Amino acid dipepetide frequency for Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.523AlaAla: 4.523 ± 0.099
0.503AlaCys: 0.503 ± 0.028
2.78AlaAsp: 2.78 ± 0.067
4.033AlaGlu: 4.033 ± 0.083
3.479AlaPhe: 3.479 ± 0.081
4.605AlaGly: 4.605 ± 0.101
0.988AlaHis: 0.988 ± 0.04
5.541AlaIle: 5.541 ± 0.107
4.606AlaLys: 4.606 ± 0.09
6.999AlaLeu: 6.999 ± 0.122
1.72AlaMet: 1.72 ± 0.059
2.151AlaAsn: 2.151 ± 0.051
1.813AlaPro: 1.813 ± 0.051
1.481AlaGln: 1.481 ± 0.049
3.253AlaArg: 3.253 ± 0.075
3.493AlaSer: 3.493 ± 0.082
3.041AlaThr: 3.041 ± 0.073
5.093AlaVal: 5.093 ± 0.097
0.546AlaTrp: 0.546 ± 0.03
2.24AlaTyr: 2.24 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.446CysAla: 0.446 ± 0.029
0.064CysCys: 0.064 ± 0.011
0.472CysAsp: 0.472 ± 0.027
0.505CysGlu: 0.505 ± 0.028
0.319CysPhe: 0.319 ± 0.023
0.831CysGly: 0.831 ± 0.05
0.16CysHis: 0.16 ± 0.018
0.454CysIle: 0.454 ± 0.028
0.478CysLys: 0.478 ± 0.027
0.533CysLeu: 0.533 ± 0.028
0.186CysMet: 0.186 ± 0.017
0.305CysAsn: 0.305 ± 0.023
0.499CysPro: 0.499 ± 0.029
0.172CysGln: 0.172 ± 0.018
0.386CysArg: 0.386 ± 0.026
0.484CysSer: 0.484 ± 0.026
0.356CysThr: 0.356 ± 0.026
0.482CysVal: 0.482 ± 0.032
0.075CysTrp: 0.075 ± 0.011
0.28CysTyr: 0.28 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
2.894AspAla: 2.894 ± 0.075
0.379AspCys: 0.379 ± 0.028
2.347AspAsp: 2.347 ± 0.064
4.208AspGlu: 4.208 ± 0.07
3.03AspPhe: 3.03 ± 0.068
3.533AspGly: 3.533 ± 0.096
0.746AspHis: 0.746 ± 0.034
4.241AspIle: 4.241 ± 0.102
3.222AspLys: 3.222 ± 0.074
4.979AspLeu: 4.979 ± 0.087
1.13AspMet: 1.13 ± 0.039
2.033AspAsn: 2.033 ± 0.056
2.548AspPro: 2.548 ± 0.077
0.904AspGln: 0.904 ± 0.035
2.389AspArg: 2.389 ± 0.062
2.876AspSer: 2.876 ± 0.074
2.29AspThr: 2.29 ± 0.06
3.843AspVal: 3.843 ± 0.086
0.611AspTrp: 0.611 ± 0.034
2.298AspTyr: 2.298 ± 0.063
0.0AspXaa: 0.0 ± 0.0
Glu
4.882GluAla: 4.882 ± 0.102
0.434GluCys: 0.434 ± 0.029
3.674GluAsp: 3.674 ± 0.086
7.093GluGlu: 7.093 ± 0.142
3.509GluPhe: 3.509 ± 0.081
4.355GluGly: 4.355 ± 0.078
1.126GluHis: 1.126 ± 0.044
7.25GluIle: 7.25 ± 0.125
7.939GluLys: 7.939 ± 0.132
8.542GluLeu: 8.542 ± 0.144
2.006GluMet: 2.006 ± 0.056
4.054GluAsn: 4.054 ± 0.082
2.181GluPro: 2.181 ± 0.061
1.558GluGln: 1.558 ± 0.05
4.184GluArg: 4.184 ± 0.097
3.566GluSer: 3.566 ± 0.081
3.612GluThr: 3.612 ± 0.074
5.099GluVal: 5.099 ± 0.088
0.689GluTrp: 0.689 ± 0.032
2.746GluTyr: 2.746 ± 0.067
0.0GluXaa: 0.0 ± 0.0
Phe
3.067PheAla: 3.067 ± 0.079
0.406PheCys: 0.406 ± 0.025
2.907PheAsp: 2.907 ± 0.069
3.846PheGlu: 3.846 ± 0.077
2.759PhePhe: 2.759 ± 0.079
3.749PheGly: 3.749 ± 0.066
0.758PheHis: 0.758 ± 0.033
3.828PheIle: 3.828 ± 0.089
3.085PheLys: 3.085 ± 0.084
5.418PheLeu: 5.418 ± 0.108
1.18PheMet: 1.18 ± 0.047
1.894PheAsn: 1.894 ± 0.053
1.954PhePro: 1.954 ± 0.054
1.208PheGln: 1.208 ± 0.04
2.129PheArg: 2.129 ± 0.058
3.901PheSer: 3.901 ± 0.092
2.332PheThr: 2.332 ± 0.064
3.773PheVal: 3.773 ± 0.075
0.57PheTrp: 0.57 ± 0.033
1.88PheTyr: 1.88 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.323GlyAla: 4.323 ± 0.106
0.618GlyCys: 0.618 ± 0.031
3.118GlyAsp: 3.118 ± 0.064
4.746GlyGlu: 4.746 ± 0.078
3.729GlyPhe: 3.729 ± 0.084
4.421GlyGly: 4.421 ± 0.11
1.136GlyHis: 1.136 ± 0.046
6.693GlyIle: 6.693 ± 0.084
6.181GlyLys: 6.181 ± 0.099
6.065GlyLeu: 6.065 ± 0.106
1.945GlyMet: 1.945 ± 0.058
2.883GlyAsn: 2.883 ± 0.077
1.726GlyPro: 1.726 ± 0.056
1.314GlyGln: 1.314 ± 0.05
3.174GlyArg: 3.174 ± 0.076
3.81GlySer: 3.81 ± 0.069
3.693GlyThr: 3.693 ± 0.087
4.999GlyVal: 4.999 ± 0.1
0.799GlyTrp: 0.799 ± 0.038
3.117GlyTyr: 3.117 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.886HisAla: 0.886 ± 0.036
0.177HisCys: 0.177 ± 0.017
0.825HisAsp: 0.825 ± 0.034
1.063HisGlu: 1.063 ± 0.04
0.871HisPhe: 0.871 ± 0.039
1.222HisGly: 1.222 ± 0.046
0.359HisHis: 0.359 ± 0.027
1.219HisIle: 1.219 ± 0.044
0.871HisLys: 0.871 ± 0.032
1.582HisLeu: 1.582 ± 0.053
0.356HisMet: 0.356 ± 0.025
0.678HisAsn: 0.678 ± 0.029
0.937HisPro: 0.937 ± 0.038
0.323HisGln: 0.323 ± 0.023
0.793HisArg: 0.793 ± 0.036
0.943HisSer: 0.943 ± 0.037
0.778HisThr: 0.778 ± 0.037
1.036HisVal: 1.036 ± 0.041
0.165HisTrp: 0.165 ± 0.015
0.689HisTyr: 0.689 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.003IleAla: 6.003 ± 0.114
0.53IleCys: 0.53 ± 0.025
4.774IleAsp: 4.774 ± 0.088
6.503IleGlu: 6.503 ± 0.112
4.045IlePhe: 4.045 ± 0.093
5.487IleGly: 5.487 ± 0.098
1.202IleHis: 1.202 ± 0.042
6.226IleIle: 6.226 ± 0.108
5.539IleLys: 5.539 ± 0.091
8.156IleLeu: 8.156 ± 0.107
1.669IleMet: 1.669 ± 0.052
3.099IleAsn: 3.099 ± 0.079
3.936IlePro: 3.936 ± 0.076
1.799IleGln: 1.799 ± 0.049
3.657IleArg: 3.657 ± 0.079
5.65IleSer: 5.65 ± 0.107
4.321IleThr: 4.321 ± 0.081
6.361IleVal: 6.361 ± 0.115
0.784IleTrp: 0.784 ± 0.037
2.84IleTyr: 2.84 ± 0.073
0.0IleXaa: 0.0 ± 0.0
Lys
4.891LysAla: 4.891 ± 0.088
0.475LysCys: 0.475 ± 0.028
3.801LysAsp: 3.801 ± 0.08
6.994LysGlu: 6.994 ± 0.121
2.771LysPhe: 2.771 ± 0.066
4.894LysGly: 4.894 ± 0.082
1.27LysHis: 1.27 ± 0.046
6.951LysIle: 6.951 ± 0.117
7.427LysLys: 7.427 ± 0.124
7.617LysLeu: 7.617 ± 0.106
1.958LysMet: 1.958 ± 0.055
3.717LysAsn: 3.717 ± 0.081
2.627LysPro: 2.627 ± 0.064
1.567LysGln: 1.567 ± 0.049
4.117LysArg: 4.117 ± 0.095
3.889LysSer: 3.889 ± 0.084
3.919LysThr: 3.919 ± 0.085
5.523LysVal: 5.523 ± 0.097
0.719LysTrp: 0.719 ± 0.038
2.936LysTyr: 2.936 ± 0.069
0.0LysXaa: 0.0 ± 0.0
Leu
6.268LeuAla: 6.268 ± 0.101
0.723LeuCys: 0.723 ± 0.033
5.1LeuAsp: 5.1 ± 0.085
8.111LeuGlu: 8.111 ± 0.118
4.991LeuPhe: 4.991 ± 0.112
6.566LeuGly: 6.566 ± 0.104
1.454LeuHis: 1.454 ± 0.051
7.195LeuIle: 7.195 ± 0.11
9.201LeuLys: 9.201 ± 0.143
10.282LeuLeu: 10.282 ± 0.157
2.326LeuMet: 2.326 ± 0.059
4.286LeuAsn: 4.286 ± 0.081
4.011LeuPro: 4.011 ± 0.081
2.319LeuGln: 2.319 ± 0.065
4.987LeuArg: 4.987 ± 0.097
7.647LeuSer: 7.647 ± 0.144
4.844LeuThr: 4.844 ± 0.075
6.572LeuVal: 6.572 ± 0.107
0.975LeuTrp: 0.975 ± 0.043
3.389LeuTyr: 3.389 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
1.681MetAla: 1.681 ± 0.054
0.162MetCys: 0.162 ± 0.014
1.264MetAsp: 1.264 ± 0.044
1.732MetGlu: 1.732 ± 0.056
0.918MetPhe: 0.918 ± 0.043
1.636MetGly: 1.636 ± 0.05
0.265MetHis: 0.265 ± 0.022
2.064MetIle: 2.064 ± 0.065
2.491MetLys: 2.491 ± 0.07
2.362MetLeu: 2.362 ± 0.066
0.569MetMet: 0.569 ± 0.032
1.202MetAsn: 1.202 ± 0.043
0.828MetPro: 0.828 ± 0.037
0.443MetGln: 0.443 ± 0.023
1.325MetArg: 1.325 ± 0.047
1.31MetSer: 1.31 ± 0.039
1.183MetThr: 1.183 ± 0.038
1.811MetVal: 1.811 ± 0.058
0.171MetTrp: 0.171 ± 0.014
0.666MetTyr: 0.666 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
2.591AsnAla: 2.591 ± 0.064
0.367AsnCys: 0.367 ± 0.025
2.029AsnAsp: 2.029 ± 0.052
2.897AsnGlu: 2.897 ± 0.07
2.214AsnPhe: 2.214 ± 0.056
3.017AsnGly: 3.017 ± 0.07
0.653AsnHis: 0.653 ± 0.03
3.326AsnIle: 3.326 ± 0.073
2.605AsnLys: 2.605 ± 0.072
4.449AsnLeu: 4.449 ± 0.08
0.886AsnMet: 0.886 ± 0.037
1.543AsnAsn: 1.543 ± 0.046
2.296AsnPro: 2.296 ± 0.061
0.838AsnGln: 0.838 ± 0.037
2.006AsnArg: 2.006 ± 0.059
2.439AsnSer: 2.439 ± 0.069
1.907AsnThr: 1.907 ± 0.051
3.008AsnVal: 3.008 ± 0.066
0.499AsnTrp: 0.499 ± 0.025
1.729AsnTyr: 1.729 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.084ProAla: 2.084 ± 0.058
0.22ProCys: 0.22 ± 0.02
2.472ProAsp: 2.472 ± 0.068
4.228ProGlu: 4.228 ± 0.083
2.066ProPhe: 2.066 ± 0.058
2.943ProGly: 2.943 ± 0.078
0.648ProHis: 0.648 ± 0.026
2.768ProIle: 2.768 ± 0.066
2.442ProLys: 2.442 ± 0.062
3.455ProLeu: 3.455 ± 0.084
0.861ProMet: 0.861 ± 0.037
1.421ProAsn: 1.421 ± 0.049
1.262ProPro: 1.262 ± 0.048
0.856ProGln: 0.856 ± 0.039
1.508ProArg: 1.508 ± 0.055
2.112ProSer: 2.112 ± 0.056
1.835ProThr: 1.835 ± 0.058
3.195ProVal: 3.195 ± 0.064
0.403ProTrp: 0.403 ± 0.029
1.576ProTyr: 1.576 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
1.404GlnAla: 1.404 ± 0.046
0.145GlnCys: 0.145 ± 0.017
0.958GlnAsp: 0.958 ± 0.039
1.72GlnGlu: 1.72 ± 0.054
0.87GlnPhe: 0.87 ± 0.041
1.244GlnGly: 1.244 ± 0.043
0.391GlnHis: 0.391 ± 0.027
1.889GlnIle: 1.889 ± 0.052
1.943GlnLys: 1.943 ± 0.061
2.328GlnLeu: 2.328 ± 0.059
0.572GlnMet: 0.572 ± 0.029
0.961GlnAsn: 0.961 ± 0.038
0.713GlnPro: 0.713 ± 0.031
0.621GlnGln: 0.621 ± 0.035
1.192GlnArg: 1.192 ± 0.041
1.097GlnSer: 1.097 ± 0.042
0.972GlnThr: 0.972 ± 0.04
1.398GlnVal: 1.398 ± 0.052
0.237GlnTrp: 0.237 ± 0.018
0.816GlnTyr: 0.816 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.65ArgAla: 2.65 ± 0.066
0.415ArgCys: 0.415 ± 0.028
2.214ArgAsp: 2.214 ± 0.065
4.151ArgGlu: 4.151 ± 0.098
2.665ArgPhe: 2.665 ± 0.065
2.952ArgGly: 2.952 ± 0.065
0.765ArgHis: 0.765 ± 0.034
4.554ArgIle: 4.554 ± 0.076
4.623ArgLys: 4.623 ± 0.103
4.493ArgLeu: 4.493 ± 0.082
1.356ArgMet: 1.356 ± 0.041
2.187ArgAsn: 2.187 ± 0.061
1.427ArgPro: 1.427 ± 0.05
1.066ArgGln: 1.066 ± 0.043
2.702ArgArg: 2.702 ± 0.082
2.391ArgSer: 2.391 ± 0.051
2.316ArgThr: 2.316 ± 0.059
3.323ArgVal: 3.323 ± 0.071
0.503ArgTrp: 0.503 ± 0.029
2.189ArgTyr: 2.189 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
3.409SerAla: 3.409 ± 0.077
0.494SerCys: 0.494 ± 0.03
2.933SerAsp: 2.933 ± 0.07
4.419SerGlu: 4.419 ± 0.098
3.427SerPhe: 3.427 ± 0.076
4.758SerGly: 4.758 ± 0.092
0.957SerHis: 0.957 ± 0.039
4.767SerIle: 4.767 ± 0.09
4.205SerLys: 4.205 ± 0.097
6.268SerLeu: 6.268 ± 0.111
1.431SerMet: 1.431 ± 0.047
2.19SerAsn: 2.19 ± 0.065
2.457SerPro: 2.457 ± 0.071
1.491SerGln: 1.491 ± 0.047
3.097SerArg: 3.097 ± 0.079
3.753SerSer: 3.753 ± 0.088
2.955SerThr: 2.955 ± 0.072
4.093SerVal: 4.093 ± 0.079
0.684SerTrp: 0.684 ± 0.036
2.276SerTyr: 2.276 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
3.332ThrAla: 3.332 ± 0.07
0.352ThrCys: 0.352 ± 0.026
2.394ThrAsp: 2.394 ± 0.065
3.045ThrGlu: 3.045 ± 0.074
2.526ThrPhe: 2.526 ± 0.066
4.226ThrGly: 4.226 ± 0.086
0.838ThrHis: 0.838 ± 0.039
4.009ThrIle: 4.009 ± 0.08
2.822ThrLys: 2.822 ± 0.071
5.373ThrLeu: 5.373 ± 0.106
1.058ThrMet: 1.058 ± 0.043
1.731ThrAsn: 1.731 ± 0.055
2.401ThrPro: 2.401 ± 0.065
0.844ThrGln: 0.844 ± 0.035
2.27ThrArg: 2.27 ± 0.061
2.717ThrSer: 2.717 ± 0.062
2.591ThrThr: 2.591 ± 0.074
3.927ThrVal: 3.927 ± 0.094
0.397ThrTrp: 0.397 ± 0.027
1.779ThrTyr: 1.779 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.849ValAla: 4.849 ± 0.093
0.581ValCys: 0.581 ± 0.032
3.843ValAsp: 3.843 ± 0.092
5.871ValGlu: 5.871 ± 0.107
3.871ValPhe: 3.871 ± 0.081
4.574ValGly: 4.574 ± 0.094
1.111ValHis: 1.111 ± 0.045
6.009ValIle: 6.009 ± 0.093
5.28ValLys: 5.28 ± 0.098
7.21ValLeu: 7.21 ± 0.116
1.75ValMet: 1.75 ± 0.054
2.789ValAsn: 2.789 ± 0.067
2.862ValPro: 2.862 ± 0.068
1.546ValGln: 1.546 ± 0.055
3.187ValArg: 3.187 ± 0.074
4.856ValSer: 4.856 ± 0.08
3.394ValThr: 3.394 ± 0.086
6.05ValVal: 6.05 ± 0.125
0.641ValTrp: 0.641 ± 0.032
2.576ValTyr: 2.576 ± 0.07
0.0ValXaa: 0.0 ± 0.0
Trp
0.47TrpAla: 0.47 ± 0.029
0.082TrpCys: 0.082 ± 0.011
0.552TrpAsp: 0.552 ± 0.033
0.705TrpGlu: 0.705 ± 0.036
0.488TrpPhe: 0.488 ± 0.03
0.708TrpGly: 0.708 ± 0.035
0.192TrpHis: 0.192 ± 0.019
0.75TrpIle: 0.75 ± 0.041
1.008TrpLys: 1.008 ± 0.046
1.081TrpLeu: 1.081 ± 0.04
0.262TrpMet: 0.262 ± 0.02
0.557TrpAsn: 0.557 ± 0.03
0.304TrpPro: 0.304 ± 0.024
0.259TrpGln: 0.259 ± 0.019
0.454TrpArg: 0.454 ± 0.027
0.567TrpSer: 0.567 ± 0.037
0.445TrpThr: 0.445 ± 0.029
0.651TrpVal: 0.651 ± 0.032
0.172TrpTrp: 0.172 ± 0.017
0.386TrpTyr: 0.386 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.232TyrAla: 2.232 ± 0.058
0.359TyrCys: 0.359 ± 0.025
2.045TyrAsp: 2.045 ± 0.063
2.629TyrGlu: 2.629 ± 0.063
2.097TyrPhe: 2.097 ± 0.059
2.773TyrGly: 2.773 ± 0.068
0.758TyrHis: 0.758 ± 0.036
2.847TyrIle: 2.847 ± 0.065
2.094TyrLys: 2.094 ± 0.06
4.056TyrLeu: 4.056 ± 0.085
0.859TyrMet: 0.859 ± 0.037
1.617TyrAsn: 1.617 ± 0.048
1.573TyrPro: 1.573 ± 0.051
0.858TyrGln: 0.858 ± 0.031
2.132TyrArg: 2.132 ± 0.061
2.561TyrSer: 2.561 ± 0.067
1.846TyrThr: 1.846 ± 0.056
2.615TyrVal: 2.615 ± 0.065
0.455TyrTrp: 0.455 ± 0.03
1.629TyrTyr: 1.629 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2087 proteins (667979 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski