Bacillus phage vB_BthS_BMBphi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494RBA9|A0A494RBA9_9CAUD Uncharacterized protein OS=Bacillus phage vB_BthS_BMBphi OX=2498843 GN=BMBphi_gp049 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 5.31KK3 pKa = 11.12DD4 pKa = 3.63KK5 pKa = 11.32ALEE8 pKa = 3.98MYY10 pKa = 10.73LRR12 pKa = 11.84GDD14 pKa = 3.58GKK16 pKa = 10.86LVEE19 pKa = 4.29CHH21 pKa = 6.12KK22 pKa = 11.12CGDD25 pKa = 3.54ILVVDD30 pKa = 4.65KK31 pKa = 11.37SWVVCLNEE39 pKa = 3.89EE40 pKa = 4.15CDD42 pKa = 3.91YY43 pKa = 11.55QLVTLEE49 pKa = 4.57GGLNNEE55 pKa = 4.43DD56 pKa = 3.92TEE58 pKa = 5.09SNN60 pKa = 3.44

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494RBE8|A0A494RBE8_9CAUD HNHc domain-containing protein OS=Bacillus phage vB_BthS_BMBphi OX=2498843 GN=BMBphi_gp078 PE=4 SV=1
MM1 pKa = 7.44NIVLVKK7 pKa = 9.69CQRR10 pKa = 11.84GYY12 pKa = 9.96IGEE15 pKa = 4.23RR16 pKa = 11.84MGSFTALRR24 pKa = 11.84IVHH27 pKa = 6.74LGEE30 pKa = 3.83TQEE33 pKa = 4.51RR34 pKa = 11.84IEE36 pKa = 4.1MMINVNLKK44 pKa = 9.42RR45 pKa = 11.84CMAKK49 pKa = 9.19MLCTLVIFCQGIYY62 pKa = 9.77EE63 pKa = 4.26KK64 pKa = 10.5PFRR67 pKa = 11.84AFKK70 pKa = 10.53RR71 pKa = 11.84HH72 pKa = 5.9LGTYY76 pKa = 9.38

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14365

33

873

181.8

20.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.896 ± 0.434

0.912 ± 0.106

5.875 ± 0.236

8.249 ± 0.324

4.107 ± 0.166

6.509 ± 0.31

1.873 ± 0.177

6.551 ± 0.159

8.472 ± 0.337

7.748 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.153

5.437 ± 0.276

3.223 ± 0.211

3.655 ± 0.207

4.859 ± 0.227

5.91 ± 0.238

5.882 ± 0.265

6.182 ± 0.242

1.462 ± 0.133

4.337 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski