Soehngenia longivitae
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z0D9C6|A0A4Z0D9C6_9FIRM S-layer homology domain-containing protein OS=Soehngenia longivitae OX=2562294 GN=E4100_02430 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.28 KK3 pKa = 10.03 FLVMGLSLLLILSLAACSNNNGEE26 pKa = 4.46 VNEE29 pKa = 4.53 PDD31 pKa = 3.55 TPIDD35 pKa = 3.45 EE36 pKa = 4.66 TPIEE40 pKa = 4.38 NPDD43 pKa = 3.56 NGNVTEE49 pKa = 5.07 PEE51 pKa = 4.2 PEE53 pKa = 4.07 DD54 pKa = 5.15 DD55 pKa = 3.74 YY56 pKa = 11.75 TEE58 pKa = 4.31 EE59 pKa = 3.95 VTLYY63 pKa = 10.06 FANNDD68 pKa = 3.46 YY69 pKa = 11.39 VEE71 pKa = 4.62 TGNEE75 pKa = 3.94 EE76 pKa = 4.08 YY77 pKa = 10.72 DD78 pKa = 3.11 WLLEE82 pKa = 4.06 EE83 pKa = 4.5 KK84 pKa = 10.81 RR85 pKa = 11.84 EE86 pKa = 4.03 INYY89 pKa = 10.2 EE90 pKa = 3.98 GTTLEE95 pKa = 3.96 EE96 pKa = 3.82 AVIRR100 pKa = 11.84 EE101 pKa = 4.22 LMKK104 pKa = 11.17 GPVDD108 pKa = 3.84 TEE110 pKa = 4.0 NMSTGFPSTVKK121 pKa = 10.88 LIDD124 pKa = 3.35 VTVDD128 pKa = 3.12 EE129 pKa = 6.1 DD130 pKa = 3.6 KK131 pKa = 10.67 TCYY134 pKa = 10.81 VNFAQDD140 pKa = 3.38 GLYY143 pKa = 9.95 GGSMEE148 pKa = 4.3 EE149 pKa = 4.36 SYY151 pKa = 10.81 IIAQVVNSLVGLDD164 pKa = 3.37 SVEE167 pKa = 3.85 RR168 pKa = 11.84 VQFLIDD174 pKa = 3.78 GAPAEE179 pKa = 4.31 TLMGHH184 pKa = 6.6 ISIEE188 pKa = 4.16 EE189 pKa = 4.02 PFEE192 pKa = 4.52 SFDD195 pKa = 3.57 EE196 pKa = 4.44
Molecular weight: 21.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.706
IPC_protein 3.668
Toseland 3.478
ProMoST 3.783
Dawson 3.63
Bjellqvist 3.808
Wikipedia 3.503
Rodwell 3.49
Grimsley 3.389
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.872
Thurlkill 3.516
EMBOSS 3.528
Sillero 3.77
Patrickios 1.786
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A4Z0D8Y6|A0A4Z0D8Y6_9FIRM Iron-only hydrogenase system regulator OS=Soehngenia longivitae OX=2562294 GN=E4100_02080 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.2 KK9 pKa = 7.6 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.69 EE15 pKa = 3.5 HH16 pKa = 6.2 GFRR19 pKa = 11.84 KK20 pKa = 9.71 RR21 pKa = 11.84 MKK23 pKa = 8.42 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 AVLKK32 pKa = 10.08 ARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.76 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1787
0
1787
574980
37
1934
321.8
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.685 ± 0.057
0.794 ± 0.02
5.945 ± 0.05
7.761 ± 0.072
4.279 ± 0.043
6.362 ± 0.056
1.356 ± 0.021
10.237 ± 0.061
8.501 ± 0.058
9.453 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.022
6.056 ± 0.045
2.994 ± 0.028
2.306 ± 0.026
3.601 ± 0.035
6.065 ± 0.042
4.889 ± 0.041
6.448 ± 0.048
0.566 ± 0.017
4.159 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here