Campylobacter curvus (strain 525.92)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1860 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7GY14|A7GY14_CAMC5 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase OS=Campylobacter curvus (strain 525.92) OX=360105 GN=CCV52592_1631 PE=3 SV=1
MM1 pKa = 7.6 TYY3 pKa = 10.93 DD4 pKa = 3.86 EE5 pKa = 5.47 LEE7 pKa = 4.27 LDD9 pKa = 3.7 AVLLEE14 pKa = 4.07 VLEE17 pKa = 4.31 NRR19 pKa = 11.84 GSFDD23 pKa = 4.22 SMDD26 pKa = 3.98 DD27 pKa = 3.51 EE28 pKa = 4.42 EE29 pKa = 5.98 LYY31 pKa = 11.1 EE32 pKa = 5.02 LIEE35 pKa = 4.76 QIAQYY40 pKa = 10.85 TDD42 pKa = 2.91 GDD44 pKa = 3.9 YY45 pKa = 11.68 EE46 pKa = 4.12 EE47 pKa = 5.53 AFEE50 pKa = 4.95 YY51 pKa = 7.58 MTQFSPIPKK60 pKa = 9.43 KK61 pKa = 10.64 RR62 pKa = 11.84 FVSLFMVV69 pKa = 4.15
Molecular weight: 8.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.732
IPC_protein 3.63
Toseland 3.465
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.528
Rodwell 3.478
Grimsley 3.376
Solomon 3.579
Lehninger 3.541
Nozaki 3.745
DTASelect 3.872
Thurlkill 3.516
EMBOSS 3.541
Sillero 3.757
Patrickios 1.812
IPC_peptide 3.592
IPC2_peptide 3.732
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A7GYZ9|A7GYZ9_CAMC5 Hypothetical membrane protein (DUF3815 domain) OS=Campylobacter curvus (strain 525.92) OX=360105 GN=CCV52592_1825 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 5.76 KK9 pKa = 7.92 TPKK12 pKa = 9.96 KK13 pKa = 7.49 RR14 pKa = 11.84 THH16 pKa = 5.99 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MKK23 pKa = 8.96 TKK25 pKa = 10.43 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAAA44 pKa = 4.42
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1860
0
1860
586621
37
1787
315.4
35.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.293 ± 0.058
1.139 ± 0.024
5.605 ± 0.044
6.567 ± 0.06
5.462 ± 0.05
6.654 ± 0.051
1.562 ± 0.024
7.954 ± 0.051
8.171 ± 0.054
9.767 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.498 ± 0.027
5.178 ± 0.043
2.996 ± 0.032
2.874 ± 0.027
3.868 ± 0.035
6.552 ± 0.043
4.319 ± 0.052
6.353 ± 0.043
0.722 ± 0.018
3.467 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here