Stenotrophomonas phage vB_SmaS-AXL_3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D4XWC9|A0A7D4XWC9_9CAUD DNA helicase OS=Stenotrophomonas phage vB_SmaS-AXL_3 OX=2740427 GN=AXL3_38 PE=4 SV=1
MM1 pKa = 7.1ARR3 pKa = 11.84AYY5 pKa = 9.63KK6 pKa = 10.52VEE8 pKa = 4.03ATNGAVRR15 pKa = 11.84YY16 pKa = 9.64AGSTTAARR24 pKa = 11.84AARR27 pKa = 11.84DD28 pKa = 3.74EE29 pKa = 4.78LLEE32 pKa = 4.0QYY34 pKa = 10.65PDD36 pKa = 3.49LRR38 pKa = 11.84KK39 pKa = 9.93KK40 pKa = 10.63DD41 pKa = 3.59VTIDD45 pKa = 3.65EE46 pKa = 4.37QVEE49 pKa = 4.27LPTKK53 pKa = 10.19KK54 pKa = 10.43DD55 pKa = 3.39EE56 pKa = 5.23LIEE59 pKa = 4.01FLNEE63 pKa = 3.6LLAEE67 pKa = 4.1YY68 pKa = 10.67DD69 pKa = 3.4PGEE72 pKa = 4.13EE73 pKa = 4.06EE74 pKa = 4.09EE75 pKa = 4.35EE76 pKa = 4.18EE77 pKa = 4.22EE78 pKa = 4.21EE79 pKa = 4.21EE80 pKa = 4.21EE81 pKa = 4.21EE82 pKa = 4.21EE83 pKa = 4.21EE84 pKa = 4.21EE85 pKa = 4.21EE86 pKa = 4.21EE87 pKa = 4.21EE88 pKa = 4.21EE89 pKa = 4.21EE90 pKa = 4.21EE91 pKa = 4.21EE92 pKa = 4.32EE93 pKa = 4.58EE94 pKa = 5.07EE95 pKa = 4.45EE96 pKa = 5.11EE97 pKa = 4.27EE98 pKa = 4.89DD99 pKa = 4.08

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5BFX7|A0A7D5BFX7_9CAUD Thymidylate synthase complementing protein OS=Stenotrophomonas phage vB_SmaS-AXL_3 OX=2740427 GN=AXL3_50 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.16RR3 pKa = 11.84RR4 pKa = 11.84GHH6 pKa = 5.55SLAGEE11 pKa = 3.98RR12 pKa = 11.84LFGKK16 pKa = 9.93RR17 pKa = 11.84NHH19 pKa = 7.1DD20 pKa = 3.24GTVRR24 pKa = 11.84HH25 pKa = 5.47MAEE28 pKa = 3.84GLRR31 pKa = 11.84CEE33 pKa = 5.63RR34 pKa = 11.84IRR36 pKa = 11.84KK37 pKa = 8.75WIFLGWTGPFNLIPRR52 pKa = 11.84WKK54 pKa = 9.37HH55 pKa = 4.8AVVGYY60 pKa = 10.42RR61 pKa = 11.84YY62 pKa = 9.63FDD64 pKa = 3.43EE65 pKa = 4.3NGRR68 pKa = 11.84RR69 pKa = 11.84VLMPWEE75 pKa = 4.51TKK77 pKa = 10.67HH78 pKa = 6.58EE79 pKa = 4.21DD80 pKa = 3.06

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14806

30

1036

227.8

25.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.577 ± 0.413

0.952 ± 0.125

6.464 ± 0.224

6.464 ± 0.41

3.634 ± 0.18

7.389 ± 0.272

1.763 ± 0.177

4.356 ± 0.179

6.099 ± 0.453

8.902 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.128

4.127 ± 0.185

5.011 ± 0.232

3.931 ± 0.252

6.126 ± 0.229

5.106 ± 0.222

5.944 ± 0.239

6.997 ± 0.249

1.709 ± 0.141

3.093 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski