Actinomadura chibensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D0NF79|A0A5D0NF79_9ACTN Uncharacterized protein OS=Actinomadura chibensis OX=392828 GN=FXF69_29380 PE=4 SV=1
MM1 pKa = 7.24TSYY4 pKa = 10.71IVVFGLIVVVVLVVVLVALGLRR26 pKa = 11.84ASKK29 pKa = 10.57AGRR32 pKa = 11.84DD33 pKa = 3.64DD34 pKa = 5.11DD35 pKa = 5.55DD36 pKa = 4.22WMDD39 pKa = 5.38DD40 pKa = 3.53EE41 pKa = 5.13PKK43 pKa = 10.28QPRR46 pKa = 11.84GRR48 pKa = 11.84RR49 pKa = 11.84GVPPPEE55 pKa = 5.04DD56 pKa = 2.88MGGPDD61 pKa = 3.43EE62 pKa = 4.28YY63 pKa = 11.29GGYY66 pKa = 10.44GDD68 pKa = 5.91GYY70 pKa = 10.22DD71 pKa = 3.17TGYY74 pKa = 11.01DD75 pKa = 3.01AGYY78 pKa = 9.79DD79 pKa = 3.19RR80 pKa = 11.84HH81 pKa = 6.16GAPEE85 pKa = 3.94PAAAPDD91 pKa = 3.45RR92 pKa = 11.84RR93 pKa = 11.84VAGGPLAAPTSAPAPPRR110 pKa = 11.84PSAPASDD117 pKa = 3.69EE118 pKa = 4.29MEE120 pKa = 5.6DD121 pKa = 3.45DD122 pKa = 5.39DD123 pKa = 4.41YY124 pKa = 11.55WATITFDD131 pKa = 3.31KK132 pKa = 11.02PKK134 pKa = 10.46FPWQHH139 pKa = 6.58DD140 pKa = 4.15EE141 pKa = 3.84NGEE144 pKa = 4.11RR145 pKa = 11.84PDD147 pKa = 3.59VDD149 pKa = 3.73PAADD153 pKa = 4.08PLNAQAPADD162 pKa = 4.17QPPVDD167 pKa = 4.32QPGPPHH173 pKa = 7.26PSLTQPALTQPVSMNADD190 pKa = 3.65GQPLGLDD197 pKa = 3.7GQPLGMGGGTGPQQVPAPGGFQGASPFAGHH227 pKa = 7.39DD228 pKa = 3.75PGSTALDD235 pKa = 4.99PIPTDD240 pKa = 3.68LGGQGMPGGQPSYY253 pKa = 10.57GGPGVPDD260 pKa = 3.24QQVYY264 pKa = 10.27GGQEE268 pKa = 3.62PQPAYY273 pKa = 10.38GAADD277 pKa = 3.59YY278 pKa = 11.48GGGEE282 pKa = 4.16PSYY285 pKa = 11.42GGGGQDD291 pKa = 4.16QGYY294 pKa = 10.03SDD296 pKa = 3.61QPQYY300 pKa = 11.33GSHH303 pKa = 6.49EE304 pKa = 4.19PPSYY308 pKa = 11.23GDD310 pKa = 3.67YY311 pKa = 11.18SSDD314 pKa = 3.56PLGGRR319 pKa = 11.84PPASPPSGGRR329 pKa = 11.84PDD331 pKa = 4.18AFGPSTTGPSTTGPSTTGPSTTGPTPAGPGGPLGGDD367 pKa = 3.4PRR369 pKa = 11.84VSDD372 pKa = 4.28PLGLPLGGRR381 pKa = 11.84PDD383 pKa = 3.88EE384 pKa = 4.58PAVPPPAPPAPPAPAAPAAHH404 pKa = 6.55TGGGSDD410 pKa = 3.51TDD412 pKa = 3.59GHH414 pKa = 6.47KK415 pKa = 10.89LPTVDD420 pKa = 5.42EE421 pKa = 4.2LLQRR425 pKa = 11.84IQNDD429 pKa = 3.79RR430 pKa = 11.84QRR432 pKa = 11.84SSGPSGSDD440 pKa = 2.78QGGSYY445 pKa = 10.88GGGSLNDD452 pKa = 4.02PLGDD456 pKa = 3.77PLGTGSFGGSSGSAAPGGGGNTGPWPSPGQSSGYY490 pKa = 10.38DD491 pKa = 3.12SGGGSGLPSGSGYY504 pKa = 10.8DD505 pKa = 3.47SGLGAPSGYY514 pKa = 10.72GQGQQNDD521 pKa = 5.03GYY523 pKa = 8.33PTAPAYY529 pKa = 10.51GEE531 pKa = 4.35TASPRR536 pKa = 11.84YY537 pKa = 9.51DD538 pKa = 3.69DD539 pKa = 4.56PLSGGRR545 pKa = 11.84EE546 pKa = 4.37SFGGTYY552 pKa = 10.48GGGAEE557 pKa = 4.35GGSNGGSSGSGGSGGVYY574 pKa = 10.6GDD576 pKa = 4.58FSGSSYY582 pKa = 11.54NGGNDD587 pKa = 3.92PLSAPPDD594 pKa = 3.94PNATQAYY601 pKa = 8.12GQGGYY606 pKa = 9.98SGGYY610 pKa = 7.06QQGGYY615 pKa = 7.39QQGQQNDD622 pKa = 3.62GSQYY626 pKa = 10.77GSRR629 pKa = 11.84PPADD633 pKa = 2.97DD634 pKa = 3.26WEE636 pKa = 4.34NYY638 pKa = 8.27RR639 pKa = 11.84RR640 pKa = 3.78

Molecular weight: 63.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D0NYS9|A0A5D0NYS9_9ACTN DUF3427 domain-containing protein OS=Actinomadura chibensis OX=392828 GN=FXF69_11965 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.24GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8099

0

8099

2744976

27

6628

338.9

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.08 ± 0.039

0.786 ± 0.008

6.21 ± 0.022

5.525 ± 0.026

2.776 ± 0.014

9.755 ± 0.026

2.156 ± 0.012

3.161 ± 0.017

1.923 ± 0.021

10.292 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.761 ± 0.01

1.672 ± 0.012

6.361 ± 0.026

2.337 ± 0.014

8.829 ± 0.026

4.68 ± 0.017

5.515 ± 0.023

8.701 ± 0.028

1.488 ± 0.009

1.992 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski