Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060YHS8|A0A060YHS8_ONCMY DUF4371 domain-containing protein OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00057100001 PE=4 SV=1
II1 pKa = 7.73LVDD4 pKa = 4.23LKK6 pKa = 11.32QEE8 pKa = 3.46VWYY11 pKa = 9.57QKK13 pKa = 10.78CHH15 pKa = 7.14DD16 pKa = 4.39PACKK20 pKa = 10.08SFRR23 pKa = 11.84SSSYY27 pKa = 10.26PLPQDD32 pKa = 2.6ICVSYY37 pKa = 10.48LITMDD42 pKa = 4.95EE43 pKa = 4.0EE44 pKa = 4.22DD45 pKa = 3.43QAYY48 pKa = 10.96LMDD51 pKa = 4.76DD52 pKa = 3.35VGNIEE57 pKa = 4.84LSQTLPVEE65 pKa = 4.46KK66 pKa = 10.74YY67 pKa = 10.41LGGSAATLPQEE78 pKa = 4.61EE79 pKa = 5.3DD80 pKa = 3.28RR81 pKa = 11.84DD82 pKa = 3.9PWGDD86 pKa = 3.43DD87 pKa = 3.26QAYY90 pKa = 10.7LEE92 pKa = 4.35ALEE95 pKa = 4.48DD96 pKa = 4.01VEE98 pKa = 6.27RR99 pKa = 11.84STGDD103 pKa = 3.35LAEE106 pKa = 5.45DD107 pKa = 3.96DD108 pKa = 4.71VPDD111 pKa = 3.96EE112 pKa = 5.08LLLQTMTEE120 pKa = 4.18FEE122 pKa = 4.39SLKK125 pKa = 10.6DD126 pKa = 3.55LCLSATKK133 pKa = 10.5CC134 pKa = 3.64

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060Y2U7|A0A060Y2U7_ONCMY G_PROTEIN_RECEP_F3_4 domain-containing protein OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00054778001 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.41NALDD6 pKa = 4.29KK7 pKa = 11.57LDD9 pKa = 3.9GTDD12 pKa = 3.7LNGRR16 pKa = 11.84KK17 pKa = 9.29LKK19 pKa = 10.76LSEE22 pKa = 4.0DD23 pKa = 3.52RR24 pKa = 11.84KK25 pKa = 9.85SRR27 pKa = 11.84RR28 pKa = 11.84SRR30 pKa = 11.84SRR32 pKa = 11.84SRR34 pKa = 11.84GSRR37 pKa = 11.84SYY39 pKa = 10.67SRR41 pKa = 11.84SRR43 pKa = 11.84SRR45 pKa = 11.84SRR47 pKa = 11.84SPRR50 pKa = 11.84SKK52 pKa = 10.2SPSRR56 pKa = 11.84SRR58 pKa = 11.84SRR60 pKa = 11.84NRR62 pKa = 11.84SRR64 pKa = 11.84SPRR67 pKa = 11.84SASRR71 pKa = 11.84TPEE74 pKa = 4.06KK75 pKa = 10.52KK76 pKa = 9.73PSSGGGGGRR85 pKa = 11.84AAHH88 pKa = 6.98RR89 pKa = 11.84STSRR93 pKa = 11.84SPSRR97 pKa = 11.84SKK99 pKa = 10.78SRR101 pKa = 11.84SRR103 pKa = 11.84TPPARR108 pKa = 11.84SRR110 pKa = 11.84SPAPRR115 pKa = 11.84SRR117 pKa = 11.84TPPPRR122 pKa = 11.84SRR124 pKa = 11.84SPAPRR129 pKa = 11.84SRR131 pKa = 11.84TPPPRR136 pKa = 11.84SRR138 pKa = 11.84SPAPARR144 pKa = 11.84KK145 pKa = 8.63PSRR148 pKa = 11.84SRR150 pKa = 11.84SPSVEE155 pKa = 4.02SQHH158 pKa = 6.64

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46405

42

46447

17804350

19

20979

383.3

42.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.21 ± 0.011

2.39 ± 0.009

5.034 ± 0.008

6.647 ± 0.019

3.558 ± 0.009

6.489 ± 0.015

2.815 ± 0.007

4.323 ± 0.009

5.487 ± 0.015

9.593 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.006

3.811 ± 0.007

5.831 ± 0.015

4.688 ± 0.014

5.662 ± 0.01

8.647 ± 0.015

5.891 ± 0.012

6.418 ± 0.012

1.169 ± 0.004

2.773 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski