Gordonia phage Walrus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481S299|A0A481S299_9CAUD Immunity repressor OS=Gordonia phage Walrus OX=2517927 GN=41 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.29LPEE11 pKa = 4.28IPNVTFTASYY21 pKa = 10.7GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.99NPWYY45 pKa = 10.23EE46 pKa = 3.57PDD48 pKa = 3.52YY49 pKa = 11.49NYY51 pKa = 11.41GLDD54 pKa = 3.96PNGHH58 pKa = 5.52TEE60 pKa = 4.34ITDD63 pKa = 2.83PWKK66 pKa = 10.41RR67 pKa = 11.84HH68 pKa = 3.46TRR70 pKa = 11.84YY71 pKa = 10.11PEE73 pKa = 3.85VCSMGFGGPASIGLPADD90 pKa = 3.74PPPPVEE96 pKa = 4.79PEE98 pKa = 3.75PTPDD102 pKa = 3.64IEE104 pKa = 4.54EE105 pKa = 4.49PTDD108 pKa = 3.42GG109 pKa = 4.69

Molecular weight: 11.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481S2B3|A0A481S2B3_9CAUD Uncharacterized protein OS=Gordonia phage Walrus OX=2517927 GN=22 PE=4 SV=1
MM1 pKa = 7.73PFVLLAVANFALFLLALAAILTGGTLLLAAAWALLIVEE39 pKa = 4.63ALSVVGWARR48 pKa = 11.84LAAHH52 pKa = 8.04RR53 pKa = 11.84ARR55 pKa = 11.84KK56 pKa = 9.38DD57 pKa = 3.34LPQQ60 pKa = 5.03

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16728

54

1840

199.1

21.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.352 ± 0.477

0.855 ± 0.136

7.162 ± 0.29

5.434 ± 0.301

2.792 ± 0.157

8.782 ± 0.319

2.23 ± 0.178

4.573 ± 0.151

3.246 ± 0.249

7.455 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.185

2.983 ± 0.144

5.99 ± 0.261

3.473 ± 0.174

7.425 ± 0.362

5.099 ± 0.217

6.946 ± 0.23

7.64 ± 0.265

2.098 ± 0.113

2.331 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski