Pseudoruegeria lutimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pseudoruegeria

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9P111|A0A1G9P111_9RHOB Capsular exopolysaccharide family OS=Pseudoruegeria lutimaris OX=571298 GN=SAMN04488026_11326 PE=4 SV=1
MM1 pKa = 7.43GCSLWRR7 pKa = 11.84LQLMRR12 pKa = 11.84NLVDD16 pKa = 4.23TISLDD21 pKa = 3.43EE22 pKa = 4.81EE23 pKa = 4.61IFVEE27 pKa = 4.27TGDD30 pKa = 3.84VVTVEE35 pKa = 4.58PVNSEE40 pKa = 4.56DD41 pKa = 3.31ILAVSTDD48 pKa = 3.17QDD50 pKa = 4.05DD51 pKa = 4.17YY52 pKa = 11.89APCDD56 pKa = 3.52TGEE59 pKa = 4.28TTASGLDD66 pKa = 3.26VGGTVIFKK74 pKa = 10.03IKK76 pKa = 10.2HH77 pKa = 4.99VSDD80 pKa = 3.99PGGDD84 pKa = 3.37RR85 pKa = 11.84IYY87 pKa = 10.33GTSDD91 pKa = 2.35IYY93 pKa = 10.71GTSDD97 pKa = 3.63GEE99 pKa = 4.31DD100 pKa = 3.22SAHH103 pKa = 6.84YY104 pKa = 10.63SFTDD108 pKa = 3.33RR109 pKa = 11.84ASYY112 pKa = 11.23NLDD115 pKa = 2.75QWKK118 pKa = 10.44NGSDD122 pKa = 3.43TCHH125 pKa = 7.08VDD127 pKa = 2.95IVV129 pKa = 3.62

Molecular weight: 14.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9JH78|A0A1G9JH78_9RHOB 30S ribosomal protein S12 OS=Pseudoruegeria lutimaris OX=571298 GN=rpsL PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5650

0

5650

1678539

24

2797

297.1

32.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.88 ± 0.043

0.953 ± 0.011

5.729 ± 0.027

6.428 ± 0.033

3.849 ± 0.022

8.615 ± 0.032

2.06 ± 0.015

5.285 ± 0.024

3.165 ± 0.026

10.124 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.017

2.663 ± 0.016

5.117 ± 0.024

3.011 ± 0.015

6.774 ± 0.033

5.456 ± 0.024

5.253 ± 0.019

7.233 ± 0.026

1.414 ± 0.015

2.213 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski