Amino acid dipepetide frequency for Sphingopyxis sp. GW247-27LB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.602AlaAla: 21.602 ± 0.21	1.035AlaCys: 1.035 ± 0.034	8.368AlaAsp: 8.368 ± 0.091	8.015AlaGlu: 8.015 ± 0.093	4.453AlaPhe: 4.453 ± 0.048	11.963AlaGly: 11.963 ± 0.105	2.41AlaHis: 2.41 ± 0.047	7.05AlaIle: 7.05 ± 0.086	4.428AlaLys: 4.428 ± 0.068	14.096AlaLeu: 14.096 ± 0.131	3.992AlaMet: 3.992 ± 0.059	3.255AlaAsn: 3.255 ± 0.056	6.931AlaPro: 6.931 ± 0.101	4.147AlaGln: 4.147 ± 0.058	10.107AlaArg: 10.107 ± 0.118	6.91AlaSer: 6.91 ± 0.075	6.68AlaThr: 6.68 ± 0.074	8.638AlaVal: 8.638 ± 0.089	1.855AlaTrp: 1.855 ± 0.043	2.612AlaTyr: 2.612 ± 0.049	0.003AlaXaa: 0.003 ± 0.001
Cys	0.995CysAla: 0.995 ± 0.03	0.106CysCys: 0.106 ± 0.009	0.544CysAsp: 0.544 ± 0.019	0.423CysGlu: 0.423 ± 0.018	0.292CysPhe: 0.292 ± 0.013	0.832CysGly: 0.832 ± 0.023	0.199CysHis: 0.199 ± 0.016	0.335CysIle: 0.335 ± 0.015	0.162CysLys: 0.162 ± 0.011	0.675CysLeu: 0.675 ± 0.026	0.136CysMet: 0.136 ± 0.01	0.202CysAsn: 0.202 ± 0.012	0.452CysPro: 0.452 ± 0.021	0.219CysGln: 0.219 ± 0.013	0.607CysArg: 0.607 ± 0.022	0.449CysSer: 0.449 ± 0.019	0.375CysThr: 0.375 ± 0.016	0.516CysVal: 0.516 ± 0.019	0.115CysTrp: 0.115 ± 0.009	0.185CysTyr: 0.185 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.646AspAla: 8.646 ± 0.085	0.503AspCys: 0.503 ± 0.018	3.563AspAsp: 3.563 ± 0.064	3.556AspGlu: 3.556 ± 0.058	2.312AspPhe: 2.312 ± 0.048	5.875AspGly: 5.875 ± 0.069	1.349AspHis: 1.349 ± 0.031	3.073AspIle: 3.073 ± 0.046	1.737AspLys: 1.737 ± 0.041	5.874AspLeu: 5.874 ± 0.069	1.402AspMet: 1.402 ± 0.035	1.401AspAsn: 1.401 ± 0.033	3.961AspPro: 3.961 ± 0.06	1.701AspGln: 1.701 ± 0.038	5.121AspArg: 5.121 ± 0.065	2.424AspSer: 2.424 ± 0.041	2.57AspThr: 2.57 ± 0.047	4.006AspVal: 4.006 ± 0.059	1.198AspTrp: 1.198 ± 0.029	1.781AspTyr: 1.781 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.153GluAla: 8.153 ± 0.088	0.355GluCys: 0.355 ± 0.017	2.724GluAsp: 2.724 ± 0.049	2.837GluGlu: 2.837 ± 0.055	1.485GluPhe: 1.485 ± 0.034	4.688GluGly: 4.688 ± 0.067	1.028GluHis: 1.028 ± 0.027	2.967GluIle: 2.967 ± 0.051	1.897GluLys: 1.897 ± 0.044	5.076GluLeu: 5.076 ± 0.067	1.355GluMet: 1.355 ± 0.032	1.297GluAsn: 1.297 ± 0.033	2.636GluPro: 2.636 ± 0.047	1.885GluGln: 1.885 ± 0.035	4.944GluArg: 4.944 ± 0.072	2.236GluSer: 2.236 ± 0.037	3.057GluThr: 3.057 ± 0.048	3.586GluVal: 3.586 ± 0.059	0.812GluTrp: 0.812 ± 0.025	0.976GluTyr: 0.976 ± 0.024	0.001GluXaa: 0.001 ± 0.001
Phe	5.044PheAla: 5.044 ± 0.058	0.289PheCys: 0.289 ± 0.014	2.876PheAsp: 2.876 ± 0.045	2.06PheGlu: 2.06 ± 0.039	1.259PhePhe: 1.259 ± 0.034	3.777PheGly: 3.777 ± 0.06	0.687PheHis: 0.687 ± 0.024	1.416PheIle: 1.416 ± 0.034	0.848PheLys: 0.848 ± 0.028	3.107PheLeu: 3.107 ± 0.053	0.703PheMet: 0.703 ± 0.021	1.017PheAsn: 1.017 ± 0.033	1.583PhePro: 1.583 ± 0.032	0.898PheGln: 0.898 ± 0.024	2.267PheArg: 2.267 ± 0.04	1.899PheSer: 1.899 ± 0.045	1.87PheThr: 1.87 ± 0.04	2.613PheVal: 2.613 ± 0.048	0.535PheTrp: 0.535 ± 0.02	0.873PheTyr: 0.873 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.543GlyAla: 10.543 ± 0.1	0.809GlyCys: 0.809 ± 0.028	5.387GlyAsp: 5.387 ± 0.071	5.202GlyGlu: 5.202 ± 0.074	3.602GlyPhe: 3.602 ± 0.054	8.523GlyGly: 8.523 ± 0.122	1.87GlyHis: 1.87 ± 0.035	4.394GlyIle: 4.394 ± 0.06	3.253GlyLys: 3.253 ± 0.064	8.517GlyLeu: 8.517 ± 0.093	2.295GlyMet: 2.295 ± 0.047	2.235GlyAsn: 2.235 ± 0.052	3.68GlyPro: 3.68 ± 0.058	2.987GlyGln: 2.987 ± 0.047	6.558GlyArg: 6.558 ± 0.079	4.858GlySer: 4.858 ± 0.079	4.772GlyThr: 4.772 ± 0.078	6.16GlyVal: 6.16 ± 0.077	1.748GlyTrp: 1.748 ± 0.038	2.374GlyTyr: 2.374 ± 0.043	0.001GlyXaa: 0.001 ± 0.001
His	2.327HisAla: 2.327 ± 0.044	0.227HisCys: 0.227 ± 0.014	1.22HisAsp: 1.22 ± 0.03	0.954HisGlu: 0.954 ± 0.026	0.798HisPhe: 0.798 ± 0.021	1.956HisGly: 1.956 ± 0.04	0.577HisHis: 0.577 ± 0.023	0.954HisIle: 0.954 ± 0.026	0.449HisLys: 0.449 ± 0.015	1.839HisLeu: 1.839 ± 0.045	0.507HisMet: 0.507 ± 0.018	0.451HisAsn: 0.451 ± 0.018	1.301HisPro: 1.301 ± 0.032	0.536HisGln: 0.536 ± 0.018	1.56HisArg: 1.56 ± 0.034	0.935HisSer: 0.935 ± 0.023	0.607HisThr: 0.607 ± 0.023	1.461HisVal: 1.461 ± 0.031	0.376HisTrp: 0.376 ± 0.016	0.606HisTyr: 0.606 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.984IleAla: 7.984 ± 0.083	0.409IleCys: 0.409 ± 0.02	4.092IleAsp: 4.092 ± 0.058	3.395IleGlu: 3.395 ± 0.05	1.641IlePhe: 1.641 ± 0.041	5.001IleGly: 5.001 ± 0.063	0.859IleHis: 0.859 ± 0.026	2.004IleIle: 2.004 ± 0.041	1.159IleLys: 1.159 ± 0.032	4.148IleLeu: 4.148 ± 0.054	0.83IleMet: 0.83 ± 0.025	1.26IleAsn: 1.26 ± 0.032	2.199IlePro: 2.199 ± 0.038	1.084IleGln: 1.084 ± 0.03	3.243IleArg: 3.243 ± 0.046	2.484IleSer: 2.484 ± 0.043	2.343IleThr: 2.343 ± 0.047	4.158IleVal: 4.158 ± 0.063	0.635IleTrp: 0.635 ± 0.022	1.012IleTyr: 1.012 ± 0.031	0.001IleXaa: 0.001 ± 0.001
Lys	4.161LysAla: 4.161 ± 0.064	0.167LysCys: 0.167 ± 0.011	1.623LysAsp: 1.623 ± 0.039	1.308LysGlu: 1.308 ± 0.035	0.828LysPhe: 0.828 ± 0.026	2.692LysGly: 2.692 ± 0.05	0.531LysHis: 0.531 ± 0.017	1.429LysIle: 1.429 ± 0.037	1.07LysLys: 1.07 ± 0.036	3.033LysLeu: 3.033 ± 0.053	0.695LysMet: 0.695 ± 0.025	0.694LysAsn: 0.694 ± 0.023	1.944LysPro: 1.944 ± 0.042	0.879LysGln: 0.879 ± 0.026	2.331LysArg: 2.331 ± 0.047	1.605LysSer: 1.605 ± 0.034	1.648LysThr: 1.648 ± 0.037	2.049LysVal: 2.049 ± 0.038	0.432LysTrp: 0.432 ± 0.019	0.595LysTyr: 0.595 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	14.758LeuAla: 14.758 ± 0.134	0.765LeuCys: 0.765 ± 0.026	6.181LeuAsp: 6.181 ± 0.061	4.366LeuGlu: 4.366 ± 0.067	3.769LeuPhe: 3.769 ± 0.062	8.274LeuGly: 8.274 ± 0.078	1.803LeuHis: 1.803 ± 0.037	4.727LeuIle: 4.727 ± 0.07	2.86LeuLys: 2.86 ± 0.054	9.335LeuLeu: 9.335 ± 0.134	2.014LeuMet: 2.014 ± 0.041	2.214LeuAsn: 2.214 ± 0.04	5.502LeuPro: 5.502 ± 0.067	2.352LeuGln: 2.352 ± 0.041	6.765LeuArg: 6.765 ± 0.075	5.843LeuSer: 5.843 ± 0.075	5.459LeuThr: 5.459 ± 0.073	7.083LeuVal: 7.083 ± 0.072	1.275LeuTrp: 1.275 ± 0.034	1.966LeuTyr: 1.966 ± 0.034	0.001LeuXaa: 0.001 ± 0.001
Met	3.272MetAla: 3.272 ± 0.054	0.157MetCys: 0.157 ± 0.01	1.094MetAsp: 1.094 ± 0.029	1.07MetGlu: 1.07 ± 0.031	0.665MetPhe: 0.665 ± 0.021	1.76MetGly: 1.76 ± 0.035	0.401MetHis: 0.401 ± 0.016	1.357MetIle: 1.357 ± 0.032	0.888MetLys: 0.888 ± 0.025	2.566MetLeu: 2.566 ± 0.049	0.629MetMet: 0.629 ± 0.023	0.631MetAsn: 0.631 ± 0.022	1.42MetPro: 1.42 ± 0.03	0.709MetGln: 0.709 ± 0.02	1.853MetArg: 1.853 ± 0.037	1.356MetSer: 1.356 ± 0.031	1.893MetThr: 1.893 ± 0.044	1.494MetVal: 1.494 ± 0.034	0.243MetTrp: 0.243 ± 0.012	0.256MetTyr: 0.256 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.102AsnAla: 3.102 ± 0.05	0.257AsnCys: 0.257 ± 0.015	1.4AsnAsp: 1.4 ± 0.039	1.098AsnGlu: 1.098 ± 0.03	1.01AsnPhe: 1.01 ± 0.031	2.362AsnGly: 2.362 ± 0.057	0.469AsnHis: 0.469 ± 0.018	1.351AsnIle: 1.351 ± 0.034	0.588AsnLys: 0.588 ± 0.022	2.39AsnLeu: 2.39 ± 0.044	0.546AsnMet: 0.546 ± 0.019	0.642AsnAsn: 0.642 ± 0.022	1.754AsnPro: 1.754 ± 0.034	0.69AsnGln: 0.69 ± 0.023	1.894AsnArg: 1.894 ± 0.036	1.23AsnSer: 1.23 ± 0.039	0.976AsnThr: 0.976 ± 0.031	1.723AsnVal: 1.723 ± 0.041	0.45AsnTrp: 0.45 ± 0.019	0.712AsnTyr: 0.712 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.752ProAla: 7.752 ± 0.1	0.335ProCys: 0.335 ± 0.015	3.895ProAsp: 3.895 ± 0.055	3.439ProGlu: 3.439 ± 0.051	1.877ProPhe: 1.877 ± 0.039	4.894ProGly: 4.894 ± 0.063	1.058ProHis: 1.058 ± 0.026	2.385ProIle: 2.385 ± 0.043	1.548ProLys: 1.548 ± 0.036	4.988ProLeu: 4.988 ± 0.068	1.22ProMet: 1.22 ± 0.029	1.245ProAsn: 1.245 ± 0.031	2.975ProPro: 2.975 ± 0.064	1.576ProGln: 1.576 ± 0.032	3.207ProArg: 3.207 ± 0.048	2.687ProSer: 2.687 ± 0.039	2.519ProThr: 2.519 ± 0.052	4.327ProVal: 4.327 ± 0.056	0.708ProTrp: 0.708 ± 0.023	1.094ProTyr: 1.094 ± 0.027	0.002ProXaa: 0.002 ± 0.001
Gln	3.932GlnAla: 3.932 ± 0.055	0.218GlnCys: 0.218 ± 0.012	1.376GlnAsp: 1.376 ± 0.034	1.196GlnGlu: 1.196 ± 0.029	1.084GlnPhe: 1.084 ± 0.03	2.305GlnGly: 2.305 ± 0.037	0.55GlnHis: 0.55 ± 0.018	1.679GlnIle: 1.679 ± 0.037	0.886GlnLys: 0.886 ± 0.031	3.046GlnLeu: 3.046 ± 0.05	0.795GlnMet: 0.795 ± 0.025	0.763GlnAsn: 0.763 ± 0.024	1.696GlnPro: 1.696 ± 0.033	1.132GlnGln: 1.132 ± 0.033	2.437GlnArg: 2.437 ± 0.042	1.654GlnSer: 1.654 ± 0.035	1.497GlnThr: 1.497 ± 0.034	2.005GlnVal: 2.005 ± 0.038	0.467GlnTrp: 0.467 ± 0.02	0.646GlnTyr: 0.646 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.298ArgAla: 9.298 ± 0.089	0.528ArgCys: 0.528 ± 0.02	4.505ArgAsp: 4.505 ± 0.056	4.018ArgGlu: 4.018 ± 0.057	3.184ArgPhe: 3.184 ± 0.049	5.399ArgGly: 5.399 ± 0.073	1.75ArgHis: 1.75 ± 0.038	4.229ArgIle: 4.229 ± 0.061	2.171ArgLys: 2.171 ± 0.042	8.252ArgLeu: 8.252 ± 0.103	1.883ArgMet: 1.883 ± 0.036	1.765ArgAsn: 1.765 ± 0.036	3.812ArgPro: 3.812 ± 0.055	2.419ArgGln: 2.419 ± 0.045	6.063ArgArg: 6.063 ± 0.092	3.738ArgSer: 3.738 ± 0.056	3.53ArgThr: 3.53 ± 0.051	4.804ArgVal: 4.804 ± 0.064	1.308ArgTrp: 1.308 ± 0.033	1.974ArgTyr: 1.974 ± 0.038	0.001ArgXaa: 0.001 ± 0.001
Ser	6.419SerAla: 6.419 ± 0.082	0.411SerCys: 0.411 ± 0.019	3.33SerAsp: 3.33 ± 0.045	2.643SerGlu: 2.643 ± 0.046	2.186SerPhe: 2.186 ± 0.049	5.422SerGly: 5.422 ± 0.08	0.955SerHis: 0.955 ± 0.028	2.594SerIle: 2.594 ± 0.046	1.394SerLys: 1.394 ± 0.033	5.124SerLeu: 5.124 ± 0.062	1.18SerMet: 1.18 ± 0.032	1.339SerAsn: 1.339 ± 0.032	2.789SerPro: 2.789 ± 0.046	1.48SerGln: 1.48 ± 0.033	3.529SerArg: 3.529 ± 0.052	2.581SerSer: 2.581 ± 0.054	2.344SerThr: 2.344 ± 0.048	3.62SerVal: 3.62 ± 0.056	0.806SerTrp: 0.806 ± 0.023	1.345SerTyr: 1.345 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	6.463ThrAla: 6.463 ± 0.085	0.361ThrCys: 0.361 ± 0.016	2.995ThrAsp: 2.995 ± 0.048	2.283ThrGlu: 2.283 ± 0.041	1.618ThrPhe: 1.618 ± 0.036	5.22ThrGly: 5.22 ± 0.076	0.969ThrHis: 0.969 ± 0.026	2.934ThrIle: 2.934 ± 0.056	1.322ThrLys: 1.322 ± 0.035	5.581ThrLeu: 5.581 ± 0.069	1.208ThrMet: 1.208 ± 0.028	1.249ThrAsn: 1.249 ± 0.04	3.398ThrPro: 3.398 ± 0.057	1.332ThrGln: 1.332 ± 0.033	3.491ThrArg: 3.491 ± 0.049	2.577ThrSer: 2.577 ± 0.048	2.505ThrThr: 2.505 ± 0.051	3.81ThrVal: 3.81 ± 0.049	0.588ThrTrp: 0.588 ± 0.022	1.091ThrTyr: 1.091 ± 0.029	0.001ThrXaa: 0.001 ± 0.001
Val	9.858ValAla: 9.858 ± 0.096	0.51ValCys: 0.51 ± 0.02	4.462ValAsp: 4.462 ± 0.056	4.409ValGlu: 4.409 ± 0.061	2.081ValPhe: 2.081 ± 0.038	5.635ValGly: 5.635 ± 0.066	1.303ValHis: 1.303 ± 0.032	3.477ValIle: 3.477 ± 0.058	1.992ValLys: 1.992 ± 0.044	5.704ValLeu: 5.704 ± 0.078	1.531ValMet: 1.531 ± 0.035	1.904ValAsn: 1.904 ± 0.044	3.866ValPro: 3.866 ± 0.062	1.997ValGln: 1.997 ± 0.037	5.206ValArg: 5.206 ± 0.062	3.856ValSer: 3.856 ± 0.049	4.424ValThr: 4.424 ± 0.062	5.01ValVal: 5.01 ± 0.074	0.882ValTrp: 0.882 ± 0.025	1.359ValTyr: 1.359 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.483TrpAla: 1.483 ± 0.037	0.145TrpCys: 0.145 ± 0.01	0.805TrpAsp: 0.805 ± 0.025	0.607TrpGlu: 0.607 ± 0.02	0.588TrpPhe: 0.588 ± 0.021	1.017TrpGly: 1.017 ± 0.032	0.36TrpHis: 0.36 ± 0.015	0.733TrpIle: 0.733 ± 0.026	0.462TrpLys: 0.462 ± 0.02	1.874TrpLeu: 1.874 ± 0.043	0.372TrpMet: 0.372 ± 0.015	0.452TrpAsn: 0.452 ± 0.019	0.754TrpPro: 0.754 ± 0.025	0.617TrpGln: 0.617 ± 0.022	1.424TrpArg: 1.424 ± 0.036	0.965TrpSer: 0.965 ± 0.027	0.922TrpThr: 0.922 ± 0.024	0.914TrpVal: 0.914 ± 0.027	0.269TrpTrp: 0.269 ± 0.015	0.323TrpTyr: 0.323 ± 0.017	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.76TyrAla: 2.76 ± 0.044	0.232TyrCys: 0.232 ± 0.015	1.612TyrAsp: 1.612 ± 0.039	1.115TyrGlu: 1.115 ± 0.031	0.886TyrPhe: 0.886 ± 0.026	2.174TyrGly: 2.174 ± 0.045	0.513TyrHis: 0.513 ± 0.016	0.851TyrIle: 0.851 ± 0.025	0.6TyrLys: 0.6 ± 0.025	2.083TyrLeu: 2.083 ± 0.042	0.421TyrMet: 0.421 ± 0.016	0.624TyrAsn: 0.624 ± 0.023	1.079TyrPro: 1.079 ± 0.025	0.724TyrGln: 0.724 ± 0.024	2.024TyrArg: 2.024 ± 0.041	1.205TyrSer: 1.205 ± 0.033	1.001TyrThr: 1.001 ± 0.029	1.486TyrVal: 1.486 ± 0.031	0.389TyrTrp: 0.389 ± 0.017	0.672TyrTyr: 0.672 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4437 proteins (1429297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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