Blackberry virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S1YEA3|A0A2S1YEA3_9VIRU Replicase OS=Blackberry virus A OX=2185086 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.42SLGASSQRR9 pKa = 11.84VAYY12 pKa = 10.22ANLYY16 pKa = 10.56SNLGDD21 pKa = 4.58KK22 pKa = 10.49IEE24 pKa = 3.87QSKK27 pKa = 10.29VIKK30 pKa = 8.87ATRR33 pKa = 11.84VNEE36 pKa = 4.07HH37 pKa = 6.61EE38 pKa = 4.37EE39 pKa = 4.31SVSGKK44 pKa = 9.72FDD46 pKa = 3.57YY47 pKa = 10.69FISDD51 pKa = 3.07EE52 pKa = 4.54AYY54 pKa = 10.26DD55 pKa = 3.9YY56 pKa = 11.3LSSKK60 pKa = 10.16GVPLSAHH67 pKa = 7.38CYY69 pKa = 9.19RR70 pKa = 11.84PHH72 pKa = 6.6PHH74 pKa = 7.55PISKK78 pKa = 9.66MIEE81 pKa = 3.5NHH83 pKa = 5.62ILFNVLPNYY92 pKa = 9.67LRR94 pKa = 11.84PRR96 pKa = 11.84NLFISIKK103 pKa = 7.56EE104 pKa = 3.92DD105 pKa = 3.11KK106 pKa = 9.33MVGVISVARR115 pKa = 11.84NRR117 pKa = 11.84NKK119 pKa = 9.85VDD121 pKa = 3.16AVSNSSVYY129 pKa = 10.64NRR131 pKa = 11.84LIHH134 pKa = 7.0AKK136 pKa = 10.46DD137 pKa = 2.99SLRR140 pKa = 11.84YY141 pKa = 8.89KK142 pKa = 11.0DD143 pKa = 3.76PVRR146 pKa = 11.84DD147 pKa = 4.12LDD149 pKa = 4.42LSLNKK154 pKa = 9.79NLKK157 pKa = 10.18EE158 pKa = 4.35KK159 pKa = 10.29MNQADD164 pKa = 3.78LVVIHH169 pKa = 7.26DD170 pKa = 4.24EE171 pKa = 3.92VHH173 pKa = 5.23YY174 pKa = 9.55WALVDD179 pKa = 4.05FQKK182 pKa = 11.03FLGLSNKK189 pKa = 9.0PVLYY193 pKa = 10.58SIIYY197 pKa = 9.29PPEE200 pKa = 4.7LSAGIDD206 pKa = 3.52YY207 pKa = 10.93SLNPMLYY214 pKa = 8.48EE215 pKa = 4.08FKK217 pKa = 11.0VNKK220 pKa = 9.84SDD222 pKa = 3.62SFTWAPDD229 pKa = 3.17GSYY232 pKa = 10.79NGAYY236 pKa = 6.82TQPINPRR243 pKa = 11.84LLTTMKK249 pKa = 10.95LEE251 pKa = 4.54DD252 pKa = 4.21PNGNSWTLTKK262 pKa = 10.77LEE264 pKa = 4.59TIGAHH269 pKa = 5.63HH270 pKa = 6.99VFLATPGSLVTEE282 pKa = 4.51EE283 pKa = 4.23SYY285 pKa = 11.25IMRR288 pKa = 11.84IHH290 pKa = 6.42PHH292 pKa = 6.56PPGKK296 pKa = 9.92HH297 pKa = 5.79SGPTQHH303 pKa = 6.01KK304 pKa = 8.29TKK306 pKa = 10.74VEE308 pKa = 4.24GEE310 pKa = 4.22FGQPDD315 pKa = 3.86APSAISKK322 pKa = 9.62IRR324 pKa = 11.84QLTRR328 pKa = 11.84GDD330 pKa = 3.45EE331 pKa = 4.16TAEE334 pKa = 3.84EE335 pKa = 4.18IMFVGRR341 pKa = 11.84LARR344 pKa = 11.84QISNTKK350 pKa = 10.34CFGEE354 pKa = 4.76DD355 pKa = 2.97GLFRR359 pKa = 11.84FGKK362 pKa = 10.44EE363 pKa = 3.42MLLTLEE369 pKa = 4.64HH370 pKa = 7.01VMGAKK375 pKa = 8.75FTYY378 pKa = 10.78LLDD381 pKa = 3.99KK382 pKa = 11.58NEE384 pKa = 3.73FDD386 pKa = 3.56VWNLEE391 pKa = 4.08STLKK395 pKa = 10.72LLCKK399 pKa = 10.07PKK401 pKa = 9.35ITIEE405 pKa = 3.94RR406 pKa = 11.84HH407 pKa = 3.89FRR409 pKa = 11.84AYY411 pKa = 7.99TAPDD415 pKa = 3.42HH416 pKa = 6.36EE417 pKa = 5.4LPLEE421 pKa = 4.41DD422 pKa = 4.92LHH424 pKa = 6.99NALNGFLDD432 pKa = 4.21GKK434 pKa = 10.57RR435 pKa = 11.84RR436 pKa = 11.84LINSNEE442 pKa = 3.87WVVLPDD448 pKa = 3.7RR449 pKa = 11.84EE450 pKa = 4.17PRR452 pKa = 11.84PYY454 pKa = 10.47SALGKK459 pKa = 10.08KK460 pKa = 9.66EE461 pKa = 3.83RR462 pKa = 11.84DD463 pKa = 3.85DD464 pKa = 4.14VLHH467 pKa = 6.61HH468 pKa = 6.05ALKK471 pKa = 9.59RR472 pKa = 11.84TEE474 pKa = 3.82EE475 pKa = 4.14LKK477 pKa = 10.75RR478 pKa = 11.84VIGANPVVVKK488 pKa = 10.62NLNQRR493 pKa = 11.84YY494 pKa = 8.82YY495 pKa = 10.85CEE497 pKa = 4.0QCPTPIFKK505 pKa = 10.78KK506 pKa = 10.21NLQKK510 pKa = 10.54VRR512 pKa = 11.84TNIYY516 pKa = 9.81MEE518 pKa = 4.49VPCIGTKK525 pKa = 10.21RR526 pKa = 11.84PIEE529 pKa = 3.94LLKK532 pKa = 10.88EE533 pKa = 3.85GLINEE538 pKa = 4.35EE539 pKa = 4.01EE540 pKa = 4.56YY541 pKa = 10.61EE542 pKa = 4.47RR543 pKa = 11.84RR544 pKa = 11.84MQTPTEE550 pKa = 3.9RR551 pKa = 11.84VEE553 pKa = 4.59APEE556 pKa = 4.03SDD558 pKa = 3.52IEE560 pKa = 4.37SEE562 pKa = 4.45STVSCGEE569 pKa = 4.01EE570 pKa = 3.89DD571 pKa = 4.2NDD573 pKa = 3.67WLEE576 pKa = 4.03NVTKK580 pKa = 10.64IKK582 pKa = 10.95GLVKK586 pKa = 10.15NVCLLGPISEE596 pKa = 4.12AMKK599 pKa = 10.07MDD601 pKa = 3.49VATLLSACNSMDD613 pKa = 3.66PNFARR618 pKa = 11.84FISNSGLSMNGFVMMIRR635 pKa = 11.84MLDD638 pKa = 3.36LTISIKK644 pKa = 10.38KK645 pKa = 9.76GPYY648 pKa = 8.02RR649 pKa = 11.84YY650 pKa = 9.69SRR652 pKa = 11.84KK653 pKa = 9.92GLRR656 pKa = 11.84APFGITVNKK665 pKa = 10.2DD666 pKa = 2.85HH667 pKa = 7.69AEE669 pKa = 3.94KK670 pKa = 10.86CDD672 pKa = 3.83FVDD675 pKa = 4.86FGDD678 pKa = 3.78RR679 pKa = 11.84TTDD682 pKa = 3.65LLLMGPNVGICSMLITGKK700 pKa = 9.92SANLIRR706 pKa = 11.84DD707 pKa = 4.2SFQKK711 pKa = 10.67NYY713 pKa = 9.75TGIVLCDD720 pKa = 4.28LKK722 pKa = 11.36NGLPTFDD729 pKa = 3.46QNYY732 pKa = 8.92SEE734 pKa = 4.87VIRR737 pKa = 11.84VITTLGFAGSGKK749 pKa = 10.12SSYY752 pKa = 8.85LTQVLHH758 pKa = 7.31LSRR761 pKa = 11.84EE762 pKa = 4.12HH763 pKa = 6.49CAVVSPRR770 pKa = 11.84TNLAAEE776 pKa = 4.01WKK778 pKa = 9.02KK779 pKa = 8.96WLKK782 pKa = 8.9GTSHH786 pKa = 5.82QVHH789 pKa = 5.84TFEE792 pKa = 5.12TYY794 pKa = 10.87LKK796 pKa = 10.57VGATGPRR803 pKa = 11.84LLVFDD808 pKa = 4.96EE809 pKa = 5.41APLFPPGYY817 pKa = 8.78IDD819 pKa = 4.36LAIMMKK825 pKa = 10.36RR826 pKa = 11.84PTDD829 pKa = 3.67VVILGDD835 pKa = 4.0PLQCSYY841 pKa = 11.15HH842 pKa = 5.65SEE844 pKa = 3.91KK845 pKa = 10.81DD846 pKa = 3.46RR847 pKa = 11.84LKK849 pKa = 10.83LSPIEE854 pKa = 3.67PSIFKK859 pKa = 10.59RR860 pKa = 11.84LGGASIQCQCGTKK873 pKa = 9.91ILVSAAKK880 pKa = 10.27ANEE883 pKa = 3.84PTAVGEE889 pKa = 3.92PDD891 pKa = 3.83SIKK894 pKa = 10.59GRR896 pKa = 11.84QIWFYY901 pKa = 10.64SRR903 pKa = 11.84NGEE906 pKa = 4.19GYY908 pKa = 9.28KK909 pKa = 9.17YY910 pKa = 9.26TGAEE914 pKa = 4.2HH915 pKa = 7.18ASKK918 pKa = 10.41GWPEE922 pKa = 4.11EE923 pKa = 3.77LDD925 pKa = 4.89DD926 pKa = 4.08ILVSHH931 pKa = 6.96GYY933 pKa = 9.12DD934 pKa = 2.55PKK936 pKa = 10.78MFNHH940 pKa = 6.91CLFQMFEE947 pKa = 3.72AGARR951 pKa = 11.84IPYY954 pKa = 9.5HH955 pKa = 7.05ADD957 pKa = 3.33DD958 pKa = 4.89EE959 pKa = 4.77PVLTLRR965 pKa = 11.84GNPILTISSGKK976 pKa = 7.5STHH979 pKa = 6.55GFSCKK984 pKa = 9.68QGSIEE989 pKa = 4.31FDD991 pKa = 3.15SSSASFLMPNDD1002 pKa = 3.34FQKK1005 pKa = 8.81THH1007 pKa = 5.09KK1008 pKa = 9.79HH1009 pKa = 5.0CVGARR1014 pKa = 11.84GPRR1017 pKa = 11.84MSWTFRR1023 pKa = 11.84STEE1026 pKa = 4.63PITITPKK1033 pKa = 10.17KK1034 pKa = 10.55DD1035 pKa = 2.92RR1036 pKa = 11.84VPYY1039 pKa = 10.69LLFSHH1044 pKa = 7.45RR1045 pKa = 11.84LPEE1048 pKa = 4.09KK1049 pKa = 10.05QKK1051 pKa = 10.47LFEE1054 pKa = 4.39GLRR1057 pKa = 11.84CAGPGEE1063 pKa = 4.37LKK1065 pKa = 10.59LAEE1068 pKa = 4.48LPQEE1072 pKa = 4.22RR1073 pKa = 11.84GGDD1076 pKa = 3.2KK1077 pKa = 10.4TLTFKK1082 pKa = 10.76RR1083 pKa = 11.84RR1084 pKa = 11.84EE1085 pKa = 4.01HH1086 pKa = 7.12VDD1088 pKa = 3.06EE1089 pKa = 4.4NKK1091 pKa = 10.21IMTVGQSQGLSLDD1104 pKa = 3.46VVQIVFDD1111 pKa = 4.25AGALKK1116 pKa = 10.13TSGQTMITALTRR1128 pKa = 11.84ARR1130 pKa = 11.84EE1131 pKa = 4.7GINMVFPLGRR1141 pKa = 11.84DD1142 pKa = 3.55YY1143 pKa = 10.59TAKK1146 pKa = 10.35NARR1149 pKa = 11.84DD1150 pKa = 3.53PFVRR1154 pKa = 11.84AVAEE1158 pKa = 3.69KK1159 pKa = 10.87RR1160 pKa = 11.84MIGKK1164 pKa = 9.04QEE1166 pKa = 3.93LMNLIKK1172 pKa = 10.41DD1173 pKa = 3.63SCSFDD1178 pKa = 3.42VEE1180 pKa = 4.33YY1181 pKa = 11.1LEE1183 pKa = 4.44EE1184 pKa = 3.99EE1185 pKa = 4.73LYY1187 pKa = 10.51IGLTMDD1193 pKa = 3.45EE1194 pKa = 4.83VEE1196 pKa = 5.34DD1197 pKa = 4.68KK1198 pKa = 11.37LDD1200 pKa = 3.94ADD1202 pKa = 4.65PYY1204 pKa = 9.9LKK1206 pKa = 11.05AMLLMLEE1213 pKa = 4.23HH1214 pKa = 7.12EE1215 pKa = 4.62EE1216 pKa = 3.9MDD1218 pKa = 3.67EE1219 pKa = 4.31EE1220 pKa = 4.17EE1221 pKa = 4.5MEE1223 pKa = 5.15EE1224 pKa = 4.26EE1225 pKa = 4.29FCPEE1229 pKa = 3.86GSRR1232 pKa = 11.84THH1234 pKa = 6.62LPLSEE1239 pKa = 4.06FTNEE1243 pKa = 3.73QFASEE1248 pKa = 4.36LKK1250 pKa = 10.58AKK1252 pKa = 9.17EE1253 pKa = 3.88EE1254 pKa = 4.15RR1255 pKa = 11.84EE1256 pKa = 4.01QYY1258 pKa = 10.31HH1259 pKa = 5.36YY1260 pKa = 11.04QLGYY1264 pKa = 10.61SNQIRR1269 pKa = 11.84DD1270 pKa = 4.22DD1271 pKa = 3.62ISGEE1275 pKa = 4.06RR1276 pKa = 11.84YY1277 pKa = 9.81AGPEE1281 pKa = 3.72KK1282 pKa = 10.59SRR1284 pKa = 11.84SSIYY1288 pKa = 9.73MKK1290 pKa = 9.74HH1291 pKa = 5.9TDD1293 pKa = 3.15SKK1295 pKa = 11.2NVKK1298 pKa = 7.77EE1299 pKa = 4.32TQILRR1304 pKa = 11.84LRR1306 pKa = 11.84EE1307 pKa = 4.06EE1308 pKa = 4.13QEE1310 pKa = 4.13KK1311 pKa = 10.62LLQGLPHH1318 pKa = 6.73WKK1320 pKa = 8.49VHH1322 pKa = 4.5VWNLCRR1328 pKa = 11.84KK1329 pKa = 9.97LEE1331 pKa = 4.18FPEE1334 pKa = 3.98VAPAMEE1340 pKa = 4.44LTDD1343 pKa = 3.98KK1344 pKa = 10.85EE1345 pKa = 4.66LEE1347 pKa = 4.11FTEE1350 pKa = 5.34KK1351 pKa = 10.3RR1352 pKa = 11.84VQKK1355 pKa = 10.75SKK1357 pKa = 11.31ALLEE1361 pKa = 4.05AHH1363 pKa = 7.66GYY1365 pKa = 10.25RR1366 pKa = 11.84SDD1368 pKa = 4.34PDD1370 pKa = 3.3WPSNILRR1377 pKa = 11.84VFIKK1381 pKa = 10.7NQTCTKK1387 pKa = 8.81MEE1389 pKa = 4.33KK1390 pKa = 9.96RR1391 pKa = 11.84GCDD1394 pKa = 3.06AKK1396 pKa = 10.89AGQTIACFSHH1406 pKa = 6.94AVLCKK1411 pKa = 10.46FGPALRR1417 pKa = 11.84RR1418 pKa = 11.84TEE1420 pKa = 3.97AQFRR1424 pKa = 11.84ALLPEE1429 pKa = 3.89STLIFSQKK1437 pKa = 10.73NYY1439 pKa = 10.58DD1440 pKa = 6.4DD1441 pKa = 4.79LDD1443 pKa = 4.0EE1444 pKa = 4.24WCKK1447 pKa = 10.42TYY1449 pKa = 10.75FNDD1452 pKa = 5.59LSGTDD1457 pKa = 3.22SDD1459 pKa = 5.03YY1460 pKa = 11.59EE1461 pKa = 4.18AFDD1464 pKa = 3.94RR1465 pKa = 11.84SQDD1468 pKa = 3.32ATILGMEE1475 pKa = 4.25VEE1477 pKa = 4.24MLKK1480 pKa = 10.73YY1481 pKa = 10.35FGWEE1485 pKa = 3.55QEE1487 pKa = 4.59LISEE1491 pKa = 4.23YY1492 pKa = 10.89TEE1494 pKa = 4.09LKK1496 pKa = 11.05LMMGCTLGEE1505 pKa = 3.96LSVMRR1510 pKa = 11.84FSGEE1514 pKa = 3.44FGTFFFNTVCNMAYY1528 pKa = 9.47TYY1530 pKa = 9.95MRR1532 pKa = 11.84YY1533 pKa = 9.99DD1534 pKa = 2.9IRR1536 pKa = 11.84KK1537 pKa = 8.07DD1538 pKa = 3.32HH1539 pKa = 7.3PIAFAGDD1546 pKa = 3.66DD1547 pKa = 3.49MVAPGRR1553 pKa = 11.84LIQTCAHH1560 pKa = 6.46NDD1562 pKa = 3.52IIDD1565 pKa = 4.0KK1566 pKa = 9.16LTLKK1570 pKa = 11.02AKK1572 pKa = 10.19VNYY1575 pKa = 10.17SNEE1578 pKa = 3.82PLFCGWRR1585 pKa = 11.84VSPLGIAKK1593 pKa = 10.09DD1594 pKa = 3.79PNLLLDD1600 pKa = 3.58RR1601 pKa = 11.84WEE1603 pKa = 4.13MAVEE1607 pKa = 3.78QGRR1610 pKa = 11.84LHH1612 pKa = 6.98EE1613 pKa = 4.6CMCNYY1618 pKa = 10.44ALEE1621 pKa = 4.26ASYY1624 pKa = 10.54GYY1626 pKa = 10.36RR1627 pKa = 11.84LSDD1630 pKa = 3.94SLFEE1634 pKa = 4.71LNIDD1638 pKa = 3.01IDD1640 pKa = 4.18AYY1642 pKa = 10.86QEE1644 pKa = 4.22LIRR1647 pKa = 11.84KK1648 pKa = 6.42VVKK1651 pKa = 10.44VKK1653 pKa = 10.7RR1654 pKa = 11.84KK1655 pKa = 9.94LPEE1658 pKa = 4.06QISKK1662 pKa = 10.39LFSGHH1667 pKa = 7.29DD1668 pKa = 4.5DD1669 pKa = 3.15IDD1671 pKa = 4.16SAGEE1675 pKa = 3.75EE1676 pKa = 4.17

Molecular weight:
190.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S1YE91|A0A2S1YE91_9VIRU Uncharacterized protein OS=Blackberry virus A OX=2185086 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.77SYY4 pKa = 11.33LLGEE8 pKa = 4.68SKK10 pKa = 10.61SAAKK14 pKa = 9.99RR15 pKa = 11.84RR16 pKa = 11.84ARR18 pKa = 11.84RR19 pKa = 11.84WGVCYY24 pKa = 10.33CHH26 pKa = 6.84GRR28 pKa = 11.84LEE30 pKa = 4.31RR31 pKa = 11.84ANKK34 pKa = 7.2QTNAQVEE41 pKa = 4.5VEE43 pKa = 4.09RR44 pKa = 11.84ALRR47 pKa = 11.84GPATRR52 pKa = 11.84YY53 pKa = 7.87QSKK56 pKa = 10.49VGQCYY61 pKa = 6.88VTNAIQHH68 pKa = 6.39CLDD71 pKa = 4.36DD72 pKa = 3.4MHH74 pKa = 7.58KK75 pKa = 10.71RR76 pKa = 11.84GDD78 pKa = 3.41HH79 pKa = 5.52TFDD82 pKa = 3.31NKK84 pKa = 10.46FKK86 pKa = 10.91INGPAYY92 pKa = 9.44GSKK95 pKa = 10.37SPEE98 pKa = 3.93EE99 pKa = 4.2TPWAYY104 pKa = 11.22NWTNTT109 pKa = 4.17

Molecular weight:
12.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2404

109

1676

480.8

54.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.616 ± 0.951

2.121 ± 0.328

5.782 ± 0.716

7.903 ± 0.366

4.285 ± 0.379

6.572 ± 0.569

2.621 ± 0.397

4.992 ± 0.572

7.196 ± 0.37

10.025 ± 0.873

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.238

4.16 ± 0.603

4.451 ± 0.731

2.787 ± 0.346

5.907 ± 0.649

6.864 ± 0.588

5.2 ± 0.287

6.364 ± 1.472

0.915 ± 0.336

3.411 ± 0.56

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski