Amino acid dipepetide frequency for Bacillus phage CP-51

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.172AlaAla: 2.172 ± 0.3
0.649AlaCys: 0.649 ± 0.131
4.568AlaAsp: 4.568 ± 0.611
4.747AlaGlu: 4.747 ± 0.29
2.172AlaPhe: 2.172 ± 0.241
4.613AlaGly: 4.613 ± 0.405
1.344AlaHis: 1.344 ± 0.173
5.061AlaIle: 5.061 ± 0.281
5.643AlaLys: 5.643 ± 0.432
5.397AlaLeu: 5.397 ± 0.312
1.702AlaMet: 1.702 ± 0.204
3.628AlaAsn: 3.628 ± 0.402
2.351AlaPro: 2.351 ± 0.259
2.441AlaGln: 2.441 ± 0.431
2.956AlaArg: 2.956 ± 0.223
3.113AlaSer: 3.113 ± 0.341
3.807AlaThr: 3.807 ± 0.35
4.523AlaVal: 4.523 ± 0.297
0.963AlaTrp: 0.963 ± 0.224
2.598AlaTyr: 2.598 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.381CysAla: 0.381 ± 0.082
0.112CysCys: 0.112 ± 0.048
0.403CysAsp: 0.403 ± 0.1
0.672CysGlu: 0.672 ± 0.125
0.269CysPhe: 0.269 ± 0.087
0.717CysGly: 0.717 ± 0.124
0.179CysHis: 0.179 ± 0.071
0.851CysIle: 0.851 ± 0.147
0.829CysLys: 0.829 ± 0.111
1.03CysLeu: 1.03 ± 0.17
0.291CysMet: 0.291 ± 0.082
0.448CysAsn: 0.448 ± 0.098
0.425CysPro: 0.425 ± 0.11
0.269CysGln: 0.269 ± 0.074
0.515CysArg: 0.515 ± 0.088
0.717CysSer: 0.717 ± 0.128
0.627CysThr: 0.627 ± 0.141
0.582CysVal: 0.582 ± 0.134
0.179CysTrp: 0.179 ± 0.058
0.381CysTyr: 0.381 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
4.165AspAla: 4.165 ± 0.408
0.493AspCys: 0.493 ± 0.12
3.605AspAsp: 3.605 ± 0.279
4.747AspGlu: 4.747 ± 0.439
2.508AspPhe: 2.508 ± 0.234
4.814AspGly: 4.814 ± 0.392
1.523AspHis: 1.523 ± 0.317
4.814AspIle: 4.814 ± 0.278
5.531AspLys: 5.531 ± 0.37
5.733AspLeu: 5.733 ± 0.358
2.306AspMet: 2.306 ± 0.234
3.225AspAsn: 3.225 ± 0.212
2.172AspPro: 2.172 ± 0.339
1.702AspGln: 1.702 ± 0.209
2.508AspArg: 2.508 ± 0.272
3.426AspSer: 3.426 ± 0.314
3.717AspThr: 3.717 ± 0.261
4.076AspVal: 4.076 ± 0.303
1.366AspTrp: 1.366 ± 0.273
2.777AspTyr: 2.777 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
4.77GluAla: 4.77 ± 0.354
0.717GluCys: 0.717 ± 0.111
5.643GluAsp: 5.643 ± 0.379
7.233GluGlu: 7.233 ± 0.826
2.598GluPhe: 2.598 ± 0.267
5.912GluGly: 5.912 ± 0.403
1.859GluHis: 1.859 ± 0.271
4.568GluIle: 4.568 ± 0.255
5.016GluLys: 5.016 ± 0.441
6.225GluLeu: 6.225 ± 0.466
2.396GluMet: 2.396 ± 0.32
2.844GluAsn: 2.844 ± 0.232
1.971GluPro: 1.971 ± 0.253
3.001GluGln: 3.001 ± 0.263
3.09GluArg: 3.09 ± 0.316
3.426GluSer: 3.426 ± 0.301
3.202GluThr: 3.202 ± 0.256
5.822GluVal: 5.822 ± 0.492
1.411GluTrp: 1.411 ± 0.202
3.493GluTyr: 3.493 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
1.971PheAla: 1.971 ± 0.229
0.493PheCys: 0.493 ± 0.11
2.396PheAsp: 2.396 ± 0.218
2.06PheGlu: 2.06 ± 0.211
1.097PhePhe: 1.097 ± 0.188
2.284PheGly: 2.284 ± 0.271
0.739PheHis: 0.739 ± 0.152
1.724PheIle: 1.724 ± 0.205
2.889PheLys: 2.889 ± 0.283
2.687PheLeu: 2.687 ± 0.257
0.851PheMet: 0.851 ± 0.14
1.881PheAsn: 1.881 ± 0.222
1.344PhePro: 1.344 ± 0.199
1.097PheGln: 1.097 ± 0.158
1.926PheArg: 1.926 ± 0.201
2.553PheSer: 2.553 ± 0.209
2.374PheThr: 2.374 ± 0.24
2.06PheVal: 2.06 ± 0.201
0.448PheTrp: 0.448 ± 0.097
1.075PheTyr: 1.075 ± 0.141
0.0PheXaa: 0.0 ± 0.0
Gly
4.299GlyAla: 4.299 ± 0.287
0.649GlyCys: 0.649 ± 0.105
4.232GlyAsp: 4.232 ± 0.272
4.658GlyGlu: 4.658 ± 0.393
2.777GlyPhe: 2.777 ± 0.235
6.024GlyGly: 6.024 ± 0.695
1.254GlyHis: 1.254 ± 0.208
4.882GlyIle: 4.882 ± 0.322
5.733GlyLys: 5.733 ± 0.465
5.665GlyLeu: 5.665 ± 0.35
2.374GlyMet: 2.374 ± 0.275
4.098GlyAsn: 4.098 ± 0.467
0.224GlyPro: 0.224 ± 0.083
2.015GlyGln: 2.015 ± 0.278
3.292GlyArg: 3.292 ± 0.244
3.919GlySer: 3.919 ± 0.323
5.8GlyThr: 5.8 ± 0.675
5.397GlyVal: 5.397 ± 0.395
1.008GlyTrp: 1.008 ± 0.144
2.822GlyTyr: 2.822 ± 0.239
0.0GlyXaa: 0.0 ± 0.0
His
0.873HisAla: 0.873 ± 0.135
0.157HisCys: 0.157 ± 0.05
1.164HisAsp: 1.164 ± 0.189
1.321HisGlu: 1.321 ± 0.219
1.142HisPhe: 1.142 ± 0.214
1.612HisGly: 1.612 ± 0.222
0.493HisHis: 0.493 ± 0.133
1.523HisIle: 1.523 ± 0.239
1.478HisLys: 1.478 ± 0.228
1.769HisLeu: 1.769 ± 0.229
0.649HisMet: 0.649 ± 0.123
1.142HisAsn: 1.142 ± 0.134
1.12HisPro: 1.12 ± 0.174
0.761HisGln: 0.761 ± 0.127
1.097HisArg: 1.097 ± 0.186
1.187HisSer: 1.187 ± 0.164
1.164HisThr: 1.164 ± 0.159
1.433HisVal: 1.433 ± 0.162
0.381HisTrp: 0.381 ± 0.091
1.052HisTyr: 1.052 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
3.628IleAla: 3.628 ± 0.293
0.47IleCys: 0.47 ± 0.096
4.882IleAsp: 4.882 ± 0.313
5.15IleGlu: 5.15 ± 0.325
1.433IlePhe: 1.433 ± 0.162
3.807IleGly: 3.807 ± 0.284
1.299IleHis: 1.299 ± 0.21
3.583IleIle: 3.583 ± 0.315
5.195IleLys: 5.195 ± 0.343
4.434IleLeu: 4.434 ± 0.427
2.015IleMet: 2.015 ± 0.258
3.717IleAsn: 3.717 ± 0.323
2.754IlePro: 2.754 ± 0.236
2.396IleGln: 2.396 ± 0.235
3.381IleArg: 3.381 ± 0.284
3.359IleSer: 3.359 ± 0.413
4.479IleThr: 4.479 ± 0.4
3.941IleVal: 3.941 ± 0.323
0.493IleTrp: 0.493 ± 0.106
2.284IleTyr: 2.284 ± 0.205
0.0IleXaa: 0.0 ± 0.0
Lys
6.83LysAla: 6.83 ± 0.527
0.694LysCys: 0.694 ± 0.13
5.912LysAsp: 5.912 ± 0.404
7.076LysGlu: 7.076 ± 0.561
2.374LysPhe: 2.374 ± 0.268
5.665LysGly: 5.665 ± 0.403
2.06LysHis: 2.06 ± 0.262
3.605LysIle: 3.605 ± 0.286
6.561LysLys: 6.561 ± 0.605
5.733LysLeu: 5.733 ± 0.332
2.53LysMet: 2.53 ± 0.324
3.113LysAsn: 3.113 ± 0.273
3.292LysPro: 3.292 ± 0.249
2.911LysGln: 2.911 ± 0.24
3.314LysArg: 3.314 ± 0.39
5.016LysSer: 5.016 ± 0.452
3.874LysThr: 3.874 ± 0.325
5.755LysVal: 5.755 ± 0.285
1.052LysTrp: 1.052 ± 0.164
2.911LysTyr: 2.911 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
5.33LeuAla: 5.33 ± 0.4
0.806LeuCys: 0.806 ± 0.172
5.643LeuAsp: 5.643 ± 0.385
6.449LeuGlu: 6.449 ± 0.399
2.396LeuPhe: 2.396 ± 0.221
5.285LeuGly: 5.285 ± 0.348
1.523LeuHis: 1.523 ± 0.226
4.21LeuIle: 4.21 ± 0.325
6.248LeuLys: 6.248 ± 0.394
6.292LeuLeu: 6.292 ± 0.445
2.329LeuMet: 2.329 ± 0.319
3.807LeuAsn: 3.807 ± 0.303
3.135LeuPro: 3.135 ± 0.307
3.113LeuGln: 3.113 ± 0.334
3.449LeuArg: 3.449 ± 0.263
4.77LeuSer: 4.77 ± 0.398
4.949LeuThr: 4.949 ± 0.259
5.038LeuVal: 5.038 ± 0.491
1.052LeuTrp: 1.052 ± 0.131
2.777LeuTyr: 2.777 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
2.374MetAla: 2.374 ± 0.27
0.381MetCys: 0.381 ± 0.108
1.724MetAsp: 1.724 ± 0.198
2.239MetGlu: 2.239 ± 0.206
1.12MetPhe: 1.12 ± 0.161
2.374MetGly: 2.374 ± 0.326
0.425MetHis: 0.425 ± 0.113
1.59MetIle: 1.59 ± 0.184
2.642MetLys: 2.642 ± 0.311
2.486MetLeu: 2.486 ± 0.266
0.896MetMet: 0.896 ± 0.162
1.971MetAsn: 1.971 ± 0.205
0.784MetPro: 0.784 ± 0.126
1.052MetGln: 1.052 ± 0.141
1.679MetArg: 1.679 ± 0.23
2.172MetSer: 2.172 ± 0.205
1.635MetThr: 1.635 ± 0.204
1.769MetVal: 1.769 ± 0.201
0.381MetTrp: 0.381 ± 0.091
1.12MetTyr: 1.12 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
3.717AsnAla: 3.717 ± 0.606
0.537AsnCys: 0.537 ± 0.109
3.068AsnAsp: 3.068 ± 0.244
2.575AsnGlu: 2.575 ± 0.245
1.679AsnPhe: 1.679 ± 0.183
3.829AsnGly: 3.829 ± 0.399
1.097AsnHis: 1.097 ± 0.152
3.829AsnIle: 3.829 ± 0.25
4.389AsnLys: 4.389 ± 0.422
3.471AsnLeu: 3.471 ± 0.29
1.635AsnMet: 1.635 ± 0.211
2.956AsnAsn: 2.956 ± 0.354
2.463AsnPro: 2.463 ± 0.238
1.657AsnGln: 1.657 ± 0.209
2.396AsnArg: 2.396 ± 0.204
2.754AsnSer: 2.754 ± 0.228
3.09AsnThr: 3.09 ± 0.372
3.001AsnVal: 3.001 ± 0.246
0.851AsnTrp: 0.851 ± 0.136
2.195AsnTyr: 2.195 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
2.284ProAla: 2.284 ± 0.253
0.336ProCys: 0.336 ± 0.075
2.306ProAsp: 2.306 ± 0.254
3.381ProGlu: 3.381 ± 0.275
1.03ProPhe: 1.03 ± 0.158
1.612ProGly: 1.612 ± 0.214
0.918ProHis: 0.918 ± 0.186
2.262ProIle: 2.262 ± 0.191
2.978ProLys: 2.978 ± 0.265
2.687ProLeu: 2.687 ± 0.229
0.985ProMet: 0.985 ± 0.154
1.657ProAsn: 1.657 ± 0.226
1.008ProPro: 1.008 ± 0.191
1.03ProGln: 1.03 ± 0.146
1.254ProArg: 1.254 ± 0.172
2.127ProSer: 2.127 ± 0.185
2.777ProThr: 2.777 ± 0.226
2.418ProVal: 2.418 ± 0.253
0.425ProTrp: 0.425 ± 0.092
1.411ProTyr: 1.411 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
2.441GlnAla: 2.441 ± 0.342
0.269GlnCys: 0.269 ± 0.075
1.859GlnAsp: 1.859 ± 0.203
2.62GlnGlu: 2.62 ± 0.27
1.366GlnPhe: 1.366 ± 0.178
2.598GlnGly: 2.598 ± 0.287
0.829GlnHis: 0.829 ± 0.159
1.926GlnIle: 1.926 ± 0.229
2.508GlnLys: 2.508 ± 0.262
3.09GlnLeu: 3.09 ± 0.303
1.321GlnMet: 1.321 ± 0.2
1.344GlnAsn: 1.344 ± 0.156
1.321GlnPro: 1.321 ± 0.226
1.276GlnGln: 1.276 ± 0.181
1.433GlnArg: 1.433 ± 0.166
1.568GlnSer: 1.568 ± 0.197
1.321GlnThr: 1.321 ± 0.173
2.329GlnVal: 2.329 ± 0.219
0.448GlnTrp: 0.448 ± 0.088
1.769GlnTyr: 1.769 ± 0.183
0.0GlnXaa: 0.0 ± 0.0
Arg
2.687ArgAla: 2.687 ± 0.208
0.403ArgCys: 0.403 ± 0.103
2.463ArgAsp: 2.463 ± 0.242
3.471ArgGlu: 3.471 ± 0.348
1.859ArgPhe: 1.859 ± 0.173
2.956ArgGly: 2.956 ± 0.284
0.918ArgHis: 0.918 ± 0.128
3.381ArgIle: 3.381 ± 0.24
3.919ArgLys: 3.919 ± 0.366
3.56ArgLeu: 3.56 ± 0.318
1.747ArgMet: 1.747 ± 0.295
2.284ArgAsn: 2.284 ± 0.237
1.388ArgPro: 1.388 ± 0.154
1.209ArgGln: 1.209 ± 0.159
2.396ArgArg: 2.396 ± 0.248
1.926ArgSer: 1.926 ± 0.233
2.486ArgThr: 2.486 ± 0.216
4.031ArgVal: 4.031 ± 0.301
0.605ArgTrp: 0.605 ± 0.124
1.747ArgTyr: 1.747 ± 0.176
0.0ArgXaa: 0.0 ± 0.0
Ser
4.367SerAla: 4.367 ± 0.404
0.425SerCys: 0.425 ± 0.091
3.762SerAsp: 3.762 ± 0.303
3.09SerGlu: 3.09 ± 0.27
2.239SerPhe: 2.239 ± 0.211
4.053SerGly: 4.053 ± 0.345
1.12SerHis: 1.12 ± 0.173
4.031SerIle: 4.031 ± 0.349
4.77SerLys: 4.77 ± 0.309
4.053SerLeu: 4.053 ± 0.268
1.993SerMet: 1.993 ± 0.193
2.866SerAsn: 2.866 ± 0.262
1.926SerPro: 1.926 ± 0.21
1.948SerGln: 1.948 ± 0.215
2.127SerArg: 2.127 ± 0.288
3.605SerSer: 3.605 ± 0.266
3.449SerThr: 3.449 ± 0.279
3.605SerVal: 3.605 ± 0.257
0.784SerTrp: 0.784 ± 0.138
2.239SerTyr: 2.239 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
3.964ThrAla: 3.964 ± 0.561
0.56ThrCys: 0.56 ± 0.114
3.135ThrAsp: 3.135 ± 0.274
4.456ThrGlu: 4.456 ± 0.286
2.172ThrPhe: 2.172 ± 0.241
4.994ThrGly: 4.994 ± 0.411
1.209ThrHis: 1.209 ± 0.164
3.583ThrIle: 3.583 ± 0.286
4.635ThrLys: 4.635 ± 0.25
4.501ThrLeu: 4.501 ± 0.301
1.388ThrMet: 1.388 ± 0.163
3.628ThrAsn: 3.628 ± 0.535
3.135ThrPro: 3.135 ± 0.267
1.568ThrGln: 1.568 ± 0.183
2.374ThrArg: 2.374 ± 0.22
3.426ThrSer: 3.426 ± 0.393
4.098ThrThr: 4.098 ± 0.591
5.374ThrVal: 5.374 ± 0.475
0.605ThrTrp: 0.605 ± 0.11
2.396ThrTyr: 2.396 ± 0.246
0.0ThrXaa: 0.0 ± 0.0
Val
4.658ValAla: 4.658 ± 0.359
0.761ValCys: 0.761 ± 0.168
4.949ValAsp: 4.949 ± 0.307
5.464ValGlu: 5.464 ± 0.493
1.814ValPhe: 1.814 ± 0.189
4.389ValGly: 4.389 ± 0.354
1.612ValHis: 1.612 ± 0.2
4.21ValIle: 4.21 ± 0.4
5.352ValLys: 5.352 ± 0.304
5.262ValLeu: 5.262 ± 0.365
1.679ValMet: 1.679 ± 0.169
3.493ValAsn: 3.493 ± 0.337
2.777ValPro: 2.777 ± 0.236
2.306ValGln: 2.306 ± 0.267
3.762ValArg: 3.762 ± 0.283
4.411ValSer: 4.411 ± 0.358
4.456ValThr: 4.456 ± 0.41
4.68ValVal: 4.68 ± 0.351
0.873ValTrp: 0.873 ± 0.152
2.866ValTyr: 2.866 ± 0.199
0.0ValXaa: 0.0 ± 0.0
Trp
0.985TrpAla: 0.985 ± 0.134
0.314TrpCys: 0.314 ± 0.105
0.941TrpAsp: 0.941 ± 0.136
1.164TrpGlu: 1.164 ± 0.156
0.493TrpPhe: 0.493 ± 0.106
1.142TrpGly: 1.142 ± 0.149
0.224TrpHis: 0.224 ± 0.056
0.672TrpIle: 0.672 ± 0.152
0.941TrpLys: 0.941 ± 0.156
0.918TrpLeu: 0.918 ± 0.169
0.605TrpMet: 0.605 ± 0.114
0.806TrpAsn: 0.806 ± 0.127
0.0TrpPro: 0.0 ± 0.0
0.56TrpGln: 0.56 ± 0.103
0.537TrpArg: 0.537 ± 0.1
0.851TrpSer: 0.851 ± 0.124
0.896TrpThr: 0.896 ± 0.167
1.366TrpVal: 1.366 ± 0.156
0.358TrpTrp: 0.358 ± 0.084
0.358TrpTyr: 0.358 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.553TyrAla: 2.553 ± 0.281
0.649TyrCys: 0.649 ± 0.112
2.575TyrAsp: 2.575 ± 0.227
2.62TyrGlu: 2.62 ± 0.25
1.411TyrPhe: 1.411 ± 0.169
2.441TyrGly: 2.441 ± 0.278
0.896TyrHis: 0.896 ± 0.139
2.396TyrIle: 2.396 ± 0.218
3.023TyrLys: 3.023 ± 0.223
3.605TyrLeu: 3.605 ± 0.326
1.097TyrMet: 1.097 ± 0.135
2.284TyrAsn: 2.284 ± 0.291
1.299TyrPro: 1.299 ± 0.191
1.321TyrGln: 1.321 ± 0.137
1.948TyrArg: 1.948 ± 0.167
2.127TyrSer: 2.127 ± 0.213
3.001TyrThr: 3.001 ± 0.395
2.575TyrVal: 2.575 ± 0.231
0.448TyrTrp: 0.448 ± 0.12
1.321TyrTyr: 1.321 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 221 proteins (44658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski