Halorhabdus tiamatea SARL4B

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halorhabdus; Halorhabdus tiamatea

Average proteome isoelectric point is 4.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2988 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7PJY1|F7PJY1_9EURY Uncharacterized protein OS=Halorhabdus tiamatea SARL4B OX=1033806 GN=HLRTI_000228 PE=4 SV=1
MM1 pKa = 7.19TAGDD5 pKa = 4.0DD6 pKa = 3.53THH8 pKa = 6.28TLNRR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.16VLKK17 pKa = 10.86SIGAGAVGTTSVLSARR33 pKa = 11.84SVIADD38 pKa = 3.32SDD40 pKa = 3.75GTHH43 pKa = 6.31YY44 pKa = 11.26SNPLYY49 pKa = 10.87GPDD52 pKa = 3.56FADD55 pKa = 3.7PTIHH59 pKa = 7.44RR60 pKa = 11.84ADD62 pKa = 4.54DD63 pKa = 4.08GTWWAYY69 pKa = 9.48ASNMSYY75 pKa = 10.41IDD77 pKa = 4.11DD78 pKa = 4.2ADD80 pKa = 4.01EE81 pKa = 4.12EE82 pKa = 4.55LVPILSSPDD91 pKa = 3.43LVNWTYY97 pKa = 11.0EE98 pKa = 3.92GEE100 pKa = 4.36AFDD103 pKa = 6.02SRR105 pKa = 11.84PGWLYY110 pKa = 11.14GSIWAPDD117 pKa = 3.32VHH119 pKa = 7.3YY120 pKa = 10.98HH121 pKa = 6.79DD122 pKa = 4.99GQWVLFYY129 pKa = 11.1ALWPRR134 pKa = 11.84GDD136 pKa = 5.28DD137 pKa = 3.63DD138 pKa = 4.37SQVPGIGVATADD150 pKa = 3.91TPDD153 pKa = 4.16GPFTDD158 pKa = 3.71HH159 pKa = 7.54GEE161 pKa = 4.06ILSNPDD167 pKa = 2.78HH168 pKa = 7.49PYY170 pKa = 10.15PGNTIDD176 pKa = 4.34PYY178 pKa = 11.19FVFHH182 pKa = 7.16NEE184 pKa = 3.48TPYY187 pKa = 11.26LFWANFAGINVVEE200 pKa = 4.37LTDD203 pKa = 3.99DD204 pKa = 3.61LRR206 pKa = 11.84DD207 pKa = 3.71YY208 pKa = 11.09RR209 pKa = 11.84AGTFDD214 pKa = 3.75QIAGSAYY221 pKa = 9.49EE222 pKa = 4.37GPAIFKK228 pKa = 10.68RR229 pKa = 11.84GDD231 pKa = 2.96YY232 pKa = 9.67WYY234 pKa = 10.91VFGSTGDD241 pKa = 3.62CCDD244 pKa = 4.4GFDD247 pKa = 3.29STYY250 pKa = 9.61EE251 pKa = 4.04VEE253 pKa = 4.46VGRR256 pKa = 11.84SEE258 pKa = 4.74YY259 pKa = 10.75FLGPYY264 pKa = 9.23HH265 pKa = 7.15DD266 pKa = 5.05RR267 pKa = 11.84NGTPMLEE274 pKa = 3.76RR275 pKa = 11.84DD276 pKa = 3.57EE277 pKa = 4.74WNAGPTHH284 pKa = 6.96LGDD287 pKa = 3.34NDD289 pKa = 3.74RR290 pKa = 11.84FVGPGHH296 pKa = 7.07GDD298 pKa = 3.24VTVDD302 pKa = 4.74DD303 pKa = 5.63DD304 pKa = 3.69GTYY307 pKa = 9.13WFVYY311 pKa = 9.18HH312 pKa = 7.5AYY314 pKa = 8.92DD315 pKa = 3.46TEE317 pKa = 4.96GPAFVDD323 pKa = 3.95SGWPPARR330 pKa = 11.84QLFVDD335 pKa = 4.87PIRR338 pKa = 11.84WEE340 pKa = 4.44DD341 pKa = 3.08GWPIIGCDD349 pKa = 3.6GTPSIEE355 pKa = 4.04MPIPGEE361 pKa = 4.0GGYY364 pKa = 10.81CSGSDD369 pKa = 4.44DD370 pKa = 3.74GTGDD374 pKa = 3.37GGGDD378 pKa = 3.61GTDD381 pKa = 3.76DD382 pKa = 5.26RR383 pKa = 11.84YY384 pKa = 9.71EE385 pKa = 4.15TWSDD389 pKa = 2.96TDD391 pKa = 3.51DD392 pKa = 4.27PYY394 pKa = 11.93YY395 pKa = 10.11STLVSDD401 pKa = 4.23LEE403 pKa = 4.59GYY405 pKa = 10.15NLASAGQFVNGNEE418 pKa = 4.16EE419 pKa = 3.85ATVWDD424 pKa = 4.21TYY426 pKa = 11.24EE427 pKa = 4.34IDD429 pKa = 3.73GGNADD434 pKa = 3.89RR435 pKa = 11.84LEE437 pKa = 4.28RR438 pKa = 11.84SSLAVSDD445 pKa = 4.14EE446 pKa = 4.52VPFSEE451 pKa = 4.23AARR454 pKa = 11.84FEE456 pKa = 4.5VTDD459 pKa = 3.63TPEE462 pKa = 4.24NPWEE466 pKa = 4.03ITLKK470 pKa = 10.79SVGEE474 pKa = 4.09RR475 pKa = 11.84EE476 pKa = 4.3LEE478 pKa = 4.21SGHH481 pKa = 6.12VLLGVAYY488 pKa = 9.27MRR490 pKa = 11.84TPDD493 pKa = 3.42EE494 pKa = 4.38EE495 pKa = 5.31ASVTYY500 pKa = 10.55KK501 pKa = 9.81STASSNEE508 pKa = 3.2SDD510 pKa = 3.6NYY512 pKa = 7.88VTRR515 pKa = 11.84AQPPLDD521 pKa = 4.17TEE523 pKa = 3.81WQRR526 pKa = 11.84YY527 pKa = 5.32YY528 pKa = 11.03FPIEE532 pKa = 4.32FGVSAAPGEE541 pKa = 4.22WWTEE545 pKa = 3.29IWLGAAAQTVDD556 pKa = 2.83IGGLAVIDD564 pKa = 4.11FARR567 pKa = 11.84GVRR570 pKa = 11.84ADD572 pKa = 4.26DD573 pKa = 4.22LPVGEE578 pKa = 5.46DD579 pKa = 3.24PGEE582 pKa = 4.25TAVEE586 pKa = 4.1TDD588 pKa = 4.28DD589 pKa = 3.66GTEE592 pKa = 3.97TVTSGEE598 pKa = 4.42SEE600 pKa = 4.31GEE602 pKa = 3.8QDD604 pKa = 5.46DD605 pKa = 5.76DD606 pKa = 3.92EE607 pKa = 4.98TSDD610 pKa = 4.88SEE612 pKa = 4.42GTPADD617 pKa = 4.11ALPGGEE623 pKa = 5.05GSSQDD628 pKa = 3.42LDD630 pKa = 3.78GDD632 pKa = 4.07GLYY635 pKa = 10.83EE636 pKa = 5.18DD637 pKa = 4.61VDD639 pKa = 4.15GDD641 pKa = 3.75GDD643 pKa = 4.34ADD645 pKa = 3.49ISDD648 pKa = 3.75VRR650 pKa = 11.84SLLTNFNSEE659 pKa = 4.33MVQEE663 pKa = 4.57HH664 pKa = 6.85ADD666 pKa = 3.51AYY668 pKa = 11.04DD669 pKa = 3.35FDD671 pKa = 6.51GDD673 pKa = 3.93GGVDD677 pKa = 3.4VGDD680 pKa = 3.54VLALYY685 pKa = 9.77RR686 pKa = 11.84RR687 pKa = 11.84SYY689 pKa = 11.13RR690 pKa = 11.84SVTT693 pKa = 3.29

Molecular weight: 75.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7PNT0|F7PNT0_9EURY Pyruvate synthase subunit PorA protein OS=Halorhabdus tiamatea SARL4B OX=1033806 GN=porA PE=4 SV=1
MM1 pKa = 7.13ATSSEE6 pKa = 3.88KK7 pKa = 10.42RR8 pKa = 11.84EE9 pKa = 3.73RR10 pKa = 11.84AMAAMVKK17 pKa = 9.99HH18 pKa = 5.38YY19 pKa = 10.57GEE21 pKa = 5.0RR22 pKa = 11.84RR23 pKa = 11.84QQLNPFDD30 pKa = 4.38EE31 pKa = 4.43GRR33 pKa = 11.84KK34 pKa = 8.47YY35 pKa = 10.54HH36 pKa = 7.35DD37 pKa = 4.13SFAVGYY43 pKa = 9.52SGGYY47 pKa = 8.65AASFLVPVAAEE58 pKa = 3.94AKK60 pKa = 9.63AAQAAARR67 pKa = 11.84MPYY70 pKa = 8.85MAALLRR76 pKa = 11.84SVNTMKK82 pKa = 10.7RR83 pKa = 11.84SIKK86 pKa = 10.45GVTIGSAMWTGGKK99 pKa = 8.87IARR102 pKa = 11.84RR103 pKa = 11.84MPDD106 pKa = 2.24VDD108 pKa = 4.22FNVGRR113 pKa = 11.84RR114 pKa = 11.84VSDD117 pKa = 3.54VEE119 pKa = 3.67LDD121 pKa = 3.47GRR123 pKa = 11.84AKK125 pKa = 10.63QIVGQTADD133 pKa = 3.57TLRR136 pKa = 11.84NTPLPRR142 pKa = 11.84QRR144 pKa = 11.84RR145 pKa = 11.84IAKK148 pKa = 7.69LTRR151 pKa = 11.84NTGDD155 pKa = 3.4GDD157 pKa = 3.73VMEE160 pKa = 4.43WLTRR164 pKa = 11.84NVEE167 pKa = 4.15APDD170 pKa = 3.32SEE172 pKa = 4.77RR173 pKa = 11.84YY174 pKa = 8.36QRR176 pKa = 11.84GLVYY180 pKa = 10.7LRR182 pKa = 11.84RR183 pKa = 11.84TGAEE187 pKa = 4.14GKK189 pKa = 9.74QLLSRR194 pKa = 11.84LTQDD198 pKa = 3.94ARR200 pKa = 11.84SALLKK205 pKa = 10.9LRR207 pKa = 11.84DD208 pKa = 3.49KK209 pKa = 11.43AGLQRR214 pKa = 11.84QLTKK218 pKa = 10.47AWVRR222 pKa = 11.84GDD224 pKa = 3.42IDD226 pKa = 4.04ASDD229 pKa = 3.16IGKK232 pKa = 8.64ATRR235 pKa = 11.84RR236 pKa = 11.84YY237 pKa = 10.42DD238 pKa = 3.46GLDD241 pKa = 3.21AEE243 pKa = 4.99GRR245 pKa = 11.84AAFDD249 pKa = 4.92DD250 pKa = 4.41LVQHH254 pKa = 6.77EE255 pKa = 5.12GPDD258 pKa = 3.67AVSVSGKK265 pKa = 9.92LDD267 pKa = 3.23EE268 pKa = 5.71DD269 pKa = 3.7AFEE272 pKa = 5.46DD273 pKa = 3.96VLKK276 pKa = 10.98SDD278 pKa = 4.58LSTARR283 pKa = 11.84KK284 pKa = 9.71ADD286 pKa = 3.39IVDD289 pKa = 3.76SLSEE293 pKa = 3.97VDD295 pKa = 4.61QMQSFNTSTIEE306 pKa = 4.01LVSRR310 pKa = 11.84PSGWRR315 pKa = 11.84GTCAGIVWRR324 pKa = 11.84RR325 pKa = 11.84SFAGPTPTRR334 pKa = 11.84RR335 pKa = 11.84TSWPTGRR342 pKa = 11.84ANWMCSSTVPSPMRR356 pKa = 11.84SRR358 pKa = 11.84TVTLAVMSSRR368 pKa = 11.84RR369 pKa = 11.84RR370 pKa = 11.84STRR373 pKa = 11.84SMSWRR378 pKa = 11.84TSGGRR383 pKa = 11.84VRR385 pKa = 11.84ANSASVSSSNHH396 pKa = 4.16PTAAVSSSPPSTTPRR411 pKa = 11.84FTNSVPKK418 pKa = 10.66

Molecular weight: 45.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2988

0

2988

856988

37

2728

286.8

31.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.501 ± 0.062

0.639 ± 0.017

8.697 ± 0.047

8.956 ± 0.063

3.269 ± 0.027

8.201 ± 0.05

2.049 ± 0.025

4.393 ± 0.031

1.857 ± 0.03

8.802 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.737 ± 0.02

2.399 ± 0.032

4.592 ± 0.029

2.729 ± 0.027

6.467 ± 0.047

5.493 ± 0.033

6.566 ± 0.043

8.709 ± 0.047

1.186 ± 0.02

2.759 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski