Gordonia phage Walrus
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A481S299|A0A481S299_9CAUD Immunity repressor OS=Gordonia phage Walrus OX=2517927 GN=41 PE=4 SV=1
MM1 pKa = 7.46 ANDD4 pKa = 4.61 TIFEE8 pKa = 4.29 LPEE11 pKa = 4.28 IPNVTFTASYY21 pKa = 10.7 GSGGEE26 pKa = 4.12 TGLPSNWIRR35 pKa = 11.84 IVGTVEE41 pKa = 3.99 NPWYY45 pKa = 10.23 EE46 pKa = 3.57 PDD48 pKa = 3.52 YY49 pKa = 11.49 NYY51 pKa = 11.41 GLDD54 pKa = 3.96 PNGHH58 pKa = 5.52 TEE60 pKa = 4.34 ITDD63 pKa = 2.83 PWKK66 pKa = 10.41 RR67 pKa = 11.84 HH68 pKa = 3.46 TRR70 pKa = 11.84 YY71 pKa = 10.11 PEE73 pKa = 3.85 VCSMGFGGPASIGLPADD90 pKa = 3.74 PPPPVEE96 pKa = 4.79 PEE98 pKa = 3.75 PTPDD102 pKa = 3.64 IEE104 pKa = 4.54 EE105 pKa = 4.49 PTDD108 pKa = 3.42 GG109 pKa = 4.69
Molecular weight: 11.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.986
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.745
EMBOSS 3.757
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.834
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A481S2B3|A0A481S2B3_9CAUD Uncharacterized protein OS=Gordonia phage Walrus OX=2517927 GN=22 PE=4 SV=1
MM1 pKa = 7.73 PFVLLAVANFALFLLALAAILTGGTLLLAAAWALLIVEE39 pKa = 4.63 ALSVVGWARR48 pKa = 11.84 LAAHH52 pKa = 8.04 RR53 pKa = 11.84 ARR55 pKa = 11.84 KK56 pKa = 9.38 DD57 pKa = 3.34 LPQQ60 pKa = 5.03
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.414
IPC_protein 10.365
Toseland 10.95
ProMoST 10.906
Dawson 10.965
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.023
Grimsley 10.994
Solomon 11.272
Lehninger 11.228
Nozaki 10.921
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.921
Patrickios 11.023
IPC_peptide 11.272
IPC2_peptide 9.692
IPC2.peptide.svr19 8.938
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
16728
54
1840
199.1
21.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.352 ± 0.477
0.855 ± 0.136
7.162 ± 0.29
5.434 ± 0.301
2.792 ± 0.157
8.782 ± 0.319
2.23 ± 0.178
4.573 ± 0.151
3.246 ± 0.249
7.455 ± 0.263
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.134 ± 0.185
2.983 ± 0.144
5.99 ± 0.261
3.473 ± 0.174
7.425 ± 0.362
5.099 ± 0.217
6.946 ± 0.23
7.64 ± 0.265
2.098 ± 0.113
2.331 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here