Sphingopyxis sp. GW247-27LB

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; unclassified Sphingopyxis

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A270BBJ4|A0A270BBJ4_9SPHN Cell division protein OS=Sphingopyxis sp. GW247-27LB OX=2012632 GN=CD928_14285 PE=4 SV=1
MM1 pKa = 7.72PSVNFQSADD10 pKa = 3.36GAVTTIEE17 pKa = 3.99IEE19 pKa = 3.83IGNSVMRR26 pKa = 11.84GARR29 pKa = 11.84DD30 pKa = 3.53NSVDD34 pKa = 5.86GIDD37 pKa = 3.76ADD39 pKa = 4.0CGGVCACATCHH50 pKa = 6.7VYY52 pKa = 10.18VAPDD56 pKa = 3.47WIDD59 pKa = 3.19RR60 pKa = 11.84VGPAGTDD67 pKa = 3.18EE68 pKa = 6.02SEE70 pKa = 4.66MLDD73 pKa = 3.7CVNDD77 pKa = 3.57PRR79 pKa = 11.84PNSRR83 pKa = 11.84LSCQIAMSQEE93 pKa = 4.16LEE95 pKa = 4.13GLTLILPEE103 pKa = 4.24SQRR106 pKa = 3.48

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A270BBX4|A0A270BBX4_9SPHN Cell shape determination protein CcmA OS=Sphingopyxis sp. GW247-27LB OX=2012632 GN=CD928_09585 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.57GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.1VLANRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4437

0

4437

1429297

29

2758

322.1

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.855 ± 0.065

0.782 ± 0.013

6.205 ± 0.029

5.399 ± 0.031

3.562 ± 0.022

8.977 ± 0.041

1.964 ± 0.018

4.994 ± 0.024

2.971 ± 0.028

9.731 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.018

2.464 ± 0.028

5.373 ± 0.03

2.936 ± 0.02

7.463 ± 0.041

5.149 ± 0.03

5.099 ± 0.027

6.946 ± 0.028

1.509 ± 0.017

2.245 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski