Candidatus Nasuia deltocephalinicola str. NAS-ALF

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Nasuia; Candidatus Nasuia deltocephalinicola

Average proteome isoelectric point is 8.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5SQE3|S5SQE3_9PROT Cytochrome c oxidase subunit I OS=Candidatus Nasuia deltocephalinicola str. NAS-ALF OX=1343077 GN=cyoB PE=3 SV=1
MM1 pKa = 7.06FNKK4 pKa = 9.72VILMGEE10 pKa = 3.77IAMDD14 pKa = 3.6PEE16 pKa = 4.04LRR18 pKa = 11.84YY19 pKa = 10.2FKK21 pKa = 10.37DD22 pKa = 3.18NSVITVIKK30 pKa = 10.64LITRR34 pKa = 11.84EE35 pKa = 4.09NINNIKK41 pKa = 10.09DD42 pKa = 4.13LQWHH46 pKa = 6.68RR47 pKa = 11.84IILKK51 pKa = 9.59EE52 pKa = 3.94KK53 pKa = 10.99NKK55 pKa = 9.98ILKK58 pKa = 10.14KK59 pKa = 10.83NMIILIEE66 pKa = 4.2GKK68 pKa = 9.76IKK70 pKa = 9.01TRR72 pKa = 11.84RR73 pKa = 11.84WMDD76 pKa = 3.26NVKK79 pKa = 10.4YY80 pKa = 10.87NYY82 pKa = 8.26ITEE85 pKa = 4.27IFADD89 pKa = 4.04NIKK92 pKa = 10.51ILDD95 pKa = 3.64NKK97 pKa = 10.55NYY99 pKa = 10.68NNYY102 pKa = 9.64KK103 pKa = 9.99NKK105 pKa = 10.42KK106 pKa = 8.87NDD108 pKa = 3.69YY109 pKa = 9.85IDD111 pKa = 4.21NDD113 pKa = 3.59EE114 pKa = 5.02KK115 pKa = 11.54EE116 pKa = 4.58EE117 pKa = 4.12NDD119 pKa = 3.9EE120 pKa = 3.94NDD122 pKa = 3.32NYY124 pKa = 11.29NLEE127 pKa = 4.27DD128 pKa = 4.08SEE130 pKa = 4.83YY131 pKa = 10.27EE132 pKa = 4.14NKK134 pKa = 10.11NSKK137 pKa = 9.57IDD139 pKa = 3.73EE140 pKa = 5.19LIDD143 pKa = 4.0DD144 pKa = 4.65EE145 pKa = 5.33KK146 pKa = 11.83DD147 pKa = 3.38NIITVGGDD155 pKa = 3.53TEE157 pKa = 4.34LKK159 pKa = 9.98EE160 pKa = 3.92YY161 pKa = 10.71SYY163 pKa = 11.81DD164 pKa = 3.62EE165 pKa = 5.27DD166 pKa = 4.39YY167 pKa = 11.35EE168 pKa = 4.19

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5SQC5|S5SQC5_9PROT 50S ribosomal protein L15 OS=Candidatus Nasuia deltocephalinicola str. NAS-ALF OX=1343077 GN=rplO PE=3 SV=1
MM1 pKa = 6.74QKK3 pKa = 9.54EE4 pKa = 4.12RR5 pKa = 11.84KK6 pKa = 9.5RR7 pKa = 11.84FFLFNKK13 pKa = 8.85YY14 pKa = 8.52YY15 pKa = 10.85CKK17 pKa = 10.73LKK19 pKa = 10.68LLKK22 pKa = 10.5NQLNKK27 pKa = 10.28IIINKK32 pKa = 9.88SNTKK36 pKa = 9.76EE37 pKa = 4.05YY38 pKa = 10.76YY39 pKa = 10.05SEE41 pKa = 3.95LLKK44 pKa = 11.37VNFKK48 pKa = 10.01IQKK51 pKa = 9.84LPRR54 pKa = 11.84NSNGTRR60 pKa = 11.84IRR62 pKa = 11.84NRR64 pKa = 11.84CYY66 pKa = 8.66ITGRR70 pKa = 11.84PRR72 pKa = 11.84GVLRR76 pKa = 11.84LFGLSRR82 pKa = 11.84SIIRR86 pKa = 11.84DD87 pKa = 3.18IAFKK91 pKa = 10.76GYY93 pKa = 10.1IPGILKK99 pKa = 10.64SSWW102 pKa = 2.91

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

137

0

137

34211

33

1343

249.7

29.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.365 ± 0.157

1.377 ± 0.079

3.107 ± 0.182

3.391 ± 0.24

10.052 ± 0.552

3.622 ± 0.193

0.982 ± 0.064

14.685 ± 0.269

14.715 ± 0.466

10.459 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.31 ± 0.081

11.923 ± 0.258

1.564 ± 0.106

1.225 ± 0.082

2.318 ± 0.115

6.302 ± 0.226

2.344 ± 0.137

2.616 ± 0.142

0.517 ± 0.053

6.127 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski