acid dipepetide frequency for Methylophaga muralis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.63AlaAla: 8.63 ± 0.125
0.886AlaCys: 0.886 ± 0.033
5.809AlaAsp: 5.809 ± 0.086
6.909AlaGlu: 6.909 ± 0.103
3.439AlaPhe: 3.439 ± 0.071
6.473AlaGly: 6.473 ± 0.103
1.718AlaHis: 1.718 ± 0.047
6.402AlaIle: 6.402 ± 0.097
4.042AlaLys: 4.042 ± 0.069
9.862AlaLeu: 9.862 ± 0.117
2.86AlaMet: 2.86 ± 0.063
3.403AlaAsn: 3.403 ± 0.067
2.821AlaPro: 2.821 ± 0.059
3.611AlaGln: 3.611 ± 0.065
4.236AlaArg: 4.236 ± 0.075
4.958AlaSer: 4.958 ± 0.076
4.406AlaThr: 4.406 ± 0.078
6.252AlaVal: 6.252 ± 0.1
1.025AlaTrp: 1.025 ± 0.039
2.286AlaTyr: 2.286 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.699CysAla: 0.699 ± 0.028
0.138CysCys: 0.138 ± 0.012
0.553CysAsp: 0.553 ± 0.027
0.568CysGlu: 0.568 ± 0.028
0.337CysPhe: 0.337 ± 0.021
0.835CysGly: 0.835 ± 0.037
0.322CysHis: 0.322 ± 0.021
0.501CysIle: 0.501 ± 0.023
0.322CysLys: 0.322 ± 0.018
0.913CysLeu: 0.913 ± 0.036
0.209CysMet: 0.209 ± 0.017
0.29CysAsn: 0.29 ± 0.019
0.484CysPro: 0.484 ± 0.026
0.431CysGln: 0.431 ± 0.021
0.477CysArg: 0.477 ± 0.023
0.514CysSer: 0.514 ± 0.028
0.364CysThr: 0.364 ± 0.022
0.557CysVal: 0.557 ± 0.028
0.124CysTrp: 0.124 ± 0.012
0.267CysTyr: 0.267 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.791AspAla: 4.791 ± 0.07
0.451AspCys: 0.451 ± 0.025
3.311AspAsp: 3.311 ± 0.076
4.059AspGlu: 4.059 ± 0.082
2.512AspPhe: 2.512 ± 0.058
3.857AspGly: 3.857 ± 0.08
1.159AspHis: 1.159 ± 0.041
4.183AspIle: 4.183 ± 0.067
3.038AspLys: 3.038 ± 0.067
5.848AspLeu: 5.848 ± 0.089
1.462AspMet: 1.462 ± 0.043
2.418AspAsn: 2.418 ± 0.056
2.399AspPro: 2.399 ± 0.052
2.249AspGln: 2.249 ± 0.049
2.715AspArg: 2.715 ± 0.057
3.327AspSer: 3.327 ± 0.068
2.612AspThr: 2.612 ± 0.056
3.789AspVal: 3.789 ± 0.069
0.952AspTrp: 0.952 ± 0.04
1.932AspTyr: 1.932 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.669GluAla: 5.669 ± 0.099
0.441GluCys: 0.441 ± 0.025
2.933GluAsp: 2.933 ± 0.061
3.209GluGlu: 3.209 ± 0.087
2.376GluPhe: 2.376 ± 0.052
3.383GluGly: 3.383 ± 0.071
1.478GluHis: 1.478 ± 0.041
4.236GluIle: 4.236 ± 0.08
3.398GluLys: 3.398 ± 0.057
6.576GluLeu: 6.576 ± 0.093
1.887GluMet: 1.887 ± 0.047
2.83GluAsn: 2.83 ± 0.059
2.182GluPro: 2.182 ± 0.06
4.236GluGln: 4.236 ± 0.084
3.435GluArg: 3.435 ± 0.068
3.445GluSer: 3.445 ± 0.066
3.586GluThr: 3.586 ± 0.063
4.23GluVal: 4.23 ± 0.078
0.771GluTrp: 0.771 ± 0.03
1.531GluTyr: 1.531 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.665PheAla: 3.665 ± 0.071
0.452PheCys: 0.452 ± 0.021
2.653PheAsp: 2.653 ± 0.057
2.462PheGlu: 2.462 ± 0.058
1.738PhePhe: 1.738 ± 0.056
2.991PheGly: 2.991 ± 0.066
0.878PheHis: 0.878 ± 0.031
2.792PheIle: 2.792 ± 0.063
1.598PheLys: 1.598 ± 0.045
3.599PheLeu: 3.599 ± 0.075
1.044PheMet: 1.044 ± 0.034
1.856PheAsn: 1.856 ± 0.05
1.491PhePro: 1.491 ± 0.04
1.357PheGln: 1.357 ± 0.043
1.823PheArg: 1.823 ± 0.043
3.067PheSer: 3.067 ± 0.067
2.216PheThr: 2.216 ± 0.052
2.593PheVal: 2.593 ± 0.055
0.525PheTrp: 0.525 ± 0.029
1.35PheTyr: 1.35 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.076GlyAla: 5.076 ± 0.098
0.768GlyCys: 0.768 ± 0.033
3.659GlyAsp: 3.659 ± 0.071
4.476GlyGlu: 4.476 ± 0.077
3.196GlyPhe: 3.196 ± 0.072
4.765GlyGly: 4.765 ± 0.103
1.667GlyHis: 1.667 ± 0.051
4.747GlyIle: 4.747 ± 0.085
3.609GlyLys: 3.609 ± 0.078
7.437GlyLeu: 7.437 ± 0.097
2.076GlyMet: 2.076 ± 0.049
2.478GlyAsn: 2.478 ± 0.066
1.888GlyPro: 1.888 ± 0.049
2.977GlyGln: 2.977 ± 0.054
3.555GlyArg: 3.555 ± 0.073
3.832GlySer: 3.832 ± 0.065
3.17GlyThr: 3.17 ± 0.067
4.756GlyVal: 4.756 ± 0.085
1.041GlyTrp: 1.041 ± 0.038
2.389GlyTyr: 2.389 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.876HisAla: 1.876 ± 0.055
0.319HisCys: 0.319 ± 0.017
1.288HisAsp: 1.288 ± 0.044
1.223HisGlu: 1.223 ± 0.039
1.047HisPhe: 1.047 ± 0.036
1.666HisGly: 1.666 ± 0.045
0.802HisHis: 0.802 ± 0.032
1.552HisIle: 1.552 ± 0.049
0.952HisLys: 0.952 ± 0.033
2.333HisLeu: 2.333 ± 0.048
0.53HisMet: 0.53 ± 0.026
0.906HisAsn: 0.906 ± 0.037
1.342HisPro: 1.342 ± 0.04
1.324HisGln: 1.324 ± 0.047
1.194HisArg: 1.194 ± 0.035
1.367HisSer: 1.367 ± 0.042
1.086HisThr: 1.086 ± 0.036
1.282HisVal: 1.282 ± 0.04
0.398HisTrp: 0.398 ± 0.022
0.874HisTyr: 0.874 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.586IleAla: 6.586 ± 0.096
0.636IleCys: 0.636 ± 0.031
4.532IleAsp: 4.532 ± 0.077
4.755IleGlu: 4.755 ± 0.083
2.19IlePhe: 2.19 ± 0.059
4.795IleGly: 4.795 ± 0.085
1.412IleHis: 1.412 ± 0.04
3.983IleIle: 3.983 ± 0.077
3.01IleLys: 3.01 ± 0.062
5.672IleLeu: 5.672 ± 0.101
1.378IleMet: 1.378 ± 0.039
3.136IleAsn: 3.136 ± 0.072
2.802IlePro: 2.802 ± 0.058
2.555IleGln: 2.555 ± 0.059
3.342IleArg: 3.342 ± 0.068
4.655IleSer: 4.655 ± 0.069
3.793IleThr: 3.793 ± 0.075
4.123IleVal: 4.123 ± 0.077
0.781IleTrp: 0.781 ± 0.032
1.788IleTyr: 1.788 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.15LysAla: 4.15 ± 0.08
0.263LysCys: 0.263 ± 0.018
2.203LysAsp: 2.203 ± 0.05
2.048LysGlu: 2.048 ± 0.049
1.331LysPhe: 1.331 ± 0.044
2.591LysGly: 2.591 ± 0.072
1.17LysHis: 1.17 ± 0.038
2.679LysIle: 2.679 ± 0.056
2.302LysLys: 2.302 ± 0.056
5.132LysLeu: 5.132 ± 0.078
1.275LysMet: 1.275 ± 0.041
1.848LysAsn: 1.848 ± 0.049
2.267LysPro: 2.267 ± 0.052
3.073LysGln: 3.073 ± 0.065
2.566LysArg: 2.566 ± 0.053
2.555LysSer: 2.555 ± 0.056
2.772LysThr: 2.772 ± 0.059
2.947LysVal: 2.947 ± 0.068
0.522LysTrp: 0.522 ± 0.028
0.987LysTyr: 0.987 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
10.481LeuAla: 10.481 ± 0.113
0.951LeuCys: 0.951 ± 0.036
5.823LeuAsp: 5.823 ± 0.085
5.984LeuGlu: 5.984 ± 0.082
4.35LeuPhe: 4.35 ± 0.077
6.842LeuGly: 6.842 ± 0.101
2.333LeuHis: 2.333 ± 0.052
7.057LeuIle: 7.057 ± 0.111
4.952LeuLys: 4.952 ± 0.074
11.879LeuLeu: 11.879 ± 0.196
2.96LeuMet: 2.96 ± 0.065
4.582LeuAsn: 4.582 ± 0.084
5.222LeuPro: 5.222 ± 0.085
5.405LeuGln: 5.405 ± 0.095
5.287LeuArg: 5.287 ± 0.087
7.599LeuSer: 7.599 ± 0.104
6.325LeuThr: 6.325 ± 0.09
6.614LeuVal: 6.614 ± 0.097
1.222LeuTrp: 1.222 ± 0.041
2.498LeuTyr: 2.498 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.745MetAla: 2.745 ± 0.07
0.167MetCys: 0.167 ± 0.015
1.416MetAsp: 1.416 ± 0.041
1.255MetGlu: 1.255 ± 0.042
0.885MetPhe: 0.885 ± 0.032
1.782MetGly: 1.782 ± 0.051
0.629MetHis: 0.629 ± 0.028
1.658MetIle: 1.658 ± 0.052
1.271MetLys: 1.271 ± 0.043
3.083MetLeu: 3.083 ± 0.069
0.789MetMet: 0.789 ± 0.037
1.107MetAsn: 1.107 ± 0.034
1.322MetPro: 1.322 ± 0.039
1.601MetGln: 1.601 ± 0.047
1.465MetArg: 1.465 ± 0.046
1.85MetSer: 1.85 ± 0.044
1.784MetThr: 1.784 ± 0.042
1.766MetVal: 1.766 ± 0.044
0.225MetTrp: 0.225 ± 0.019
0.472MetTyr: 0.472 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.6AsnAla: 3.6 ± 0.071
0.332AsnCys: 0.332 ± 0.022
2.354AsnAsp: 2.354 ± 0.052
2.298AsnGlu: 2.298 ± 0.06
1.358AsnPhe: 1.358 ± 0.043
2.71AsnGly: 2.71 ± 0.066
0.963AsnHis: 0.963 ± 0.034
2.765AsnIle: 2.765 ± 0.057
1.7AsnLys: 1.7 ± 0.044
4.143AsnLeu: 4.143 ± 0.079
1.028AsnMet: 1.028 ± 0.034
1.773AsnAsn: 1.773 ± 0.047
2.073AsnPro: 2.073 ± 0.05
2.106AsnGln: 2.106 ± 0.055
2.123AsnArg: 2.123 ± 0.053
2.366AsnSer: 2.366 ± 0.055
2.22AsnThr: 2.22 ± 0.043
2.475AsnVal: 2.475 ± 0.05
0.652AsnTrp: 0.652 ± 0.031
1.201AsnTyr: 1.201 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
3.715ProAla: 3.715 ± 0.06
0.308ProCys: 0.308 ± 0.019
2.722ProAsp: 2.722 ± 0.057
3.547ProGlu: 3.547 ± 0.065
1.763ProPhe: 1.763 ± 0.046
2.84ProGly: 2.84 ± 0.06
0.915ProHis: 0.915 ± 0.036
2.486ProIle: 2.486 ± 0.06
1.677ProLys: 1.677 ± 0.045
4.415ProLeu: 4.415 ± 0.076
1.047ProMet: 1.047 ± 0.032
1.48ProAsn: 1.48 ± 0.043
1.384ProPro: 1.384 ± 0.05
1.909ProGln: 1.909 ± 0.051
1.651ProArg: 1.651 ± 0.04
2.127ProSer: 2.127 ± 0.052
2.078ProThr: 2.078 ± 0.051
3.42ProVal: 3.42 ± 0.068
0.521ProTrp: 0.521 ± 0.026
1.177ProTyr: 1.177 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
4.916GlnAla: 4.916 ± 0.081
0.36GlnCys: 0.36 ± 0.02
2.127GlnAsp: 2.127 ± 0.054
2.094GlnGlu: 2.094 ± 0.054
1.929GlnPhe: 1.929 ± 0.048
2.751GlnGly: 2.751 ± 0.059
1.546GlnHis: 1.546 ± 0.051
3.206GlnIle: 3.206 ± 0.069
1.986GlnLys: 1.986 ± 0.05
6.237GlnLeu: 6.237 ± 0.114
1.197GlnMet: 1.197 ± 0.042
1.883GlnAsn: 1.883 ± 0.05
2.137GlnPro: 2.137 ± 0.053
4.958GlnGln: 4.958 ± 0.123
3.163GlnArg: 3.163 ± 0.075
2.995GlnSer: 2.995 ± 0.068
2.695GlnThr: 2.695 ± 0.063
2.996GlnVal: 2.996 ± 0.061
0.726GlnTrp: 0.726 ± 0.034
1.266GlnTyr: 1.266 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
3.768ArgAla: 3.768 ± 0.068
0.443ArgCys: 0.443 ± 0.026
2.966ArgAsp: 2.966 ± 0.062
3.453ArgGlu: 3.453 ± 0.068
2.354ArgPhe: 2.354 ± 0.051
2.996ArgGly: 2.996 ± 0.063
1.39ArgHis: 1.39 ± 0.041
3.378ArgIle: 3.378 ± 0.068
2.147ArgLys: 2.147 ± 0.053
6.054ArgLeu: 6.054 ± 0.092
1.493ArgMet: 1.493 ± 0.045
1.986ArgAsn: 1.986 ± 0.044
1.873ArgPro: 1.873 ± 0.045
2.978ArgGln: 2.978 ± 0.064
2.896ArgArg: 2.896 ± 0.06
2.813ArgSer: 2.813 ± 0.062
2.118ArgThr: 2.118 ± 0.054
3.335ArgVal: 3.335 ± 0.067
0.775ArgTrp: 0.775 ± 0.03
1.951ArgTyr: 1.951 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
5.416SerAla: 5.416 ± 0.08
0.54SerCys: 0.54 ± 0.025
3.273SerAsp: 3.273 ± 0.074
3.749SerGlu: 3.749 ± 0.068
2.567SerPhe: 2.567 ± 0.061
4.928SerGly: 4.928 ± 0.092
1.402SerHis: 1.402 ± 0.04
3.669SerIle: 3.669 ± 0.076
2.513SerLys: 2.513 ± 0.052
6.873SerLeu: 6.873 ± 0.104
1.845SerMet: 1.845 ± 0.051
2.237SerAsn: 2.237 ± 0.048
2.491SerPro: 2.491 ± 0.055
2.76SerGln: 2.76 ± 0.058
3.2SerArg: 3.2 ± 0.068
3.804SerSer: 3.804 ± 0.077
2.962SerThr: 2.962 ± 0.067
4.257SerVal: 4.257 ± 0.072
0.896SerTrp: 0.896 ± 0.034
1.833SerTyr: 1.833 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
5.034ThrAla: 5.034 ± 0.079
0.345ThrCys: 0.345 ± 0.02
3.24ThrAsp: 3.24 ± 0.068
3.403ThrGlu: 3.403 ± 0.068
2.012ThrPhe: 2.012 ± 0.053
4.29ThrGly: 4.29 ± 0.08
1.188ThrHis: 1.188 ± 0.038
3.395ThrIle: 3.395 ± 0.07
1.816ThrLys: 1.816 ± 0.053
6.265ThrLeu: 6.265 ± 0.081
1.229ThrMet: 1.229 ± 0.038
1.88ThrAsn: 1.88 ± 0.045
2.635ThrPro: 2.635 ± 0.061
2.32ThrGln: 2.32 ± 0.05
2.373ThrArg: 2.373 ± 0.057
3.061ThrSer: 3.061 ± 0.058
2.841ThrThr: 2.841 ± 0.066
3.687ThrVal: 3.687 ± 0.066
0.452ThrTrp: 0.452 ± 0.025
1.172ThrTyr: 1.172 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
6.056ValAla: 6.056 ± 0.08
0.66ValCys: 0.66 ± 0.03
4.066ValAsp: 4.066 ± 0.083
4.229ValGlu: 4.229 ± 0.072
2.865ValPhe: 2.865 ± 0.064
4.322ValGly: 4.322 ± 0.076
1.258ValHis: 1.258 ± 0.037
5.07ValIle: 5.07 ± 0.086
2.854ValLys: 2.854 ± 0.059
6.797ValLeu: 6.797 ± 0.11
2.084ValMet: 2.084 ± 0.046
2.746ValAsn: 2.746 ± 0.065
2.475ValPro: 2.475 ± 0.059
2.209ValGln: 2.209 ± 0.048
3.074ValArg: 3.074 ± 0.057
4.417ValSer: 4.417 ± 0.077
3.818ValThr: 3.818 ± 0.073
4.88ValVal: 4.88 ± 0.098
0.821ValTrp: 0.821 ± 0.032
1.681ValTyr: 1.681 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.835TrpAla: 0.835 ± 0.034
0.147TrpCys: 0.147 ± 0.012
0.585TrpAsp: 0.585 ± 0.024
0.597TrpGlu: 0.597 ± 0.023
0.64TrpPhe: 0.64 ± 0.031
0.713TrpGly: 0.713 ± 0.033
0.421TrpHis: 0.421 ± 0.024
0.753TrpIle: 0.753 ± 0.035
0.466TrpLys: 0.466 ± 0.024
2.006TrpLeu: 2.006 ± 0.058
0.353TrpMet: 0.353 ± 0.02
0.478TrpAsn: 0.478 ± 0.024
0.565TrpPro: 0.565 ± 0.025
1.151TrpGln: 1.151 ± 0.041
0.781TrpArg: 0.781 ± 0.036
0.713TrpSer: 0.713 ± 0.029
0.547TrpThr: 0.547 ± 0.027
0.766TrpVal: 0.766 ± 0.027
0.194TrpTrp: 0.194 ± 0.015
0.332TrpTyr: 0.332 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.321TyrAla: 2.321 ± 0.057
0.295TyrCys: 0.295 ± 0.02
1.55TyrAsp: 1.55 ± 0.046
1.514TyrGlu: 1.514 ± 0.047
1.26TyrPhe: 1.26 ± 0.037
2.033TyrGly: 2.033 ± 0.052
0.711TyrHis: 0.711 ± 0.029
1.415TyrIle: 1.415 ± 0.043
1.029TyrLys: 1.029 ± 0.032
3.244TyrLeu: 3.244 ± 0.066
0.525TyrMet: 0.525 ± 0.026
0.92TyrAsn: 0.92 ± 0.034
1.33TyrPro: 1.33 ± 0.039
1.956TyrGln: 1.956 ± 0.055
1.812TyrArg: 1.812 ± 0.046
1.75TyrSer: 1.75 ± 0.045
1.243TyrThr: 1.243 ± 0.042
1.656TyrVal: 1.656 ± 0.048
0.422TyrTrp: 0.422 ± 0.023
0.83TyrTyr: 0.83 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2793 proteins (857838 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski