Rhizobium phage vB_RleM_PPF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068CDG1|A0A068CDG1_9CAUD Uncharacterized protein OS=Rhizobium phage vB_RleM_PPF1 OX=1498228 GN=PPF1_52 PE=4 SV=1
MM1 pKa = 8.04SYY3 pKa = 10.32QCDD6 pKa = 3.73DD7 pKa = 3.88ADD9 pKa = 5.54DD10 pKa = 4.82YY11 pKa = 12.05DD12 pKa = 5.54DD13 pKa = 5.53EE14 pKa = 4.97PSCICPHH21 pKa = 6.82CDD23 pKa = 2.8GFGSRR28 pKa = 11.84EE29 pKa = 4.3CYY31 pKa = 10.25CCGDD35 pKa = 4.23FCCCDD40 pKa = 2.94NGGEE44 pKa = 4.37IEE46 pKa = 4.27CSYY49 pKa = 11.19CRR51 pKa = 11.84GEE53 pKa = 4.4SEE55 pKa = 4.5VSQTRR60 pKa = 11.84YY61 pKa = 9.51EE62 pKa = 4.24YY63 pKa = 10.14YY64 pKa = 10.48LRR66 pKa = 11.84RR67 pKa = 11.84QTEE70 pKa = 4.01HH71 pKa = 6.2AKK73 pKa = 10.27EE74 pKa = 3.96RR75 pKa = 11.84AEE77 pKa = 4.0VWANLAAEE85 pKa = 4.64RR86 pKa = 11.84EE87 pKa = 4.43AATPAPIEE95 pKa = 4.06EE96 pKa = 4.5TKK98 pKa = 10.66PP99 pKa = 3.33

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068CHB5|A0A068CHB5_9CAUD Uncharacterized protein OS=Rhizobium phage vB_RleM_PPF1 OX=1498228 GN=PPF1_89 PE=4 SV=1
MM1 pKa = 7.18QPEE4 pKa = 3.94IDD6 pKa = 3.58RR7 pKa = 11.84ARR9 pKa = 11.84LSRR12 pKa = 11.84DD13 pKa = 2.72VRR15 pKa = 11.84RR16 pKa = 11.84WLSDD20 pKa = 3.14NQLTTRR26 pKa = 11.84SAPLAHH32 pKa = 7.04RR33 pKa = 11.84GLNPAMISRR42 pKa = 11.84ACSEE46 pKa = 4.91SILSAASMLALCAAMKK62 pKa = 10.14RR63 pKa = 11.84DD64 pKa = 3.47PTTYY68 pKa = 10.78LIFLDD73 pKa = 4.14KK74 pKa = 10.79RR75 pKa = 11.84NQAVTANVHH84 pKa = 5.77RR85 pKa = 11.84EE86 pKa = 3.78TRR88 pKa = 11.84GAQQ91 pKa = 2.99

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

17406

37

711

185.2

20.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.025 ± 0.362

1.178 ± 0.132

6.429 ± 0.188

5.803 ± 0.278

3.206 ± 0.156

8.055 ± 0.234

2.028 ± 0.185

5.32 ± 0.178

4.102 ± 0.227

8.106 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.121

3.079 ± 0.153

5.159 ± 0.163

3.108 ± 0.151

7.314 ± 0.23

5.94 ± 0.239

5.808 ± 0.27

6.567 ± 0.224

1.706 ± 0.101

2.378 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski