Scylla serrata reovirus SZ-2007
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9BDA7|G9BDA7_9REOV VP11 OS=Scylla serrata reovirus SZ-2007 OX=458682 GN=MCRV_s11gp1 PE=4 SV=1
MM1 pKa = 7.86 ADD3 pKa = 3.84 SITGRR8 pKa = 11.84 SGAGLPMSHH17 pKa = 6.78 TYY19 pKa = 10.58 GYY21 pKa = 10.73 QILLQKK27 pKa = 9.53 QQYY30 pKa = 10.23 DD31 pKa = 3.19 HH32 pKa = 7.31 DD33 pKa = 4.21 TTVPSEE39 pKa = 4.45 STWTPFEE46 pKa = 5.29 SNDD49 pKa = 3.22 QYY51 pKa = 11.52 TIARR55 pKa = 11.84 DD56 pKa = 3.47 TEE58 pKa = 4.23 AYY60 pKa = 10.98 LNFFQIGTRR69 pKa = 11.84 VFFTVTPGPYY79 pKa = 10.26 GDD81 pKa = 4.15 LQEE84 pKa = 4.26 QFRR87 pKa = 11.84 SLRR90 pKa = 11.84 LKK92 pKa = 10.18 VIWTKK97 pKa = 11.11 NGGLAYY103 pKa = 10.3 SEE105 pKa = 4.28 EE106 pKa = 4.42 RR107 pKa = 11.84 TSILHH112 pKa = 5.71 WLQRR116 pKa = 11.84 LANTKK121 pKa = 10.42 SILTQCPVFLVCDD134 pKa = 3.69 NFEE137 pKa = 4.09 RR138 pKa = 11.84 LYY140 pKa = 11.46 VLLPCTMNKK149 pKa = 10.17 DD150 pKa = 3.85 YY151 pKa = 10.89 PCGSFAVRR159 pKa = 11.84 KK160 pKa = 9.65 KK161 pKa = 10.28 IPKK164 pKa = 9.54 QYY166 pKa = 11.18 AVLARR171 pKa = 11.84 LMNLVPSSKK180 pKa = 10.32 QEE182 pKa = 3.87 NNLYY186 pKa = 9.65 VYY188 pKa = 11.41 NMLKK192 pKa = 10.5 ALRR195 pKa = 11.84 LRR197 pKa = 11.84 SDD199 pKa = 4.1 DD200 pKa = 3.69 STEE203 pKa = 5.45 LIPTDD208 pKa = 3.37 DD209 pKa = 4.43 AARR212 pKa = 11.84 VMAPYY217 pKa = 9.08 LTDD220 pKa = 3.77 EE221 pKa = 4.75 DD222 pKa = 4.04 TSSIIRR228 pKa = 11.84 GALLGLEE235 pKa = 4.48 PDD237 pKa = 4.01 SIVIRR242 pKa = 11.84 WCNLLTDD249 pKa = 4.09 YY250 pKa = 11.02 DD251 pKa = 4.06 IEE253 pKa = 4.41 LTTSQWYY260 pKa = 10.12 FDD262 pKa = 3.4 TKK264 pKa = 11.06 VIVEE268 pKa = 4.65 KK269 pKa = 9.72 MWKK272 pKa = 7.33 TQSTPKK278 pKa = 9.84 IDD280 pKa = 3.44 ASTFLPEE287 pKa = 3.86 YY288 pKa = 11.36 DD289 pKa = 3.8 MLNLLSIKK297 pKa = 9.94 KK298 pKa = 10.23 GKK300 pKa = 9.87 ALYY303 pKa = 9.41 EE304 pKa = 4.1 DD305 pKa = 3.92 WVASAKK311 pKa = 10.63 EE312 pKa = 4.09 SDD314 pKa = 3.61 HH315 pKa = 6.91 NGALVRR321 pKa = 11.84 AGKK324 pKa = 10.51 YY325 pKa = 10.38 GIEE328 pKa = 4.19 NGDD331 pKa = 3.28 LTGRR335 pKa = 11.84 EE336 pKa = 3.81 TFIPVWLEE344 pKa = 3.6 QLITKK349 pKa = 10.28 ADD351 pKa = 3.24 VYY353 pKa = 10.9 YY354 pKa = 11.25
Molecular weight: 40.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.169
IPC2_protein 5.334
IPC_protein 5.27
Toseland 5.245
ProMoST 5.436
Dawson 5.296
Bjellqvist 5.41
Wikipedia 5.194
Rodwell 5.207
Grimsley 5.181
Solomon 5.296
Lehninger 5.258
Nozaki 5.436
DTASelect 5.614
Thurlkill 5.283
EMBOSS 5.27
Sillero 5.499
Patrickios 3.821
IPC_peptide 5.308
IPC2_peptide 5.499
IPC2.peptide.svr19 5.503
Protein with the highest isoelectric point:
>tr|H9NEU8|H9NEU8_9REOV Uncharacterized protein OS=Scylla serrata reovirus SZ-2007 OX=458682 PE=4 SV=1
MM1 pKa = 7.19 NWSKK5 pKa = 11.16 AINFQPFMLEE15 pKa = 3.66 TRR17 pKa = 11.84 PPLTTIPIMDD27 pKa = 3.77 QLVEE31 pKa = 3.76 IGEE34 pKa = 4.33 RR35 pKa = 11.84 SNQKK39 pKa = 9.57 WSMTDD44 pKa = 2.76 RR45 pKa = 11.84 LFFAIRR51 pKa = 11.84 KK52 pKa = 8.0 INPIFVTSSQIPSKK66 pKa = 10.63 FDD68 pKa = 3.24 YY69 pKa = 10.54 TILQMPTQLIASLKK83 pKa = 8.12 EE84 pKa = 3.91 TLLFLAFSYY93 pKa = 10.12 YY94 pKa = 10.27 LRR96 pKa = 11.84 EE97 pKa = 4.14 YY98 pKa = 9.45 QDD100 pKa = 4.12 KK101 pKa = 9.27 VGQMKK106 pKa = 9.36 FYY108 pKa = 9.98 PVAMKK113 pKa = 11.03 NMIPIVNYY121 pKa = 10.72 LKK123 pKa = 10.97 DD124 pKa = 3.5 RR125 pKa = 11.84 VHH127 pKa = 6.76 NNFDD131 pKa = 3.51 TTLEE135 pKa = 3.7 QAYY138 pKa = 9.12 RR139 pKa = 11.84 QNVVHH144 pKa = 6.41 TLSASDD150 pKa = 4.88 AFDD153 pKa = 4.49 LLSGMIATTRR163 pKa = 11.84 LDD165 pKa = 4.49 LIQRR169 pKa = 11.84 TRR171 pKa = 11.84 ICPEE175 pKa = 3.85 LLNVLNKK182 pKa = 9.85 MSFILIYY189 pKa = 10.61 APNRR193 pKa = 11.84 PSILSWKK200 pKa = 8.75 NQSS203 pKa = 2.99
Molecular weight: 23.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.837
IPC2_protein 8.916
IPC_protein 8.902
Toseland 9.428
ProMoST 9.253
Dawson 9.736
Bjellqvist 9.458
Wikipedia 9.926
Rodwell 9.94
Grimsley 9.823
Solomon 9.794
Lehninger 9.736
Nozaki 9.458
DTASelect 9.443
Thurlkill 9.545
EMBOSS 9.867
Sillero 9.648
Patrickios 4.863
IPC_peptide 9.78
IPC2_peptide 8.068
IPC2.peptide.svr19 7.824
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
6608
203
1422
550.7
62.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.568 ± 0.615
0.878 ± 0.199
6.326 ± 0.259
5.16 ± 0.183
3.435 ± 0.189
5.145 ± 0.409
2.467 ± 0.298
7.234 ± 0.386
5.176 ± 0.279
9.11 ± 0.331
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.906 ± 0.221
4.54 ± 0.206
4.222 ± 0.395
4.54 ± 0.098
6.129 ± 0.393
7.491 ± 0.262
6.674 ± 0.479
7.052 ± 0.368
0.938 ± 0.138
4.01 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here