Kudzu mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5H149|A5H149_9GEMI AC4 protein OS=Kudzu mosaic virus OX=390437 GN=AC4 PE=3 SV=1
MM1 pKa = 7.39SRR3 pKa = 11.84PKK5 pKa = 10.63GFRR8 pKa = 11.84VNAKK12 pKa = 10.02NFFLTYY18 pKa = 9.42PRR20 pKa = 11.84CSLSKK25 pKa = 10.39EE26 pKa = 3.81AALEE30 pKa = 3.79QLQNIQTNVNKK41 pKa = 10.41KK42 pKa = 9.79FIRR45 pKa = 11.84VCRR48 pKa = 11.84EE49 pKa = 3.17FHH51 pKa = 7.08EE52 pKa = 4.54NGEE55 pKa = 4.19PHH57 pKa = 6.75LHH59 pKa = 6.18VLLQFEE65 pKa = 5.55GKK67 pKa = 8.94FQCRR71 pKa = 11.84NEE73 pKa = 3.98RR74 pKa = 11.84FFDD77 pKa = 3.88LVSEE81 pKa = 4.27TRR83 pKa = 11.84SAHH86 pKa = 4.92FHH88 pKa = 6.58PNIQGAKK95 pKa = 9.58SSSDD99 pKa = 3.05VKK101 pKa = 11.03KK102 pKa = 11.15YY103 pKa = 8.81MEE105 pKa = 5.11KK106 pKa = 10.77DD107 pKa = 2.85GDD109 pKa = 4.05VIDD112 pKa = 5.28FGIFQIDD119 pKa = 3.36GRR121 pKa = 11.84SNRR124 pKa = 11.84GGSQCANDD132 pKa = 4.53AYY134 pKa = 10.9AEE136 pKa = 4.79AINSGDD142 pKa = 3.38TTSALNILKK151 pKa = 10.08EE152 pKa = 3.89KK153 pKa = 10.38ASRR156 pKa = 11.84DD157 pKa = 4.27FIIHH161 pKa = 6.14LHH163 pKa = 6.23NIRR166 pKa = 11.84ANLNFLFAPPPTVYY180 pKa = 7.57EE181 pKa = 4.36TPFSIEE187 pKa = 3.81SFNNVPEE194 pKa = 4.43TLTSWAAEE202 pKa = 3.84NVVCPAARR210 pKa = 11.84PFRR213 pKa = 11.84PISIVVEE220 pKa = 4.42GEE222 pKa = 3.93SRR224 pKa = 11.84TGKK227 pKa = 8.58TMWARR232 pKa = 11.84SLGRR236 pKa = 11.84HH237 pKa = 5.8NYY239 pKa = 10.07LCGHH243 pKa = 7.45LDD245 pKa = 3.94LSAKK249 pKa = 10.22VYY251 pKa = 11.33SNDD254 pKa = 2.23AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.76EE273 pKa = 4.49FMGAQKK279 pKa = 10.61DD280 pKa = 3.51WQSNVKK286 pKa = 9.81YY287 pKa = 10.5GKK289 pKa = 7.69PTQIKK294 pKa = 10.11GGIPTIFLCNAGPRR308 pKa = 11.84SSYY311 pKa = 11.61KK312 pKa = 9.97MFLDD316 pKa = 3.75EE317 pKa = 5.3EE318 pKa = 4.62NNASLKK324 pKa = 10.19EE325 pKa = 3.99WALKK329 pKa = 10.25NAIFYY334 pKa = 8.9TLTEE338 pKa = 4.36PLFSTTNQGATQVGQEE354 pKa = 4.44TCNSTQTNN362 pKa = 3.46

Molecular weight: 41.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5H150|A5H150_9GEMI Protein V2 OS=Kudzu mosaic virus OX=390437 GN=AV2 PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 9.84MGNLTSMFCFNLKK15 pKa = 9.94EE16 pKa = 4.03NSSAATKK23 pKa = 10.4GSSTSYY29 pKa = 9.88PKK31 pKa = 10.2PGQHH35 pKa = 5.64ISIRR39 pKa = 11.84TFRR42 pKa = 11.84EE43 pKa = 3.29LRR45 pKa = 11.84AAQMLKK51 pKa = 8.92STWRR55 pKa = 11.84KK56 pKa = 7.82TEE58 pKa = 3.72TSS60 pKa = 3.23

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1635

60

362

204.4

23.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.872 ± 0.415

2.263 ± 0.263

4.893 ± 0.435

4.343 ± 0.599

4.709 ± 0.573

5.505 ± 0.556

3.425 ± 0.559

4.893 ± 0.553

6.055 ± 0.256

8.196 ± 0.807

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.297

5.627 ± 0.646

5.749 ± 0.655

3.609 ± 0.321

6.972 ± 0.806

8.257 ± 0.541

6.055 ± 0.821

6.055 ± 0.592

1.284 ± 0.196

3.914 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski