Capybara microvirus Cap3_SP_541

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5D5|A0A4P8W5D5_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_541 OX=2585472 PE=3 SV=1
MM1 pKa = 7.73SLEE4 pKa = 4.02SALIPLRR11 pKa = 11.84LLSGHH16 pKa = 5.98SANTSFSDD24 pKa = 3.2PGSPIKK30 pKa = 10.33QLYY33 pKa = 8.35HH34 pKa = 6.75GKK36 pKa = 10.27YY37 pKa = 9.8DD38 pKa = 3.86DD39 pKa = 3.92NGRR42 pKa = 11.84LTLVPDD48 pKa = 4.09GEE50 pKa = 4.78INLQEE55 pKa = 4.41QIDD58 pKa = 4.17SFRR61 pKa = 11.84DD62 pKa = 3.44QCDD65 pKa = 2.51MDD67 pKa = 4.24YY68 pKa = 10.6IIRR71 pKa = 11.84QLLQGNEE78 pKa = 4.25SVLSSRR84 pKa = 11.84QSLGYY89 pKa = 9.75IDD91 pKa = 3.52TTEE94 pKa = 4.46YY95 pKa = 9.2PSSMAALLQLRR106 pKa = 11.84IDD108 pKa = 4.13AEE110 pKa = 4.72SIFYY114 pKa = 10.43SLPLDD119 pKa = 3.86EE120 pKa = 5.44RR121 pKa = 11.84QHH123 pKa = 6.48FDD125 pKa = 2.69NSFDD129 pKa = 3.24VWFAQTGSPDD139 pKa = 3.14WLKK142 pKa = 11.04SISSSVSSSDD152 pKa = 3.15PAAPAAPAEE161 pKa = 4.49VISNEE166 pKa = 4.12SS167 pKa = 2.98

Molecular weight: 18.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVU5|A0A4V1FVU5_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_541 OX=2585472 PE=4 SV=1
MM1 pKa = 7.42SCYY4 pKa = 10.36SPNVVRR10 pKa = 11.84WSADD14 pKa = 3.01PFTGEE19 pKa = 4.72ASAQFLGQLRR29 pKa = 11.84DD30 pKa = 3.53QYY32 pKa = 11.24TYY34 pKa = 11.87DD35 pKa = 3.51HH36 pKa = 6.99LEE38 pKa = 4.0EE39 pKa = 3.99QAMQLVPCGQCIGCRR54 pKa = 11.84LDD56 pKa = 4.34YY57 pKa = 10.66SRR59 pKa = 11.84HH60 pKa = 4.39WADD63 pKa = 3.54RR64 pKa = 11.84LVLEE68 pKa = 4.31LSKK71 pKa = 11.59YY72 pKa = 10.07NGSALFITLTYY83 pKa = 10.92NDD85 pKa = 4.13DD86 pKa = 4.25HH87 pKa = 7.6LPLSDD92 pKa = 4.49DD93 pKa = 3.92FSVPTLLVRR102 pKa = 11.84DD103 pKa = 3.64TQLYY107 pKa = 9.7HH108 pKa = 6.32KK109 pKa = 9.54RR110 pKa = 11.84LRR112 pKa = 11.84KK113 pKa = 9.7AFPDD117 pKa = 3.03RR118 pKa = 11.84VLRR121 pKa = 11.84FFMCGEE127 pKa = 4.23YY128 pKa = 10.98GSITHH133 pKa = 6.84RR134 pKa = 11.84PHH136 pKa = 5.93YY137 pKa = 10.61HH138 pKa = 5.78EE139 pKa = 4.64VLFGLSMCDD148 pKa = 4.06FSDD151 pKa = 4.91LRR153 pKa = 11.84PCQRR157 pKa = 11.84NEE159 pKa = 3.57LGQLSFTSPTLEE171 pKa = 4.71KK172 pKa = 9.7IWSNGFILFSEE183 pKa = 4.36ISYY186 pKa = 11.46ASMCYY191 pKa = 9.54VSRR194 pKa = 11.84YY195 pKa = 7.49SLKK198 pKa = 10.72KK199 pKa = 10.45AFGYY203 pKa = 10.97SNDD206 pKa = 3.61PRR208 pKa = 11.84QHH210 pKa = 6.7PEE212 pKa = 3.48FLLMSRR218 pKa = 11.84RR219 pKa = 11.84PGIGLDD225 pKa = 3.43YY226 pKa = 10.91FEE228 pKa = 5.65SHH230 pKa = 7.49DD231 pKa = 3.67PTDD234 pKa = 3.43VVFFSGKK241 pKa = 9.81KK242 pKa = 8.66ISIPRR247 pKa = 11.84SVLDD251 pKa = 3.44RR252 pKa = 11.84CGDD255 pKa = 3.55SAFVSKK261 pKa = 10.84VLEE264 pKa = 3.89QRR266 pKa = 11.84QRR268 pKa = 11.84LAHH271 pKa = 6.38DD272 pKa = 3.56TLLTEE277 pKa = 5.68LSLTDD282 pKa = 5.32LPYY285 pKa = 10.86LDD287 pKa = 4.24YY288 pKa = 11.14IKK290 pKa = 11.02VKK292 pKa = 10.1EE293 pKa = 3.84RR294 pKa = 11.84SKK296 pKa = 11.25AEE298 pKa = 4.18KK299 pKa = 9.61IAKK302 pKa = 9.25LKK304 pKa = 10.54KK305 pKa = 10.4RR306 pKa = 11.84SDD308 pKa = 3.41LL309 pKa = 3.78

Molecular weight: 35.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1437

77

556

239.5

26.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.933 ± 2.048

1.253 ± 0.494

6.959 ± 0.803

3.967 ± 0.45

5.428 ± 0.733

6.054 ± 0.809

1.949 ± 0.437

4.384 ± 0.507

3.619 ± 0.472

8.838 ± 1.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.394

3.688 ± 0.613

5.498 ± 0.416

4.036 ± 0.449

5.08 ± 0.865

12.178 ± 1.551

5.08 ± 0.774

5.289 ± 0.527

1.253 ± 0.204

5.428 ± 0.564
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski