Amino acid dipepetide frequency for Gordonia phage Walrus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.44AlaAla: 16.44 ± 1.386
0.538AlaCys: 0.538 ± 0.19
8.31AlaAsp: 8.31 ± 0.79
6.397AlaGlu: 6.397 ± 0.78
3.527AlaPhe: 3.527 ± 0.588
9.326AlaGly: 9.326 ± 0.924
2.152AlaHis: 2.152 ± 0.375
5.261AlaIle: 5.261 ± 0.556
3.886AlaLys: 3.886 ± 0.685
9.446AlaLeu: 9.446 ± 0.885
2.332AlaMet: 2.332 ± 0.536
3.109AlaAsn: 3.109 ± 0.5
4.962AlaPro: 4.962 ± 0.524
4.842AlaGln: 4.842 ± 0.607
7.951AlaArg: 7.951 ± 0.818
5.859AlaSer: 5.859 ± 0.847
7.652AlaThr: 7.652 ± 0.695
7.772AlaVal: 7.772 ± 0.785
2.152AlaTrp: 2.152 ± 0.293
1.614AlaTyr: 1.614 ± 0.321
0.0AlaXaa: 0.0 ± 0.0
Cys
0.538CysAla: 0.538 ± 0.169
0.179CysCys: 0.179 ± 0.134
1.076CysAsp: 1.076 ± 0.322
0.299CysGlu: 0.299 ± 0.144
0.06CysPhe: 0.06 ± 0.062
0.777CysGly: 0.777 ± 0.216
0.179CysHis: 0.179 ± 0.116
0.359CysIle: 0.359 ± 0.181
0.299CysLys: 0.299 ± 0.142
0.179CysLeu: 0.179 ± 0.108
0.299CysMet: 0.299 ± 0.154
0.418CysAsn: 0.418 ± 0.143
0.717CysPro: 0.717 ± 0.216
0.478CysGln: 0.478 ± 0.156
0.658CysArg: 0.658 ± 0.171
0.359CysSer: 0.359 ± 0.127
0.598CysThr: 0.598 ± 0.206
0.717CysVal: 0.717 ± 0.245
0.299CysTrp: 0.299 ± 0.168
0.06CysTyr: 0.06 ± 0.058
0.0CysXaa: 0.0 ± 0.0
Asp
7.174AspAla: 7.174 ± 0.814
0.299AspCys: 0.299 ± 0.169
5.978AspAsp: 5.978 ± 0.86
5.261AspGlu: 5.261 ± 0.704
1.973AspPhe: 1.973 ± 0.334
7.114AspGly: 7.114 ± 0.92
2.152AspHis: 2.152 ± 0.389
2.929AspIle: 2.929 ± 0.439
1.196AspLys: 1.196 ± 0.345
7.114AspLeu: 7.114 ± 0.828
1.136AspMet: 1.136 ± 0.247
2.212AspAsn: 2.212 ± 0.379
4.185AspPro: 4.185 ± 0.578
2.929AspGln: 2.929 ± 0.398
4.603AspArg: 4.603 ± 0.735
3.587AspSer: 3.587 ± 0.528
3.766AspThr: 3.766 ± 0.461
4.962AspVal: 4.962 ± 0.545
0.957AspTrp: 0.957 ± 0.232
2.033AspTyr: 2.033 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
5.799GluAla: 5.799 ± 0.726
0.239GluCys: 0.239 ± 0.114
2.212GluAsp: 2.212 ± 0.389
2.511GluGlu: 2.511 ± 0.535
2.212GluPhe: 2.212 ± 0.31
3.527GluGly: 3.527 ± 0.437
1.196GluHis: 1.196 ± 0.263
2.929GluIle: 2.929 ± 0.378
1.794GluLys: 1.794 ± 0.311
5.56GluLeu: 5.56 ± 0.822
1.375GluMet: 1.375 ± 0.298
1.554GluAsn: 1.554 ± 0.357
4.125GluPro: 4.125 ± 0.783
2.212GluGln: 2.212 ± 0.415
4.603GluArg: 4.603 ± 0.567
2.511GluSer: 2.511 ± 0.302
2.989GluThr: 2.989 ± 0.364
4.484GluVal: 4.484 ± 0.559
1.196GluTrp: 1.196 ± 0.273
1.315GluTyr: 1.315 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
2.87PheAla: 2.87 ± 0.392
0.299PheCys: 0.299 ± 0.157
2.929PheAsp: 2.929 ± 0.52
1.495PheGlu: 1.495 ± 0.249
0.897PhePhe: 0.897 ± 0.233
2.81PheGly: 2.81 ± 0.412
0.598PheHis: 0.598 ± 0.186
0.897PheIle: 0.897 ± 0.292
0.957PheLys: 0.957 ± 0.327
2.092PheLeu: 2.092 ± 0.365
0.777PheMet: 0.777 ± 0.207
0.957PheAsn: 0.957 ± 0.275
1.315PhePro: 1.315 ± 0.241
0.717PheGln: 0.717 ± 0.169
1.794PheArg: 1.794 ± 0.304
1.495PheSer: 1.495 ± 0.334
2.033PheThr: 2.033 ± 0.344
2.152PheVal: 2.152 ± 0.322
0.12PheTrp: 0.12 ± 0.08
0.717PheTyr: 0.717 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
8.19GlyAla: 8.19 ± 0.982
0.538GlyCys: 0.538 ± 0.166
5.679GlyAsp: 5.679 ± 0.523
4.783GlyGlu: 4.783 ± 0.654
3.348GlyPhe: 3.348 ± 0.451
8.011GlyGly: 8.011 ± 0.803
1.315GlyHis: 1.315 ± 0.279
3.647GlyIle: 3.647 ± 0.593
3.587GlyLys: 3.587 ± 0.637
7.533GlyLeu: 7.533 ± 1.127
1.614GlyMet: 1.614 ± 0.262
2.63GlyAsn: 2.63 ± 0.508
4.304GlyPro: 4.304 ± 0.631
2.929GlyGln: 2.929 ± 0.399
7.413GlyArg: 7.413 ± 0.669
4.902GlySer: 4.902 ± 0.584
5.321GlyThr: 5.321 ± 0.583
7.054GlyVal: 7.054 ± 0.625
2.272GlyTrp: 2.272 ± 0.346
2.75GlyTyr: 2.75 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
2.272HisAla: 2.272 ± 0.447
0.359HisCys: 0.359 ± 0.156
2.212HisAsp: 2.212 ± 0.391
0.717HisGlu: 0.717 ± 0.236
0.777HisPhe: 0.777 ± 0.216
1.973HisGly: 1.973 ± 0.37
0.418HisHis: 0.418 ± 0.172
1.076HisIle: 1.076 ± 0.305
0.478HisLys: 0.478 ± 0.171
1.674HisLeu: 1.674 ± 0.334
0.239HisMet: 0.239 ± 0.111
0.239HisAsn: 0.239 ± 0.131
1.913HisPro: 1.913 ± 0.4
0.658HisGln: 0.658 ± 0.166
1.913HisArg: 1.913 ± 0.377
1.136HisSer: 1.136 ± 0.231
1.853HisThr: 1.853 ± 0.419
1.435HisVal: 1.435 ± 0.278
0.359HisTrp: 0.359 ± 0.153
0.598HisTyr: 0.598 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
6.397IleAla: 6.397 ± 0.676
0.239IleCys: 0.239 ± 0.124
4.185IleAsp: 4.185 ± 0.553
2.212IleGlu: 2.212 ± 0.325
0.837IlePhe: 0.837 ± 0.227
4.663IleGly: 4.663 ± 0.793
0.658IleHis: 0.658 ± 0.221
1.614IleIle: 1.614 ± 0.379
1.913IleLys: 1.913 ± 0.459
2.69IleLeu: 2.69 ± 0.422
0.359IleMet: 0.359 ± 0.127
1.196IleAsn: 1.196 ± 0.222
2.87IlePro: 2.87 ± 0.381
1.016IleGln: 1.016 ± 0.223
4.065IleArg: 4.065 ± 0.547
1.674IleSer: 1.674 ± 0.335
3.766IleThr: 3.766 ± 0.503
4.065IleVal: 4.065 ± 0.436
0.359IleTrp: 0.359 ± 0.123
0.957IleTyr: 0.957 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
3.049LysAla: 3.049 ± 0.47
0.239LysCys: 0.239 ± 0.121
1.973LysAsp: 1.973 ± 0.432
1.495LysGlu: 1.495 ± 0.326
1.375LysPhe: 1.375 ± 0.303
2.391LysGly: 2.391 ± 0.387
0.717LysHis: 0.717 ± 0.233
1.734LysIle: 1.734 ± 0.362
2.033LysLys: 2.033 ± 0.363
2.75LysLeu: 2.75 ± 0.498
0.658LysMet: 0.658 ± 0.286
0.957LysAsn: 0.957 ± 0.244
2.63LysPro: 2.63 ± 0.337
0.717LysGln: 0.717 ± 0.257
1.913LysArg: 1.913 ± 0.329
1.554LysSer: 1.554 ± 0.346
2.152LysThr: 2.152 ± 0.4
2.511LysVal: 2.511 ± 0.363
0.777LysTrp: 0.777 ± 0.177
0.837LysTyr: 0.837 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
10.582LeuAla: 10.582 ± 1.166
0.598LeuCys: 0.598 ± 0.196
5.082LeuAsp: 5.082 ± 0.655
3.228LeuGlu: 3.228 ± 0.567
2.152LeuPhe: 2.152 ± 0.295
6.516LeuGly: 6.516 ± 0.686
1.375LeuHis: 1.375 ± 0.322
2.75LeuIle: 2.75 ± 0.379
1.913LeuLys: 1.913 ± 0.343
4.424LeuLeu: 4.424 ± 0.679
1.853LeuMet: 1.853 ± 0.406
2.391LeuAsn: 2.391 ± 0.453
4.424LeuPro: 4.424 ± 0.528
2.092LeuGln: 2.092 ± 0.261
4.962LeuArg: 4.962 ± 0.561
4.783LeuSer: 4.783 ± 0.601
6.038LeuThr: 6.038 ± 0.63
6.038LeuVal: 6.038 ± 0.572
1.913LeuTrp: 1.913 ± 0.378
1.435LeuTyr: 1.435 ± 0.263
0.0LeuXaa: 0.0 ± 0.0
Met
3.169MetAla: 3.169 ± 0.567
0.299MetCys: 0.299 ± 0.124
0.538MetAsp: 0.538 ± 0.199
0.717MetGlu: 0.717 ± 0.195
0.658MetPhe: 0.658 ± 0.154
1.853MetGly: 1.853 ± 0.327
0.418MetHis: 0.418 ± 0.148
1.136MetIle: 1.136 ± 0.294
0.717MetLys: 0.717 ± 0.194
1.375MetLeu: 1.375 ± 0.338
0.239MetMet: 0.239 ± 0.122
0.299MetAsn: 0.299 ± 0.12
1.554MetPro: 1.554 ± 0.314
0.538MetGln: 0.538 ± 0.241
1.674MetArg: 1.674 ± 0.517
1.495MetSer: 1.495 ± 0.334
2.929MetThr: 2.929 ± 0.4
0.777MetVal: 0.777 ± 0.254
0.658MetTrp: 0.658 ± 0.289
0.299MetTyr: 0.299 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
2.989AsnAla: 2.989 ± 0.434
0.299AsnCys: 0.299 ± 0.128
1.853AsnAsp: 1.853 ± 0.274
0.897AsnGlu: 0.897 ± 0.182
0.598AsnPhe: 0.598 ± 0.179
3.647AsnGly: 3.647 ± 0.554
0.837AsnHis: 0.837 ± 0.203
1.255AsnIle: 1.255 ± 0.304
0.658AsnLys: 0.658 ± 0.223
2.033AsnLeu: 2.033 ± 0.366
0.598AsnMet: 0.598 ± 0.193
0.837AsnAsn: 0.837 ± 0.234
3.228AsnPro: 3.228 ± 0.449
0.777AsnGln: 0.777 ± 0.178
2.033AsnArg: 2.033 ± 0.386
1.554AsnSer: 1.554 ± 0.259
2.451AsnThr: 2.451 ± 0.46
2.391AsnVal: 2.391 ± 0.379
0.359AsnTrp: 0.359 ± 0.12
0.538AsnTyr: 0.538 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
6.158ProAla: 6.158 ± 0.675
0.897ProCys: 0.897 ± 0.266
4.723ProAsp: 4.723 ± 0.692
4.245ProGlu: 4.245 ± 0.638
1.435ProPhe: 1.435 ± 0.301
4.783ProGly: 4.783 ± 0.626
1.255ProHis: 1.255 ± 0.275
3.228ProIle: 3.228 ± 0.416
2.391ProLys: 2.391 ± 0.38
3.348ProLeu: 3.348 ± 0.444
1.674ProMet: 1.674 ± 0.469
1.734ProAsn: 1.734 ± 0.296
4.006ProPro: 4.006 ± 0.834
2.092ProGln: 2.092 ± 0.416
3.408ProArg: 3.408 ± 0.437
3.288ProSer: 3.288 ± 0.47
4.603ProThr: 4.603 ± 0.674
3.946ProVal: 3.946 ± 0.434
1.375ProTrp: 1.375 ± 0.306
1.315ProTyr: 1.315 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
3.587GlnAla: 3.587 ± 0.481
0.418GlnCys: 0.418 ± 0.155
1.315GlnAsp: 1.315 ± 0.305
1.495GlnGlu: 1.495 ± 0.335
1.016GlnPhe: 1.016 ± 0.236
2.152GlnGly: 2.152 ± 0.361
1.076GlnHis: 1.076 ± 0.253
1.375GlnIle: 1.375 ± 0.273
1.076GlnLys: 1.076 ± 0.201
2.929GlnLeu: 2.929 ± 0.466
0.957GlnMet: 0.957 ± 0.236
0.957GlnAsn: 0.957 ± 0.208
2.81GlnPro: 2.81 ± 0.564
2.272GlnGln: 2.272 ± 0.465
2.87GlnArg: 2.87 ± 0.361
1.674GlnSer: 1.674 ± 0.367
1.853GlnThr: 1.853 ± 0.299
2.69GlnVal: 2.69 ± 0.444
0.837GlnTrp: 0.837 ± 0.223
1.375GlnTyr: 1.375 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
7.652ArgAla: 7.652 ± 0.768
0.598ArgCys: 0.598 ± 0.218
6.277ArgAsp: 6.277 ± 0.609
4.245ArgGlu: 4.245 ± 0.474
1.375ArgPhe: 1.375 ± 0.301
6.158ArgGly: 6.158 ± 0.579
2.63ArgHis: 2.63 ± 0.491
3.826ArgIle: 3.826 ± 0.492
2.033ArgLys: 2.033 ± 0.268
5.082ArgLeu: 5.082 ± 0.508
1.973ArgMet: 1.973 ± 0.38
2.511ArgAsn: 2.511 ± 0.391
3.109ArgPro: 3.109 ± 0.409
2.511ArgGln: 2.511 ± 0.422
7.114ArgArg: 7.114 ± 0.807
3.946ArgSer: 3.946 ± 0.492
4.544ArgThr: 4.544 ± 0.625
5.261ArgVal: 5.261 ± 0.59
1.794ArgTrp: 1.794 ± 0.339
1.614ArgTyr: 1.614 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
5.381SerAla: 5.381 ± 0.836
0.239SerCys: 0.239 ± 0.102
2.63SerAsp: 2.63 ± 0.506
3.228SerGlu: 3.228 ± 0.515
1.136SerPhe: 1.136 ± 0.321
6.397SerGly: 6.397 ± 0.792
0.897SerHis: 0.897 ± 0.206
3.408SerIle: 3.408 ± 0.428
1.375SerLys: 1.375 ± 0.287
3.169SerLeu: 3.169 ± 0.47
1.315SerMet: 1.315 ± 0.247
1.973SerAsn: 1.973 ± 0.424
2.571SerPro: 2.571 ± 0.379
1.315SerGln: 1.315 ± 0.308
3.408SerArg: 3.408 ± 0.439
2.152SerSer: 2.152 ± 0.394
3.707SerThr: 3.707 ± 0.544
3.886SerVal: 3.886 ± 0.473
1.554SerTrp: 1.554 ± 0.296
1.255SerTyr: 1.255 ± 0.355
0.0SerXaa: 0.0 ± 0.0
Thr
8.25ThrAla: 8.25 ± 0.873
1.016ThrCys: 1.016 ± 0.25
5.799ThrAsp: 5.799 ± 0.719
3.766ThrGlu: 3.766 ± 0.439
1.674ThrPhe: 1.674 ± 0.404
5.919ThrGly: 5.919 ± 0.635
1.495ThrHis: 1.495 ± 0.318
2.989ThrIle: 2.989 ± 0.4
2.391ThrLys: 2.391 ± 0.368
4.902ThrLeu: 4.902 ± 0.55
1.255ThrMet: 1.255 ± 0.248
2.391ThrAsn: 2.391 ± 0.474
4.603ThrPro: 4.603 ± 0.407
2.69ThrGln: 2.69 ± 0.413
4.364ThrArg: 4.364 ± 0.517
3.707ThrSer: 3.707 ± 0.416
4.125ThrThr: 4.125 ± 0.415
5.799ThrVal: 5.799 ± 0.643
1.136ThrTrp: 1.136 ± 0.264
1.196ThrTyr: 1.196 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
9.207ValAla: 9.207 ± 0.874
0.957ValCys: 0.957 ± 0.285
5.978ValAsp: 5.978 ± 0.661
5.022ValGlu: 5.022 ± 0.622
1.554ValPhe: 1.554 ± 0.313
6.217ValGly: 6.217 ± 0.665
1.554ValHis: 1.554 ± 0.321
3.408ValIle: 3.408 ± 0.435
2.212ValLys: 2.212 ± 0.437
4.842ValLeu: 4.842 ± 0.587
1.734ValMet: 1.734 ± 0.368
1.853ValAsn: 1.853 ± 0.318
3.707ValPro: 3.707 ± 0.472
2.571ValGln: 2.571 ± 0.461
5.799ValArg: 5.799 ± 0.719
2.87ValSer: 2.87 ± 0.471
5.978ValThr: 5.978 ± 0.77
7.234ValVal: 7.234 ± 0.7
1.674ValTrp: 1.674 ± 0.369
2.033ValTyr: 2.033 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.674TrpAla: 1.674 ± 0.286
0.179TrpCys: 0.179 ± 0.111
1.315TrpAsp: 1.315 ± 0.339
0.717TrpGlu: 0.717 ± 0.177
0.717TrpPhe: 0.717 ± 0.212
1.136TrpGly: 1.136 ± 0.239
0.538TrpHis: 0.538 ± 0.219
1.016TrpIle: 1.016 ± 0.249
0.538TrpLys: 0.538 ± 0.184
2.272TrpLeu: 2.272 ± 0.352
0.359TrpMet: 0.359 ± 0.147
0.957TrpAsn: 0.957 ± 0.323
1.315TrpPro: 1.315 ± 0.309
0.717TrpGln: 0.717 ± 0.216
1.853TrpArg: 1.853 ± 0.283
1.196TrpSer: 1.196 ± 0.296
1.435TrpThr: 1.435 ± 0.252
1.495TrpVal: 1.495 ± 0.296
0.717TrpTrp: 0.717 ± 0.239
0.837TrpTyr: 0.837 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.152TyrAla: 2.152 ± 0.298
0.12TyrCys: 0.12 ± 0.085
1.554TyrAsp: 1.554 ± 0.273
1.614TyrGlu: 1.614 ± 0.364
0.598TyrPhe: 0.598 ± 0.203
2.451TyrGly: 2.451 ± 0.391
0.837TyrHis: 0.837 ± 0.243
0.897TyrIle: 0.897 ± 0.216
1.016TyrLys: 1.016 ± 0.222
1.136TyrLeu: 1.136 ± 0.266
0.538TyrMet: 0.538 ± 0.179
0.658TyrAsn: 0.658 ± 0.172
1.554TyrPro: 1.554 ± 0.309
0.658TyrGln: 0.658 ± 0.224
1.853TyrArg: 1.853 ± 0.379
1.255TyrSer: 1.255 ± 0.246
1.674TyrThr: 1.674 ± 0.313
1.734TyrVal: 1.734 ± 0.396
0.478TyrTrp: 0.478 ± 0.158
0.538TyrTyr: 0.538 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16728 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski