Acidibacillus sulfuroxidans
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2818 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U3D7Q1|A0A2U3D7Q1_9BACL DksA C4-type domain-containing protein OS=Acidibacillus sulfuroxidans OX=1765684 GN=BM613_08935 PE=4 SV=1
MM1 pKa = 7.75 SINLLQQLNSIPNLGGTGLPVQQYY25 pKa = 10.09 ASEE28 pKa = 4.11 MQQVLTAQFEE38 pKa = 4.74 TIPNSEE44 pKa = 4.85 LSTLSTTQNALNSLQPALQQLQSATQTLASSLSWNAVHH82 pKa = 7.06 LTTTGSGFTATAQAGAEE99 pKa = 4.03 PSSYY103 pKa = 10.16 TIQVNNLATAQWNTASVSLATSTGSSTLTAGTFAITPSSTSQLTGTATITVTSGEE158 pKa = 4.36 SLSDD162 pKa = 2.8 IAAAVNQDD170 pKa = 3.2 SAATGVVASIIQSNNGYY187 pKa = 9.3 QLTFQDD193 pKa = 4.36 VNPGATNTFSLTDD206 pKa = 3.14 VSGNVISSQLKK217 pKa = 7.43 VTQTATATDD226 pKa = 3.56 ASITLDD232 pKa = 2.94 GSMTLTSSSDD242 pKa = 3.14 TFANAIPNVTINVNQAGASGTILLAQDD269 pKa = 3.6 SSAALSAVQNWMNAYY284 pKa = 10.13 NSVISTLHH292 pKa = 6.41 TDD294 pKa = 3.45 TAYY297 pKa = 9.88 TPASASNPAAGAQTGPLFTDD317 pKa = 4.56 PAATGLLAQLPNSVNSFIGASGATGSSNFYY347 pKa = 10.58 HH348 pKa = 6.92 SLSQLGIVVDD358 pKa = 4.26 PNTGQLEE365 pKa = 4.26 FQSASGFAANPASGTAGFSGTLQSGQAMFTHH396 pKa = 7.32 AVQSNLAAVQQLFGVVQTSSLSSEE420 pKa = 4.26 VPTNGILGNLNTVLNEE436 pKa = 3.81 FLGSGSSTGVISGEE450 pKa = 4.03 LQSISQQQKK459 pKa = 10.33 EE460 pKa = 4.01 INQYY464 pKa = 10.35 LKK466 pKa = 10.47 QVNQMITNNVQNFTSQLNNLNASLQQSSAQMQQINAMLNGASSSSSSSSSNSSGGG521 pKa = 3.3
Molecular weight: 52.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.923
Patrickios 0.426
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A2U3D6C3|A0A2U3D6C3_9BACL Uncharacterized protein OS=Acidibacillus sulfuroxidans OX=1765684 GN=BM613_11805 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 8.75 PTFNPNVRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.86 KK14 pKa = 9.56 VHH16 pKa = 5.96 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MSTKK25 pKa = 9.43 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.78 GRR39 pKa = 11.84 RR40 pKa = 11.84 VISAA44 pKa = 3.77
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2818
0
2818
825953
26
1525
293.1
32.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.139 ± 0.053
0.757 ± 0.014
4.732 ± 0.035
6.36 ± 0.05
4.129 ± 0.04
7.176 ± 0.047
2.536 ± 0.024
6.4 ± 0.036
4.263 ± 0.037
10.57 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.591 ± 0.019
3.064 ± 0.037
4.222 ± 0.03
4.492 ± 0.033
5.913 ± 0.05
5.884 ± 0.037
5.615 ± 0.03
7.859 ± 0.036
1.208 ± 0.021
3.09 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here