Kribbella amoyensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A561BT07|A0A561BT07_9ACTN Assimilatory nitrate reductase (NADH) alpha subunit apoprotein OS=Kribbella amoyensis OX=996641 GN=FB561_3154 PE=4 SV=1
MM1 pKa = 6.94FRR3 pKa = 11.84KK4 pKa = 9.75RR5 pKa = 11.84VAAAVMISGLAVATFGAINPASASEE30 pKa = 4.13SAPAAGSVRR39 pKa = 11.84MWEE42 pKa = 4.18DD43 pKa = 3.12PNYY46 pKa = 10.33TGSQYY51 pKa = 11.24VDD53 pKa = 3.36ATPANCPDD61 pKa = 3.45GCDD64 pKa = 3.23IDD66 pKa = 5.38GWDD69 pKa = 4.09GDD71 pKa = 4.15NEE73 pKa = 4.04ISSVRR78 pKa = 11.84NEE80 pKa = 4.09TTCTVRR86 pKa = 11.84LYY88 pKa = 11.21ADD90 pKa = 3.87DD91 pKa = 4.25NFGGRR96 pKa = 11.84SVDD99 pKa = 3.63LAAGEE104 pKa = 4.67SYY106 pKa = 11.63SNLEE110 pKa = 4.31LVGFDD115 pKa = 3.59NDD117 pKa = 3.59AEE119 pKa = 4.41SFQFVCC125 pKa = 5.56

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A561BS86|A0A561BS86_9ACTN Nucleotide-binding universal stress UspA family protein OS=Kribbella amoyensis OX=996641 GN=FB561_2882 PE=3 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84PHH5 pKa = 5.96RR6 pKa = 11.84AGRR9 pKa = 11.84ASRR12 pKa = 11.84AVGGPSPPTGRR23 pKa = 11.84RR24 pKa = 11.84STCAPNRR31 pKa = 11.84PRR33 pKa = 11.84PARR36 pKa = 11.84APPRR40 pKa = 11.84TSRR43 pKa = 11.84PGTSRR48 pKa = 11.84PHH50 pKa = 6.21PRR52 pKa = 11.84RR53 pKa = 11.84NTSPVARR60 pKa = 11.84AQPVRR65 pKa = 11.84RR66 pKa = 11.84ARR68 pKa = 11.84RR69 pKa = 11.84VRR71 pKa = 11.84VVRR74 pKa = 11.84LVRR77 pKa = 11.84VRR79 pKa = 11.84RR80 pKa = 11.84VGSPRR85 pKa = 11.84GSPARR90 pKa = 11.84RR91 pKa = 11.84TTAPPVRR98 pKa = 11.84RR99 pKa = 11.84ARR101 pKa = 11.84ARR103 pKa = 11.84ARR105 pKa = 11.84ARR107 pKa = 11.84ARR109 pKa = 11.84ARR111 pKa = 11.84ARR113 pKa = 11.84ARR115 pKa = 11.84ARR117 pKa = 11.84APQVSRR123 pKa = 11.84APRR126 pKa = 11.84PRR128 pKa = 11.84QVPAGRR134 pKa = 11.84VRR136 pKa = 11.84QVPPATTLPPAPPARR151 pKa = 11.84ADD153 pKa = 3.48RR154 pKa = 11.84PTRR157 pKa = 11.84VRR159 pKa = 11.84VAPALLGRR167 pKa = 11.84MALGLLGRR175 pKa = 11.84AVRR178 pKa = 11.84LGLLRR183 pKa = 11.84ARR185 pKa = 11.84LRR187 pKa = 11.84RR188 pKa = 11.84ARR190 pKa = 11.84GTSRR194 pKa = 11.84CPGRR198 pKa = 11.84AGPTVRR204 pKa = 11.84AVSTLPRR211 pKa = 11.84RR212 pKa = 11.84RR213 pKa = 11.84ATSARR218 pKa = 11.84TRR220 pKa = 11.84ATTPPPPVGDD230 pKa = 3.53RR231 pKa = 11.84LRR233 pKa = 11.84ALVGAAPRR241 pKa = 11.84RR242 pKa = 11.84TPAGDD247 pKa = 3.47RR248 pKa = 11.84QQGRR252 pKa = 11.84ATTTRR257 pKa = 11.84PRR259 pKa = 11.84APAGVRR265 pKa = 11.84PRR267 pKa = 11.84ARR269 pKa = 11.84AATARR274 pKa = 11.84RR275 pKa = 11.84RR276 pKa = 11.84APVPVRR282 pKa = 11.84QAVRR286 pKa = 11.84VTAALGRR293 pKa = 11.84VPAGDD298 pKa = 3.97LLRR301 pKa = 11.84GVLRR305 pKa = 11.84RR306 pKa = 11.84MPAGVLPVALRR317 pKa = 11.84VVLRR321 pKa = 11.84RR322 pKa = 11.84ARR324 pKa = 11.84VGVPRR329 pKa = 11.84TVPRR333 pKa = 11.84RR334 pKa = 11.84VLGGVRR340 pKa = 11.84GPVVPGRR347 pKa = 11.84TRR349 pKa = 11.84AGGLLTVPVAGVPRR363 pKa = 11.84RR364 pKa = 11.84APGGVLPRR372 pKa = 11.84VRR374 pKa = 11.84ALGLVPPATGRR385 pKa = 11.84AARR388 pKa = 11.84PGRR391 pKa = 11.84SRR393 pKa = 11.84RR394 pKa = 11.84RR395 pKa = 11.84LRR397 pKa = 11.84APGPPAPGTKK407 pKa = 10.25DD408 pKa = 3.34PAKK411 pKa = 10.79DD412 pKa = 3.09PATRR416 pKa = 11.84AQAVRR421 pKa = 11.84APRR424 pKa = 11.84TKK426 pKa = 10.82APVTRR431 pKa = 11.84GPVTRR436 pKa = 11.84GRR438 pKa = 11.84RR439 pKa = 11.84VGRR442 pKa = 11.84ATRR445 pKa = 11.84GRR447 pKa = 11.84GPGPPGFRR455 pKa = 11.84LTSSRR460 pKa = 11.84VVGRR464 pKa = 11.84APASSLPGSSRR475 pKa = 11.84PGSTRR480 pKa = 11.84RR481 pKa = 11.84SRR483 pKa = 11.84FRR485 pKa = 11.84SRR487 pKa = 11.84RR488 pKa = 11.84VVAAAASSGRR498 pKa = 11.84RR499 pKa = 11.84RR500 pKa = 11.84RR501 pKa = 11.84ACC503 pKa = 3.64

Molecular weight: 53.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7393

0

7393

2439600

27

6241

330.0

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.85 ± 0.033

0.679 ± 0.008

6.132 ± 0.027

5.463 ± 0.032

2.895 ± 0.017

9.079 ± 0.027

2.032 ± 0.013

3.656 ± 0.019

2.299 ± 0.022

10.529 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.625 ± 0.009

2.025 ± 0.018

5.822 ± 0.024

3.086 ± 0.017

7.464 ± 0.033

5.23 ± 0.022

6.397 ± 0.028

8.854 ± 0.026

1.641 ± 0.012

2.241 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski