Amino acid dipepetide frequency for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.216AlaAla: 5.216 ± 0.027	1.31AlaCys: 1.31 ± 0.009	2.854AlaAsp: 2.854 ± 0.016	4.151AlaGlu: 4.151 ± 0.02	2.267AlaPhe: 2.267 ± 0.012	4.152AlaGly: 4.152 ± 0.021	1.489AlaHis: 1.489 ± 0.009	2.636AlaIle: 2.636 ± 0.014	3.262AlaLys: 3.262 ± 0.017	6.24AlaLeu: 6.24 ± 0.028	1.656AlaMet: 1.656 ± 0.01	2.119AlaAsn: 2.119 ± 0.011	3.452AlaPro: 3.452 ± 0.021	2.827AlaGln: 2.827 ± 0.017	3.1AlaArg: 3.1 ± 0.015	5.14AlaSer: 5.14 ± 0.02	3.551AlaThr: 3.551 ± 0.015	4.56AlaVal: 4.56 ± 0.017	0.659AlaTrp: 0.659 ± 0.007	1.456AlaTyr: 1.456 ± 0.009	0.007AlaXaa: 0.007 ± 0.001
Cys	1.172CysAla: 1.172 ± 0.011	0.728CysCys: 0.728 ± 0.009	1.128CysAsp: 1.128 ± 0.011	1.24CysGlu: 1.24 ± 0.011	0.967CysPhe: 0.967 ± 0.008	1.613CysGly: 1.613 ± 0.014	0.715CysHis: 0.715 ± 0.008	1.002CysIle: 1.002 ± 0.009	1.167CysLys: 1.167 ± 0.009	2.387CysLeu: 2.387 ± 0.015	0.549CysMet: 0.549 ± 0.006	0.874CysAsn: 0.874 ± 0.008	1.39CysPro: 1.39 ± 0.013	1.056CysGln: 1.056 ± 0.01	1.301CysArg: 1.301 ± 0.01	2.207CysSer: 2.207 ± 0.015	1.265CysThr: 1.265 ± 0.011	2.166CysVal: 2.166 ± 0.026	0.304CysTrp: 0.304 ± 0.004	0.665CysTyr: 0.665 ± 0.006	0.006CysXaa: 0.006 ± 0.001
Asp	2.633AspAla: 2.633 ± 0.013	1.171AspCys: 1.171 ± 0.01	2.894AspAsp: 2.894 ± 0.019	3.415AspGlu: 3.415 ± 0.02	1.96AspPhe: 1.96 ± 0.012	3.421AspGly: 3.421 ± 0.022	1.237AspHis: 1.237 ± 0.009	2.657AspIle: 2.657 ± 0.014	2.624AspLys: 2.624 ± 0.014	4.863AspLeu: 4.863 ± 0.019	1.379AspMet: 1.379 ± 0.01	2.035AspAsn: 2.035 ± 0.013	2.856AspPro: 2.856 ± 0.015	2.009AspGln: 2.009 ± 0.013	2.782AspArg: 2.782 ± 0.015	4.316AspSer: 4.316 ± 0.017	2.802AspThr: 2.802 ± 0.014	3.071AspVal: 3.071 ± 0.018	0.69AspTrp: 0.69 ± 0.006	1.523AspTyr: 1.523 ± 0.011	0.003AspXaa: 0.003 ± 0.0
Glu	4.32GluAla: 4.32 ± 0.022	1.212GluCys: 1.212 ± 0.013	4.219GluAsp: 4.219 ± 0.022	7.487GluGlu: 7.487 ± 0.048	1.84GluPhe: 1.84 ± 0.011	4.441GluGly: 4.441 ± 0.019	1.445GluHis: 1.445 ± 0.01	2.698GluIle: 2.698 ± 0.014	4.414GluLys: 4.414 ± 0.028	5.814GluLeu: 5.814 ± 0.03	1.776GluMet: 1.776 ± 0.011	2.569GluAsn: 2.569 ± 0.017	2.728GluPro: 2.728 ± 0.018	2.895GluGln: 2.895 ± 0.021	4.419GluArg: 4.419 ± 0.027	4.185GluSer: 4.185 ± 0.019	3.406GluThr: 3.406 ± 0.02	4.341GluVal: 4.341 ± 0.019	0.715GluTrp: 0.715 ± 0.007	1.542GluTyr: 1.542 ± 0.011	0.004GluXaa: 0.004 ± 0.001
Phe	1.789PheAla: 1.789 ± 0.011	0.978PheCys: 0.978 ± 0.009	1.651PheAsp: 1.651 ± 0.011	1.73PheGlu: 1.73 ± 0.011	1.564PhePhe: 1.564 ± 0.013	2.14PheGly: 2.14 ± 0.015	1.051PheHis: 1.051 ± 0.009	1.831PheIle: 1.831 ± 0.014	1.762PheLys: 1.762 ± 0.011	3.874PheLeu: 3.874 ± 0.02	0.833PheMet: 0.833 ± 0.007	1.485PheAsn: 1.485 ± 0.01	1.87PhePro: 1.87 ± 0.013	1.663PheGln: 1.663 ± 0.011	1.862PheArg: 1.862 ± 0.015	3.468PheSer: 3.468 ± 0.015	2.256PheThr: 2.256 ± 0.011	2.127PheVal: 2.127 ± 0.014	0.452PheTrp: 0.452 ± 0.006	1.186PheTyr: 1.186 ± 0.009	0.005PheXaa: 0.005 ± 0.001
Gly	3.931GlyAla: 3.931 ± 0.019	1.345GlyCys: 1.345 ± 0.01	3.326GlyAsp: 3.326 ± 0.02	4.394GlyGlu: 4.394 ± 0.022	2.321GlyPhe: 2.321 ± 0.015	5.759GlyGly: 5.759 ± 0.034	1.8GlyHis: 1.8 ± 0.013	2.506GlyIle: 2.506 ± 0.013	3.691GlyLys: 3.691 ± 0.019	5.634GlyLeu: 5.634 ± 0.022	1.66GlyMet: 1.66 ± 0.012	2.494GlyAsn: 2.494 ± 0.015	3.396GlyPro: 3.396 ± 0.033	2.915GlyGln: 2.915 ± 0.018	3.742GlyArg: 3.742 ± 0.016	5.69GlySer: 5.69 ± 0.025	3.522GlyThr: 3.522 ± 0.018	4.085GlyVal: 4.085 ± 0.016	0.796GlyTrp: 0.796 ± 0.008	1.87GlyTyr: 1.87 ± 0.015	0.009GlyXaa: 0.009 ± 0.001
His	1.329HisAla: 1.329 ± 0.01	0.848HisCys: 0.848 ± 0.008	0.991HisAsp: 0.991 ± 0.008	1.147HisGlu: 1.147 ± 0.009	1.089HisPhe: 1.089 ± 0.008	1.63HisGly: 1.63 ± 0.013	1.183HisHis: 1.183 ± 0.012	1.37HisIle: 1.37 ± 0.009	1.304HisLys: 1.304 ± 0.009	2.854HisLeu: 2.854 ± 0.015	0.74HisMet: 0.74 ± 0.007	1.119HisAsn: 1.119 ± 0.009	1.734HisPro: 1.734 ± 0.014	1.397HisGln: 1.397 ± 0.012	1.71HisArg: 1.71 ± 0.011	2.661HisSer: 2.661 ± 0.015	2.31HisThr: 2.31 ± 0.024	1.481HisVal: 1.481 ± 0.011	0.353HisTrp: 0.353 ± 0.005	0.898HisTyr: 0.898 ± 0.008	0.005HisXaa: 0.005 ± 0.001
Ile	2.435IleAla: 2.435 ± 0.015	1.068IleCys: 1.068 ± 0.009	2.066IleAsp: 2.066 ± 0.014	2.365IleGlu: 2.365 ± 0.015	1.724IlePhe: 1.724 ± 0.012	2.287IleGly: 2.287 ± 0.012	1.346IleHis: 1.346 ± 0.01	2.314IleIle: 2.314 ± 0.013	2.447IleLys: 2.447 ± 0.014	4.302IleLeu: 4.302 ± 0.018	1.111IleMet: 1.111 ± 0.009	1.878IleAsn: 1.878 ± 0.012	2.519IlePro: 2.519 ± 0.014	2.115IleGln: 2.115 ± 0.011	2.322IleArg: 2.322 ± 0.012	3.669IleSer: 3.669 ± 0.015	2.829IleThr: 2.829 ± 0.018	2.559IleVal: 2.559 ± 0.015	0.48IleTrp: 0.48 ± 0.006	1.386IleTyr: 1.386 ± 0.01	0.006IleXaa: 0.006 ± 0.001
Lys	3.632LysAla: 3.632 ± 0.02	1.078LysCys: 1.078 ± 0.01	3.063LysAsp: 3.063 ± 0.016	4.484LysGlu: 4.484 ± 0.027	1.494LysPhe: 1.494 ± 0.01	3.278LysGly: 3.278 ± 0.019	1.472LysHis: 1.472 ± 0.011	2.393LysIle: 2.393 ± 0.015	4.178LysLys: 4.178 ± 0.025	4.745LysLeu: 4.745 ± 0.02	1.542LysMet: 1.542 ± 0.01	2.114LysAsn: 2.114 ± 0.012	2.928LysPro: 2.928 ± 0.021	2.435LysGln: 2.435 ± 0.014	3.501LysArg: 3.501 ± 0.017	3.702LysSer: 3.702 ± 0.019	3.198LysThr: 3.198 ± 0.016	3.555LysVal: 3.555 ± 0.017	0.593LysTrp: 0.593 ± 0.007	1.476LysTyr: 1.476 ± 0.011	0.005LysXaa: 0.005 ± 0.001
Leu	5.915LeuAla: 5.915 ± 0.022	2.44LeuCys: 2.44 ± 0.014	4.735LeuAsp: 4.735 ± 0.02	6.194LeuGlu: 6.194 ± 0.029	3.527LeuPhe: 3.527 ± 0.019	5.388LeuGly: 5.388 ± 0.02	2.803LeuHis: 2.803 ± 0.015	3.686LeuIle: 3.686 ± 0.017	5.473LeuLys: 5.473 ± 0.023	9.938LeuLeu: 9.938 ± 0.044	2.135LeuMet: 2.135 ± 0.012	3.638LeuAsn: 3.638 ± 0.017	5.52LeuPro: 5.52 ± 0.023	5.273LeuGln: 5.273 ± 0.027	5.479LeuArg: 5.479 ± 0.026	9.008LeuSer: 9.008 ± 0.029	5.438LeuThr: 5.438 ± 0.02	5.533LeuVal: 5.533 ± 0.025	1.102LeuTrp: 1.102 ± 0.009	2.688LeuTyr: 2.688 ± 0.013	0.017LeuXaa: 0.017 ± 0.001
Met	2.034MetAla: 2.034 ± 0.011	0.55MetCys: 0.55 ± 0.006	1.529MetAsp: 1.529 ± 0.01	2.094MetGlu: 2.094 ± 0.012	0.919MetPhe: 0.919 ± 0.008	1.636MetGly: 1.636 ± 0.013	0.56MetHis: 0.56 ± 0.005	0.904MetIle: 0.904 ± 0.007	1.456MetLys: 1.456 ± 0.009	2.136MetLeu: 2.136 ± 0.011	0.75MetMet: 0.75 ± 0.008	0.919MetAsn: 0.919 ± 0.008	1.176MetPro: 1.176 ± 0.01	1.015MetGln: 1.015 ± 0.008	1.271MetArg: 1.271 ± 0.009	2.0MetSer: 2.0 ± 0.012	1.397MetThr: 1.397 ± 0.01	1.713MetVal: 1.713 ± 0.01	0.28MetTrp: 0.28 ± 0.004	0.703MetTyr: 0.703 ± 0.006	0.002MetXaa: 0.002 ± 0.0
Asn	2.083AsnAla: 2.083 ± 0.012	0.875AsnCys: 0.875 ± 0.008	1.692AsnAsp: 1.692 ± 0.011	1.967AsnGlu: 1.967 ± 0.012	1.325AsnPhe: 1.325 ± 0.012	2.737AsnGly: 2.737 ± 0.016	1.067AsnHis: 1.067 ± 0.01	2.117AsnIle: 2.117 ± 0.015	2.159AsnLys: 2.159 ± 0.012	3.588AsnLeu: 3.588 ± 0.016	1.098AsnMet: 1.098 ± 0.008	1.829AsnAsn: 1.829 ± 0.012	2.29AsnPro: 2.29 ± 0.014	1.711AsnGln: 1.711 ± 0.01	2.015AsnArg: 2.015 ± 0.011	3.172AsnSer: 3.172 ± 0.017	2.451AsnThr: 2.451 ± 0.011	2.345AsnVal: 2.345 ± 0.012	0.445AsnTrp: 0.445 ± 0.005	1.137AsnTyr: 1.137 ± 0.009	0.004AsnXaa: 0.004 ± 0.0
Pro	3.863ProAla: 3.863 ± 0.021	1.168ProCys: 1.168 ± 0.01	2.805ProAsp: 2.805 ± 0.013	3.675ProGlu: 3.675 ± 0.019	1.843ProPhe: 1.843 ± 0.012	4.211ProGly: 4.211 ± 0.041	1.622ProHis: 1.622 ± 0.013	2.05ProIle: 2.05 ± 0.013	2.614ProLys: 2.614 ± 0.018	5.204ProLeu: 5.204 ± 0.025	1.208ProMet: 1.208 ± 0.009	1.978ProAsn: 1.978 ± 0.013	5.392ProPro: 5.392 ± 0.041	2.787ProGln: 2.787 ± 0.02	2.864ProArg: 2.864 ± 0.016	5.801ProSer: 5.801 ± 0.03	3.396ProThr: 3.396 ± 0.019	3.739ProVal: 3.739 ± 0.02	0.576ProTrp: 0.576 ± 0.007	1.506ProTyr: 1.506 ± 0.012	0.01ProXaa: 0.01 ± 0.001
Gln	3.161GlnAla: 3.161 ± 0.017	1.077GlnCys: 1.077 ± 0.012	2.314GlnAsp: 2.314 ± 0.014	3.377GlnGlu: 3.377 ± 0.023	1.314GlnPhe: 1.314 ± 0.009	3.006GlnGly: 3.006 ± 0.02	1.465GlnHis: 1.465 ± 0.012	1.829GlnIle: 1.829 ± 0.012	2.396GlnLys: 2.396 ± 0.013	4.338GlnLeu: 4.338 ± 0.025	1.143GlnMet: 1.143 ± 0.009	1.743GlnAsn: 1.743 ± 0.012	2.633GlnPro: 2.633 ± 0.017	3.176GlnGln: 3.176 ± 0.033	3.104GlnArg: 3.104 ± 0.018	3.533GlnSer: 3.533 ± 0.018	2.642GlnThr: 2.642 ± 0.016	2.836GlnVal: 2.836 ± 0.014	0.543GlnTrp: 0.543 ± 0.006	1.245GlnTyr: 1.245 ± 0.009	0.004GlnXaa: 0.004 ± 0.0
Arg	3.394ArgAla: 3.394 ± 0.017	1.26ArgCys: 1.26 ± 0.011	2.952ArgAsp: 2.952 ± 0.016	4.038ArgGlu: 4.038 ± 0.022	1.893ArgPhe: 1.893 ± 0.009	3.727ArgGly: 3.727 ± 0.021	1.665ArgHis: 1.665 ± 0.01	2.291ArgIle: 2.291 ± 0.013	3.515ArgLys: 3.515 ± 0.016	5.221ArgLeu: 5.221 ± 0.022	1.428ArgMet: 1.428 ± 0.01	2.084ArgAsn: 2.084 ± 0.011	3.064ArgPro: 3.064 ± 0.016	2.662ArgGln: 2.662 ± 0.017	4.312ArgArg: 4.312 ± 0.023	4.439ArgSer: 4.439 ± 0.024	2.998ArgThr: 2.998 ± 0.015	3.438ArgVal: 3.438 ± 0.018	0.661ArgTrp: 0.661 ± 0.007	1.567ArgTyr: 1.567 ± 0.01	0.009ArgXaa: 0.009 ± 0.001
Ser	5.141SerAla: 5.141 ± 0.022	1.987SerCys: 1.987 ± 0.015	4.151SerAsp: 4.151 ± 0.018	4.704SerGlu: 4.704 ± 0.02	3.115SerPhe: 3.115 ± 0.017	5.567SerGly: 5.567 ± 0.021	2.4SerHis: 2.4 ± 0.013	3.431SerIle: 3.431 ± 0.014	3.899SerLys: 3.899 ± 0.018	9.041SerLeu: 9.041 ± 0.031	1.994SerMet: 1.994 ± 0.011	3.019SerAsn: 3.019 ± 0.014	6.218SerPro: 6.218 ± 0.038	3.914SerGln: 3.914 ± 0.022	4.414SerArg: 4.414 ± 0.021	9.745SerSer: 9.745 ± 0.051	5.079SerThr: 5.079 ± 0.02	5.477SerVal: 5.477 ± 0.019	0.961SerTrp: 0.961 ± 0.008	2.201SerTyr: 2.201 ± 0.012	0.014SerXaa: 0.014 ± 0.001
Thr	4.004ThrAla: 4.004 ± 0.016	1.387ThrCys: 1.387 ± 0.013	2.904ThrAsp: 2.904 ± 0.014	3.754ThrGlu: 3.754 ± 0.018	2.119ThrPhe: 2.119 ± 0.014	3.932ThrGly: 3.932 ± 0.017	2.063ThrHis: 2.063 ± 0.022	2.551ThrIle: 2.551 ± 0.014	2.75ThrLys: 2.75 ± 0.018	5.663ThrLeu: 5.663 ± 0.02	1.381ThrMet: 1.381 ± 0.008	2.025ThrAsn: 2.025 ± 0.01	3.897ThrPro: 3.897 ± 0.023	2.574ThrGln: 2.574 ± 0.015	2.61ThrArg: 2.61 ± 0.014	4.93ThrSer: 4.93 ± 0.025	3.863ThrThr: 3.863 ± 0.029	4.33ThrVal: 4.33 ± 0.02	0.676ThrTrp: 0.676 ± 0.007	1.48ThrTyr: 1.48 ± 0.009	0.014ThrXaa: 0.014 ± 0.001
Val	4.004ValAla: 4.004 ± 0.017	2.349ValCys: 2.349 ± 0.026	3.103ValAsp: 3.103 ± 0.014	4.054ValGlu: 4.054 ± 0.017	2.639ValPhe: 2.639 ± 0.013	3.587ValGly: 3.587 ± 0.017	1.632ValHis: 1.632 ± 0.011	2.943ValIle: 2.943 ± 0.017	3.612ValLys: 3.612 ± 0.018	6.201ValLeu: 6.201 ± 0.022	1.59ValMet: 1.59 ± 0.012	2.46ValAsn: 2.46 ± 0.014	3.353ValPro: 3.353 ± 0.017	2.716ValGln: 2.716 ± 0.015	3.306ValArg: 3.306 ± 0.013	5.391ValSer: 5.391 ± 0.019	4.055ValThr: 4.055 ± 0.023	4.48ValVal: 4.48 ± 0.024	0.832ValTrp: 0.832 ± 0.007	1.861ValTyr: 1.861 ± 0.012	0.013ValXaa: 0.013 ± 0.001
Trp	0.685TrpAla: 0.685 ± 0.007	0.274TrpCys: 0.274 ± 0.004	0.635TrpAsp: 0.635 ± 0.006	0.726TrpGlu: 0.726 ± 0.008	0.464TrpPhe: 0.464 ± 0.005	0.693TrpGly: 0.693 ± 0.007	0.284TrpHis: 0.284 ± 0.005	0.528TrpIle: 0.528 ± 0.006	0.703TrpLys: 0.703 ± 0.007	1.16TrpLeu: 1.16 ± 0.01	0.356TrpMet: 0.356 ± 0.005	0.512TrpAsn: 0.512 ± 0.006	0.484TrpPro: 0.484 ± 0.005	0.471TrpGln: 0.471 ± 0.006	0.791TrpArg: 0.791 ± 0.007	0.941TrpSer: 0.941 ± 0.008	0.705TrpThr: 0.705 ± 0.009	0.741TrpVal: 0.741 ± 0.007	0.188TrpTrp: 0.188 ± 0.003	0.346TrpTyr: 0.346 ± 0.005	0.002TrpXaa: 0.002 ± 0.0
Tyr	1.359TyrAla: 1.359 ± 0.01	0.79TyrCys: 0.79 ± 0.008	1.327TyrAsp: 1.327 ± 0.011	1.47TyrGlu: 1.47 ± 0.01	1.184TyrPhe: 1.184 ± 0.01	1.676TyrGly: 1.676 ± 0.01	0.848TyrHis: 0.848 ± 0.008	1.483TyrIle: 1.483 ± 0.012	1.437TyrLys: 1.437 ± 0.01	2.707TyrLeu: 2.707 ± 0.015	0.711TyrMet: 0.711 ± 0.006	1.208TyrAsn: 1.208 ± 0.007	1.4TyrPro: 1.4 ± 0.011	1.265TyrGln: 1.265 ± 0.01	1.701TyrArg: 1.701 ± 0.011	2.46TyrSer: 2.46 ± 0.013	1.734TyrThr: 1.734 ± 0.012	1.595TyrVal: 1.595 ± 0.011	0.383TyrTrp: 0.383 ± 0.007	0.987TyrTyr: 0.987 ± 0.01	0.004TyrXaa: 0.004 ± 0.001
Xaa	0.005XaaAla: 0.005 ± 0.001	0.007XaaCys: 0.007 ± 0.001	0.004XaaAsp: 0.004 ± 0.0	0.005XaaGlu: 0.005 ± 0.0	0.006XaaPhe: 0.006 ± 0.001	0.007XaaGly: 0.007 ± 0.001	0.005XaaHis: 0.005 ± 0.001	0.005XaaIle: 0.005 ± 0.001	0.005XaaLys: 0.005 ± 0.001	0.018XaaLeu: 0.018 ± 0.001	0.005XaaMet: 0.005 ± 0.0	0.004XaaAsn: 0.004 ± 0.001	0.008XaaPro: 0.008 ± 0.001	0.004XaaGln: 0.004 ± 0.0	0.01XaaArg: 0.01 ± 0.001	0.016XaaSer: 0.016 ± 0.001	0.01XaaThr: 0.01 ± 0.001	0.011XaaVal: 0.011 ± 0.001	0.002XaaTrp: 0.002 ± 0.0	0.005XaaTyr: 0.005 ± 0.001	0.466XaaXaa: 0.466 ± 0.175

Statistics based on 46447 proteins (17804350 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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