Bacillus phage CP-51

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Siminovitchvirus; Bacillus virus CP51

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068EMY9|A0A068EMY9_9CAUD Uncharacterized protein OS=Bacillus phage CP-51 OX=1391188 PE=4 SV=1
MM1 pKa = 6.97SQPYY5 pKa = 9.42QEE7 pKa = 4.41VPAVVQEE14 pKa = 3.98WLVNEE19 pKa = 3.77VRR21 pKa = 11.84GNIEE25 pKa = 3.48QHH27 pKa = 6.01NEE29 pKa = 3.34LMSNIEE35 pKa = 4.11EE36 pKa = 4.31PKK38 pKa = 10.49VVYY41 pKa = 9.83IDD43 pKa = 3.46SDD45 pKa = 3.38IYY47 pKa = 11.34SIVDD51 pKa = 3.68NILSSLHH58 pKa = 6.31EE59 pKa = 4.32SVITPNVNNRR69 pKa = 11.84FDD71 pKa = 3.95IEE73 pKa = 4.1EE74 pKa = 4.29DD75 pKa = 3.64CC76 pKa = 5.61

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068EUC6|A0A068EUC6_9CAUD Uncharacterized protein OS=Bacillus phage CP-51 OX=1391188 PE=4 SV=1
MM1 pKa = 7.2NVFLRR6 pKa = 11.84SVSACLKK13 pKa = 7.62TAPRR17 pKa = 11.84LQGTPSVLVLPHH29 pKa = 6.7GLLEE33 pKa = 4.25RR34 pKa = 11.84LGTIALPLLYY44 pKa = 10.15QAVHH48 pKa = 6.49KK49 pKa = 9.24PCPAPRR55 pKa = 11.84KK56 pKa = 9.42CSILIARR63 pKa = 11.84VYY65 pKa = 10.33RR66 pKa = 11.84IIRR69 pKa = 11.84ILRR72 pKa = 11.84VCCPKK77 pKa = 10.78DD78 pKa = 3.25IVLLL82 pKa = 4.17

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

44658

20

3618

202.1

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.445 ± 0.306

0.988 ± 0.085

6.523 ± 0.174

7.519 ± 0.325

3.395 ± 0.104

7.112 ± 0.209

2.152 ± 0.178

6.27 ± 0.125

8.028 ± 0.228

7.86 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.081 ± 0.179

5.119 ± 0.238

3.489 ± 0.114

3.363 ± 0.103

4.543 ± 0.147

5.645 ± 0.171

6.12 ± 0.3

6.977 ± 0.163

1.413 ± 0.067

3.959 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski